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pGFP-Phc2 L307R (#RDB12788)

Probe for PcG complex. Expression clone of mouse Phc2 fused with GFP

Clone info. Expression clone of mouse Phc2 L307R mutant fused with GFP.
Comment Probe for PcG complex.
Vector backbone pEGFP-C2 (Plasmid)
Selectable markers Kan^r
Gene/insert name Mouse Phc2 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Koseki, Haruhiko | Isono, Kyoichi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Developmental Cell, 26, 565-577(2013)).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Developmental Cell, 26, 565-577(2013))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12788 pGFP-Phc2 L307R DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGFP-Phc2 L307R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12788).

Reference section:

Isono, K., Endo, T.A., Ku, M., Yamada, D., Suzuki, R., Sharif, J., Ishikura, T., Toyoda, T., Bernstein, B.E., Koseki, H., SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Dev. Cell 26 (6): 565-577 (2013). PMID 24091011. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12788_A4Ekp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C (Pr0025)
Region: EGFP, insert
Sequence file: RDB12788_A4Eka.seq check
>12788_EGFP_C_E10_13.ab1
    1 AGGTTCTCTC GGCATGGACG AGCTGTACAA GTCCGGCCGG ACTCAGATCT CGAGCTCAAG
   61 CTTCGAATTC ATGACCTCAG GGAACGGAAA CTCTGCCTCC AGCATCGCCG GCACTGCCCC
  121 CCAGAATGGT GAGAATAAAC CACCACAGGC CATTGTGAAA CCCCAAATCC TGACGCATGT
  181 TATCGAAGGG TTTGTGATCC AGGAGGGGGC GGAGCCTTTC CCGGTGGGAC GCTCGTCCCT
  241 GCTGGTAGGG AATCTCAAAA AGAAGTATGC ACAGGGGTTC TTGCCTGAGA AGCCTCCACA
  301 GCAGGACCAC ACCACCACCA CCGACTCAGA GATGGAAGAG CCCTACCTGC AAGAATCCAA
  361 AGAGGAGGGC ACTCCCCTCA AACTCAAGTG TGAGCTCTGT GGACGGGTGG ACTTTGCCTA
  421 CAAGTTCAAG CGTTCCAAGC GCTTCTGTTC CATGGCTTGT GCAAAGAGGT ATAATGTGGG
  481 ATGCACCAAA CGAGTGGGAC TTTTTCACTC AGACCGAAGC AAGCTGCAGA AGGCAGGGAC
  541 CACAACCCAC AACCGCCGAC GGGCCAGCAA GGCCAGTCTG CCCACACTCA CCAAGGACAC
  601 CAAGAAGCAG CCCTCAGGCA CTGTACCCCT TTCAGTTACT GCCGCCTTGC AGCTGGCGCA
  661 CAGCCAGGAG GACTCCAGCC GGTGCTCAGA TAACTCCAGC TATGAGGAGC CCTTGTCACC
  721 CATCTCAGCC AGCTCGTCCA CCTCGCGACG GCGCCAAGGC CAGAGGGACC TGGATCTCCC
  781 TGACATGCAC ATGAGGGACC TTGTGGGCGT GGGACACCAC TTCCTGCCGA GCGAGCCCAC
  841 AAAATGGAAT GTAGAGGATG TCTATGAGTT CATCCGCTCT CTGCCAGGCT GCCAGGAGAT
  901 CGCGGAGGAG TTCCGTGCGC AGGAGATTGA TGGGCAAGCC TTGCTGCTGC TCAAGGAGGA
  961 CCACCTAATG AGCGCCATGA ACATCAAGCG AGGGCCCGCC CTGAAGATCT ACGCACGCAT
 1021 CAGCATGCTC AAGGACTCCT AGAGTCTCTG CTGCCTGCCC ACTGCACACC AGGCAGCCAG
 1081 GATTCTAACC CCATGCAGCA CGCCTCCGGA TGGAAGCGAG CCTCACGGAC ATTCTGAGAG
1141 CCAGGAAAGG TCCAGCCTTG GGGCGGGAAG GGGC
//
Primer: ME-1290RV (Pr0046)
Region: insert
Sequence file: RDB12788_A4Ekb.seq check
>12788_ME_1290Rv_F10_16.ab1
    1 ATTGCAATTT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA
   61 GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAG TTATCTAGAT CCGGTGGATC
  121 CCGGGCCCGC GGTACCGTCG ACTGCAGAAT TCGCGGCCGC GTCGACGCCG GTGCTATCAG
  181 TATTTACAAG CGCAAAGCCC CATCTTCTGT GCCACCTACC TCTACCCCTT CCACAGAAAT
  241 GGCCCCTGAG AGCCACTGAA GAGGTACTCG GACCTCCTCC CAGCTGCACC CCTAGGGAGA
  301 GCCCCTTCCC GCCAAGCTGG CCCTTTCCTG GGCTCCTCAG GAATGTCCGT GAGGCTCTGC
  361 TCCATCCGGA GGCGGTTGCC TGCATGGGGT TAGAATCCTG GCTGCCTGGT GGTGCAGTGG
  421 GCAGGCAGCA GAGACTCTAG GAGTCCTTGA GCATGCTGAT GCGTGCGTAG ATCTTCAGGG
  481 CGGGCCCTCG CTTGATGTTC ATGGCGCTCA TTAGGTGGTC CTCCTTGAGC AGCAGCAAGG
  541 CTTGCCCATC AATCTCCTGC GCACGGAACT CCTCCGCGAT CTCCTGGCAG CCTGGCAGAG
  601 AGCGGATGAA CTCATAGACA TCCTCTACAT TCCATTTTGT GGGCTCGCTC GGCAGGAAGT
  661 GGTGTCCCAC GCCCACAAGG TCCCTCATGT GCATGTCAGG GAGATCCAGG TCCCTCTGGC
  721 CTTGGCGCCG TCGCGAGGTG GACGAGCTGG CTGAGATGGG TGACAAGGGC TCCTCATAGC
  781 TGGAGTTATC TGAGCACCGG CTGGAGTCCT CCTGGCTGTG CGCCAGCTGC AAGGCGGCAG
  841 TAACTGAAAG GGGTACAGTG CCTGAGGGCT GCTTCTTGGT GTCCTTGGTG AGTGTGGGCA
  901 GACTGGCCTT GCTGGCCCGT CGGCGGTTGT GGGTTGTGGT CCCTGCCTTC TGCAGCTTGC
  961 TTCGGTCTGA GTGAAAAAGT CCCACTCGTT TGGTGCATCC CACATTATAC CTCTTTTGCA
 1021 CAGCCCATGA ACAGAGCGCT TGGAACGCTT GAACTTGTAG GCAAAGTTCC ACCCGTCACA
 1081 GAGCTCACAC TTGAGTTGAG GGGATTGCTT CCTCTTGATC AGCAGTAGGC TCTTCCAATC
1141 TCTGGAAGTC GTGAT
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP, insert 5'
Sequence file: RDB12788_A4Ekc.seq check
>12788_CMV_Forward_D10_10.ab1
    1 CGTGTCCCCA CTCCCGCCCA ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
  121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
  241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
  301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
  361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
  421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
  481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
  541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
  601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
  661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
  721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
  781 TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTCCG
  841 GCCGGACTCA GATCTCGAGC TCAAGCTTCG AATTCATGAC CTCAGGGAAC GGAAACTCTG
  901 CCTCCAGCAT CGCCGGGCAC TGCCCCCCAG AATGGTGAGA ATAAACCACC ACAGGCCATT
  961 GTGAAACCCA AATCCTTGAC GCATGTTATC GAAAGGGTTT GTGATCCAGG GAGGGGGCGG
 1021 AGCCCTTTCC CGGTGGGGAC GCTCGTTCCC TGCCTGGGTA GGGAATCTCA AAAGAGTATG
 1081 CACCAGGGGT TCCTTGCCTG AAGAAGTCTT CCACAGGCAT GAACCACTAC TACATCAACG
1141 AACTCAGAGG AATGGAAAAG CCCCTTACCT GTGCAAGGAA T
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB12788_A4Ekd.seq check
>12788_12788_B0FnF_SV40pro_ori_F_B12_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCGNCCTC TGAGCNATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
  121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
  181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
  241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
  301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
  361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
  421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
  481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
  541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG
  601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG
  661 GCGANGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
  721 GCCGCTTTTC TGGATTCNTC GACNGTGGCC GGCTGNNNGT GGCGGANCGC TATCANGNNN
  781 TANCGTTGGC TANCCNNGAT ATTGNTGNNN ANCTTNNNNG CGNANGGNNN GANNGCNTNN
841 NCNNNNNTNN NNNNATCNNN GCTNCNNANN NNNNNCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Isono, K., SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Dev. Cell 26 (6): 565-577 (2013). PMID 24091011. [link to RRC of NBRP]