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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Histac-K12 pcDNA3.1

For the detection of acetylation at the 12th lysine residues of histone H4 by the BRD2 bromodomain.

Catalog number RDB12841
Resource name Histac-K12 pcDNA3.1
Clone info. For the detection of acetylation at the 12th lysine residues of histone H4 by the BRD2 bromodomain.
Comment Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
Vector backbone pcDNA3.1 (Plasmid)
Size of vector backbone 5.5kb
Gene/insert name Human BRD2 cDNA Mouse Histone H4 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Sasaki, Kazuki |
Other clones in our bank

External Database
Human BRD2 Mouse Histone H4 Aequorea victoria GFP

        Reference sequence
          
         
        Sequence RDB12841z.seq
        Remarks, protocol and/or map (pdf) RDB12841.pdf

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Ito, T. et al., ChemBio. 18, 495-507, (2011)). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
        Additional terms and conditions:
        RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ito, T. et al., ChemBio. 18, 495-507, (2011))。本件リソースは学術研究機関のみに提供し、学術研究にのみ利用することができる。
        付加的提供条件:
        利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
        宮脇敦史博士による提供承諾書 [Word]
        備考
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB12841 Histac-K12 pcDNA3.1 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Ito, T., Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors. Chem. Biol. 18 (4): 495-507 (2011). PMID 21513886.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Epigenetics clone (English text)
        Featured content Histac for visualization of acetylation of the Histon (English text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB12841_A4Lpp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV-Forward
        Sequence file: RDB12841_A4Lpa.seq check
        >12841_12841_A4Lp_CMV-Forward_D12_12.ab1
            1 CGTTACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
           61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
          121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CATGGTGAGC
          181 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
          241 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
          301 ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
          361 ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC
          421 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
          481 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
          541 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
          601 TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG
          661 GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC
          721 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
          781 TACCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
          841 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGGA ATTCACCCCT
          901 GCCAACCCAC CACCCCCCGG AGGTGTCCAA TCCCAAAAAG CCAGGACGAG TTACCAACCA
          961 GCTGCAATAC CTACACAAGG TAGTGATGAA AGGCTCTGTG GAAACATCAG TTCGCATGGC
         1021 CCATTCCCGG CAGCCCTGTG GATGCTGTCA AACTGGGGTC TTTCCCGGGA TATCACAAAA
         1081 ATTATAAAAC AGGCCTATGA CATTGGTTAC TAATTAAGAG GAGACTTGAA AAACAACTAC
        1141 TAACTGCGC
        //
        Primer: BGH_rev
        Sequence file: RDB12841_A4Lpb.seq check
        >12841_12841_A4Lp_BGHrev_E12_15.ab1
            1 GCCGACGGGG GCTCTAGACT CGAGTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC
           61 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT
          121 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT
          181 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG
          241 GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG
          301 GCTGATGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT
          361 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT
          421 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT
          481 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC
          541 GCCCCAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA
          601 GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT
          661 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA
          721 CAGCTCCTCG CCCTTGCTCA CCATGATATC AACCACTTTG TACAAGAAAG CTGGGTCGAC
          781 GCCGCCGAAG CCGTACAGAG TGCGGCCCTG GCGCTTGAGC GCGTACACCA CGTCCATAGC
          841 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC
          901 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC
          961 GCCGCCACGC CGAGCCAACC GCCGGATAGC GGGCTTGGTA ATGCCCTGAT GTTGTCGCGA
         1021 GAACCTTTGC GGTGTCGCTT GCGCCACCTT TCCCCAGACC CTTTGCCGCC TTTTCTCGTC
         1081 CTGACATGAT CAGATTCTTC ACACTGGATC ACACCTCAGG ATCTCGTCCA CCAGGAATCC
        1141 AACCTTCT
        //
        Primer: SV40pro_F
        Sequence file: RDB12841_A4Lpc.seq check
        >12841_12841_A9Cr_SV40pro_F_A03_03_ABI24.ab1
            1 ACTCGTCCCT GGAACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
           61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
          121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
          181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
          241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
          301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
          361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
          421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
          481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
          541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
          601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
          661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
          721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
          781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
          841 ACCGCTTCCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
          901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
          961 CGCCCAACCT GCCATCACGA GGATTTCGAT TCCACCGCCG CCTTCTATGA AAGTTGGGCT
         1021 TCGGAATCGT TTCTGGACGC TGCCTGGATG ATTCTTCAGC GCGGGATCTC ATGCTGAGTC
        1081 TGCCAACCCC AACTGTTAAT TGGCAAGCTT TATAAATTGG CTA
        //
        Primer: attB2-R1
        Sequence file: RDB12841_A4Lpd.seq check
        >12841_12841_A9Cr_attB2-R1_B03_06_ABI24.ab1
            1 GCCCAAACGG AAAGAAGTGG CGGGCCCTGG GCGCTTGAGC GCGTACACCA CGTCCATAGC
           61 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC
          121 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC
          181 GCCGCCACGC CGAGCCAACC GCCGGATAGC GGGCTTGGTA ATGCCCTGGA TGTTGTCGCG
          241 GAGAACCTTG CGGTGTCGCT TGGCGCCACC TTTCCCCAGA CCCTTGCCGC CTTTTCCTCG
          301 TCCTGACATG GATCCAGATC CTCCACCACC TGAACCACCA CCTCCAGAAC CTCCTCCACC
          361 AGATCCACCT CCTCCGTCGA CGGCAGTAGA GACTGGTAAA GGCCCTGGTT CTAGTGGTTC
          421 ATCTGTCTTC TTGGCATAAC GGAACTCAAA TACATCCTGT AGCTTTCGTG CCATTGCCAC
          481 AACATCGTGA TCTGGGGGAT TGTACTTATA GCAGTTGGAG AACATAAGCC GTACATCAGC
          541 AGCAAACTCC TGTGCATCCC GGTAATCACG GTTCTCCATC TTCCGCTTGA CAGTGCTGAG
          601 GTCCATGGGG TGCTTAATGA TGTCATGGTA GTCATGCAGG CCAAGTGCAG AAGCATCCAC
          661 TGGTTTATAG AAAGGCCAAG CATAGGCAGC ATGCTTCTTA GAAGAGTAAC TCCTTCAAAT
          721 GCCATTGGCA AATGTTTTAA CTGTTCTGAA AGCTTTCCTT TCTTAGAGGC TCTGGTGTTG
        781 TTGCTGAGAG TCAGGCAAGT CTTTTCGTGG GGGCTTGATG GGGCGAACCA ACTC
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Ito, T., Real-time imaging of histone H4K12-specific acetylation determines the modes of action of histone deacetylase and bromodomain inhibitors. Chem. Biol. 18 (4): 495-507 (2011). PMID 21513886.
        reference He, M., Chemical Biology Approaches for Investigating the Functions of Lysine Acetyltransferases. Angew. Chem. Int. Ed. Engl. 57 (5): 1162-1184 (2018). PMID 28786225.
        reference Soleja, N., Role of green fluorescent proteins and their variants in development of FRET-based sensors. J. Biosci. 43 (4): 763-784 (2018). PMID 30207321.

        2022.10.12

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