Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGFP-Phc2 L307R

Probe for PcG complex. Expression clone of mouse Phc2 fused with GFP

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Developmental Cell, 26, 565-577(2013)).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Developmental Cell, 26, 565-577(2013))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12788_A4Ekp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C (Pr0025) Region: EGFP, insert Sequence file: RDB12788_A4Eka.seq
>12788_EGFP_C_E10_13.ab1     1 AGGTTCTCTC GGCATGGACG AGCTGTACAA GTCCGGCCGG ACTCAGATCT CGAGCTCAAG    61 CTTCGAATTC ATGACCTCAG GGAACGGAAA CTCTGCCTCC AGCATCGCCG GCACTGCCCC   121 CCAGAATGGT GAGAATAAAC CACCACAGGC CATTGTGAAA CCCCAAATCC TGACGCATGT   181 TATCGAAGGG TTTGTGATCC AGGAGGGGGC GGAGCCTTTC CCGGTGGGAC GCTCGTCCCT   241 GCTGGTAGGG AATCTCAAAA AGAAGTATGC ACAGGGGTTC TTGCCTGAGA AGCCTCCACA   301 GCAGGACCAC ACCACCACCA CCGACTCAGA GATGGAAGAG CCCTACCTGC AAGAATCCAA   361 AGAGGAGGGC ACTCCCCTCA AACTCAAGTG TGAGCTCTGT GGACGGGTGG ACTTTGCCTA   421 CAAGTTCAAG CGTTCCAAGC GCTTCTGTTC CATGGCTTGT GCAAAGAGGT ATAATGTGGG   481 ATGCACCAAA CGAGTGGGAC TTTTTCACTC AGACCGAAGC AAGCTGCAGA AGGCAGGGAC   541 CACAACCCAC AACCGCCGAC GGGCCAGCAA GGCCAGTCTG CCCACACTCA CCAAGGACAC   601 CAAGAAGCAG CCCTCAGGCA CTGTACCCCT TTCAGTTACT GCCGCCTTGC AGCTGGCGCA   661 CAGCCAGGAG GACTCCAGCC GGTGCTCAGA TAACTCCAGC TATGAGGAGC CCTTGTCACC   721 CATCTCAGCC AGCTCGTCCA CCTCGCGACG GCGCCAAGGC CAGAGGGACC TGGATCTCCC   781 TGACATGCAC ATGAGGGACC TTGTGGGCGT GGGACACCAC TTCCTGCCGA GCGAGCCCAC   841 AAAATGGAAT GTAGAGGATG TCTATGAGTT CATCCGCTCT CTGCCAGGCT GCCAGGAGAT   901 CGCGGAGGAG TTCCGTGCGC AGGAGATTGA TGGGCAAGCC TTGCTGCTGC TCAAGGAGGA   961 CCACCTAATG AGCGCCATGA ACATCAAGCG AGGGCCCGCC CTGAAGATCT ACGCACGCAT  1021 CAGCATGCTC AAGGACTCCT AGAGTCTCTG CTGCCTGCCC ACTGCACACC AGGCAGCCAG  1081 GATTCTAACC CCATGCAGCA CGCCTCCGGA TGGAAGCGAG CCTCACGGAC ATTCTGAGAG 1141 CCAGGAAAGG TCCAGCCTTG GGGCGGGAAG GGGC //
Primer: ME-1290RV (Pr0046) Region: insert Sequence file: RDB12788_A4Ekb.seq
>12788_ME_1290Rv_F10_16.ab1     1 ATTGCAATTT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA    61 GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAG TTATCTAGAT CCGGTGGATC   121 CCGGGCCCGC GGTACCGTCG ACTGCAGAAT TCGCGGCCGC GTCGACGCCG GTGCTATCAG   181 TATTTACAAG CGCAAAGCCC CATCTTCTGT GCCACCTACC TCTACCCCTT CCACAGAAAT   241 GGCCCCTGAG AGCCACTGAA GAGGTACTCG GACCTCCTCC CAGCTGCACC CCTAGGGAGA   301 GCCCCTTCCC GCCAAGCTGG CCCTTTCCTG GGCTCCTCAG GAATGTCCGT GAGGCTCTGC   361 TCCATCCGGA GGCGGTTGCC TGCATGGGGT TAGAATCCTG GCTGCCTGGT GGTGCAGTGG   421 GCAGGCAGCA GAGACTCTAG GAGTCCTTGA GCATGCTGAT GCGTGCGTAG ATCTTCAGGG   481 CGGGCCCTCG CTTGATGTTC ATGGCGCTCA TTAGGTGGTC CTCCTTGAGC AGCAGCAAGG   541 CTTGCCCATC AATCTCCTGC GCACGGAACT CCTCCGCGAT CTCCTGGCAG CCTGGCAGAG   601 AGCGGATGAA CTCATAGACA TCCTCTACAT TCCATTTTGT GGGCTCGCTC GGCAGGAAGT   661 GGTGTCCCAC GCCCACAAGG TCCCTCATGT GCATGTCAGG GAGATCCAGG TCCCTCTGGC   721 CTTGGCGCCG TCGCGAGGTG GACGAGCTGG CTGAGATGGG TGACAAGGGC TCCTCATAGC   781 TGGAGTTATC TGAGCACCGG CTGGAGTCCT CCTGGCTGTG CGCCAGCTGC AAGGCGGCAG   841 TAACTGAAAG GGGTACAGTG CCTGAGGGCT GCTTCTTGGT GTCCTTGGTG AGTGTGGGCA   901 GACTGGCCTT GCTGGCCCGT CGGCGGTTGT GGGTTGTGGT CCCTGCCTTC TGCAGCTTGC   961 TTCGGTCTGA GTGAAAAAGT CCCACTCGTT TGGTGCATCC CACATTATAC CTCTTTTGCA  1021 CAGCCCATGA ACAGAGCGCT TGGAACGCTT GAACTTGTAG GCAAAGTTCC ACCCGTCACA  1081 GAGCTCACAC TTGAGTTGAG GGGATTGCTT CCTCTTGATC AGCAGTAGGC TCTTCCAATC 1141 TCTGGAAGTC GTGAT //
Primer: CMV_Forward (Pr0016) Region: CMV pro, EGFP, insert 5' Sequence file: RDB12788_A4Ekc.seq
>12788_CMV_Forward_D10_10.ab1     1 CGTGTCCCCA CTCCCGCCCA ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA   121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG   181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG   241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC   301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC   361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT   421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG   481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA   541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG   601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG   661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT   721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC   781 TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTCCG   841 GCCGGACTCA GATCTCGAGC TCAAGCTTCG AATTCATGAC CTCAGGGAAC GGAAACTCTG   901 CCTCCAGCAT CGCCGGGCAC TGCCCCCCAG AATGGTGAGA ATAAACCACC ACAGGCCATT   961 GTGAAACCCA AATCCTTGAC GCATGTTATC GAAAGGGTTT GTGATCCAGG GAGGGGGCGG  1021 AGCCCTTTCC CGGTGGGGAC GCTCGTTCCC TGCCTGGGTA GGGAATCTCA AAAGAGTATG  1081 CACCAGGGGT TCCTTGCCTG AAGAAGTCTT CCACAGGCAT GAACCACTAC TACATCAACG 1141 AACTCAGAGG AATGGAAAAG CCCCTTACCT GTGCAAGGAA T //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB12788_A4Ekd.seq
>12788_12788_B0FnF_SV40pro_ori_F_B12_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTCGNCCTC TGAGCNATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA   121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG   181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG   241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC   301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC   361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG   421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC   481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA   541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG   601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG   661 GCGANGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG   721 GCCGCTTTTC TGGATTCNTC GACNGTGGCC GGCTGNNNGT GGCGGANCGC TATCANGNNN   781 TANCGTTGGC TANCCNNGAT ATTGNTGNNN ANCTTNNNNG CGNANGGNNN GANNGCNTNN 841 NCNNNNNTNN NNNNATCNNN GCTNCNNANN NNNNNCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl