RDB08311 - pET25b_Tr-bgl1-His

Resource data sheet
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pET25b_Tr-bgl1-His

Expression vector of beta-D-glucoside glucohydrolase 1 of Trichoderma reesei

Catalog number	RDB08311
Resource name	pET25b_Tr-bgl1-His
Alternative name	pET25b_Tr008-His
Clone info.	Biomass collection. the beta-D-glucoside glucohydrolase 1 (bgl 1) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone	pET-25b (Plasmid)
Size of vector backbone	5,547 bp
Selectable markers	Amp^r
Gene/insert name	Trichoderma reesei bgl1 cDNA
Depositor\|Developer	DNA Bank, \| Kishikawa, Shotaro \|

Other clones in our bank

External Database
Trichoderma reesei bgl1

Reference sequence

Sequence	RDB08311z.seq
Remarks, protocol and/or map (pdf)	RDB08311.pdf

Distribution information

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RDB08311	pET25b_Tr-bgl1-His	DNA solution

Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The pET25b_Tr-bgl1-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08311).

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	Biomass Collection (English text)
Featured content	Biomass Collection (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08311_A5Iip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB08311_A5Iia.seq
>08311_08311_A5Ii_T7_20bp_E08_14.ab1 1 TGCGTCGTTC CCTCTAGAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGAAAT 61 ACCTGCTGCC GACCGCTGCT GCTGGTCTGC TGCTCCTCGC TGCCCAGCCG GCGATGGCCA 121 TGGATATCGG AATTAATTCG GATCCGAATT CGAGCTCCGT CGACGACAGT AGCTCAACAT 181 CGGGGGCCTC GGCTGAGGCA GTTGTACCTC CTGCAGGGAC TCCATGGGGA ACCGCGTACG 241 ACAAGGCGAA GGCCGCATTG GCAAAGCTCA ATCTCCAAGA TAAGGTCGGC ATCGTGAGCG 301 GTGTCGGCTG GAACGGCGGT CCTTGCGTTG GAAACACATC TCCGGCCTCC AAGATCAGCT 361 ATCCATCGCT ATGCCTTCAA GACGGACCCC TCGGTGTTCG ATACTCGACA GGCAGCACAG 421 CCTTTACGCC GGGCGTTCAA GCGGCCTCGA CGTGGGATGT CAATTTGATC CGCGAACGTG 481 GACAGTTCAT CGGTGAGGAG GTGAAGGCCT CGGGGATTCA TGTCATACTT GGTCCTGTGG 541 CTGGGCCGCT GGGAAAGACT CCGCAGGGCG GTCGCAACTG GGAGGGCTTC GGTGTCGATC 601 CATATCTCAC GGGCATTGCC ATGGGTCAAA CCATCAACGG CATCCAGTCG GTAGGCGTGC 661 AGGCGACAGC GAAGCACTAT ATCCTCAACG AGCAGGAGCT CAATCGAGAA ACCATTTCGA 721 GCAACCCAGA TGACCGAACT CTCCATGAGC TGTATACTTG GCCATTTGCC GACGCGGTTC 781 AGGCCAATGT CGCTTCTGTC ATGTGCTCGT ACAACAAGGT CAATACCACC TGGGCCTGCG 841 AGGATCAGTA CACGCTGCAG ACTGTGCTGA AAGACCAGCT GGGGTTCCCA GGCTATGTCA 901 TGACGGACTG GAACGCACAG CACACGACTG TCCAAAGCGC GAATTCTGGG CTTGACATGT 961 CAATGCCTGG CACAGACTTC AACGGTAACA ATCGGCTCTG GGGGTCCAGC TCTCACCCAA 1021 TGCGGGTAAA TAGCAAATCA TGTCCCCCAC GAGCAGAGTC GACGGATATG GTGACTCGTA 1081 TCCTCGCCGC AATGTACTGA CAGCCAGGAC CAGGCCAGGC TATATCCCGG TTCGGTTTCC //
Primer: f1ori_R3 Sequence file: RDB08311_A5Iib.seq
>08311_08311_A5Ii_f1ori_R3_F08_17.ab1 1 GCATCCATCG GATTAGTTCC TCCTTTCAGC AAAAACCCCT CAAGACCCGT TTAGAGGCCC 61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG 121 CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG CTCGACATCC TCGGGGTCTT 181 CCGGGGCGAG TTCTGGCTGG CTAGCCCGTT TGATCTCGAG TGCGGCCGCA AGCTTGTCGA 241 CCGCTACCGA CAGAGTGCTC GTCAGCCTGA TATCCCGGCT GCTCGCTCCC ACGCTGATGC 301 CAAACGACCC CGACGGCACC ACCCATTTCT GCGAAGCCGT GTCCCAGTAG CTGAGATCTC 361 GTCGTCGGAT GTTGAACGTT GCTGTTCCGC TCTGACCAGG CGTGAGGTTC AGCTTGGCAA 421 AGCCTCGCAG CTGCTTCGGA GGGGTCCTGG GTGCTGAAGA TGGGTAGGTG ATGTACAGCT 481 GGGCTACCTC GGCACCAGTC ACTTGGCCAG AGTTTGCGAT GTCAACGGTG ACTGTCGCGA 541 CATTCTGGAA CAGATCACTC GGGCCTCCCG GCACAACGGC CCCAGTCGCA GGACCAGACT 601 TGGCGGTCGA CAAGACGGAG AGGCGTGAGT AGTTGAACTT GGTGTAAGAC AGTCCATAGC 661 CGAACTCGTA CCGCGGCGTG ATATTGGCGT CGTCGAAGTG CTTATAGTCG ATGAACAGTC 721 CCTCGCTGAA GCTGTCACTG CCGCCGGAAA CGATGCGAGT GTTATAGTCA TTGGGGCTCT 781 TCGCAATGGT GTACACCAGC TTGCCAGAAG GGCTGACATC TCCCCACAGC ACGTCGACGA 841 GCGCATTGCC GCTCTCCTGA GAAGGAAGAC CCGCCCAGAC AACGGCCTTG ACCTGCGGAA 901 GAGCAAGAAT CTGCTCCAGA ATGATGGCGC CAACGGAGTG GACAACAACA ATGACGTTGC 961 TGTTGGCACC GGCCACCGCC TGGACCAGGG CATTGCCGTT GTGCCACGGG ATCCAGGGTT 1021 GTTGCGATCG CCCGCGTTTG CCCTCCACGG TGATGTAGCC CTTTCACCCC GAGTCGGCGT 1081 GATGAGACGA TGGGCGAACG TCCTTCCTCT TGCCTGCAGG ATGCGCCTGA GGGACGTGTT 1141 TGATCAGGGT GGATC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl