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pPyCAG-EGFP-IZ

Expression clone of GFP under the control of CAG promoter, zeocin resistance.

Catalog number RDB08526
Resource name pPyCAG-EGFP-IZ
Alternative name stock no 1256
Clone info. Expression clone of GFP under the control of CAG promoter, zeocin resistance.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 6.4 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Cell, 123, 917-929 (2005)).
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Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08526 pPyCAG-EGFP-IZ DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08526z.seq from Depositor
Electronic file Map RDB08526a.gif from Depositor

Featured content

References

original Niwa, H., Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell, 123, 917-929 (2005). PMID 16325584.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08526_A4Ec.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB08526_A4Eca.seq
>08526_08526_A4Ec_pAxCA_F1_F05_17.ab1
    1 TTAGGGGGGG CCGCCGTCAA CGGGGGTCCG CACATGGGTG TGCCTTCTTC TTTTTCCTAC
   61 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGCCTCATCA TTTTGGCAAA GAATTCCTCG
  121 AGATCCATTG TGGCCAGTGC GGCCGATCCC CGGGTACCGG TCGCCACCAT GGTGAGCAAG
  181 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  241 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  301 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  361 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  421 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  481 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  541 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  601 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
  661 AACTTCAAGA TCCGCCACAA CATCGAGGAC TGCAGCGTGC AGCTCGCCGA CCACTACCAG
  721 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC
  781 CAGTCCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTTC CTGCTGGAGT
  841 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA AGCGGCCGGC
901 CGCACTGGCC ACAATGGATC TCGAGGAATT CCGCCCCCCC CCCCCCTCTC CCCTCCCC
//
Primer: IRES-R RDB08526_A4Ecb.seq
>08526_08526_A4Ec_IRES-R_C03_09.ab1
    1 GGCAAAAGCG GGCTTCGGGC AGTACGTTAG GGGGGGGGGA GGGAGAGGGG GGGGGGGCGG
   61 AATTCCTCGA GATCCCTTGT GGCCAGTGCG GCCGGCCGCT TTACTTGTAC AGCTCGTCCA
  121 TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT
  181 CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGCAGCA
  241 CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT
  301 CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC GTTCTTCTGC TTGTCGGCCA
  361 TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT
  421 CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT
  481 TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA
  541 CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC
  601 GGCTGAAGCA CTGCACGCCG TAAGTCAGGG TGGTTCACGA GGGTGGGCCA GGGCACGGGC
  661 AGCTTGCCGG TGGTGCAGAT GAACTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG
721 CCCTCGCCGG ACACGCCTGA ACTTGTGGGC CGTTTA
//
Primer: Amp_R RDB08526_A4Ecc.seq
>08526_08526_A4Ec_Amp_R_H05_23.ab1
    1 ACTTTCCTTG GAAGCCTCTT CTTCTTTTTT CTCCAAATAA GCGGAGGCCA GGGGCCCCCG
   61 GCCTCTGCTT AATACTAAAA AAAACAGCTG TTGTCATAGT AATGATTGGG TGGAAACATT
  121 CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGA CATTCCAGGC
  181 CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA CTGCCCTCTG GAGGGCAGTT
  241 GCCTACCAAC TAATTAAAAG AGGATGTCGC ACGGCCAGCT GCGGTCAGTT AGTCACTTCC
  301 TGCTTAACTG ACTTGACATT TTCTATTTTA AGAGTCGGGA GGAAAATTAC TGTGTTGGAG
  361 GCCCTCCGCC ATCTTCTGAA GCTGAATCGA ATTAACTTGT TTATTGCAAC TTATAATGGT
  421 TACAAATTAA GCAATAGCAT CACAAATTTC ACAAATAAAA CCATTTTTTT CACTGCATTC
  481 TATTTGTGGT TTGTCCCAAC TCATCAATGT ATCTTATCAT GTCTGGATCT GATATCATCG
541 TCCACATTGA TTATTGACTC GTTATTAATA TTAATCAATT ACGGGGGTCA TT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Niwa, H., Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell, 123, 917-929 (2005). PMID 16325584.
reference Tran, T.H.Y., Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Biochem. Biophys. Rep. 15: 86-92 (2018). PMID 30094351.
user report Ohhata, T., Histone H3 lysine 36 tri-methylation is established over the Xist promoter by antisense Tsix transcription and contributes to repressing Xist expression. Mol. Cell. Biol. 35 (22): 3909-3920 (2015). PMID 26370508.

2018.12.11

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