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pcDNA3Flag-hp38beta2

Expression vector of human p38beta MAPK cDNA, FLAG-tagged

Catalog number RDB12359
Resource name pcDNA3Flag-hp38beta2
Clone info. Expression vector of human p38beta MAPK cDNA, FLAG-tagged. Major form of p38b.
Comment This clone contains a cDNA fragment corresponding to the 89nt to 1183nt of NM_002751.7. Known differences (at least) : G to A substitution at 310nt (74a.a. Leu, synonym) ; C to T substitution at 586nt (166a.a. Ile, synonym) ; T to C substitution at 595nt (169a.a. Phe, synonym) ; A to G substitution at 844nt (252a.a. Ser, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 ｋｂ
Gene/insert name Human p38beta cDNA
Depositor|Developer Sudo, Tatsuhiko |
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External Database
Human p38beta
          Reference sequence
            

          Distribution information

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          RDB12359 pcDNA3Flag-hp38beta2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kondoh, Y., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content MAPK p38 inhibitor screening (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12359_A4B5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert(FLAG,hp38beta 5')
          Sequence file: RDB12359_A4B5a.seq check
          >12359_12359_B2A7F_3_CMV-Forward_F01_16_ABI24.ab1
              1 NNNNNNNNNN CGNNNNNCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT CTCTGGCTAA
             61 CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC GACTCACTAT AGGGAGACCC
            121 AAGCTTGCCA CCATGGACTA CAAGGACGAC GACGATAAAT CGATGTCGGG CCCTCGCGCC
            181 GGCTTCTACC GGCAGGAGCT GAACAAGACC GTGTGGGAGG TGCCGCAGCG GCTGCAGGGG
            241 CTGCGCCCGG TGGGCTCCGG CGCCTACGGC TCCGTCTGTT CGGCCTACGA CGCCCGGCTG
            301 CGCCAGAAGG TGGCGGTGAA GAAGCTGTCG CGCCCCTTCC AGTCGCTGAT CCACGCGCGC
            361 AGAACGTACC GGGAGCTGCG GCTACTCAAG CACCTGAAGC ACGAGAACGT CATCGGGCTT
            421 CTGGACGTCT TCNNNNNGGC CACGTCCNNN NNNGACTTCA GCNANNTGTA CTTGNNGACC
            481 ACCCTGATGG GNNNNNANNN GNNNANCATC NNNNAGTGCC ANGCGCTGAN NGACNANCAC
          541 GTTNCANTTT CCNGNNNNNN NNNN
          //
          Primer: bGH_rev3
          Region: bGH pA,insert(hp38 3')
          Sequence file: RDB12359_A4B5b.seq check
          >12359_12359_B2A7F_3_bGH_rev3_G01_19_ABI24.ab1
              1 NNNNNNNNNG NNNNNNNNNN GANGGCTGGC AACTAGAAGG CACAGTCGAG GCTGATCAGC
             61 GAGCTCTAGC ATTTAGGTGA CACTATAGAA TAGGGCCCTC TAGAGGGCTG CTGGGCAGCA
            121 CCTCACTGCT CAATCTCCAG GCTGCCAGGT GGCTTCGGTG GCTCTGGGGG CTTGAAGCTG
            181 AGGACTTCCT GGTAAGTGAG CTCCTTCCAC TCCTCCAGCG TGCGCTCCTT GGCCTCAACG
            241 CTCTCATCAT ATGGCTCGGC CTCTGGCTCA TCCTCGGGGT CGTGGTACTG GCTGAAGTAG
            301 GCGTGGGCCA GTGCCTCAGC TGCACTGACC CTCTGGTCAC TGTCCAGCAC CAGCATCCTT
            361 CCAAGGAGGT CTATGGCCAG GGGGTTGGCT CCACGGAAGA TGCTGCTCAG GTCCTTCTGG
            421 GGCATGGGGG GCAGGGACTG GATATATGTC CGGGCGTGTT CCGAGGAGAT TTTTGCCAGA
            481 ACCTCAGGGC TGGGTGTGCC CACCACTTCN NNNNNGCNNN TCAGCTGGNN NNTGTANTCG
            541 CTTCCCGGGA AGAGGGCCTT GCCCTGGANC NNNTCANNNN TGATGNNNNN NNNNGACNNN
            601 ATATCNNCTG TTNNNNNNNN ANGNNNNCNN NTTGAGCNTG ATCTCNNGTG NCCGGGNANN
          661 NNNNNNNN
          //
          Primer: p38beta-M_R
          Region: insert mid(hp38 mid)
          Sequence file: RDB12359_A4B5c.seq check
          >12359_12359_B2A7F_3_p38beta-M_R_E03_15_ABI24.ab1
              1 GNNNNNNNNN NNNNNNNNNN NNTCCGGGCG TGTTCCGAGG AGANTTTTGC CNGAACCTNN
             61 GGGCTGGGTG TGCCCACCAC TTCCATGATG CGCTTCAGCT GGTCAATGTA GTCGCTTCCC
            121 GGGAAGAGGG CCTTGCCCTG GAGCAGCTCA GCCATGATGC AGCCCACGGA CCAGATATCC
            181 ACTGTTTGGT TGTAATGCAT CCAGTTGAGC ATGATCTCAG GTGCCCGGTA CCAGCGCGTG
            241 GCCACATAGC CGGTCATCTC CTCGTCCGCC TGGCGCGCCA GCCCGAAATC CAGAATCCTG
            301 AGCTCACAGT CCTCGTTCAC AGCCACGTTG CTGGGCTTCA GGTCCCGGTG GATGATCCCG
            361 GCCGAGTGGA TGTACTTCAG CCCGCGCAGC AGCTGGTAAA CCAGGAATTG AACGTGCTCG
            421 TCGCTCAGCG CCTGGCACTT GACGATGTTG TTCAGGTCGG CGCCCATCAG GGTGGTCACC
            481 AAGTACACTT CGCTGAAGTC CTCGATGGAC GTGGCCGGCG TGAAGACGTC NNGAAGCCCG
            541 ATGACGTTCT CGTGCTTCAG GTGCTTGAGT AGCCGCAGCT CCCGGTACGT TCTGCGCGCG
            601 TGGATCAGCG ACNGGAANGG GCGCGACNGN TTCTTCNCCG CCNCCTTCTG GCNNNNCCNG
          661 GCGTNNNNNN NGANNNANNN ANCCNNNNNN NNNN
          //
          Primer: SV40pro_ori_F
          Region: NeoR
          Sequence file: RDB12359_A4B5d.seq check
          >12359_12359_B2A7F_3_SV40pro_ori_F_H01_22_ABI24.ab1
              1 NNNNNNNNNN NNNNTNNNNN AGCTATTCCN GAAGTAGTGA GGNNNGCTTT TTTGGAGGCC
             61 TANNGCTTTT GCAAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA
            121 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC
            181 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA
            241 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGNT CTTTTTGTCA AGACCNACCT
            301 GTCCGGNGCC CTGNNNGNNN NGNNNNNNNA NGCANCNCGN NNNTNNNGNN TGGNNNNNNN
          361 NNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kondoh, Y., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl