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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMAK17

Expression clone of EGFP under the control of tet inducible promoter. Puromycin serectable

Catalog number RDB09002
Resource name pMAK17
Clone info. Expression clone of EGFP under the control of tet inducible promoter. Puromycin serectable.
Vector backbone pMAK42 (Plasmid)
Size of vector backbone 9.2 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Iwaki, Takayuki |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence (full) RDB09002zzk01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Cytotechnology, 63(4), 337-343 (2011)).
            Ordering Please visit Information of Request for Distribution.[link] 
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            This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
            This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 63(4), 337-343 (2011))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。
            Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB09002 pMAK17 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0056e (English text)
            Featured content Dnaconda's recommendation EXR0056j (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB09002_A3Fb.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_22mer
            Sequence file: RDB09002_A3Fba.seq check
            >09002_09002_A3Fb_T7_22mer_C06_09.ab1
                1 TGGCGGAAGC AGTGAAAATG CTTTATTTGT GAATTTGTGA TGCTATTGCT TTATTTGTAA
               61 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
              121 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
              181 CTGATTATGA TCCTCTAGAG ATATCGTCGA CAAGCTTATC GATGCGGCCT TACTTGTACA
              241 GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT
              301 CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG
              361 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA
              421 CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG TTCTTCTGCT
              481 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
              541 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
              601 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
              661 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
              721 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGTCAGGTGG TCACGAGGGT GGGCCAGGGC
              781 ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTTCAGGGTC AGCTTGCCGT AGTGGCATCG
              841 CCCTCGGCCC TCCGCCGACA CGCTGAACTT GTGGCCGTTA CGTCGCCGTC AGCTCGACAG
              901 GATGGCACCA CCCCGTGACA GCTCTCGCCC TGCTCACCAT GGTGGCGGCG ATCCGGGTAC
              961 GAGCTCGATC CAAGGCGAAT TGACGTCCAT ACGGATCTTG CTTAATAGCT CACGTTACGG
            1021 CCTACCTGCA ATCGTCCTAT AATGATGGAA TAATC
            //
            Primer: SV40pA3'Element Reverse
            Sequence file: RDB09002_A3Fbb.seq check
            >09002_09002_A3Fb_SV40pA3'Element Reverse._D06_12.ab1
                1 GGAGTCAAGG GGGGCGGAGC TATGGAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
               61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
              121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
              181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
              241 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT
              301 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT
              361 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC
              421 AGCTATGACC ATGATTACGC CAAGCGCGCA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG
              481 GAGCTCACCC GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG
              541 AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC
              601 CATTATAAGC TGCAATAAAC AAGTTCAAGC TGCGGCCGCC GTATCGAGGT CGACGGTATC
              661 GATAAGCTTG ATCTAGAGTC GGTGGGCCTC CTACAGGAAC AGGTGGTGGC GGCCCTCGGT
              721 GCGCTCGTAC TGCTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAGCTTGGC
              781 GTCCACGTAG TAGTAGCCGG GCAGCTGCAC GGGCTTCTTG GCCATGTAGA TGGACTTGAA
              841 CTCCACCAGG TAGTGGCCGC CGTCCTTCAG CTTCAGGGCC TTGTGGGTCT CGCCCTTCAG
              901 CACGCCGTCG CGGGGGGTAC AGGCGCTCGG TGGAGGCCCT CCCAGCCCAT GGTCTTCTTC
              961 TGCATCACGG GGCCGTCGGA GGGGAAAGTT CACGCCCGAT GAACTTCACC TTGTAGATGA
             1021 GCAGCCGTCC TGCAGGGAGA GTCCTGGGGT CACGGGTCGC CACCGCCGCG GTTCTCGAGT
             1081 CCATCACGCG CTCACTGGAG TCCTCGGGCA GACAGCTCTT GATCGGATGT CCGATCGGAA
            1141 GACCTCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl