icon below as well as clone information before placing your order.
Expression vector under the control of tet inducible promoter. Puromycin serectable
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Other clones in our bank
External Database Reference sequence |
| Sequence (full) |
RDB09003zzk01.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Cytotechnology, 63(4), 337-343 (2011)). |
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| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link] |
| Remarks | An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 63(4), 337-343 (2011))。 |
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| 提供依頼 | 提供依頼書 [Word ]本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| 備考 | クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB09003 | pMAK42 | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pMAK42 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09003). |
Reference section:
| Iwaki, T., Umemura, K., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Dnaconda's recommendation EXR0056e (English text) |
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| Featured content | Dnaconda's recommendation EXR0056j (Japanese text) |
| Featured content | Empty Backbone (English text) |
| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB09003_A3Fb.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: T7_22mer Sequence file: RDB09003_A3Fba.seq
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>09003_09003_A3Fb_T7_22mer_E06_15.ab1 1 TGCTGAGGCA GTGAAAATGC TTTATTTGTG AATTTGTGAT GCTATTGCTT TATTTGTAAC 61 CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT 121 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC 181 TGATTATGAT CCTCTAGAGA TATCGCTAGC ACGCGTTTCG AATCCGGAGC ATGCAGATCT 241 GGCGCGCCAC CGGTCTTAAG ATGCATGGTG GCGGCGATCC CCGGGTACCG AGCTCGAATT 301 CTCCAGGCGA TCTGACGGTT CACTAAACGA GCTCTGCTTA TATAGGCCTC CCACCGTACA 361 CGCCTACCTC GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA TACGTTCTCT 421 ATCACTGATA GGGATAAACT CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAC 481 ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC GTTCTCTATC ACTGATAGGG 541 AGTAAACTCG ACATCGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA CGTTCTCTAT 601 CACTGATAGG GAGTAAACTC GAGGAGCCCT AGGTTGGCCC ATTGCATACG TTGTATCCAT 661 ATCATAATAT GTACATTTAT ATTGGCTCAT GTCCAACATT ACCGCCATGT TGACATTGAT 721 TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG 781 AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC 841 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT 901 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC 961 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA TGGGCCGCCT GGCATTATGC 1021 CAGTACATGA CCTTATGGAA CTTTCTACTG GCAGTACATC TACGTATAGG TCATCGCTAA 1081 TACAATGTGA TGCGAATGGC AGTACATCCA TGGGCGGTGC AATAGGCCGG GTTTTGACGT 1141 CA // |
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Primer: SV40pA3'Element Reverse Sequence file: RDB09003_A3Fbb.seq
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>09003_09003_A3Fb_SV40pA3'Element Reverse_F06_18.ab1 1 GTAGGCAGGT GGGCGGAGCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA 121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG 181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG 241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA 301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT 361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG 421 CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA 481 GCTCACCCGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 541 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 601 TTATAAGCTG CAATAAACAA GTTCAAGCTG CGGCCGCCGT ATCGAGGTCG ACGGTATCGA 661 TAAGCTTGAT CTAGAGTCGG TGGGCCTCCT ACAGGAACAG GTGGTGGCGG CCCTCGGTGC 721 GCTCGTACTG CTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC AGCTTGGGCG 781 TCCACGTAGT AGTAGCCGGG CAGCTGCACG GGCTTCTTGG CCATGTAGAT GGACTTGAAC 841 TCCACCAGGT AGTGGCCCGC CGTCCTTCAG CTTCAGGGCC TTGTGGGGTC TCGCCCTTCA 901 GCACGCCGGT CGCGGGGGGG TACAGGCGCT CGGGTGGAGG CCTCCTCAGC CCATGGATCT 961 TTCTTCTGCA TCAACGGGGG TCGTCGAGGG GAAGTTCACG TCGATGAACC TTCACATTGT 1021 AGATGAGGCC AGTCGTTCCT GCAATGAAGA AGTCCTGGGT CACGATCGCT ACGTCGGC // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115. [link to RRC of NBRP] |
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2023.12.12
GNP_filter3_RDBDEP_html_231212.pl