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pCAGGS-HA-Dec1 (mouse) (#RDB08473)

Expression vector of mouse DEC1 cDNA

Clone info. Expression vector of mouse DEC1 cDNA.
Comment Tagged with HA.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.5 kb
Selectable markers Amp^r
Growth conditions 37C, LB + Amp
Gene/insert name Mouse DEC1 cDNA
Depositor|Developer Goda, Nobuhito |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(J. Hepatol. 56(2), 441-447, 2012).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって譲渡者の指定する文献を引用する(J. Hepatol. 56(2), 441-447, 2012)。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08473 pCAGGS-HA-Dec1 (mouse) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS-HA-Dec1 (mouse) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08473).

Reference section:

Nishiyama, Y., Goda, N., Kanai, M., Niwa, D., Osanai, K., Yamamoto, Y., Senoo-Matsuda, N., Johnson, R.S., Miura, S., Kabe, Y., Suematsu, M., HIF-1alpha induction suppresses excessive lipid accumulation in alcoholic fatty liver in mice. J.Hepatol. 56(2), 441-447, (2012). PMID 21896344. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08473_A6D7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB08473_A6D7a.seq check
>08473_08473_A6D7_pAxCA_F1_G10_19.ab1
    1 CTTAGTACGT CTGCTCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCA CTAGTGATTA TGTACCCATA
  121 CGATGTTCCA GATTACGCTA TGGAACGGAT CCCCAGCGCG CAACCACCTC CTACCTGCCT
  181 GCCCAAAGCT CCAGGGCTGG AGCACGGAGA CCTGTCAGGG ATGGATTTTG CCCACATGTA
  241 CCAAGTGTAC AAGTCCAGGC GGGGAATAAA ACGGAGCGAA GACAGCAAGG AAACTTACAA
  301 ACTGCCGCAC CGGCTGATTG AGAAAAAGAG ACGTGACCGG ATTAACGAGT GCATTGCCCA
  361 GCTGAAGGAT CTCCTACCCG AACATCTCAA ACTTACTACT TTGGGTCACT TGGAAAAAGC
  421 AGTGGTTCTG GAGCTTACGT TGAAGCACGT GAAAGCATTG ACAAATCTAA TTGATCAGCA
  481 GCAGCAGAAA ATCATTGCCC TGCAGAGCGG TTTACAAGCT GGTGATTTGT CGGGAAGAAA
  541 TCTCGAGGCA GGGCAAGAAA TGTTCTGCTC AGGTTTCCAG ACTTGTGCCC GTGAGGTACT
  601 TCAGTACCTG GCGAAGCATG AGAACACTCG GGACCTGAAA TCTTCCCAGC TCGTCACTCA
  661 TCTCCATCGT GTGGTCTCGG AGCTGCTGCA GGGTGGTGCT TCCAGGAAAC CATTGGACTC
  721 GGCTCCCAAA GCCGTGGACT TGAAAGAGAA GCCCAGCTTC CTAGCCAAGG GATCAGAAGG
  781 GCCCTGGGAA AAACTGTGTG CCAGTCATCC AGCGGACTTT TGGCTCCCTC GGGTGGGGGA
841 GCAG
//
Primer: pAxCA_R1
Sequence file: RDB08473_A6D7b.seq check
>08473_08473_A6D7_pAxCA_R1_H10_22.ab1
    1 TGACATGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG
   61 AATTCGATTT TAGTCTTTGG TTTCTAAGTT TAAAGGAGGG ATCTGCTTCA AAGCCTGGAG
  121 CAAGGCCGAA GAGTCGAGGG ACGAATGTGC CAAAGGAGAA GGGAGTCTCT GGGGCAGAAG
  181 CAAGGGAGTC GGTATCTTGT CTGGGTTCAT GAAGCTGGAG AGGGCTGCAG CTGAGGTGTT
  241 GAGGCCTGGG TATAACACTG GCACAGAGGT GGGGTACCAG CATTTCTCCA GCATAGGCAG
  301 GTAGGCAGTG GCCGATGGTG GGATGAGATA GAAGGGAAGG CAAAAAGGAG GCTGATGTGG
  361 GTGTGGCCCA AGAAATGGGG AACCCATCAG ATCACTGCCC GCGAAGTGGC CTTCCTCTTC
  421 TGAGAGCTGC ATTCGGCTCT TTTTGGTGGG GGGCTCTTCG GATTCTTGCT TAATTGTGCT
  481 GACACGTTCT CCCACGGCGA ACCTGCGTCC ATGGTCGCTT TTGAAGTACG GCTGCTCACT
  541 GCGCAAGTCC CCCTTCTCCA ATTCACCTCC ATAGCCACTG TCTGTGTCCG TGTCACTGCC
  601 GCTCTGCTCC CCACCCGAGG GAGCAAAAGT CCGCTGGATG ACTGGCACAC AGTTTTTCCC
  661 AGGGCCTTCT GATCCCTTGG CTAGGAAGCT GGGCTTCTCT TTCAAGTCCA CGGCTTTGGG
  721 AGCCGAGTCC AATGGTTTCC TGGAAGCACC ACCCTGCAGC AGCTCCGAGA CCACACGATG
  781 GAGATGAGTG ACGAGCTGGG AAGATTTCAG GTCCCGAGTG TTCTCATGCT TCGCCAGGTA
  841 CTGAAGTACC TCACGGGCAC AAGTCTGGAA ACCTGAGCAG AACATTTCTT GCCCTGCCTC
  901 GAGATTTCTT CCCGACAAAT CACCAGCTTG TAAACCGCTC TGCAGGGCAA TGATTTTCTG
  961 CTGCTGCTGA TCAATTAGAT TTGTCAATGC TTTCACGTGC TTCAACGTAA GCTCCCAGAA
 1021 CCACTGCTTT TTTCCCAGGT GACCCAAAGT AGTAAGTTTT GAGATGTTCG GGTAGAGATC
 1081 CCTTCAGCTG GCAATGCACT TCATAATCGG TCACGGTTCT TCATTTTCCT CAATTCCAAG
1141 C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishiyama, Y., HIF-1alpha induction suppresses excessive lipid accumulation in alcoholic fatty liver in mice. J.Hepatol. 56(2), 441-447, (2012). PMID 21896344. [link to RRC of NBRP]
user_report Tsuyama, T., Hypoxia causes pancreatic β-cell dysfunction and impairs insulin secretion by activating the transcriptional repressor BHLHE40. EMBO Rep. 24 (8): e56227 (2023). PMID 37341148. [link to RRC of NBRP]