Sequencing and PCR primers for primer information.
Expression vector of beta-D-glucoside glucohydrolase 1 of Trichoderma reesei
| Catalog number | RDB08311 |
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| Resource name | pET25b_Tr-bgl1-His |
| Alternative name | pET25b_Tr008-His |
| Clone info. | Biomass collection. the beta-D-glucoside glucohydrolase 1 (bgl 1) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b. |
| Vector backbone | pET-25b (Plasmid) |
| Size of vector backbone | 5,547 bp |
| Selectable markers | Amp^r |
| Gene/insert name | Trichoderma reesei bgl1 cDNA |
| Depositor | DNA Bank, | Kishikawa, Shotaro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08311 | pET25b_Tr-bgl1-His | DNA solution |
Electronic file
| Electronic file | Sequence RDB08311z.seq from Depositor |
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| Electronic file | PDF RDB08311.pdf from Depositor |
Featured content
| Featured content | Biomass Collection (English text) |
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| Featured content | Biomass Collection (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08311_A5Iip1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_20bp | RDB08311_A5Iia.seq |
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>08311_08311_A5Ii_T7_20bp_E08_14.ab1 1 TGCGTCGTTC CCTCTAGAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGAAAT 61 ACCTGCTGCC GACCGCTGCT GCTGGTCTGC TGCTCCTCGC TGCCCAGCCG GCGATGGCCA 121 TGGATATCGG AATTAATTCG GATCCGAATT CGAGCTCCGT CGACGACAGT AGCTCAACAT 181 CGGGGGCCTC GGCTGAGGCA GTTGTACCTC CTGCAGGGAC TCCATGGGGA ACCGCGTACG 241 ACAAGGCGAA GGCCGCATTG GCAAAGCTCA ATCTCCAAGA TAAGGTCGGC ATCGTGAGCG 301 GTGTCGGCTG GAACGGCGGT CCTTGCGTTG GAAACACATC TCCGGCCTCC AAGATCAGCT 361 ATCCATCGCT ATGCCTTCAA GACGGACCCC TCGGTGTTCG ATACTCGACA GGCAGCACAG 421 CCTTTACGCC GGGCGTTCAA GCGGCCTCGA CGTGGGATGT CAATTTGATC CGCGAACGTG 481 GACAGTTCAT CGGTGAGGAG GTGAAGGCCT CGGGGATTCA TGTCATACTT GGTCCTGTGG 541 CTGGGCCGCT GGGAAAGACT CCGCAGGGCG GTCGCAACTG GGAGGGCTTC GGTGTCGATC 601 CATATCTCAC GGGCATTGCC ATGGGTCAAA CCATCAACGG CATCCAGTCG GTAGGCGTGC 661 AGGCGACAGC GAAGCACTAT ATCCTCAACG AGCAGGAGCT CAATCGAGAA ACCATTTCGA 721 GCAACCCAGA TGACCGAACT CTCCATGAGC TGTATACTTG GCCATTTGCC GACGCGGTTC 781 AGGCCAATGT CGCTTCTGTC ATGTGCTCGT ACAACAAGGT CAATACCACC TGGGCCTGCG 841 AGGATCAGTA CACGCTGCAG ACTGTGCTGA AAGACCAGCT GGGGTTCCCA GGCTATGTCA 901 TGACGGACTG GAACGCACAG CACACGACTG TCCAAAGCGC GAATTCTGGG CTTGACATGT 961 CAATGCCTGG CACAGACTTC AACGGTAACA ATCGGCTCTG GGGGTCCAGC TCTCACCCAA 1021 TGCGGGTAAA TAGCAAATCA TGTCCCCCAC GAGCAGAGTC GACGGATATG GTGACTCGTA 1081 TCCTCGCCGC AATGTACTGA CAGCCAGGAC CAGGCCAGGC TATATCCCGG TTCGGTTTCC // |
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| Primer: f1ori_R3 | RDB08311_A5Iib.seq |
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>08311_08311_A5Ii_f1ori_R3_F08_17.ab1 1 GCATCCATCG GATTAGTTCC TCCTTTCAGC AAAAACCCCT CAAGACCCGT TTAGAGGCCC 61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG 121 CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG CTCGACATCC TCGGGGTCTT 181 CCGGGGCGAG TTCTGGCTGG CTAGCCCGTT TGATCTCGAG TGCGGCCGCA AGCTTGTCGA 241 CCGCTACCGA CAGAGTGCTC GTCAGCCTGA TATCCCGGCT GCTCGCTCCC ACGCTGATGC 301 CAAACGACCC CGACGGCACC ACCCATTTCT GCGAAGCCGT GTCCCAGTAG CTGAGATCTC 361 GTCGTCGGAT GTTGAACGTT GCTGTTCCGC TCTGACCAGG CGTGAGGTTC AGCTTGGCAA 421 AGCCTCGCAG CTGCTTCGGA GGGGTCCTGG GTGCTGAAGA TGGGTAGGTG ATGTACAGCT 481 GGGCTACCTC GGCACCAGTC ACTTGGCCAG AGTTTGCGAT GTCAACGGTG ACTGTCGCGA 541 CATTCTGGAA CAGATCACTC GGGCCTCCCG GCACAACGGC CCCAGTCGCA GGACCAGACT 601 TGGCGGTCGA CAAGACGGAG AGGCGTGAGT AGTTGAACTT GGTGTAAGAC AGTCCATAGC 661 CGAACTCGTA CCGCGGCGTG ATATTGGCGT CGTCGAAGTG CTTATAGTCG ATGAACAGTC 721 CCTCGCTGAA GCTGTCACTG CCGCCGGAAA CGATGCGAGT GTTATAGTCA TTGGGGCTCT 781 TCGCAATGGT GTACACCAGC TTGCCAGAAG GGCTGACATC TCCCCACAGC ACGTCGACGA 841 GCGCATTGCC GCTCTCCTGA GAAGGAAGAC CCGCCCAGAC AACGGCCTTG ACCTGCGGAA 901 GAGCAAGAAT CTGCTCCAGA ATGATGGCGC CAACGGAGTG GACAACAACA ATGACGTTGC 961 TGTTGGCACC GGCCACCGCC TGGACCAGGG CATTGCCGTT GTGCCACGGG ATCCAGGGTT 1021 GTTGCGATCG CCCGCGTTTG CCCTCCACGG TGATGTAGCC CTTTCACCCC GAGTCGGCGT 1081 GATGAGACGA TGGGCGAACG TCCTTCCTCT TGCCTGCAGG ATGCGCCTGA GGGACGTGTT 1141 TGATCAGGGT GGATC // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl