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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3Flag-hp38beta2 (#RDB12359)

Expression vector of human p38beta MAPK cDNA, FLAG-tagged

Clone info. Expression vector of human p38beta MAPK cDNA, FLAG-tagged. Major form of p38b.
Comment This clone contains a cDNA fragment corresponding to the 89nt to 1183nt of NM_002751.7. Known differences (at least) : G to A substitution at 310nt (74a.a. Leu, synonym) ; C to T substitution at 586nt (166a.a. Ile, synonym) ; T to C substitution at 595nt (169a.a. Phe, synonym) ; A to G substitution at 844nt (252a.a. Ser, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Gene/insert name Human p38beta cDNA
Depositor|Developer Sudo, Tatsuhiko |
 

Distribution information

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RDB12359 pcDNA3Flag-hp38beta2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3Flag-hp38beta2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12359).

Reference section:

Kondoh, Y., Honda, K., Hiranuma, S., Hayashi, T., Shimizu, T., Watanabe, N., Osada, H., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12359_A4B5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert(FLAG,hp38beta 5')
Sequence file: RDB12359_A4B5a.seq check
>12359_12359_B2A7F_3_CMV-Forward_F01_16_ABI24.ab1
    1 NNNNNNNNNN CGNNNNNCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT CTCTGGCTAA
   61 CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC GACTCACTAT AGGGAGACCC
  121 AAGCTTGCCA CCATGGACTA CAAGGACGAC GACGATAAAT CGATGTCGGG CCCTCGCGCC
  181 GGCTTCTACC GGCAGGAGCT GAACAAGACC GTGTGGGAGG TGCCGCAGCG GCTGCAGGGG
  241 CTGCGCCCGG TGGGCTCCGG CGCCTACGGC TCCGTCTGTT CGGCCTACGA CGCCCGGCTG
  301 CGCCAGAAGG TGGCGGTGAA GAAGCTGTCG CGCCCCTTCC AGTCGCTGAT CCACGCGCGC
  361 AGAACGTACC GGGAGCTGCG GCTACTCAAG CACCTGAAGC ACGAGAACGT CATCGGGCTT
  421 CTGGACGTCT TCNNNNNGGC CACGTCCNNN NNNGACTTCA GCNANNTGTA CTTGNNGACC
  481 ACCCTGATGG GNNNNNANNN GNNNANCATC NNNNAGTGCC ANGCGCTGAN NGACNANCAC
541 GTTNCANTTT CCNGNNNNNN NNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA,insert(hp38 3')
Sequence file: RDB12359_A4B5b.seq check
>12359_12359_B2A7F_3_bGH_rev3_G01_19_ABI24.ab1
    1 NNNNNNNNNG NNNNNNNNNN GANGGCTGGC AACTAGAAGG CACAGTCGAG GCTGATCAGC
   61 GAGCTCTAGC ATTTAGGTGA CACTATAGAA TAGGGCCCTC TAGAGGGCTG CTGGGCAGCA
  121 CCTCACTGCT CAATCTCCAG GCTGCCAGGT GGCTTCGGTG GCTCTGGGGG CTTGAAGCTG
  181 AGGACTTCCT GGTAAGTGAG CTCCTTCCAC TCCTCCAGCG TGCGCTCCTT GGCCTCAACG
  241 CTCTCATCAT ATGGCTCGGC CTCTGGCTCA TCCTCGGGGT CGTGGTACTG GCTGAAGTAG
  301 GCGTGGGCCA GTGCCTCAGC TGCACTGACC CTCTGGTCAC TGTCCAGCAC CAGCATCCTT
  361 CCAAGGAGGT CTATGGCCAG GGGGTTGGCT CCACGGAAGA TGCTGCTCAG GTCCTTCTGG
  421 GGCATGGGGG GCAGGGACTG GATATATGTC CGGGCGTGTT CCGAGGAGAT TTTTGCCAGA
  481 ACCTCAGGGC TGGGTGTGCC CACCACTTCN NNNNNGCNNN TCAGCTGGNN NNTGTANTCG
  541 CTTCCCGGGA AGAGGGCCTT GCCCTGGANC NNNTCANNNN TGATGNNNNN NNNNGACNNN
  601 ATATCNNCTG TTNNNNNNNN ANGNNNNCNN NTTGAGCNTG ATCTCNNGTG NCCGGGNANN
661 NNNNNNNN
//
Primer: p38beta-M_R (Pr1101)
Region: insert mid(hp38 mid)
Sequence file: RDB12359_A4B5c.seq check
>12359_12359_B2A7F_3_p38beta-M_R_E03_15_ABI24.ab1
    1 GNNNNNNNNN NNNNNNNNNN NNTCCGGGCG TGTTCCGAGG AGANTTTTGC CNGAACCTNN
   61 GGGCTGGGTG TGCCCACCAC TTCCATGATG CGCTTCAGCT GGTCAATGTA GTCGCTTCCC
  121 GGGAAGAGGG CCTTGCCCTG GAGCAGCTCA GCCATGATGC AGCCCACGGA CCAGATATCC
  181 ACTGTTTGGT TGTAATGCAT CCAGTTGAGC ATGATCTCAG GTGCCCGGTA CCAGCGCGTG
  241 GCCACATAGC CGGTCATCTC CTCGTCCGCC TGGCGCGCCA GCCCGAAATC CAGAATCCTG
  301 AGCTCACAGT CCTCGTTCAC AGCCACGTTG CTGGGCTTCA GGTCCCGGTG GATGATCCCG
  361 GCCGAGTGGA TGTACTTCAG CCCGCGCAGC AGCTGGTAAA CCAGGAATTG AACGTGCTCG
  421 TCGCTCAGCG CCTGGCACTT GACGATGTTG TTCAGGTCGG CGCCCATCAG GGTGGTCACC
  481 AAGTACACTT CGCTGAAGTC CTCGATGGAC GTGGCCGGCG TGAAGACGTC NNGAAGCCCG
  541 ATGACGTTCT CGTGCTTCAG GTGCTTGAGT AGCCGCAGCT CCCGGTACGT TCTGCGCGCG
  601 TGGATCAGCG ACNGGAANGG GCGCGACNGN TTCTTCNCCG CCNCCTTCTG GCNNNNCCNG
661 GCGTNNNNNN NGANNNANNN ANCCNNNNNN NNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: NeoR
Sequence file: RDB12359_A4B5d.seq check
>12359_12359_B2A7F_3_SV40pro_ori_F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNTNNNNN AGCTATTCCN GAAGTAGTGA GGNNNGCTTT TTTGGAGGCC
   61 TANNGCTTTT GCAAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA
  121 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC
  181 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA
  241 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGNT CTTTTTGTCA AGACCNACCT
  301 GTCCGGNGCC CTGNNNGNNN NGNNNNNNNA NGCANCNCGN NNNTNNNGNN TGGNNNNNNN
361 NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kondoh, Y., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP]