Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-EGFP-IZ

Expression clone of GFP under the control of CAG promoter, zeocin resistance.

Sequence	RDB08526z.seq
Remarks, protocol and/or map (gif)	RDB08526a.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Cell, 123, 917-929 (2005)). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cell, 123, 917-929 (2005))。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08526_A8Etp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB08526_A8Eta.seq
>08526_08526_A8Et_pAxCA_F1_F04_16_ABI24.ab1     1 CATCCCTGTC TACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG    61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCCTCG AGATCCATTG TGGCCAGTGC   121 GGCCGATCCC CGGGTACCGG TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG   181 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC   241 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC   301 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG   361 CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA   421 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC   481 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT   541 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT   601 CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA   661 CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA   721 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA   781 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC   841 TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGGCCG CACTGGCCAC AATGGATCTC 901 GAGGAATTCC GCCCCCCCCC CCCCCCTTCT CCC //
Primer: IRES-R Sequence file: RDB08526_A8Etb.seq
>08526_08526_A8Et_IRES-R_G04_19_ABI24.ab1     1 GCAAGCGGCT TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGGGGGG GGCGGAATTC    61 CTCGAGATCC ATTGTGGCCA GTGCGGCCGG CCGCTTTACT TGTACAGCTC GTCCATGCCG   121 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG   181 GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG   241 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG   301 ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA   361 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC   421 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC   481 TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG   541 CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG   601 AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG   661 CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC CTCGCCCTCG   721 CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC   781 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTAC CCGGGGATCG   841 GCCGCACTGG CCACAATGGA TCTCGAGGAA TTCTTTGCCA AAATGATGAG ACAGCACAAT   901 AACCAGCACG TTGCCCAGGG AGCTGTAGGA AAAGAAGAAG GCATGAACAT GGTTTAGCAG   961 AGGCTCTAGA GCCGCCGGTC ACACGGCCAG AGCGAAACCC CGCCTGCCCC GTCCCCCCGG  1021 AAGCAGCCGT CCCCCCGCGG ACCGCCCGGA GCTGGAGAAG GAGAGGGACG CGGCCGCGAC 1081 GCACGAAGGC CATTG //
Primer: pCA-bgter Sequence file: RDB08526_A8Etc.seq
>08526_08526_A8Et_pCA-bgter_H04_22_ABI24.ab1     1 AAAATGGGGG TCCCCATAAA TTTTTTGGCA GAGGGAAAAG ATCGAACCCC CCTCGGAACC    61 CGTCGGGCCG CGTCGGACCG GCGGTGTTGG TCGGCGTCGG TCAGTCCTGC TCCTCGGCCA   121 CGAAGTGCAC GCAGTTGCCG GCCGGGTCGC GCAGGGCGAA CTCCCGCCCC CACGGCTGCT   181 CGCCGATCTC GGTCATGGCC GGCCCGGAGG CGTCCCGGAA GTTCGTGGAC ACGACCTCCG   241 ACCACTCGGC GTACAGCTCG TCCAGGCCGC GCACCCACAC CCAGGCCAGG GTGTTGTCCG   301 GCACCACCTG GTCCTGGACC GCGCTGATGA ACAGGGTCAC GTCGTCCCGG ACCACACCGG   361 CGAAGTCGTC CTCCACGAAG TCCCGGGAGA ACCCGAGCCG GTCGGTCCAG AACTCGACCG   421 CTCCGGCGAC GTCGCGCGCG GTGAGCACCG GAACGGCACT GGTCAACTTG GCCATGGTTG   481 TGGCCATATT ATCATCGTGT TTTTCAAAGG AAAACCACGT CCCCGTGGTT CGGGGGGCCT   541 AGACGTTTTT TTAACCTCGA CTAAACACAT GTAAAGCATG TGCACCGAGG CCCCAGATCA   601 GATCCCATAC AATGGGGTAC CTTCTGGGCA TCCTTCAGCC CCTTGTTGAA ATACGCTTGA   661 GGAGAGCCAT TTGACTCTTT CCACAACTAT CCAACTCACA ACGTGGCACT GGGGTTGTGC   721 CGCCTTTGCA GGTGTATCTT ATACACGTGG CTTTTGGCCG CAGAGGCACC TGTCGCCAGG   781 TGGGGGGGTT CCGCTGCCTG CAAAGGGTCG CTACAGACGT TGTTTGTCTT TCAGAAGCTT   841 TCCAGAGGAA CTGCTTCCTT CACGACATTC AACAGACCTT TGCATTCCTT TGGCGAGAGG   901 GGAAAGACCC CCTAAGGAAA TGCCTCGTCA AAGAAGAACA GGGCCCAAGG TTTTCCGGTC   961 CTCACATGCC CAAAAGAACG CATATGGTGA AAATAACATA ATAGGATAGC GGCACACGCT 1021 TATTCCAAGC GCTCGTCAGT TAACGTTAAG GGGAAAGGGG AAGAGG //
Primer: SV40pA3'element Reverse Sequence file: RDB08526_A8Etd.seq
>08526_08526_A8Et_SV40pA3'ElementReverse_A05_02_ABI24.ab1     1 GTGCTTGGGG GGCGGACCTA TGGAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC    61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC   121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC   181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT   241 TGGCCGATTC ATTAATGCAG CGGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA   301 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC   361 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAGC TTTTTGCAAA AGCCTAGGCC   421 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC   481 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT   541 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCGG   601 ATCCGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT   661 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG   721 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC   781 GCCAAGCTTG GGCTGCAGGT CGAGGGATCT CCATAAGAGA AGAGGGACAG CTATGACTGG   841 GAGTAGTCAG GAGAGGAGGA AAAATCTGGC TAGTAAAACA TGTAAGGAAA ATTTTAGGGA   901 TGTTAAAGAA AAAAAAATAA CACAAAAACA AAATATAAAA AAAATCTAAA CCCTCAAAGT   961 CAAAGGCTTT TCTATGGGAA ATAAAGAAAT GGGACAGCAC GGGGGCTGTT TTCATATACT  1021 GATGGACCCT CTTTATAGCC CAACCCTTTG TTTCATTGGC AGGTCAGGCA TATGGGGTCA 1081 TATGGTGCTA ACTTCTAAAC CAAAATACTC CATTTCCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl