Resource data sheet
Prev.

pET25b_Tr-bgl1-His

Expression vector of beta-D-glucoside glucohydrolase 1 of Trichoderma reesei

Catalog number RDB08311
Resource name pET25b_Tr-bgl1-His
Alternative name pET25b_Tr008-His
Clone info. Biomass collection. the beta-D-glucoside glucohydrolase 1 (bgl 1) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone pET-25b (Plasmid)
Size of vector backbone 5,547 bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei bgl1 cDNA
Depositor DNA Bank, | Kishikawa, Shotaro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08311 pET25b_Tr-bgl1-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08311z.seq from Depositor
Electronic file PDF RDB08311.pdf from Depositor

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08311_A5Iip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08311_A5Iia.seq
>08311_08311_A5Ii_T7_20bp_E08_14.ab1
    1 TGCGTCGTTC CCTCTAGAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGAAAT
   61 ACCTGCTGCC GACCGCTGCT GCTGGTCTGC TGCTCCTCGC TGCCCAGCCG GCGATGGCCA
  121 TGGATATCGG AATTAATTCG GATCCGAATT CGAGCTCCGT CGACGACAGT AGCTCAACAT
  181 CGGGGGCCTC GGCTGAGGCA GTTGTACCTC CTGCAGGGAC TCCATGGGGA ACCGCGTACG
  241 ACAAGGCGAA GGCCGCATTG GCAAAGCTCA ATCTCCAAGA TAAGGTCGGC ATCGTGAGCG
  301 GTGTCGGCTG GAACGGCGGT CCTTGCGTTG GAAACACATC TCCGGCCTCC AAGATCAGCT
  361 ATCCATCGCT ATGCCTTCAA GACGGACCCC TCGGTGTTCG ATACTCGACA GGCAGCACAG
  421 CCTTTACGCC GGGCGTTCAA GCGGCCTCGA CGTGGGATGT CAATTTGATC CGCGAACGTG
  481 GACAGTTCAT CGGTGAGGAG GTGAAGGCCT CGGGGATTCA TGTCATACTT GGTCCTGTGG
  541 CTGGGCCGCT GGGAAAGACT CCGCAGGGCG GTCGCAACTG GGAGGGCTTC GGTGTCGATC
  601 CATATCTCAC GGGCATTGCC ATGGGTCAAA CCATCAACGG CATCCAGTCG GTAGGCGTGC
  661 AGGCGACAGC GAAGCACTAT ATCCTCAACG AGCAGGAGCT CAATCGAGAA ACCATTTCGA
  721 GCAACCCAGA TGACCGAACT CTCCATGAGC TGTATACTTG GCCATTTGCC GACGCGGTTC
  781 AGGCCAATGT CGCTTCTGTC ATGTGCTCGT ACAACAAGGT CAATACCACC TGGGCCTGCG
  841 AGGATCAGTA CACGCTGCAG ACTGTGCTGA AAGACCAGCT GGGGTTCCCA GGCTATGTCA
  901 TGACGGACTG GAACGCACAG CACACGACTG TCCAAAGCGC GAATTCTGGG CTTGACATGT
  961 CAATGCCTGG CACAGACTTC AACGGTAACA ATCGGCTCTG GGGGTCCAGC TCTCACCCAA
 1021 TGCGGGTAAA TAGCAAATCA TGTCCCCCAC GAGCAGAGTC GACGGATATG GTGACTCGTA
 1081 TCCTCGCCGC AATGTACTGA CAGCCAGGAC CAGGCCAGGC TATATCCCGG TTCGGTTTCC
//
Primer: f1ori_R3 RDB08311_A5Iib.seq
>08311_08311_A5Ii_f1ori_R3_F08_17.ab1
    1 GCATCCATCG GATTAGTTCC TCCTTTCAGC AAAAACCCCT CAAGACCCGT TTAGAGGCCC
   61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
  121 CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG CTCGACATCC TCGGGGTCTT
  181 CCGGGGCGAG TTCTGGCTGG CTAGCCCGTT TGATCTCGAG TGCGGCCGCA AGCTTGTCGA
  241 CCGCTACCGA CAGAGTGCTC GTCAGCCTGA TATCCCGGCT GCTCGCTCCC ACGCTGATGC
  301 CAAACGACCC CGACGGCACC ACCCATTTCT GCGAAGCCGT GTCCCAGTAG CTGAGATCTC
  361 GTCGTCGGAT GTTGAACGTT GCTGTTCCGC TCTGACCAGG CGTGAGGTTC AGCTTGGCAA
  421 AGCCTCGCAG CTGCTTCGGA GGGGTCCTGG GTGCTGAAGA TGGGTAGGTG ATGTACAGCT
  481 GGGCTACCTC GGCACCAGTC ACTTGGCCAG AGTTTGCGAT GTCAACGGTG ACTGTCGCGA
  541 CATTCTGGAA CAGATCACTC GGGCCTCCCG GCACAACGGC CCCAGTCGCA GGACCAGACT
  601 TGGCGGTCGA CAAGACGGAG AGGCGTGAGT AGTTGAACTT GGTGTAAGAC AGTCCATAGC
  661 CGAACTCGTA CCGCGGCGTG ATATTGGCGT CGTCGAAGTG CTTATAGTCG ATGAACAGTC
  721 CCTCGCTGAA GCTGTCACTG CCGCCGGAAA CGATGCGAGT GTTATAGTCA TTGGGGCTCT
  781 TCGCAATGGT GTACACCAGC TTGCCAGAAG GGCTGACATC TCCCCACAGC ACGTCGACGA
  841 GCGCATTGCC GCTCTCCTGA GAAGGAAGAC CCGCCCAGAC AACGGCCTTG ACCTGCGGAA
  901 GAGCAAGAAT CTGCTCCAGA ATGATGGCGC CAACGGAGTG GACAACAACA ATGACGTTGC
  961 TGTTGGCACC GGCCACCGCC TGGACCAGGG CATTGCCGTT GTGCCACGGG ATCCAGGGTT
 1021 GTTGCGATCG CCCGCGTTTG CCCTCCACGG TGATGTAGCC CTTTCACCCC GAGTCGGCGT
 1081 GATGAGACGA TGGGCGAACG TCCTTCCTCT TGCCTGCAGG ATGCGCCTGA GGGACGTGTT
1141 TGATCAGGGT GGATC
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl