Resource data sheet
DNA Bank Top

Histac-K12 pcDNA3.1 (#RDB12841)

For the detection of acetylation at the 12th lysine residues of histone H4 by the BRD2 bromodomain.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB12841.pdf
Publication Ito, T., Chem. Biol. 18 (4): 495-507 (2011). PMID 21513886. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. For the detection of acetylation at the 12th lysine residues of histone H4 by the BRD2 bromodomain.
Comment Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
Vector backbone pcDNA3.1 (Plasmid)
Size of vector backbone 5.5kb
Gene/insert name Human BRD2 cDNA Mouse Histone H4 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Sasaki, Kazuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Ito, T. et al., ChemBio. 18, 495-507, (2011)). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ito, T. et al., ChemBio. 18, 495-507, (2011))。本件リソースは学術研究機関のみに提供し、学術研究にのみ利用することができる。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog # Resource name Shipping form Fee
RDB12841 Histac-K12 pcDNA3.1 DNA solution

How to cite this biological resource

Materials & Methods section:

The Histac-K12 pcDNA3.1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12841).

Reference section:

Ito, T., Umehara, T., Sasaki, K., Nakamura, Y., Nishino, N., Terada, T., Shirouzu, M., Padmanabhan, B., Yokoyama, S., Ito, A., Yoshida, M., Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors. Chem. Biol. 18 (4): 495-507 (2011). PMID 21513886. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ito, T., Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors. Chem. Biol. 18 (4): 495-507 (2011). PMID 21513886. [PubMed] [Article] [RRC of NBRP]
reference He, M., Chemical Biology Approaches for Investigating the Functions of Lysine Acetyltransferases. Angew. Chem. Int. Ed. Engl. 57 (5): 1162-1184 (2018). PMID 28786225. [PubMed] [Article]
reference Soleja, N., Role of green fluorescent proteins and their variants in development of FRET-based sensors. J. Biosci. 43 (4): 763-784 (2018). PMID 30207321. [PubMed] [Article]