RDB08473 - pCAGGS-HA-Dec1 (mouse)

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCAGGS-HA-Dec1 (mouse) (#RDB08473)

Expression vector of mouse DEC1 cDNA

Clone info.	Expression vector of mouse DEC1 cDNA.
Comment	Tagged with HA.
Vector backbone	pCAGGS (Plasmid)
Size of vector backbone	4.5 kb
Selectable markers	Amp^r
Growth conditions	37C, LB + Amp
Gene/insert name	Mouse DEC1 cDNA
Depositor\|Developer	Goda, Nobuhito \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(J. Hepatol. 56(2), 441-447, 2012).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(J. Hepatol. 56(2), 441-447, 2012). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって譲渡者の指定する文献を引用する(J. Hepatol. 56(2), 441-447, 2012)。付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって譲渡者の指定する文献を引用する(J. Hepatol. 56(2), 441-447, 2012)。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08473	pCAGGS-HA-Dec1 (mouse)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS-HA-Dec1 (mouse) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08473).

Reference section:

Nishiyama, Y., Goda, N., Kanai, M., Niwa, D., Osanai, K., Yamamoto, Y., Senoo-Matsuda, N., Johnson, R.S., Miura, S., Kabe, Y., Suematsu, M., HIF-1alpha induction suppresses excessive lipid accumulation in alcoholic fatty liver in mice. J.Hepatol. 56(2), 441-447, (2012). PMID 21896344. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08473_A6D7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB08473_A6D7a.seq
>08473_08473_A6D7_pAxCA_F1_G10_19.ab1 1 CTTAGTACGT CTGCTCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG 61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCA CTAGTGATTA TGTACCCATA 121 CGATGTTCCA GATTACGCTA TGGAACGGAT CCCCAGCGCG CAACCACCTC CTACCTGCCT 181 GCCCAAAGCT CCAGGGCTGG AGCACGGAGA CCTGTCAGGG ATGGATTTTG CCCACATGTA 241 CCAAGTGTAC AAGTCCAGGC GGGGAATAAA ACGGAGCGAA GACAGCAAGG AAACTTACAA 301 ACTGCCGCAC CGGCTGATTG AGAAAAAGAG ACGTGACCGG ATTAACGAGT GCATTGCCCA 361 GCTGAAGGAT CTCCTACCCG AACATCTCAA ACTTACTACT TTGGGTCACT TGGAAAAAGC 421 AGTGGTTCTG GAGCTTACGT TGAAGCACGT GAAAGCATTG ACAAATCTAA TTGATCAGCA 481 GCAGCAGAAA ATCATTGCCC TGCAGAGCGG TTTACAAGCT GGTGATTTGT CGGGAAGAAA 541 TCTCGAGGCA GGGCAAGAAA TGTTCTGCTC AGGTTTCCAG ACTTGTGCCC GTGAGGTACT 601 TCAGTACCTG GCGAAGCATG AGAACACTCG GGACCTGAAA TCTTCCCAGC TCGTCACTCA 661 TCTCCATCGT GTGGTCTCGG AGCTGCTGCA GGGTGGTGCT TCCAGGAAAC CATTGGACTC 721 GGCTCCCAAA GCCGTGGACT TGAAAGAGAA GCCCAGCTTC CTAGCCAAGG GATCAGAAGG 781 GCCCTGGGAA AAACTGTGTG CCAGTCATCC AGCGGACTTT TGGCTCCCTC GGGTGGGGGA 841 GCAG //
Primer: pAxCA_R1 Sequence file: RDB08473_A6D7b.seq
>08473_08473_A6D7_pAxCA_R1_H10_22.ab1 1 TGACATGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG 61 AATTCGATTT TAGTCTTTGG TTTCTAAGTT TAAAGGAGGG ATCTGCTTCA AAGCCTGGAG 121 CAAGGCCGAA GAGTCGAGGG ACGAATGTGC CAAAGGAGAA GGGAGTCTCT GGGGCAGAAG 181 CAAGGGAGTC GGTATCTTGT CTGGGTTCAT GAAGCTGGAG AGGGCTGCAG CTGAGGTGTT 241 GAGGCCTGGG TATAACACTG GCACAGAGGT GGGGTACCAG CATTTCTCCA GCATAGGCAG 301 GTAGGCAGTG GCCGATGGTG GGATGAGATA GAAGGGAAGG CAAAAAGGAG GCTGATGTGG 361 GTGTGGCCCA AGAAATGGGG AACCCATCAG ATCACTGCCC GCGAAGTGGC CTTCCTCTTC 421 TGAGAGCTGC ATTCGGCTCT TTTTGGTGGG GGGCTCTTCG GATTCTTGCT TAATTGTGCT 481 GACACGTTCT CCCACGGCGA ACCTGCGTCC ATGGTCGCTT TTGAAGTACG GCTGCTCACT 541 GCGCAAGTCC CCCTTCTCCA ATTCACCTCC ATAGCCACTG TCTGTGTCCG TGTCACTGCC 601 GCTCTGCTCC CCACCCGAGG GAGCAAAAGT CCGCTGGATG ACTGGCACAC AGTTTTTCCC 661 AGGGCCTTCT GATCCCTTGG CTAGGAAGCT GGGCTTCTCT TTCAAGTCCA CGGCTTTGGG 721 AGCCGAGTCC AATGGTTTCC TGGAAGCACC ACCCTGCAGC AGCTCCGAGA CCACACGATG 781 GAGATGAGTG ACGAGCTGGG AAGATTTCAG GTCCCGAGTG TTCTCATGCT TCGCCAGGTA 841 CTGAAGTACC TCACGGGCAC AAGTCTGGAA ACCTGAGCAG AACATTTCTT GCCCTGCCTC 901 GAGATTTCTT CCCGACAAAT CACCAGCTTG TAAACCGCTC TGCAGGGCAA TGATTTTCTG 961 CTGCTGCTGA TCAATTAGAT TTGTCAATGC TTTCACGTGC TTCAACGTAA GCTCCCAGAA 1021 CCACTGCTTT TTTCCCAGGT GACCCAAAGT AGTAAGTTTT GAGATGTTCG GGTAGAGATC 1081 CCTTCAGCTG GCAATGCACT TCATAATCGG TCACGGTTCT TCATTTTCCT CAATTCCAAG 1141 C //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nishiyama, Y., HIF-1alpha induction suppresses excessive lipid accumulation in alcoholic fatty liver in mice. J.Hepatol. 56(2), 441-447, (2012). PMID 21896344. [link to RRC of NBRP]
user_report	Tsuyama, T., Hypoxia causes pancreatic β-cell dysfunction and impairs insulin secretion by activating the transcriptional repressor BHLHE40. EMBO Rep. 24 (8): e56227 (2023). PMID 37341148. [link to RRC of NBRP]

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Other resources in our bank

mouse DEC1

External Database
Mouse DEC1

Reference sequence