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Histac-K12 pcDNA3.1 (#RDB12841)

For the detection of acetylation at the 12th lysine residues of histone H4 by the BRD2 bromodomain.

Clone info. For the detection of acetylation at the 12th lysine residues of histone H4 by the BRD2 bromodomain.
Comment Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
Vector backbone pcDNA3.1 (Plasmid)
Size of vector backbone 5.5kb
Gene/insert name Human BRD2 cDNA Mouse Histone H4 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Sasaki, Kazuki |
 
Sequence RDB12841z.seq
Remarks, protocol and/or map (pdf) RDB12841.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Ito, T. et al., ChemBio. 18, 495-507, (2011)). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ito, T. et al., ChemBio. 18, 495-507, (2011))。本件リソースは学術研究機関のみに提供し、学術研究にのみ利用することができる。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12841 Histac-K12 pcDNA3.1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Histac-K12 pcDNA3.1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12841).

Reference section:

Ito, T., Umehara, T., Sasaki, K., Nakamura, Y., Nishino, N., Terada, T., Shirouzu, M., Padmanabhan, B., Yokoyama, S., Ito, A., Yoshida, M., Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors. Chem. Biol. 18 (4): 495-507 (2011). PMID 21513886. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12841_A4Lpp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB12841_A4Lpa.seq check
>12841_12841_A4Lp_CMV-Forward_D12_12.ab1
    1 CGTTACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CATGGTGAGC
  181 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  241 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  301 ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  361 ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC
  421 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  481 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  541 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  601 TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG
  661 GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC
  721 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  781 TACCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
  841 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGGA ATTCACCCCT
  901 GCCAACCCAC CACCCCCCGG AGGTGTCCAA TCCCAAAAAG CCAGGACGAG TTACCAACCA
  961 GCTGCAATAC CTACACAAGG TAGTGATGAA AGGCTCTGTG GAAACATCAG TTCGCATGGC
 1021 CCATTCCCGG CAGCCCTGTG GATGCTGTCA AACTGGGGTC TTTCCCGGGA TATCACAAAA
 1081 ATTATAAAAC AGGCCTATGA CATTGGTTAC TAATTAAGAG GAGACTTGAA AAACAACTAC
1141 TAACTGCGC
//
Primer: BGH_rev ()
Sequence file: RDB12841_A4Lpb.seq check
>12841_12841_A4Lp_BGHrev_E12_15.ab1
    1 GCCGACGGGG GCTCTAGACT CGAGTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC
   61 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT
  121 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT
  181 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG
  241 GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG
  301 GCTGATGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT
  361 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT
  421 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT
  481 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC
  541 GCCCCAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA
  601 GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT
  661 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA
  721 CAGCTCCTCG CCCTTGCTCA CCATGATATC AACCACTTTG TACAAGAAAG CTGGGTCGAC
  781 GCCGCCGAAG CCGTACAGAG TGCGGCCCTG GCGCTTGAGC GCGTACACCA CGTCCATAGC
  841 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC
  901 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC
  961 GCCGCCACGC CGAGCCAACC GCCGGATAGC GGGCTTGGTA ATGCCCTGAT GTTGTCGCGA
 1021 GAACCTTTGC GGTGTCGCTT GCGCCACCTT TCCCCAGACC CTTTGCCGCC TTTTCTCGTC
 1081 CTGACATGAT CAGATTCTTC ACACTGGATC ACACCTCAGG ATCTCGTCCA CCAGGAATCC
1141 AACCTTCT
//
Primer: SV40pro_F ()
Sequence file: RDB12841_A4Lpc.seq check
>12841_12841_A9Cr_SV40pro_F_A03_03_ABI24.ab1
    1 ACTCGTCCCT GGAACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
  961 CGCCCAACCT GCCATCACGA GGATTTCGAT TCCACCGCCG CCTTCTATGA AAGTTGGGCT
 1021 TCGGAATCGT TTCTGGACGC TGCCTGGATG ATTCTTCAGC GCGGGATCTC ATGCTGAGTC
1081 TGCCAACCCC AACTGTTAAT TGGCAAGCTT TATAAATTGG CTA
//
Primer: attB2-R1 ()
Sequence file: RDB12841_A4Lpd.seq check
>12841_12841_A9Cr_attB2-R1_B03_06_ABI24.ab1
    1 GCCCAAACGG AAAGAAGTGG CGGGCCCTGG GCGCTTGAGC GCGTACACCA CGTCCATAGC
   61 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC
  121 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC
  181 GCCGCCACGC CGAGCCAACC GCCGGATAGC GGGCTTGGTA ATGCCCTGGA TGTTGTCGCG
  241 GAGAACCTTG CGGTGTCGCT TGGCGCCACC TTTCCCCAGA CCCTTGCCGC CTTTTCCTCG
  301 TCCTGACATG GATCCAGATC CTCCACCACC TGAACCACCA CCTCCAGAAC CTCCTCCACC
  361 AGATCCACCT CCTCCGTCGA CGGCAGTAGA GACTGGTAAA GGCCCTGGTT CTAGTGGTTC
  421 ATCTGTCTTC TTGGCATAAC GGAACTCAAA TACATCCTGT AGCTTTCGTG CCATTGCCAC
  481 AACATCGTGA TCTGGGGGAT TGTACTTATA GCAGTTGGAG AACATAAGCC GTACATCAGC
  541 AGCAAACTCC TGTGCATCCC GGTAATCACG GTTCTCCATC TTCCGCTTGA CAGTGCTGAG
  601 GTCCATGGGG TGCTTAATGA TGTCATGGTA GTCATGCAGG CCAAGTGCAG AAGCATCCAC
  661 TGGTTTATAG AAAGGCCAAG CATAGGCAGC ATGCTTCTTA GAAGAGTAAC TCCTTCAAAT
  721 GCCATTGGCA AATGTTTTAA CTGTTCTGAA AGCTTTCCTT TCTTAGAGGC TCTGGTGTTG
781 TTGCTGAGAG TCAGGCAAGT CTTTTCGTGG GGGCTTGATG GGGCGAACCA ACTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ito, T., Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors. Chem. Biol. 18 (4): 495-507 (2011). PMID 21513886. [link to RRC of NBRP]
reference He, M., Chemical Biology Approaches for Investigating the Functions of Lysine Acetyltransferases. Angew. Chem. Int. Ed. Engl. 57 (5): 1162-1184 (2018). PMID 28786225.
reference Soleja, N., Role of green fluorescent proteins and their variants in development of FRET-based sensors. J. Biosci. 43 (4): 763-784 (2018). PMID 30207321.