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pPyCAG-cGR-IP deltaN deltaS SVOri (#RDB10440)

Expression vector of glucocorticoid receptor ligand binding domain

Alternative name stock no 1435; pPyCAG-cGR-IP
Clone info. Expression vector of glucocorticoid receptor ligand binding domain.
Comment SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pPyCAG-IP (Plasmid)
Gene/insert name Human cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB10440z.seq
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (BMC Dev Biol. 7:80(2007)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (BMC Dev Biol. 7:80(2007))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB10440 pPyCAG-cGR-IP deltaN deltaS SVOri DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPyCAG-cGR-IP deltaN deltaS SVOri was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB10440).

Reference section:

Shimosato, D., Shiki, M., Niwa, H., Extra-embryonic endoderm cells derived from ES cells induced by GATA factors acquire the character of XEN cells. BMC Dev. Biol., 7, 80 (2007). PMID 17605826. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB10440_A6Gqp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB10440_A6Gqa.seq check
>10440_10440_A6Gq_6_pAxCA_F1_A12_03.ab1
    1 CTCACATTGT TGGGTCTGCT CATGTTATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA
   61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGA TCTCACGCGT
  121 GACTAGTGCC ATGGAAAATC CTGGTAACAA AACAATAGTT CCTGCAACGT TACCACAACT
  181 CACCCCTACC CTGGTGTCAC TGTTGGAGGT TATTGAACCT GAAGTGTTAT ATGCAGGATA
  241 TGATAGCTCT GTTCCAGACT CAACTTGGAG GATCATGACT ACGCTCAACA TGTTAGGAGG
  301 GCGGCAAGTG ATTGCAGCAG TGAAATGGGC AAAGGCAATA CCAGGTTTCA GGAACTTACA
  361 CCTGGATGAC CAAATGACCC TACTGCAGTA CTCCTGGATG TTTCTTATGG CATTTGCTCT
  421 GGGGTGGAGA TCATATAGAC AATCAAGTGC AAACCTGCTG TGTTTTGCTC CTGATCTGAT
  481 TATTAATGAG CAGAGAATGA CTCTACCCTG CATGTACGAC CAATGTAAAC ACATGCTGTA
  541 TGTTTCCTCT GAGTTACACA GGCTTCAGGT ATCTTATGAA GAGTATCTCT GTATGAAAAC
  601 CTTACTGCTT CTCTCTTCAG TTCCTAAGGA CGGTCTGAAG AGCCAAGAGC TATTTGATGA
  661 AATTAGAATG ACCTACATCA AAGAGCTAGG AAAAGCCATT GTCAAGAGGG AAGGAAACTC
  721 CAGCCAGAAC TGGCAGCGGT TTTATCAACT GACAAAACTC TTGGATTCTA TGCATGAAGT
  781 GGTTGAAAAT CTCCTTAACT ATTGCTTCCA AACATTTTTG GATAAGACCA TGTCCATCGA
  841 GTTCCCCGAG ATGTTAGCTG AAATCATCAC CAATCAGATA CCAAAATATT CAAATGGAAA
  901 TATCAAAAAA CTTCTGTTTC ATCAAAAGTG AGCGGCCGCT CGATAAGCTT GATATCGAAT
961 TCCGCCGCCT CCCCTTTTTG TCGAGTC
//
Primer: IRES-F
Sequence file: RDB10440_A6Gqb.seq check
>10440_10440_A6Gq_6_IRES-F_C12_09.ab1
    1 GGAGGCGTTC TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
  121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
  181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
  241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
  301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
  361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
  421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
  481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
  541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
  601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
  661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
  721 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATGATCTAGA GCTCGCTGAT
  781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
  841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
  901 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGGTG GGGTGGGGCA GGACAGCAAG
  961 GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGCTCT ATGGGCTTCT
 1021 GAGCGGAAAG AACCTGCAGC CCCAAGCTGC GTATCATGGG TCATAGCTGT TTCTGTGTGA
 1081 AATTGTATCC GCTCACATCC ACACAACCAT ACGAGTCGGC AAGGCAATAA AGGTGTTAGC
//
Primer: Amp_R
Sequence file: RDB10440_A6Gqc.seq check
>10440_10440_A6Gq_6_Amp_R_D12_12.ab1
    1 TTCGTTTCAT GACCGCTTTG GAAAACAAAG ACTGTATTTC CTGGAAATTA ATGTTTATTC
   61 AATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG
  121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC
  181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC
  241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG
  301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG
  361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT
  421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT
  481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA
  541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG
  601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT
  721 GATATCATCG TCGACATTGA TTATTGACTA GCTAGTTATT AATAGTAATC AATTACGGGG
  781 TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG
  841 CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA
  901 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC
  961 CACTTGGCAG TACATCAAGT GTATCATATG CCCAAGTACG CCCCCCTATT GACGTCATGA
 1021 CGGTAAATGG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGGA CTTTTCTACT
 1081 GGCAGTACAT CTACGTATAG TCATCGGCTA TTACAATGAA TGGATCGAGT GTGAAGCCCC
1141 CCG
//
Primer: BGHpA_F
Sequence file: RDB10440_A6Gqd.seq check
>10440_10440_A6Gq_6_BGHpA-F_E12_15.ab1
    1 TTGGAATGGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG
   61 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG
  121 AACCTGCAGC CCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
  181 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
  241 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
  301 AAACCTGTCG TGCCAGCGGA TCCGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
  361 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGCATGG
  421 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA
  481 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTAA CTTGTTTATT
  541 GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT
  601 TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG
  661 ATCCGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT
  721 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT
  781 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCACGAAAGA
  841 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT
  901 TTTTCCATAG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
  961 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCCTGGAAGC TCCCTCGTGC
 1021 GCTCTCCTGT TTCCGACCCT GCCGCCTTAC CGGATACCTG TCCGCCTTTT CTCCCTTTCA
 1081 GGAGCGTGAC GCTTTTCTCA TAGCTTCACG CTGGTAAGTA TCCTCAGGTT CGGGTTGTTA
1141 AGTCCGTTCC GCGCCTCTCC AG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shimosato, D., Extra-embryonic endoderm cells derived from ES cells induced by GATA factors acquire the character of XEN cells. BMC Dev. Biol., 7, 80 (2007). PMID 17605826. [link to RRC of NBRP]