Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGFP-Phc2

Probe for PcG complex. Expression clone of mouse Phc2 fused with GFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Developmental Cell, 26, 565-577(2013)).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Developmental Cell, 26, 565-577(2013))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12787_A4Ekp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C (Pr0025) Region: EGFP, insert 5' Sequence file: RDB12787_A4Eka.seq
>12787_EGFP_C_B10_04.ab1     1 AGGGGATATT CTCGGGGCAT GGGACGAGCT GTACAAGTCC GGCCGGACTC AGATCTCGAG    61 CTCAAGCTTC GAATTCATGA CCTCAGGGAA CGGAAACTCT GCCTCCAGCA TCGCCGGCAC   121 TGCCCCCCAG AATGGTGAGA ATAAACCACC ACAGGCCATT GTGAAACCCC AAATCCTGAC   181 GCATGTTATC GAAGGGTTTG TGATCCAGGA GGGGGCGGAG CCTTTCCCGG TGGGACGCTC   241 GTCCCTGCTG GTAGGGAATC TCAAAAAGAA GTATGCACAG GGGTTCTTGC CTGAGAAGCC   301 TCCACAGCAG GACCACACCA CCACCACCGA CTCAGAGATG GAAGAGCCCT ACCTGCAAGA   361 ATCCAAAGAG GAGGGCACTC CCCTCAAACT CAAGTGTGAG CTCTGTGGAC GGGTGGACTT   421 TGCCTACAAG TTCAAGCGTT CCAAGCGCTT CTGTTCCATG GCTTGTGCAA AGAGGTATAA   481 TGTGGGATGC ACCAAACGAG TGGGACTTTT TCACTCAGAC CGAAGCAAGC TGCAGAAGGC   541 AGGGACCACA ACCCACAACC GCCGACGGGC CAGCAAGGCC AGTCTGCCCA CACTCACCAA   601 GGACACCAAG AAGCAGCCCT CAGGCACTGT ACCCCTTTCA GTTACTGCCG CCTTGCAGCT   661 GGCGCACAGC CAGGAGGACT CCAGCCGGTG CTCAGATAAC TCCAGCTATG AGGAGCCCTT   721 GTCACCCATC TCAGCCAGCT CGTCCACCTC GCGACGGCGC CAAGGCCAGA GGGACCTGGA   781 TCTCCCTGAC ATGCACATGA GGGACCTTGT GGGCGTGGGA CACCACTTCC TGCCGAGCGA   841 GCCCACAAAA TGGAATGTAG AGGATGTCTA TGAGTTCATC CGCTCTCTGC CAGGCTGCCA   901 GGAGATCGCG GAGGGAGTTC CGTGCGCAGG AGATTGATGG GCAAGCCTTG CTGCTGCTCA   961 AGGGAGGACC ACCTAAATGA GCGCCATGAA CATCAAGCTA GGGCCCGCCC CTGAAAGATC  1021 TACGCACGCA TCAGCATGCT CAAGGACTCC TAGAGTCTCT GCTGCCTGCT CACTGCACCA  1081 CCAGGCAGCA GGATTCTAAC CCATGCAGGC ACGCTCGGGA TGGAAGCAGA GCTTCACGAC 1141 ATCTGAGGAG CCCAGGAAGG CCAGCCTTGG GCGGGAAAGG //
Primer: ME-1290RV (Pr0046) Region: insert 3' Sequence file: RDB12787_A4Ekb.seq
>12787_ME_1290Rv_C10_07.ab1     1 ATGGGCATTT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA    61 GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAG TTATCTAGAT CCGGTGGATC   121 CCGGGCCCGC GGTACCGTCG ACTGCAGAAT TCGCGGCCGC GTCGACGCCG GTGCTATCAG   181 TATTTACAAG CGCAAAGCCC CATCTTCTGT GCCACCTACC TCTACCCCTT CCACAGAAAT   241 GGCCCCTGAG AGCCACTGAA GAGGTACTCG GACCTCCTCC CAGCTGCACC CCTAGGGAGA   301 GCCCCTTCCC GCCAAGCTGG CCCTTTCCTG GGCTCCTCAG GAATGTCCGT GAGGCTCTGC   361 TCCATCCGGA GGCGGTTGCC TGCATGGGGT TAGAATCCTG GCTGCCTGGT GGTGCAGTGG   421 GCAGGCAGCA GAGACTCTAG GAGTCCTTGA GCATGCTGAT GCGTGCGTAG ATCTTCAGGG   481 CGGGCCCTAG CTTGATGTTC ATGGCGCTCA TTAGGTGGTC CTCCTTGAGC AGCAGCAAGG   541 CTTGCCCATC AATCTCCTGC GCACGGAACT CCTCCGCGAT CTCCTGGCAG CCTGGCAGAG   601 AGCGGATGAA CTCATAGACA TCCTCTACAT TCCATTTTGT GGGCTCGCTC GGCAGGAAGT   661 GGTGTCCCAC GCCCACAAGG TCCCTCATGT GCATGTCAGG GAGATCCAGG TCCCTCTGGC   721 CTTGGCGCCG TCGCGAGGTG GACGAGCTGG CTGAGATGGG TGACAAGGGC TCCTCATAGC   781 TGGAGTTATC TGAGCACCGG CTGGAGTCCT CCTGGCTGTG CGCCAGCTGC AAGGCGGCAG   841 TAACTGAAAG GGGTACAGTG CCTGAGGGCT GCTTCTTGGT GTCCTTGGTG AGTGTGGGCA   901 GACTGGCCTT GCTGGCCCGT CGGCGGTTGT GGGTTGTGGT CCCTGCCTTC TGCAGCTTGC   961 TTCGGTCTGA GTGAAAAAAG TCCCACTCGT TTGGTGCATC CCACATTATA CCTCTTTGCA  1021 CAAGCCAATG GAACAGAAGC GCTTGGAACG CTTGAACTTG TAGCAAGTCC ACCCGTTCAC  1081 AGAGCTCACA CTTGAGTTGA GGGGAGTGCC TCCTCTTGGA TTCTTGCAGT AAGGCTCTCC 1141 AATCCTCTGA AGTCCGTGAT //
Primer: CMV_Forward (Pr0016) Region: CMV pro, EGFP, insert 5' Sequence file: RDB12787_A4Ekc.seq
>12787_CMV_Forward_A10_01.ab1     1 TTGATCCACT TCCGCCCCAA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT    61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT   121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG   181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG   241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT   301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA   361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT   421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT   481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA   541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG   601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA   661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA   721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT   781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG   841 CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCATGACC TCAGGGAACG GAAACTCTGC   901 CTCCAGCATC GCCGGCACTG CCCCCCAGAA TGGTGAGAAT AAACCACCAC AGGCCATTGT   961 GAAACCCCAA ATCCTGACGC ATGTTATCGA AAGGTTTTGT GATCCAGGAG GGGGCGGGAG  1021 CCCTTTCCCG GTGGGACGCT CGTCTCTGCT GGTTAGGAAT CTCAAAAAGA AAGTATGCAC  1081 AGGGGTTTCT GGCCTGAGAA GCCTCCACAG CAGACACACA ACATCAACCG AACTCAGAGA 1141 TTGGAGAAGC CCTAACTGGC CAAGAAAAAA TC //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB12787_A4Ekd.seq
>12787_12787_B0FnF_SV40pro_ori_F_A12_03_ABI24.ab1     1 NNNNNNNNNN NGCCGCCNCN GCCTCTGANC TATTCNNGAA GTAGTGAGGA GGCTTTTTTG    61 GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT   121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT   181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG   241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC   301 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC   361 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC   421 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG   481 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG   541 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT   601 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAN   661 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC   721 TTTTCTGGAT TCNTCGACNG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA NGACATAGCG   781 TTGGCTACNC GNNATATTGC TGAAGANCTN GNCGGCGAAN GGGCTGANCG CTTCCTCNNG   841 CTTTACNGTA TNGCCGCTCC CNANTCNCAG CGCATCGCCT TCNNNCGCCN TNTNNNNNAN 901 NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl