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pCAGGS-HA-Dec1 (mouse)

Expression vector of mouse DEC1 cDNA

Catalog number RDB08473
Resource name pCAGGS-HA-Dec1 (mouse)
Clone info. Expression vector of mouse DEC1 cDNA.
Tagged with HA.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.5 kb
Selectable markers Amp^r
Gene/insert name Mouse DEC1 cDNA
Depositor Goda, Nobuhito |
 
Other clones in our bank mouse Bhlhe40 (NCBI Gene 20893) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(J. Hepatol. 56(2), 441-447, 2012).
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RDB08473 pCAGGS-HA-Dec1 (mouse) DNA solution

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References and tips

Electronic file

Featured content

References

original Nishiyama, Y., HIF-1alpha induction suppresses excessive lipid accumulation in alcoholic fatty liver in mice. J.Hepatol. 56(2), 441-447, (2012). PMID 21896344 .

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08473_A6D7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB08473_A6D7a.seq
>08473_08473_A6D7_pAxCA_F1_G10_19.ab1
    1 CTTAGTACGT CTGCTCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCA CTAGTGATTA TGTACCCATA
  121 CGATGTTCCA GATTACGCTA TGGAACGGAT CCCCAGCGCG CAACCACCTC CTACCTGCCT
  181 GCCCAAAGCT CCAGGGCTGG AGCACGGAGA CCTGTCAGGG ATGGATTTTG CCCACATGTA
  241 CCAAGTGTAC AAGTCCAGGC GGGGAATAAA ACGGAGCGAA GACAGCAAGG AAACTTACAA
  301 ACTGCCGCAC CGGCTGATTG AGAAAAAGAG ACGTGACCGG ATTAACGAGT GCATTGCCCA
  361 GCTGAAGGAT CTCCTACCCG AACATCTCAA ACTTACTACT TTGGGTCACT TGGAAAAAGC
  421 AGTGGTTCTG GAGCTTACGT TGAAGCACGT GAAAGCATTG ACAAATCTAA TTGATCAGCA
  481 GCAGCAGAAA ATCATTGCCC TGCAGAGCGG TTTACAAGCT GGTGATTTGT CGGGAAGAAA
  541 TCTCGAGGCA GGGCAAGAAA TGTTCTGCTC AGGTTTCCAG ACTTGTGCCC GTGAGGTACT
  601 TCAGTACCTG GCGAAGCATG AGAACACTCG GGACCTGAAA TCTTCCCAGC TCGTCACTCA
  661 TCTCCATCGT GTGGTCTCGG AGCTGCTGCA GGGTGGTGCT TCCAGGAAAC CATTGGACTC
  721 GGCTCCCAAA GCCGTGGACT TGAAAGAGAA GCCCAGCTTC CTAGCCAAGG GATCAGAAGG
  781 GCCCTGGGAA AAACTGTGTG CCAGTCATCC AGCGGACTTT TGGCTCCCTC GGGTGGGGGA
841 GCAG
//
Primer: pAxCA_R1 RDB08473_A6D7b.seq
>08473_08473_A6D7_pAxCA_R1_H10_22.ab1
    1 TGACATGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG
   61 AATTCGATTT TAGTCTTTGG TTTCTAAGTT TAAAGGAGGG ATCTGCTTCA AAGCCTGGAG
  121 CAAGGCCGAA GAGTCGAGGG ACGAATGTGC CAAAGGAGAA GGGAGTCTCT GGGGCAGAAG
  181 CAAGGGAGTC GGTATCTTGT CTGGGTTCAT GAAGCTGGAG AGGGCTGCAG CTGAGGTGTT
  241 GAGGCCTGGG TATAACACTG GCACAGAGGT GGGGTACCAG CATTTCTCCA GCATAGGCAG
  301 GTAGGCAGTG GCCGATGGTG GGATGAGATA GAAGGGAAGG CAAAAAGGAG GCTGATGTGG
  361 GTGTGGCCCA AGAAATGGGG AACCCATCAG ATCACTGCCC GCGAAGTGGC CTTCCTCTTC
  421 TGAGAGCTGC ATTCGGCTCT TTTTGGTGGG GGGCTCTTCG GATTCTTGCT TAATTGTGCT
  481 GACACGTTCT CCCACGGCGA ACCTGCGTCC ATGGTCGCTT TTGAAGTACG GCTGCTCACT
  541 GCGCAAGTCC CCCTTCTCCA ATTCACCTCC ATAGCCACTG TCTGTGTCCG TGTCACTGCC
  601 GCTCTGCTCC CCACCCGAGG GAGCAAAAGT CCGCTGGATG ACTGGCACAC AGTTTTTCCC
  661 AGGGCCTTCT GATCCCTTGG CTAGGAAGCT GGGCTTCTCT TTCAAGTCCA CGGCTTTGGG
  721 AGCCGAGTCC AATGGTTTCC TGGAAGCACC ACCCTGCAGC AGCTCCGAGA CCACACGATG
  781 GAGATGAGTG ACGAGCTGGG AAGATTTCAG GTCCCGAGTG TTCTCATGCT TCGCCAGGTA
  841 CTGAAGTACC TCACGGGCAC AAGTCTGGAA ACCTGAGCAG AACATTTCTT GCCCTGCCTC
  901 GAGATTTCTT CCCGACAAAT CACCAGCTTG TAAACCGCTC TGCAGGGCAA TGATTTTCTG
  961 CTGCTGCTGA TCAATTAGAT TTGTCAATGC TTTCACGTGC TTCAACGTAA GCTCCCAGAA
 1021 CCACTGCTTT TTTCCCAGGT GACCCAAAGT AGTAAGTTTT GAGATGTTCG GGTAGAGATC
 1081 CCTTCAGCTG GCAATGCACT TCATAATCGG TCACGGTTCT TCATTTTCCT CAATTCCAAG
1141 C
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nishiyama, Y., HIF-1alpha induction suppresses excessive lipid accumulation in alcoholic fatty liver in mice. J.Hepatol. 56(2), 441-447, (2012). PMID 21896344 .

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl