Resource data sheet
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CSII-EF-hOct3/4-IRES2-Venus

Lentivirus vector construct of human OCT3/4 for generating iPS cells.

Catalog number RDB12908
Resource name CSII-EF-hOct3/4-IRES2-Venus
Clone info. Lentivirus expression vector of human OCT3/4 for generating iPS cells.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human POU5F1 cDNA
Originator Miyoshi, Hiroyuki |
 
Other clones in our bank human POU5F1 (NCBI Gene 5460) | human POU5F1 (NCBI Gene 5460) |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12908 CSII-EF-hOct3/4-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12908z.seq from Depositor
Electronic file PDF RDB12908.pdf from Depositor

Featured content

References

original Fujioka, T., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12908_A4Jfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 RDB12908_A4Jfa.seq
>12908_12908_A4Jf_pAxEF_F2_1_D02_11.ab1
    1 ATCTTCATTT AGTGTCGTGA CACGCTACCG GTCTCGAGAA TTCTGCAGCG GCCATCACAA
   61 GTTTGTACAA AAAAGCAGGC TCCGCGGCCG CCCCCTTCAC CATGGCGGGA CACCTGGCTT
  121 CGGATTTCGC CTTCTCGCCC CCTCCAGGTG GTGGAGGTGA TGGGCCAGGG GGGCCGGAGC
  181 CGGGCTGGGT TGATCCTCGG ACCTGGCTAA GCTTCCAAGG CCCTCCTGGA GGGCCAGGAA
  241 TCGGGCCGGG GGTTGGGCCA GGCTCTGAGG TGTGGGGGAT TCCCCCATGC CCCCCGCCGT
  301 ATGAGTTCTG TGGGGGGATG GCGTACTGTG GGCCCCAGGT TGGAGTGGGG CTAGTGCCCC
  361 AAGGCGGCTT GGAGACCTCT CAGCCTGAGG GCGAAGCAGG AGTCGGGGTG GAGAGCAACT
  421 CCGATGGGGC CTCCCCGGAG CCCTGCACCG TCACCCCTGG TGCCGTGAAG CTGGAGAAGG
  481 AGAAGCTGGA GCAAAACCCG GAGGAGTCCC AGGACATCAA AGCTCTGCAG AAAGAACTCG
  541 AGCAATTTGC CAAGCTCCTG AAGCAGAAGA GGATCACCCT GGGATATACA CAGGCCGATG
  601 TGGGGCTCAC CCTGGGGGTT CTATTTGGGA AGGTATTCAG CCAAACGACC ATCTGCCGCT
  661 TTGAGGCTCT GCAGCTTAGC TTCAAGAACA TGTGTAAGCT GCGGCCCTTG CTGCAGAAGT
  721 GGGTGGAGGA AGCTGACAAC AATGAAAATC TTCAGGAGAT ATGCAAAGCA GAAACCCTCG
  781 TGCAGGCCCG AAAGAGAAAG CGAACCAGTA TCGAGAACCG AGTGAGAGGC AACCTGGAGA
  841 ATTTGTTCCT GCAGTGCCCG AAACCCACAC TGCAGCAGAT CAGCCACATC GCCCAGCAGC
  901 TTGGGCTCGA GAAGGATGTG GTCCGAGTGT GGGTTCTGTA ACCGGCGCCA GAAGGCAAGC
  961 GATCAAGCAG CGACTATGCA CAACGAGAGG ATTTTGAGGC TGCTGGGTCT CCTTTTCTCA
 1021 GGGGGACAGT GTCCTTTTCT CTGGGCCCCA GGACCCATTT TGCTACCCCA GCCTATGGCA
1081 GTCCGTTCAC TGCACTGGCA CTGTACTCCC TTCGGTTCCC A
//
Primer: IRES-R RDB12908_A4Jfb.seq
>12908_12908_A4Jf_IRES_R_1_H02_23.ab1
    1 GCAAAACCGG GCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG
   61 CCATCACCAC TTTGTACAAG AAAGCTGGGT CGGCGCGCCC ACCCTTACCT CAGTTTGAAT
  121 GCATGGGAGA GCCCAGAGTG GTGACGGAGA CAGGGGGAAA GGCTTCCCCC TCAGGGAAAG
  181 GGACCGAGGA GTACAGTGCA GTGAAGTGAG GGCTCCCATA GCCTGGGGTA CCAAAATGGG
  241 GCCCTGGGGC CAGAGGAAAG GACACTGGTC CCCCTGAGAA AGGAGACCCA GCAGCCTCAA
  301 AATCCTCTCG TTGTGCATAG TCGCTGCTTG ATCGCTTGCC CTTCTGGCGC CGGTTACAGA
  361 ACCACACTCG GACCACATCC TTCTCGAGCC CAAGCTGCTG GGCGATGTGG CTGATCTGCT
  421 GCAGTGTGGG TTTCGGGCAC TGCAGGAACA AATTCTCCAG GTTGCCTCTC ACTCGGTTCT
  481 CGATACTGGT TCGCTTTCTC TTTCGGGCCT GCACGAGGGT TTCTGCTTTG CATATCTCCT
  541 GAAGATTTTC ATTGTTGTCA GCTTCCTCCA CCCACTTCTG CAGCAAGGGC CGCAGCTTAC
  601 ACATGTTCTT GAAGCTAAGC TGCAGAGCCT CAAAGCGGCA GATGGTCGTT TGGCTGAATA
  661 CCTTCCCAAA TAGAACCCCC AGGGTGAGCC CCACATCGGC CTGTGTATAT CCCAGGGTGA
  721 TCCTCTTCTG CTTCAGGAGC TTGGCAAATT GCTCGAGTTC TTTCTGCAGA GCTTTGATGT
  781 CCTGGGACTC CTCCGGGTTT TGCTCCAGCT TCTCCTTCTC CAGCTTCACG GCACCAGGGG
  841 TGACGGTGCA GGGCTCCGGG GAGGCCCCAT CGGAGTTGCT CTCCACCCCG ACTCCTGCTT
  901 CGCCCTCAGG CTGAGAGGTC TCCAAGCCGC CTTGGGGCAC TAGCCCCACT CCAACCTGGG
  961 GCCCACAGTA CGCCATCCCC CCACAGAACT CATACGGCGG GGGGGCATGG GGGGAATCCC
 1021 CACACTCAGA GCCTGACCAA CCCCCGGGCC GATTCCTGGT CCTCCAGGAG GGCTTGAGCT
 1081 AAGCAGGATC CGAGGATCAC CAAGCCGCTT CCGACTCCTT GACCATTCAC TTCAACCATC
1141 CTTGG
//
Primer: IRES-F RDB12908_A4Jfc.seq
>12908_12908_A4Jf-IRES_F_1_E02_14.ab1
    1 GCCTTCCTTT TCATGTGTTT AGTTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG
   61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG
  121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
  181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
  241 TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC
  301 TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT
  361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG
  421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG
  481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA
  541 ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA
  601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC
  661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC
  721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG
  781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGAA TTCAAGGCCT
  841 CTCGAGCCTC TAGAGTTAAA CATCGAGGGA TCAAGCTTAT CGATAATCAA CCTCTGGATT
  901 ACAAAATTTG TGAAAGATTG ACTGGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT
  961 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC
 1021 TCCTCCTTGT ATAATCCTGG GTTGCTGTCT CTTATGAGAG GTTGTGGCCC GTTGTCAGCC
 1081 ACGTGGCCGT ATGTGCACTG TGTTTGGCTG ACGCACCCCC CCACTGTTGG GGCCATTGGC
1141 CAACCCCA
//
Primer: CMV-Forward RDB12908_A4Jfd.seq
>12908_12908_A4Jf_CMV_Forward_1_C02_08.ab1
    1 CGTTAAACTC GGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
  121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
  181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
  241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
  301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
  361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
  421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
  481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
  541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
  601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
  661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
  721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG
  781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG
  901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA
  961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTATTTGTC
 1021 TGGGTATAGT GCAGCAGCCA GAACAATTTG CTGAAGGGGC TATTGAGCGC AACCAGCCAT
1081 CTGGTTGGCA CTCACAGTCT GGGGGCATCC AAGCAGCTCC AAGGGCCAAA GGA
//
Primer: BGH_rev RDB12908_A4Jfe.seq
>12908_12908_A4Jf_BGH_rev_1_F02_17.ab1
    1 GCTAGACGGG ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
   61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
  241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
  301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
  361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
  421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
  481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
  541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
  601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
  661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
  721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
  781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
  841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG
  901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC
  961 AGCGGGCAAA GGCAGGCGGC GATGAGTTCC GCCGTGGCAA TAGGGAGGGG GAAAGCGAAA
 1021 GTCCCGGGAA GGAGCTGACA GGTGGTGGCA ATGCCCCAAC CAGTGGGGGG TTGCGTCAGC
 1081 AAACACAGTG CACACACGTC ACGATTGCTG ACACGGACAC ACTTCTTCAT AAGAGACAGC
1141 CAACCAAGGG ATTTTATTAC A
//
Primer: SV40pro_F RDB12908_A4Jff.seq
>12908_12908_A4JF_SV40pro_F_1_G02_20.ab1
    1 GCTTGGTCCT GGACATCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
  901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGGTGCC TAATGAGTGA GCTAACTCAC
  961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
 1021 TTAATGAAAT CGGCCCAACG GCGCGGGGGA GAGGCGGTTT GCGTATTGGG CGGCTCTTCG
 1081 CCTTCTCGCT CACTGAACTC GCTGCGCTCG GATCGATCGG CTGCGGCGAG TCGGATCAGC
1141 TCCATCATAG GGCTCGGTTT A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fujioka, T., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836.
user report Hayashi, R., Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. PLoS One 7 (9): e45435 (2012). PMID 23029008.

2018.10.22

GNP_filter3_RDBDEP_html_181012.pl