RDB09002 - pMAK17

Expression clone of EGFP under the control of tet inducible promoter. Puromycin serectable

Distribution information

Sequence (full)	RDB09002zzk01.seq provided by the depositor

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Cytotechnology, 63(4), 337-343 (2011)).
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]). This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 63(4), 337-343 (2011))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。 GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします（Schedule AとSchedule Bを入手[link])。
備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。 Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

References and tips

QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB09002	pMAK17	DNA solution

original	Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115.

Featured content	Dnaconda's recommendation EXR0056e (English text)
Featured content	Dnaconda's recommendation EXR0056j (Japanese text)
Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: T7_22mer Sequence file: RDB09002_A3Fba.seq
>09002_09002_A3Fb_T7_22mer_C06_09.ab1 1 TGGCGGAAGC AGTGAAAATG CTTTATTTGT GAATTTGTGA TGCTATTGCT TTATTTGTAA 61 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 121 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG 181 CTGATTATGA TCCTCTAGAG ATATCGTCGA CAAGCTTATC GATGCGGCCT TACTTGTACA 241 GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT 301 CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG 361 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA 421 CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG TTCTTCTGCT 481 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA 541 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC 601 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC 661 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT 721 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGTCAGGTGG TCACGAGGGT GGGCCAGGGC 781 ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTTCAGGGTC AGCTTGCCGT AGTGGCATCG 841 CCCTCGGCCC TCCGCCGACA CGCTGAACTT GTGGCCGTTA CGTCGCCGTC AGCTCGACAG 901 GATGGCACCA CCCCGTGACA GCTCTCGCCC TGCTCACCAT GGTGGCGGCG ATCCGGGTAC 961 GAGCTCGATC CAAGGCGAAT TGACGTCCAT ACGGATCTTG CTTAATAGCT CACGTTACGG 1021 CCTACCTGCA ATCGTCCTAT AATGATGGAA TAATC //
Primer: SV40pA3'Element Reverse Sequence file: RDB09002_A3Fbb.seq
>09002_09002_A3Fb_SV40pA3'Element Reverse._D06_12.ab1 1 GGAGTCAAGG GGGGCGGAGC TATGGAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 241 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 301 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 361 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 421 AGCTATGACC ATGATTACGC CAAGCGCGCA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG 481 GAGCTCACCC GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG 541 AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC 601 CATTATAAGC TGCAATAAAC AAGTTCAAGC TGCGGCCGCC GTATCGAGGT CGACGGTATC 661 GATAAGCTTG ATCTAGAGTC GGTGGGCCTC CTACAGGAAC AGGTGGTGGC GGCCCTCGGT 721 GCGCTCGTAC TGCTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAGCTTGGC 781 GTCCACGTAG TAGTAGCCGG GCAGCTGCAC GGGCTTCTTG GCCATGTAGA TGGACTTGAA 841 CTCCACCAGG TAGTGGCCGC CGTCCTTCAG CTTCAGGGCC TTGTGGGTCT CGCCCTTCAG 901 CACGCCGTCG CGGGGGGTAC AGGCGCTCGG TGGAGGCCCT CCCAGCCCAT GGTCTTCTTC 961 TGCATCACGG GGCCGTCGGA GGGGAAAGTT CACGCCCGAT GAACTTCACC TTGTAGATGA 1021 GCAGCCGTCC TGCAGGGAGA GTCCTGGGGT CACGGGTCGC CACCGCCGCG GTTCTCGAGT 1081 CCATCACGCG CTCACTGGAG TCCTCGGGCA GACAGCTCTT GATCGGATGT CCGATCGGAA 1141 GACCTCA //