Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-Gata6-IP

Expression vector of mouse Gata6.

Catalog number RDB12110
Resource name pPyCAG-Gata6-IP
Alternative name stock no 20058
Clone info. Expression vector of mouse Gata6. Please note that this clone contains partial fragment corresponding 694 to 2041 of NM_010258.3 (cds, 272..2041).
SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20. Known difference: C to T substitution (Ala443Val) at a nucleotide corresponding 2037nt of NM_010258.3.
Vector backbone pPyCAG (Plasmid)
Gene/insert name Mouse Gata6 cDNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank mouse Gata6 (NCBI Gene 14465) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12110 pPyCAG-Gata6-IP DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB12110z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB12110p.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12110_A7Dep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB12110_A7Dea.seq check
>12110_12110_A7De_2_pAxCA_F1_B02_02_ABI08.ab1
    1 CCGTGACGGG CTACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
   61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCTA GTGATCGAGC AGCCGGAGGA
  121 AATGTACCAG ACCCTCGCCG CCCTGTCCAG CCAGGGGCCC GCCGCTTACG ACGGCGCGCC
  181 CGGCGGCTTC GTGCACTCCG CAGCGGCGGC GGCCGCTGCC GCCGCGGCAG CCAGCTCCCC
  241 GGTCTACGTG CCCACCACGC GCGTGGGCTC CATGCTGTCC GGCCTGCCCT ACCTTCAAGG
  301 GGCGGGCAGC GGGCCCAGCA ATCACGCGGG CGGAGCGGGT GCCCACCCAG GCTGGTCCCA
  361 GGCCTCCGCC GACAGCCCCC CGTATGGCGG GGGTGGCGCA GCCGGCGGCG GCGCGGCCGG
  421 ACCTGGAGGT GCGGGATCGG CTACGGCCCA CGCCTCTGCA CGCTTTCCCT ACTCGCCCAG
  481 CCCGCCCATG GCCAACGGCG CCGCGCGAGA CCCCGGGGGC TACGTGGCTG CGGGCGGCAC
  541 GGGCGCAGGC AGTGTGAGTG GAGGTGGCGG CAGCCTGGCG GCCATGGGTG GCCGGGAGCA
  601 CCAGTACAGC TCGCTGTCCG CAGCTCGGCC GCTGAACGGA ACGTACCACC ACCACCATCA
  661 CCATCACCCG ACCTACTCGC CCTACATGGC CGCACCGCTG ACTCCTGCCT GGCCAGCAGG
  721 ACCCTTCGAA ACGCCGGTGC TCCACAGCTT ACAGGGCCGC GCGGGAGCTC CACTCCCGGT
  781 GCCACGGGGC CCCAGCACAG ACCTGTTGGA GGACCTGTCG GAGAGCCGCG AGTGCGTGAA
  841 CTGCGGCTCC ATCCAGACGC CACTGTGGAG ACGAGACGGG CACCGGTCAT TACCTGTGCA
  901 ATGCATGCGG TCTCTACAGC AAGATGAATG GGCCTCAGCA GGCCCCTCAT CAAGCCCACA
  961 GAAGCGGCGT GCCTTTCATC ACGGCGGCTT GGACTTGTTC CTTGTTGCCA ACTTGTTCAC
 1021 ACCACACCAC TTACCTTTAT TGGCGTAGAA ATGGCTGAGG TGAGCCTGTG TTGCATGCTT
1081 GCCGGCTTCT ATTATATGAG CTTCTATGGG TTGACTTCGA CTCAC
//
Primer: CSII-CMV3'
Sequence file: RDB12110_A7Deb.seq check
>12110_12110_A7De_2_CSII-CMV3'_D02_04_ABI08.ab1
    1 CAAAGGAGAG AGGGGGGGGG GGCCGGAATT TGGCCGCGGG ATTTGGTAGC ACCAGCTCAG
   61 ACCAGGGCCA GAGCACACCA AGAATCCTGT CGCACGGAGG ATGTGACTTC GGCAGGGGAG
  121 GACAGACTGA CACCTATGTA GAGGCCGTCT TGACCTGAAT ACTTGAGGTC ACTGTTCTCG
  181 GGGTTGGCGT TTTCTCCCAC TGCAGACATC ACTGATGCCC CTACCCCTGA GGTGGTCGCT
  241 TGTGTAGAAG GAGAAGTATT TTTGGTGCAG TCATCTGAAT TAGAAGAAGA GGAAGTAGGA
  301 GTCATAGGGA CAGAGCCACT GCTGTTACCG GAGCAAGCTT TTGACTTATT TATATTTTTA
  361 GGTTTTCGTT TCCTGGTTTG AATTCCTTCT TTTTTCATAG CAAGTGGTCG AGGCACCCCA
  421 TGGAGTTTCA TATAGAGCCC GCAAGCATTG CACACAGGCT CACCCTCAGC ATTTCTACGC
  481 CATAAGGTAG TGGTTGTGGT GTGACAGTTG GCACAGGACA GTCCAAGCCG CCGTGATGAA
  541 GGCACGCGCT TCTGTGGCTT GATGAGGGGC CTGCTGAGGC CATTCATCTT GCTGTAGAGA
  601 CCGCATGCAT TGCACAGGTA ATGACCGGTG CCGTCTCGTC TCCACAGTGG CGTCTGGATG
  661 GAGCCGCAGT TCACGCACTC GCGGCTCTCC GACAGGTCCT CCAACAGGTC TGTGCTGGGG
  721 CCCCGTGGCA CCGGGAGTGG AGCTCCCGCG CGGCCCTGTA AGCTGTGGAG CACCGGCGTT
  781 TCGAAGGGTC CTGCTGGCCA GGCAGGAGTC AGCGGTGCGG CCATGTAGGG CGAGTAGGTC
841 GGGTGATGGT GATGGGTGGT GGTG
//
Primer: IRES-F
Sequence file: RDB12110_A7Dec.seq check
>12110_12110_A7De_2_IRES-F_F02_06_ABI08.ab1
    1 AAATTGCTTT TAATGGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG
   61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GACCGAGTAC
  121 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC
  181 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG
  241 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG
  301 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG
  361 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC
  421 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC
  481 CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG
  541 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG
  601 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG
  661 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC
  721 GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCATG CATCGATGAT CTAGAGCTCG
  781 CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT
  841 GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT
  901 TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGGTG GGGCAGGAAC
961 AGCAAGGGGG AAGGAATGGG GAAGA
//
Primer: Amp_R
Sequence file: RDB12110_A7Ded.seq check
>12110_12110_A7De_2_Amp_R_H02_08_ABI08.ab1
    1 ATCCCTTTTC CAATGACCGG CTTTGGAAAA CAAAGACTGT ATTTCCTGGA AATTAATGTT
   61 TATTCAATAA ACTGTGTATT CAGCTATATT CACATAGTGG TGAGGCTGAA ATGAGGCGGG
  121 AAGAGGCGGT TGGGGCTTAA TTATATCAAT TTGGGTGGCC CCACAGCGCC TCCAAGGCGC
  181 CAGTCCTGTT TTGACAAGTT GCCTCTGGAA GCCTCTCTTC TTTTTCTCCA GAGTAAGCGG
  241 AGGCCAGGGG CCCCCGGCCT CTGCTTAATA CTAAAAAAAA CAGCTGTTGT CATAGTAATG
  301 ATTGGGTGGA AACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA
  361 CACTGACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGC
  421 CCTCTGGAGG GCAGTTGCCT AGCAACTAAT TAAAAGAGGA TGTCGCACGG CCAGCTGCGG
  481 TCAGTTAGTC ACTTCCTGCT TAACTGACTT GACATTTTCT ATTTTAAGAG TCGGGAGGAA
  541 AATTACTGTG TTGGAGGCCC TCCGCCATCT TCTGAAGCTG AATCGAATTA ACTTGTTTAT
  601 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT
  661 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG
  721 GATCTGATAT CATCGTCGAC ATTTGATTAT TGACTAGGTT ATTAATAGTA ATCAATTACG
  781 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT TACATAACTT TACGGTAAAT
  841 GGGCCCGGCC TGGCTGACCG CCCAAACGAC CCCCGCCCAT TTGACGTCAA TAATGACGTA
  901 TGTTCCCATA GTAACGCCCA ATAGGGACTT TCCATTTGAC GTCAATGGGG TGGAGTATTT
  961 ACGGTAACTT GCCCACTTTG GCAGTACATC AAGTGGTATC ATATGCCAAG TTACGCCTCC
 1021 CCTTATTTGA CGTTCATGGA CGGTTAAAAT GGTCCGCCTT GCCCATATGT CCGGTTACAT
1081 GACTTAATGG AACTTTTCCC TACTGCAGTA CAACTTCTAT GACGGTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.06.22

GNP_filter3_RDBDEP_html_200316.pl