icon below as well as clone information before placing your order.
Expression clone of EGFP under the control of tet inducible promoter. Puromycin serectable
|
Other clones in our bank
External Database Aequorea victoria GFP Reference sequence |
| Sequence (full) |
RDB09002zzk01.seq provided by the depositor |
|---|
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Cytotechnology, 63(4), 337-343 (2011)). |
|---|---|
| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link] |
| Remarks | An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 63(4), 337-343 (2011))。 |
|---|---|
| 提供依頼 | 提供依頼書 [Word ]本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| 備考 | クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB09002 | pMAK17 | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pMAK17 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09002). |
Reference section:
| Iwaki, T., Umemura, K., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Dnaconda's recommendation EXR0056e (English text) |
|---|---|
| Featured content | Dnaconda's recommendation EXR0056j (Japanese text) |
| Featured content | Empty Backbone (English text) |
| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB09002_A3Fb.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
|
Primer: T7_22mer Sequence file: RDB09002_A3Fba.seq
|
|---|
|
>09002_09002_A3Fb_T7_22mer_C06_09.ab1 1 TGGCGGAAGC AGTGAAAATG CTTTATTTGT GAATTTGTGA TGCTATTGCT TTATTTGTAA 61 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG 121 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG 181 CTGATTATGA TCCTCTAGAG ATATCGTCGA CAAGCTTATC GATGCGGCCT TACTTGTACA 241 GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT 301 CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG 361 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA 421 CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG TTCTTCTGCT 481 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA 541 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC 601 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC 661 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT 721 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGTCAGGTGG TCACGAGGGT GGGCCAGGGC 781 ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTTCAGGGTC AGCTTGCCGT AGTGGCATCG 841 CCCTCGGCCC TCCGCCGACA CGCTGAACTT GTGGCCGTTA CGTCGCCGTC AGCTCGACAG 901 GATGGCACCA CCCCGTGACA GCTCTCGCCC TGCTCACCAT GGTGGCGGCG ATCCGGGTAC 961 GAGCTCGATC CAAGGCGAAT TGACGTCCAT ACGGATCTTG CTTAATAGCT CACGTTACGG 1021 CCTACCTGCA ATCGTCCTAT AATGATGGAA TAATC // |
|
Primer: SV40pA3'Element Reverse Sequence file: RDB09002_A3Fbb.seq
|
|
>09002_09002_A3Fb_SV40pA3'Element Reverse._D06_12.ab1 1 GGAGTCAAGG GGGGCGGAGC TATGGAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 241 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 301 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 361 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 421 AGCTATGACC ATGATTACGC CAAGCGCGCA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG 481 GAGCTCACCC GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG 541 AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC 601 CATTATAAGC TGCAATAAAC AAGTTCAAGC TGCGGCCGCC GTATCGAGGT CGACGGTATC 661 GATAAGCTTG ATCTAGAGTC GGTGGGCCTC CTACAGGAAC AGGTGGTGGC GGCCCTCGGT 721 GCGCTCGTAC TGCTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAGCTTGGC 781 GTCCACGTAG TAGTAGCCGG GCAGCTGCAC GGGCTTCTTG GCCATGTAGA TGGACTTGAA 841 CTCCACCAGG TAGTGGCCGC CGTCCTTCAG CTTCAGGGCC TTGTGGGTCT CGCCCTTCAG 901 CACGCCGTCG CGGGGGGTAC AGGCGCTCGG TGGAGGCCCT CCCAGCCCAT GGTCTTCTTC 961 TGCATCACGG GGCCGTCGGA GGGGAAAGTT CACGCCCGAT GAACTTCACC TTGTAGATGA 1021 GCAGCCGTCC TGCAGGGAGA GTCCTGGGGT CACGGGTCGC CACCGCCGCG GTTCTCGAGT 1081 CCATCACGCG CTCACTGGAG TCCTCGGGCA GACAGCTCTT GATCGGATGT CCGATCGGAA 1141 GACCTCA // |
Please visit 
Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115. [link to RRC of NBRP] |
|---|
2023.12.12
GNP_filter3_RDBDEP_html_231212.pl