Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGFP-Phc2 (#RDB12787)

Probe for PcG complex. Expression clone of mouse Phc2 fused with GFP.

Clone info. Expression clone of mouse Phc2 fused with GFP.
Comment Probe for PcG complex.
Vector backbone pEGFP-C2 (Plasmid)
Selectable markers Kan^r
Gene/insert name Mouse Phc2 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Koseki, Haruhiko | Isono, Kyoichi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Developmental Cell, 26, 565-577(2013)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Developmental Cell, 26, 565-577(2013))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12787 pGFP-Phc2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGFP-Phc2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12787).

Reference section:

Isono, K., Endo, T.A., Ku, M., Yamada, D., Suzuki, R., Sharif, J., Ishikura, T., Toyoda, T., Bernstein, B.E., Koseki, H., SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Dev. Cell 26 (6): 565-577 (2013). PMID 24091011. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12787_A4Ekp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C (Pr0025)
Region: EGFP, insert 5'
Sequence file: RDB12787_A4Eka.seq check
>12787_EGFP_C_B10_04.ab1
    1 AGGGGATATT CTCGGGGCAT GGGACGAGCT GTACAAGTCC GGCCGGACTC AGATCTCGAG
   61 CTCAAGCTTC GAATTCATGA CCTCAGGGAA CGGAAACTCT GCCTCCAGCA TCGCCGGCAC
  121 TGCCCCCCAG AATGGTGAGA ATAAACCACC ACAGGCCATT GTGAAACCCC AAATCCTGAC
  181 GCATGTTATC GAAGGGTTTG TGATCCAGGA GGGGGCGGAG CCTTTCCCGG TGGGACGCTC
  241 GTCCCTGCTG GTAGGGAATC TCAAAAAGAA GTATGCACAG GGGTTCTTGC CTGAGAAGCC
  301 TCCACAGCAG GACCACACCA CCACCACCGA CTCAGAGATG GAAGAGCCCT ACCTGCAAGA
  361 ATCCAAAGAG GAGGGCACTC CCCTCAAACT CAAGTGTGAG CTCTGTGGAC GGGTGGACTT
  421 TGCCTACAAG TTCAAGCGTT CCAAGCGCTT CTGTTCCATG GCTTGTGCAA AGAGGTATAA
  481 TGTGGGATGC ACCAAACGAG TGGGACTTTT TCACTCAGAC CGAAGCAAGC TGCAGAAGGC
  541 AGGGACCACA ACCCACAACC GCCGACGGGC CAGCAAGGCC AGTCTGCCCA CACTCACCAA
  601 GGACACCAAG AAGCAGCCCT CAGGCACTGT ACCCCTTTCA GTTACTGCCG CCTTGCAGCT
  661 GGCGCACAGC CAGGAGGACT CCAGCCGGTG CTCAGATAAC TCCAGCTATG AGGAGCCCTT
  721 GTCACCCATC TCAGCCAGCT CGTCCACCTC GCGACGGCGC CAAGGCCAGA GGGACCTGGA
  781 TCTCCCTGAC ATGCACATGA GGGACCTTGT GGGCGTGGGA CACCACTTCC TGCCGAGCGA
  841 GCCCACAAAA TGGAATGTAG AGGATGTCTA TGAGTTCATC CGCTCTCTGC CAGGCTGCCA
  901 GGAGATCGCG GAGGGAGTTC CGTGCGCAGG AGATTGATGG GCAAGCCTTG CTGCTGCTCA
  961 AGGGAGGACC ACCTAAATGA GCGCCATGAA CATCAAGCTA GGGCCCGCCC CTGAAAGATC
 1021 TACGCACGCA TCAGCATGCT CAAGGACTCC TAGAGTCTCT GCTGCCTGCT CACTGCACCA
 1081 CCAGGCAGCA GGATTCTAAC CCATGCAGGC ACGCTCGGGA TGGAAGCAGA GCTTCACGAC
1141 ATCTGAGGAG CCCAGGAAGG CCAGCCTTGG GCGGGAAAGG
//
Primer: ME-1290RV (Pr0046)
Region: insert 3'
Sequence file: RDB12787_A4Ekb.seq check
>12787_ME_1290Rv_C10_07.ab1
    1 ATGGGCATTT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA
   61 GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAG TTATCTAGAT CCGGTGGATC
  121 CCGGGCCCGC GGTACCGTCG ACTGCAGAAT TCGCGGCCGC GTCGACGCCG GTGCTATCAG
  181 TATTTACAAG CGCAAAGCCC CATCTTCTGT GCCACCTACC TCTACCCCTT CCACAGAAAT
  241 GGCCCCTGAG AGCCACTGAA GAGGTACTCG GACCTCCTCC CAGCTGCACC CCTAGGGAGA
  301 GCCCCTTCCC GCCAAGCTGG CCCTTTCCTG GGCTCCTCAG GAATGTCCGT GAGGCTCTGC
  361 TCCATCCGGA GGCGGTTGCC TGCATGGGGT TAGAATCCTG GCTGCCTGGT GGTGCAGTGG
  421 GCAGGCAGCA GAGACTCTAG GAGTCCTTGA GCATGCTGAT GCGTGCGTAG ATCTTCAGGG
  481 CGGGCCCTAG CTTGATGTTC ATGGCGCTCA TTAGGTGGTC CTCCTTGAGC AGCAGCAAGG
  541 CTTGCCCATC AATCTCCTGC GCACGGAACT CCTCCGCGAT CTCCTGGCAG CCTGGCAGAG
  601 AGCGGATGAA CTCATAGACA TCCTCTACAT TCCATTTTGT GGGCTCGCTC GGCAGGAAGT
  661 GGTGTCCCAC GCCCACAAGG TCCCTCATGT GCATGTCAGG GAGATCCAGG TCCCTCTGGC
  721 CTTGGCGCCG TCGCGAGGTG GACGAGCTGG CTGAGATGGG TGACAAGGGC TCCTCATAGC
  781 TGGAGTTATC TGAGCACCGG CTGGAGTCCT CCTGGCTGTG CGCCAGCTGC AAGGCGGCAG
  841 TAACTGAAAG GGGTACAGTG CCTGAGGGCT GCTTCTTGGT GTCCTTGGTG AGTGTGGGCA
  901 GACTGGCCTT GCTGGCCCGT CGGCGGTTGT GGGTTGTGGT CCCTGCCTTC TGCAGCTTGC
  961 TTCGGTCTGA GTGAAAAAAG TCCCACTCGT TTGGTGCATC CCACATTATA CCTCTTTGCA
 1021 CAAGCCAATG GAACAGAAGC GCTTGGAACG CTTGAACTTG TAGCAAGTCC ACCCGTTCAC
 1081 AGAGCTCACA CTTGAGTTGA GGGGAGTGCC TCCTCTTGGA TTCTTGCAGT AAGGCTCTCC
1141 AATCCTCTGA AGTCCGTGAT
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP, insert 5'
Sequence file: RDB12787_A4Ekc.seq check
>12787_CMV_Forward_A10_01.ab1
    1 TTGATCCACT TCCGCCCCAA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
  781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG
  841 CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCATGACC TCAGGGAACG GAAACTCTGC
  901 CTCCAGCATC GCCGGCACTG CCCCCCAGAA TGGTGAGAAT AAACCACCAC AGGCCATTGT
  961 GAAACCCCAA ATCCTGACGC ATGTTATCGA AAGGTTTTGT GATCCAGGAG GGGGCGGGAG
 1021 CCCTTTCCCG GTGGGACGCT CGTCTCTGCT GGTTAGGAAT CTCAAAAAGA AAGTATGCAC
 1081 AGGGGTTTCT GGCCTGAGAA GCCTCCACAG CAGACACACA ACATCAACCG AACTCAGAGA
1141 TTGGAGAAGC CCTAACTGGC CAAGAAAAAA TC
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB12787_A4Ekd.seq check
>12787_12787_B0FnF_SV40pro_ori_F_A12_03_ABI24.ab1
    1 NNNNNNNNNN NGCCGCCNCN GCCTCTGANC TATTCNNGAA GTAGTGAGGA GGCTTTTTTG
   61 GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT
  121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
  181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
  241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC
  301 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC
  361 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC
  421 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG
  481 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG
  541 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT
  601 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAN
  661 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC
  721 TTTTCTGGAT TCNTCGACNG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA NGACATAGCG
  781 TTGGCTACNC GNNATATTGC TGAAGANCTN GNCGGCGAAN GGGCTGANCG CTTCCTCNNG
  841 CTTTACNGTA TNGCCGCTCC CNANTCNCAG CGCATCGCCT TCNNNCGCCN TNTNNNNNAN
901 NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Isono, K., SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Dev. Cell 26 (6): 565-577 (2013). PMID 24091011. [link to RRC of NBRP]