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pPyCAG-EGFP-IZ

Expression clone of GFP under the control of CAG promoter, zeocin resistance.

Catalog number RDB08526
Resource name pPyCAG-EGFP-IZ
Alternative name stock no 1256
Clone info. Expression clone of GFP under the control of CAG promoter, zeocin resistance.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 6.4 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Cell, 123, 917-929 (2005)).
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] 
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            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cell, 123, 917-929 (2005))。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08526 pPyCAG-EGFP-IZ DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB08526z.seq
            Remarks, protocol and/or map (gif) RDB08526a.gif

            Original reference

            original Niwa, H., Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell, 123, 917-929 (2005). PMID 16325584.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08526_A8Etp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Sequence file: RDB08526_A8Eta.seq check
            >08526_08526_A8Et_pAxCA_F1_F04_16_ABI24.ab1
                1 CATCCCTGTC TACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
               61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCCTCG AGATCCATTG TGGCCAGTGC
              121 GGCCGATCCC CGGGTACCGG TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG
              181 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC
              241 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC
              301 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG
              361 CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA
              421 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC
              481 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT
              541 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT
              601 CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA
              661 CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA
              721 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA
              781 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
              841 TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGGCCG CACTGGCCAC AATGGATCTC
            901 GAGGAATTCC GCCCCCCCCC CCCCCCTTCT CCC
            //
            Primer: IRES-R
            Sequence file: RDB08526_A8Etb.seq check
            >08526_08526_A8Et_IRES-R_G04_19_ABI24.ab1
                1 GCAAGCGGCT TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGGGGGG GGCGGAATTC
               61 CTCGAGATCC ATTGTGGCCA GTGCGGCCGG CCGCTTTACT TGTACAGCTC GTCCATGCCG
              121 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG
              181 GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG
              241 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG
              301 ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA
              361 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC
              421 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC
              481 TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG
              541 CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG
              601 AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG
              661 CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC CTCGCCCTCG
              721 CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
              781 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTAC CCGGGGATCG
              841 GCCGCACTGG CCACAATGGA TCTCGAGGAA TTCTTTGCCA AAATGATGAG ACAGCACAAT
              901 AACCAGCACG TTGCCCAGGG AGCTGTAGGA AAAGAAGAAG GCATGAACAT GGTTTAGCAG
              961 AGGCTCTAGA GCCGCCGGTC ACACGGCCAG AGCGAAACCC CGCCTGCCCC GTCCCCCCGG
             1021 AAGCAGCCGT CCCCCCGCGG ACCGCCCGGA GCTGGAGAAG GAGAGGGACG CGGCCGCGAC
            1081 GCACGAAGGC CATTG
            //
            Primer: pCA-bgter
            Sequence file: RDB08526_A8Etc.seq check
            >08526_08526_A8Et_pCA-bgter_H04_22_ABI24.ab1
                1 AAAATGGGGG TCCCCATAAA TTTTTTGGCA GAGGGAAAAG ATCGAACCCC CCTCGGAACC
               61 CGTCGGGCCG CGTCGGACCG GCGGTGTTGG TCGGCGTCGG TCAGTCCTGC TCCTCGGCCA
              121 CGAAGTGCAC GCAGTTGCCG GCCGGGTCGC GCAGGGCGAA CTCCCGCCCC CACGGCTGCT
              181 CGCCGATCTC GGTCATGGCC GGCCCGGAGG CGTCCCGGAA GTTCGTGGAC ACGACCTCCG
              241 ACCACTCGGC GTACAGCTCG TCCAGGCCGC GCACCCACAC CCAGGCCAGG GTGTTGTCCG
              301 GCACCACCTG GTCCTGGACC GCGCTGATGA ACAGGGTCAC GTCGTCCCGG ACCACACCGG
              361 CGAAGTCGTC CTCCACGAAG TCCCGGGAGA ACCCGAGCCG GTCGGTCCAG AACTCGACCG
              421 CTCCGGCGAC GTCGCGCGCG GTGAGCACCG GAACGGCACT GGTCAACTTG GCCATGGTTG
              481 TGGCCATATT ATCATCGTGT TTTTCAAAGG AAAACCACGT CCCCGTGGTT CGGGGGGCCT
              541 AGACGTTTTT TTAACCTCGA CTAAACACAT GTAAAGCATG TGCACCGAGG CCCCAGATCA
              601 GATCCCATAC AATGGGGTAC CTTCTGGGCA TCCTTCAGCC CCTTGTTGAA ATACGCTTGA
              661 GGAGAGCCAT TTGACTCTTT CCACAACTAT CCAACTCACA ACGTGGCACT GGGGTTGTGC
              721 CGCCTTTGCA GGTGTATCTT ATACACGTGG CTTTTGGCCG CAGAGGCACC TGTCGCCAGG
              781 TGGGGGGGTT CCGCTGCCTG CAAAGGGTCG CTACAGACGT TGTTTGTCTT TCAGAAGCTT
              841 TCCAGAGGAA CTGCTTCCTT CACGACATTC AACAGACCTT TGCATTCCTT TGGCGAGAGG
              901 GGAAAGACCC CCTAAGGAAA TGCCTCGTCA AAGAAGAACA GGGCCCAAGG TTTTCCGGTC
              961 CTCACATGCC CAAAAGAACG CATATGGTGA AAATAACATA ATAGGATAGC GGCACACGCT
            1021 TATTCCAAGC GCTCGTCAGT TAACGTTAAG GGGAAAGGGG AAGAGG
            //
            Primer: SV40pA3'element Reverse
            Sequence file: RDB08526_A8Etd.seq check
            >08526_08526_A8Et_SV40pA3'ElementReverse_A05_02_ABI24.ab1
                1 GTGCTTGGGG GGCGGACCTA TGGAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
               61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
              121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
              181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
              241 TGGCCGATTC ATTAATGCAG CGGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA
              301 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC
              361 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAGC TTTTTGCAAA AGCCTAGGCC
              421 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC
              481 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT
              541 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCGG
              601 ATCCGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT
              661 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG
              721 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC
              781 GCCAAGCTTG GGCTGCAGGT CGAGGGATCT CCATAAGAGA AGAGGGACAG CTATGACTGG
              841 GAGTAGTCAG GAGAGGAGGA AAAATCTGGC TAGTAAAACA TGTAAGGAAA ATTTTAGGGA
              901 TGTTAAAGAA AAAAAAATAA CACAAAAACA AAATATAAAA AAAATCTAAA CCCTCAAAGT
              961 CAAAGGCTTT TCTATGGGAA ATAAAGAAAT GGGACAGCAC GGGGGCTGTT TTCATATACT
             1021 GATGGACCCT CTTTATAGCC CAACCCTTTG TTTCATTGGC AGGTCAGGCA TATGGGGTCA
            1081 TATGGTGCTA ACTTCTAAAC CAAAATACTC CATTTCCT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Niwa, H., Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell, 123, 917-929 (2005). PMID 16325584.
            reference Tran, T.H.Y., Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Biochem. Biophys. Rep. 15: 86-92 (2018). PMID 30094351.
            user report Ohhata, T., Histone H3 lysine 36 tri-methylation is established over the Xist promoter by antisense Tsix transcription and contributes to repressing Xist expression. Mol. Cell. Biol. 35 (22): 3909-3920 (2015). PMID 26370508.

            2022.02.09

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