Expression vector of human p38beta MAPK cDNA, FLAG-tagged
Clone info. | Expression vector of human p38beta MAPK cDNA, FLAG-tagged. Major form of p38b. |
---|---|
Comment | This clone contains a cDNA fragment corresponding to the 89nt to 1183nt of NM_002751.7. Known differences (at least) : G to A substitution at 310nt (74a.a. Leu, synonym) ; C to T substitution at 586nt (166a.a. Ile, synonym) ; T to C substitution at 595nt (169a.a. Phe, synonym) ; A to G substitution at 844nt (252a.a. Ser, synonym). |
Vector backbone | pcDNA3 (Plasmid) |
Size of vector backbone | 5.4 kb |
Gene/insert name | Human p38beta cDNA |
Depositor|Developer | Sudo, Tatsuhiko | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
---|---|
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Research purpose only. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
---|---|
MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 研究目的に限る。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB12359 | pcDNA3Flag-hp38beta2 | DNA solution |
Materials & Methods section:
The pcDNA3Flag-hp38beta2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12359). |
Reference section:
Kondoh, Y., Honda, K., Hiranuma, S., Hayashi, T., Shimizu, T., Watanabe, N., Osada, H., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB12359_A4B5p1-1.pdf |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro,insert(FLAG,hp38beta 5') Sequence file: RDB12359_A4B5a.seq |
---|
>12359_12359_B2A7F_3_CMV-Forward_F01_16_ABI24.ab1 1 NNNNNNNNNN CGNNNNNCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT CTCTGGCTAA 61 CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC GACTCACTAT AGGGAGACCC 121 AAGCTTGCCA CCATGGACTA CAAGGACGAC GACGATAAAT CGATGTCGGG CCCTCGCGCC 181 GGCTTCTACC GGCAGGAGCT GAACAAGACC GTGTGGGAGG TGCCGCAGCG GCTGCAGGGG 241 CTGCGCCCGG TGGGCTCCGG CGCCTACGGC TCCGTCTGTT CGGCCTACGA CGCCCGGCTG 301 CGCCAGAAGG TGGCGGTGAA GAAGCTGTCG CGCCCCTTCC AGTCGCTGAT CCACGCGCGC 361 AGAACGTACC GGGAGCTGCG GCTACTCAAG CACCTGAAGC ACGAGAACGT CATCGGGCTT 421 CTGGACGTCT TCNNNNNGGC CACGTCCNNN NNNGACTTCA GCNANNTGTA CTTGNNGACC 481 ACCCTGATGG GNNNNNANNN GNNNANCATC NNNNAGTGCC ANGCGCTGAN NGACNANCAC 541 GTTNCANTTT CCNGNNNNNN NNNN // |
Primer: bGH_rev3 (Pr0885) Region: bGH pA,insert(hp38 3') Sequence file: RDB12359_A4B5b.seq |
>12359_12359_B2A7F_3_bGH_rev3_G01_19_ABI24.ab1 1 NNNNNNNNNG NNNNNNNNNN GANGGCTGGC AACTAGAAGG CACAGTCGAG GCTGATCAGC 61 GAGCTCTAGC ATTTAGGTGA CACTATAGAA TAGGGCCCTC TAGAGGGCTG CTGGGCAGCA 121 CCTCACTGCT CAATCTCCAG GCTGCCAGGT GGCTTCGGTG GCTCTGGGGG CTTGAAGCTG 181 AGGACTTCCT GGTAAGTGAG CTCCTTCCAC TCCTCCAGCG TGCGCTCCTT GGCCTCAACG 241 CTCTCATCAT ATGGCTCGGC CTCTGGCTCA TCCTCGGGGT CGTGGTACTG GCTGAAGTAG 301 GCGTGGGCCA GTGCCTCAGC TGCACTGACC CTCTGGTCAC TGTCCAGCAC CAGCATCCTT 361 CCAAGGAGGT CTATGGCCAG GGGGTTGGCT CCACGGAAGA TGCTGCTCAG GTCCTTCTGG 421 GGCATGGGGG GCAGGGACTG GATATATGTC CGGGCGTGTT CCGAGGAGAT TTTTGCCAGA 481 ACCTCAGGGC TGGGTGTGCC CACCACTTCN NNNNNGCNNN TCAGCTGGNN NNTGTANTCG 541 CTTCCCGGGA AGAGGGCCTT GCCCTGGANC NNNTCANNNN TGATGNNNNN NNNNGACNNN 601 ATATCNNCTG TTNNNNNNNN ANGNNNNCNN NTTGAGCNTG ATCTCNNGTG NCCGGGNANN 661 NNNNNNNN // |
Primer: p38beta-M_R (Pr1101) Region: insert mid(hp38 mid) Sequence file: RDB12359_A4B5c.seq |
>12359_12359_B2A7F_3_p38beta-M_R_E03_15_ABI24.ab1 1 GNNNNNNNNN NNNNNNNNNN NNTCCGGGCG TGTTCCGAGG AGANTTTTGC CNGAACCTNN 61 GGGCTGGGTG TGCCCACCAC TTCCATGATG CGCTTCAGCT GGTCAATGTA GTCGCTTCCC 121 GGGAAGAGGG CCTTGCCCTG GAGCAGCTCA GCCATGATGC AGCCCACGGA CCAGATATCC 181 ACTGTTTGGT TGTAATGCAT CCAGTTGAGC ATGATCTCAG GTGCCCGGTA CCAGCGCGTG 241 GCCACATAGC CGGTCATCTC CTCGTCCGCC TGGCGCGCCA GCCCGAAATC CAGAATCCTG 301 AGCTCACAGT CCTCGTTCAC AGCCACGTTG CTGGGCTTCA GGTCCCGGTG GATGATCCCG 361 GCCGAGTGGA TGTACTTCAG CCCGCGCAGC AGCTGGTAAA CCAGGAATTG AACGTGCTCG 421 TCGCTCAGCG CCTGGCACTT GACGATGTTG TTCAGGTCGG CGCCCATCAG GGTGGTCACC 481 AAGTACACTT CGCTGAAGTC CTCGATGGAC GTGGCCGGCG TGAAGACGTC NNGAAGCCCG 541 ATGACGTTCT CGTGCTTCAG GTGCTTGAGT AGCCGCAGCT CCCGGTACGT TCTGCGCGCG 601 TGGATCAGCG ACNGGAANGG GCGCGACNGN TTCTTCNCCG CCNCCTTCTG GCNNNNCCNG 661 GCGTNNNNNN NGANNNANNN ANCCNNNNNN NNNN // |
Primer: SV40pro_ori_F (Pr0731) Region: NeoR Sequence file: RDB12359_A4B5d.seq |
>12359_12359_B2A7F_3_SV40pro_ori_F_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNTNNNNN AGCTATTCCN GAAGTAGTGA GGNNNGCTTT TTTGGAGGCC 61 TANNGCTTTT GCAAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA 121 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC 181 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA 241 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGNT CTTTTTGTCA AGACCNACCT 301 GTCCGGNGCC CTGNNNGNNN NGNNNNNNNA NGCANCNCGN NNNTNNNGNN TGGNNNNNNN 361 NNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kondoh, Y., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP] |
---|
Featured content