Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMAK42

Expression vector under the control of tet inducible promoter. Puromycin serectable

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Cytotechnology, 63(4), 337-343 (2011)).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
Remarks	An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 63(4), 337-343 (2011))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。 Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB09003_A3Fb.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_22mer Sequence file: RDB09003_A3Fba.seq
>09003_09003_A3Fb_T7_22mer_E06_15.ab1     1 TGCTGAGGCA GTGAAAATGC TTTATTTGTG AATTTGTGAT GCTATTGCTT TATTTGTAAC    61 CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT   121 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC   181 TGATTATGAT CCTCTAGAGA TATCGCTAGC ACGCGTTTCG AATCCGGAGC ATGCAGATCT   241 GGCGCGCCAC CGGTCTTAAG ATGCATGGTG GCGGCGATCC CCGGGTACCG AGCTCGAATT   301 CTCCAGGCGA TCTGACGGTT CACTAAACGA GCTCTGCTTA TATAGGCCTC CCACCGTACA   361 CGCCTACCTC GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA TACGTTCTCT   421 ATCACTGATA GGGATAAACT CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAC   481 ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC GTTCTCTATC ACTGATAGGG   541 AGTAAACTCG ACATCGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA CGTTCTCTAT   601 CACTGATAGG GAGTAAACTC GAGGAGCCCT AGGTTGGCCC ATTGCATACG TTGTATCCAT   661 ATCATAATAT GTACATTTAT ATTGGCTCAT GTCCAACATT ACCGCCATGT TGACATTGAT   721 TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG   781 AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC   841 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT   901 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC   961 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA TGGGCCGCCT GGCATTATGC  1021 CAGTACATGA CCTTATGGAA CTTTCTACTG GCAGTACATC TACGTATAGG TCATCGCTAA  1081 TACAATGTGA TGCGAATGGC AGTACATCCA TGGGCGGTGC AATAGGCCGG GTTTTGACGT 1141 CA //
Primer: SV40pA3'Element Reverse Sequence file: RDB09003_A3Fbb.seq
>09003_09003_A3Fb_SV40pA3'Element Reverse_F06_18.ab1     1 GTAGGCAGGT GGGCGGAGCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA   121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG   181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG   241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA   301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT   361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG   421 CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA   481 GCTCACCCGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA   541 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA   601 TTATAAGCTG CAATAAACAA GTTCAAGCTG CGGCCGCCGT ATCGAGGTCG ACGGTATCGA   661 TAAGCTTGAT CTAGAGTCGG TGGGCCTCCT ACAGGAACAG GTGGTGGCGG CCCTCGGTGC   721 GCTCGTACTG CTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC AGCTTGGGCG   781 TCCACGTAGT AGTAGCCGGG CAGCTGCACG GGCTTCTTGG CCATGTAGAT GGACTTGAAC   841 TCCACCAGGT AGTGGCCCGC CGTCCTTCAG CTTCAGGGCC TTGTGGGGTC TCGCCCTTCA   901 GCACGCCGGT CGCGGGGGGG TACAGGCGCT CGGGTGGAGG CCTCCTCAGC CCATGGATCT   961 TTCTTCTGCA TCAACGGGGG TCGTCGAGGG GAAGTTCACG TCGATGAACC TTCACATTGT 1021 AGATGAGGCC AGTCGTTCCT GCAATGAAGA AGTCCTGGGT CACGATCGCT ACGTCGGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.27

GNP_filter3_RDBDEP_html_220727.pl