Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-EVBsd

Lentivirus vector plasmid of Venus fluorescent protein and BSD tethered with T2A driven by EF-1 alpha promoter.

Sequence (full)	RDB12867zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12867_B2Abp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,Venus 5' Sequence file: RDB12867_B2Aba.seq
>12867_12867_B2Ab_3_EF1a-C_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNTNTT NAGTTTGGNN CTTGGTTNNT TCTCNAGCCT CAGACAGTGG    61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAATTAA TTAACCGGTT CCACAACCAT   121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG   181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG   241 CAAGCTGACC CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT   301 CGTGACCACC CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA   361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT   421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT   481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA   541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCACC GCCGACNAGC AGAAGAACGG   601 CATCNAGGCC AACTTCNAGA TCCGCCACNA CATCGAGGAN GGCNGCGTGC AGCTCGCCGA   661 NCACTACCAG CAGAACACCC CCNTCNGCGA CNGCCCNNNG CTGCTGNCCN NNNACCNCNA 721 NNNNNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,BSD,T2A,Venus 3' Sequence file: RDB12867_B2Abb.seq
>12867_12867_B2Ab_3_PRE_rev_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NAAANNNNNN NNNNNNNNNT AAGAATACCN GTCAATCTTT    61 CNCAAATTTT GTAATCCAGA GGTTGATTAT CGATAAGCTT GATCCCTCGA GTGCGGCCTT   121 AGCCCTCCCA CACATAACCA GAGGGCAGCA ATTCACGAAT CCCAACTGCC GTCGGCTGTC   181 CATCACTGTC CTTCACTATG GCTTTGATCC CAGGATGCAG ATCGAGAAGC ACCTGTCGGC   241 ACCGTCCGCA GGGGCTCAAG ATGCCCCTGT TCTCATTTCC GATCGCGACG ATACAAGTCA   301 GGTTGCCAGC TGCCGCAGCA GCAGCAGTGC CCAGCACCAC GAGTTCTGCA CAAGGTCCCC   361 CAGTAAAATG ATATACATTG ACACCAGTGA AGATGCGGCC GTCGCTAGAG AGAGCTGCGC   421 TGGCGACGCT GTAGTCTTCA GAGATGGGGA TGCTGTTGAT TGTAGCCGTT GCTCTTTCAA   481 TGAGGGTGGA TTCTTCTTGA GACAAAGGCT TGGCCATGGG CCCGGGATTT TCCTCCACGT   541 CACCGCATGT TAGAAGACTT CCTCTGCCCT CGCCGGATCC CTTGTACAGC TCGTCCATGC   601 CGAGAGTGAT CCCNGCGGCG GTCACNAACT CNNGCNNGAC CNTGTGATCG CGCTTCTCNT   661 NGGGGTCTTT GCTCNNNNNN NACNGGNANC TCNNNNNNNN NGNCNGNNNN CANCNNNGGN 721 NCNTCNNNAN NGGGGNNNNC NGNNNNNNAN NNNNNNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,attB2,attB1,cPPT_CTS Sequence file: RDB12867_B2Abc.seq
>12867_12867_B2Ab_3_EF1a-N_R_C06_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGGNCGA NGTGCGCTCT GCCCACTGAC GGGCACCGGA    61 GCCTCACGAA GCTTGAATTA TCACCACTTT GTACAAGAAA GCTGGGTCTA GATATCTCGA   121 GTGCGGCCGC GAATTCGGTA CCGGATCCAG TCGACTGAAT TGGTTCCTTT AAAGCCTGCT   181 TTTTTGTACA AACTTGTGAT AATTCAAAGT GAATTTCTGC TGTCCCTGTA ATAAACCCGA   241 AAATTTTGAA TTTTTGTAAT TTGTTTTTGT AATTCTTTAG TTTGTATGTC TGTTGCTATT   301 ATGTCTACTA TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG   361 TGGATGAATA CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT   421 CTCTCCACCT TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG   481 GGTCTGAAAC GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC   541 AAAAACTATT CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA   601 CAGCCAATTT GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA   661 TTCTTGTTCA TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT   721 TGTGTAATTG TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC NAATCTGTTC   781 CAGAGATTTA TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC   841 ANANCANNCC NNAATCCCCA GGANCTGTTG ATNCTTTAGG TATCTTTCNN CAGCCAGGAT   901 TCTNGNCTGN ANCTGCTTGA TGNCCCANAN TGTGAGTTGC AACANATGCT GTTGCGCCTC   961 NNNANCCNNC ANCAAATTGN TCTGNTGCTG CNNNANNNNN GANNATAATT GNCTGGNNNG 1021 NACCNNCAGC GNNATNGANN CTGNNNCCCN NNNNNNNNN //
Primer: BSD_R1 (Pr0758) Region: BSD 5',T2A,Venus 3' Sequence file: RDB12867_B2Abd.seq
>12867_12867_B2Ab_3_BSD_R1_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTGNAGA NGCGGCCGTC GCTAGAGAGA GCTGCNNCTG    61 GCGACGCTGT AGTCTTCAGA GATGGGGATG CTGTTGATTG TAGCCGTTGC TCTTTCAATG   121 AGGGTGGATT CTTCTTGAGA CAAAGGCTTG GCCATGGGCC CGGGATTTTC CTCCACGTCA   181 CCGCATGTTA GAAGACTTCC TCTGCCCTCG CCGGATCCCT TGTACAGCTC GTCCATGCCG   241 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG   301 GGGTCTTTGC TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG   361 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCTCG   421 ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA   481 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC   541 TTGAAGTCGA TGCCCTTCNG CTCGATGCGG TTCACCNGGG TGTCGCCCTC NAACTTCNCC   601 TCGGCGCGGG TCTTGNAGTT GCCNTCNNCC TTGAANAANN NGGTGCGCTC CTGGANNTAG   661 CCNTCNGGNN TGGCGGNNTT GNANAANTCN NGNTGNTNNN GNGGNNGGGN ANCNGGCNAN 721 NNNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB12867_B2Abe.seq
>12867_12867_B2Ab_3_CMV_Forward_A06_01_ABI08.ab1     1 NNNNNNNNNN NNNNCCNTTG NNGCNNNNGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG   121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT   181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT   241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA   301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG   361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG   421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC   481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG   541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA   601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA   661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG   721 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAN CACCGCACAG CAAGCGGCCG   781 CTGATCTTCA GACCTGGAGG ANGAGATATG ANGGACAATT GGAGAAGTGA ATTATATAAA   841 TATAAAGTAG TAAAAATTGA NCCNTTANGA GTAGCACCCA CCAAGGCAAA GAGAAGANTG   901 NTGCAGANAG AAAAAAGANC AGNGGGAATA NGANCTTTGT TCCTNGGGNT CTTGGGAGCA   961 NCANNNAGCA CTATGGNCGC AGCGNCNATG NNNNNNNNNG NACNNNCCNN NNNNNTANNG 1021 NCNNGNNNNN NNGNNNNNNN NNN //
Primer: RRE_F1 (Pr0796) Region: RRE,cPPT_CTS Sequence file: RDB12867_B2Abf.seq
>12867_12867_B2Ab_3_RRE_F1_B06_02_ABI08.ab1     1 NNNNNNNNNN NGNNNCNNCN NNNNNNTGCT GANGGCTATT GAGGCGCAAC AGCATCTGTT    61 GCAACTCACA GTCTGGGGCA TCAAGCAGCT CCAGGCAAGA ATCCTGGCTG TGGAAAGATA   121 CCTAAAGGAT CAACAGCTCC TGGGGATTTG GGGTTGCTCT GGAAAACTCA TTTGCACCAC   181 TGCTGTGCCT TGGAATGCTA GTTGGAGTAA TAAATCTCTG GAACAGATTT GGAATCACAC   241 GACCTGGATG GAGTGGGACA GAGAAATTAA CAATTACACA AGCTTAATAC ACTCCTTAAT   301 TGAAGAATCG CAAAACCAGC AAGAAAAGAA TGAACAAGAA TTATTGGAAT TAGATAAATG   361 GGCAAGTTTG TGGAATTGGT TTAACATAAC AAATTGGCTG TGGTATATAA AATTATTCAT   421 AATGATAGTA GGAGGCTTGG TAGGTTTAAG AATAGTTTTT GCTGTACTTT CTATAGTGAA   481 TAGAGTTAGG CAGGGATATT CACCATTATC GTTTCAGACC CACCTCCCAA CCCCGAGGGG   541 ACCCGACAGG CCCGAAGGAA TAGAAGAAGA AGGTGGAGAG AGAGACAGAG ACAGATCCAT   601 TCGATTAGTG AACGGATCTA CAAATGGCAG TATTCATCCA CAATTTTAAA AGAAAAGGGG   661 GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA   721 CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TCGGGTTTAT TACAGGGACA   781 GCAGAANTTC ACTTTGAATT ATCACAAGTT TGTACNAAAA AGCANGCTTT NAAGGAACCA   841 ATTCAGTCGA CTGGATCCGG TACCGAATTC GCGGCCGCAC TCGANANATC TAGANCCAGC   901 TTTCTTGNAC AAAGTGNTGA NAANTCNNGC TTCGTGAGGN TCCGGNGNCC GTCAGTGGGC   961 ANANCNCACA TCGCCCACAN NNCCCNANAN NNNNGGGGGN ANGGGNCGGN NNTTGAANCN 1021 NNNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: pGH pA,3' LTR Sequence file: RDB12867_B2Abg.seq
>12867_12867_B2Ab_3_bGH_rev3_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GGNNGGNNNN NNNACAGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA   121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG   181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC   241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC   301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC   361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC   421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA   481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA   541 GATCCNCAGA TCAAGGATAT CTTGTCTTCN TTGGGAGTGA ATTAGCCCTT CCNGTCCCCC   601 CTTTTCTTTT NAAAAGTGGC NNANATCTAC NGCTGNCNTG TAAGTCNTTG GTCTTNAAGG 661 NACNTGAGGN GNGANTGGAA AACCCNNCTC CNNNTCNNTG NGNTCNNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,Zeo,SV40 pA Sequence file: RDB12867_B2Abh.seq
>12867_12867_B2Ab_3_SV40pro_ori_F_D06_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCCTCTG CCTCTGANCT ATTCCNGAAG TAGTGAGGAG    61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG   121 GATCTGATCA GCACGTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA   181 CGACAAGGTG AGGAACTAAA CCATGGCCAA GTTGACCAGT GCCGTTCCGG TGCTCACCGC   241 GCGCGACGTC GCCGGAGCGG TCGAGTTCTG GACCGACCGG CTCGGGTTCT CCCGGGACTT   301 CGTGGAGGAC GACTTCGCCG GTGTGGTCCG GGACGACGTG ACCCTGTTCA TCAGCGCGGT   361 CCAGGACCAG GTGGTGCCGG ACAACACCCT GGCCTGGGTG TGGGTGCGCG GCCTGGACGA   421 GCTGTACGCC GAGTGGTCGG AGGTCGTGTC CACGAACTTC CGGGACGCCT CCGGGCCGGC   481 CATGACCGAG ATCGGCGAGC AGCCGTGGGG GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG   541 CAACTGCGTG CACTTCGTGG CCGAGGAGCA GGACTGACAC GTGCTACGAG ATTTCGATTC   601 CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT   661 GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC   721 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT   781 TTCACTGCAT TCTAGTTGTG GTTTGTCNNA ACTCATCNAT GTATCTTATC ATGTCTGTAT   841 ACCGTCGACC TCTANCTANA GCTTGNCGTA ATCATGGTCA TANCTGTTTC CTGTGTGAAA   901 NTGNTATCCG CTCACAANTC CNCACNACAT ACGAGCCGNA AGCATNAAGN GTAAAGNCTG   961 GGGTGNCNNN TGAGTGANCT ANCTCACNTT ANTTGCGTTG CNCTNNCNGC CCNCTTTNNN 1021 GNNNGNNNNN NNNCNNNNNN NCNGCNTNNN NNANNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl