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pMAK42

Expression vector under the control of tet inducible promoter. Puromycin serectable

Catalog number RDB09003
Resource name pMAK42
Clone info. Expression vector under the control of tet inducible promoter. Puromycin serectable.
Vector backbone pMAK42 (Plasmid)
Size of vector backbone 9.2 kb
Selectable markers Amp^r
Depositor|Developer Iwaki, Takayuki |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Cytotechnology, 63(4), 337-343 (2011)).
            Ordering Please visit Information of Request for Distribution.[link] 
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            Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
            Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
            The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 63(4), 337-343 (2011))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link]
            備考 クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。
            本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA(Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)をご参照ください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB09003 pMAK42 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB09003zzk01.seq provided by the depositor

            Original reference

            original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0056e (English text)
            Featured content Dnaconda's recommendation EXR0056j (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB09003_A3Fb.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_22mer
            Sequence file: RDB09003_A3Fba.seq check
            >09003_09003_A3Fb_T7_22mer_E06_15.ab1
                1 TGCTGAGGCA GTGAAAATGC TTTATTTGTG AATTTGTGAT GCTATTGCTT TATTTGTAAC
               61 CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT
              121 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
              181 TGATTATGAT CCTCTAGAGA TATCGCTAGC ACGCGTTTCG AATCCGGAGC ATGCAGATCT
              241 GGCGCGCCAC CGGTCTTAAG ATGCATGGTG GCGGCGATCC CCGGGTACCG AGCTCGAATT
              301 CTCCAGGCGA TCTGACGGTT CACTAAACGA GCTCTGCTTA TATAGGCCTC CCACCGTACA
              361 CGCCTACCTC GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA TACGTTCTCT
              421 ATCACTGATA GGGATAAACT CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAC
              481 ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC GTTCTCTATC ACTGATAGGG
              541 AGTAAACTCG ACATCGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA CGTTCTCTAT
              601 CACTGATAGG GAGTAAACTC GAGGAGCCCT AGGTTGGCCC ATTGCATACG TTGTATCCAT
              661 ATCATAATAT GTACATTTAT ATTGGCTCAT GTCCAACATT ACCGCCATGT TGACATTGAT
              721 TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG
              781 AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC
              841 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT
              901 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC
              961 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA TGGGCCGCCT GGCATTATGC
             1021 CAGTACATGA CCTTATGGAA CTTTCTACTG GCAGTACATC TACGTATAGG TCATCGCTAA
             1081 TACAATGTGA TGCGAATGGC AGTACATCCA TGGGCGGTGC AATAGGCCGG GTTTTGACGT
            1141 CA
            //
            Primer: SV40pA3'Element Reverse
            Sequence file: RDB09003_A3Fbb.seq check
            >09003_09003_A3Fb_SV40pA3'Element Reverse_F06_18.ab1
                1 GTAGGCAGGT GGGCGGAGCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
               61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
              121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
              181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
              241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
              301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
              361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
              421 CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA
              481 GCTCACCCGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA
              541 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA
              601 TTATAAGCTG CAATAAACAA GTTCAAGCTG CGGCCGCCGT ATCGAGGTCG ACGGTATCGA
              661 TAAGCTTGAT CTAGAGTCGG TGGGCCTCCT ACAGGAACAG GTGGTGGCGG CCCTCGGTGC
              721 GCTCGTACTG CTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC AGCTTGGGCG
              781 TCCACGTAGT AGTAGCCGGG CAGCTGCACG GGCTTCTTGG CCATGTAGAT GGACTTGAAC
              841 TCCACCAGGT AGTGGCCCGC CGTCCTTCAG CTTCAGGGCC TTGTGGGGTC TCGCCCTTCA
              901 GCACGCCGGT CGCGGGGGGG TACAGGCGCT CGGGTGGAGG CCTCCTCAGC CCATGGATCT
              961 TTCTTCTGCA TCAACGGGGG TCGTCGAGGG GAAGTTCACG TCGATGAACC TTCACATTGT
            1021 AGATGAGGCC AGTCGTTCCT GCAATGAAGA AGTCCTGGGT CACGATCGCT ACGTCGGC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl