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pGFP-Phc2

Probe for PcG complex. Expression clone of mouse Phc2 fused with GFP.

Catalog number RDB12787
Resource name pGFP-Phc2
Clone info. Expression clone of mouse Phc2 fused with GFP.
Comment Probe for PcG complex.
Vector backbone pEGFP-C2 (Plasmid)
Selectable markers Kan^r
Gene/insert name Mouse Phc2 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Koseki, Haruhiko | Isono, Kyoichi |
Other clones in our bank

External Database
Mouse Phc2 Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Developmental Cell, 26, 565-577(2013)).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Developmental Cell, 26, 565-577(2013))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB12787 pGFP-Phc2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Isono, K., SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Dev. Cell 26 (6): 565-577 (2013). PMID 24091011.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12787_A4Ekp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C (Pr0025)
          Region: EGFP, insert 5'
          Sequence file: RDB12787_A4Eka.seq check
          >12787_EGFP_C_B10_04.ab1
              1 AGGGGATATT CTCGGGGCAT GGGACGAGCT GTACAAGTCC GGCCGGACTC AGATCTCGAG
             61 CTCAAGCTTC GAATTCATGA CCTCAGGGAA CGGAAACTCT GCCTCCAGCA TCGCCGGCAC
            121 TGCCCCCCAG AATGGTGAGA ATAAACCACC ACAGGCCATT GTGAAACCCC AAATCCTGAC
            181 GCATGTTATC GAAGGGTTTG TGATCCAGGA GGGGGCGGAG CCTTTCCCGG TGGGACGCTC
            241 GTCCCTGCTG GTAGGGAATC TCAAAAAGAA GTATGCACAG GGGTTCTTGC CTGAGAAGCC
            301 TCCACAGCAG GACCACACCA CCACCACCGA CTCAGAGATG GAAGAGCCCT ACCTGCAAGA
            361 ATCCAAAGAG GAGGGCACTC CCCTCAAACT CAAGTGTGAG CTCTGTGGAC GGGTGGACTT
            421 TGCCTACAAG TTCAAGCGTT CCAAGCGCTT CTGTTCCATG GCTTGTGCAA AGAGGTATAA
            481 TGTGGGATGC ACCAAACGAG TGGGACTTTT TCACTCAGAC CGAAGCAAGC TGCAGAAGGC
            541 AGGGACCACA ACCCACAACC GCCGACGGGC CAGCAAGGCC AGTCTGCCCA CACTCACCAA
            601 GGACACCAAG AAGCAGCCCT CAGGCACTGT ACCCCTTTCA GTTACTGCCG CCTTGCAGCT
            661 GGCGCACAGC CAGGAGGACT CCAGCCGGTG CTCAGATAAC TCCAGCTATG AGGAGCCCTT
            721 GTCACCCATC TCAGCCAGCT CGTCCACCTC GCGACGGCGC CAAGGCCAGA GGGACCTGGA
            781 TCTCCCTGAC ATGCACATGA GGGACCTTGT GGGCGTGGGA CACCACTTCC TGCCGAGCGA
            841 GCCCACAAAA TGGAATGTAG AGGATGTCTA TGAGTTCATC CGCTCTCTGC CAGGCTGCCA
            901 GGAGATCGCG GAGGGAGTTC CGTGCGCAGG AGATTGATGG GCAAGCCTTG CTGCTGCTCA
            961 AGGGAGGACC ACCTAAATGA GCGCCATGAA CATCAAGCTA GGGCCCGCCC CTGAAAGATC
           1021 TACGCACGCA TCAGCATGCT CAAGGACTCC TAGAGTCTCT GCTGCCTGCT CACTGCACCA
           1081 CCAGGCAGCA GGATTCTAAC CCATGCAGGC ACGCTCGGGA TGGAAGCAGA GCTTCACGAC
          1141 ATCTGAGGAG CCCAGGAAGG CCAGCCTTGG GCGGGAAAGG
          //
          Primer: ME-1290RV (Pr0046)
          Region: insert 3'
          Sequence file: RDB12787_A4Ekb.seq check
          >12787_ME_1290Rv_C10_07.ab1
              1 ATGGGCATTT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA
             61 GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAG TTATCTAGAT CCGGTGGATC
            121 CCGGGCCCGC GGTACCGTCG ACTGCAGAAT TCGCGGCCGC GTCGACGCCG GTGCTATCAG
            181 TATTTACAAG CGCAAAGCCC CATCTTCTGT GCCACCTACC TCTACCCCTT CCACAGAAAT
            241 GGCCCCTGAG AGCCACTGAA GAGGTACTCG GACCTCCTCC CAGCTGCACC CCTAGGGAGA
            301 GCCCCTTCCC GCCAAGCTGG CCCTTTCCTG GGCTCCTCAG GAATGTCCGT GAGGCTCTGC
            361 TCCATCCGGA GGCGGTTGCC TGCATGGGGT TAGAATCCTG GCTGCCTGGT GGTGCAGTGG
            421 GCAGGCAGCA GAGACTCTAG GAGTCCTTGA GCATGCTGAT GCGTGCGTAG ATCTTCAGGG
            481 CGGGCCCTAG CTTGATGTTC ATGGCGCTCA TTAGGTGGTC CTCCTTGAGC AGCAGCAAGG
            541 CTTGCCCATC AATCTCCTGC GCACGGAACT CCTCCGCGAT CTCCTGGCAG CCTGGCAGAG
            601 AGCGGATGAA CTCATAGACA TCCTCTACAT TCCATTTTGT GGGCTCGCTC GGCAGGAAGT
            661 GGTGTCCCAC GCCCACAAGG TCCCTCATGT GCATGTCAGG GAGATCCAGG TCCCTCTGGC
            721 CTTGGCGCCG TCGCGAGGTG GACGAGCTGG CTGAGATGGG TGACAAGGGC TCCTCATAGC
            781 TGGAGTTATC TGAGCACCGG CTGGAGTCCT CCTGGCTGTG CGCCAGCTGC AAGGCGGCAG
            841 TAACTGAAAG GGGTACAGTG CCTGAGGGCT GCTTCTTGGT GTCCTTGGTG AGTGTGGGCA
            901 GACTGGCCTT GCTGGCCCGT CGGCGGTTGT GGGTTGTGGT CCCTGCCTTC TGCAGCTTGC
            961 TTCGGTCTGA GTGAAAAAAG TCCCACTCGT TTGGTGCATC CCACATTATA CCTCTTTGCA
           1021 CAAGCCAATG GAACAGAAGC GCTTGGAACG CTTGAACTTG TAGCAAGTCC ACCCGTTCAC
           1081 AGAGCTCACA CTTGAGTTGA GGGGAGTGCC TCCTCTTGGA TTCTTGCAGT AAGGCTCTCC
          1141 AATCCTCTGA AGTCCGTGAT
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, EGFP, insert 5'
          Sequence file: RDB12787_A4Ekc.seq check
          >12787_CMV_Forward_A10_01.ab1
              1 TTGATCCACT TCCGCCCCAA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
             61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT
            121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
            181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
            241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
            301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
            361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
            421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
            481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
            541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
            601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
            661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
            721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
            781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG
            841 CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCATGACC TCAGGGAACG GAAACTCTGC
            901 CTCCAGCATC GCCGGCACTG CCCCCCAGAA TGGTGAGAAT AAACCACCAC AGGCCATTGT
            961 GAAACCCCAA ATCCTGACGC ATGTTATCGA AAGGTTTTGT GATCCAGGAG GGGGCGGGAG
           1021 CCCTTTCCCG GTGGGACGCT CGTCTCTGCT GGTTAGGAAT CTCAAAAAGA AAGTATGCAC
           1081 AGGGGTTTCT GGCCTGAGAA GCCTCCACAG CAGACACACA ACATCAACCG AACTCAGAGA
          1141 TTGGAGAAGC CCTAACTGGC CAAGAAAAAA TC
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, NeoR_KanR
          Sequence file: RDB12787_A4Ekd.seq check
          >12787_12787_B0FnF_SV40pro_ori_F_A12_03_ABI24.ab1
              1 NNNNNNNNNN NGCCGCCNCN GCCTCTGANC TATTCNNGAA GTAGTGAGGA GGCTTTTTTG
             61 GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT
            121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
            181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
            241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC
            301 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC
            361 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC
            421 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG
            481 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG
            541 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT
            601 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAN
            661 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC
            721 TTTTCTGGAT TCNTCGACNG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA NGACATAGCG
            781 TTGGCTACNC GNNATATTGC TGAAGANCTN GNCGGCGAAN GGGCTGANCG CTTCCTCNNG
            841 CTTTACNGTA TNGCCGCTCC CNANTCNCAG CGCATCGCCT TCNNNCGCCN TNTNNNNNAN
          901 NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Isono, K., SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Dev. Cell 26 (6): 565-577 (2013). PMID 24091011.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl