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pET25b_Tr-egl1-His

Expression vector of Endoglucanase EG-I of Trichoderma reesei

Catalog number RDB08308
Resource name pET25b_Tr-egl1-His
Alternative name pET25b_Tr003-His
Clone info. Biomass collection. the Endoglucanase EG-I (EGLI, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone pET-25b (Plasmid)
Size of vector backbone 5,547 bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei egl1 cDNA
Depositor DNA Bank, | Kishikawa, Shotaro |

Distribution information

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RDB08308 pET25b_Tr-egl1-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08308z.seq from Depositor
Electronic file PDF RDB08308.pdf from Depositor

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08308_A5Iip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08308_A5Iia.seq
>08308_08308_A5Ii_T7_20bp_A01_01.ab1
    1 TGCGGGTCAT TCCCCTCTAG AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATGA
   61 AATACCTGCT GCCGACCGCT GCTGCTGGTC TGCTGCTCCT CGCTGCCCAG CCGGCGATGG
  121 CCATGGATAT CGGAATTAAT TCGGATCCGA ATTCGAGCTC CGTCGACCAG CAACCGGGTA
  181 CCAGCACCCC CGAGGTCCAT CCCAAGTTGA CAACCTACAA GTGTACAAAG TCCGGGGGGT
  241 GCGTGGCCCA GGACACCTCG GTGGTCCTTG ACTGGAACTA CCGCTGGATG CACGACGCAA
  301 ACTACAACTC GTGCACCGTC AACGGCGGCG TCAACACCAC GCTCTGCCCT GACGAGGCGA
  361 CCTGTGGCAA GAACTGCTTC ATCGAGGGCG TCGACTACGC CGCCTCGGGC GTCACGACCT
  421 CGGGCAGCAG CCTCACCATG AACCAGTACA TGCCCAGCAG CTCTGGCGGC TACAGCAGCG
  481 TCTCTCCTCG GCTGTATCTC CTGGACTCTG ACGGTGAGTA CGTGATGCTG AAGCTCAACG
  541 GCCAGGAGCT GAGCTTCGAC GTCGACCTCT CTGCTCTGCC GTGTGGAGAG AACGGCTCGC
  601 TCTACCTGTC TCAGATGGAC GAGAACGGGG GCGCCAACCA GTATAACACG GCCGGTGCCA
  661 ACTACGGGAG CGGCTACTGC GATGCTCAGT GCCCCGTCCA GACATGGAGG AACGGCACCC
  721 TCAACACTAG CCACCAGGGC TTCTGCTGCA ACGAGATGGA TATCCTGGAG GGCAACTCGA
  781 GGGCGAATGC CTTGACCCCT CACTCTTGCA CGGCCACGGC CTGCGACTCT GCCGGTTGCG
  841 GCTTCAACCC CTATGGCAGC GGCTACAAAA GCTACTACGG CCCCGGAGAT ACCGTTGACA
  901 CCTCCAAGAC CTTCACCATC ATCACCCAGT TCAACACGGA CAACGGCTCG CCCTCGGGCA
  961 ACCTTGTGAG CATCACCCGC AAGTACCAGC AAAACGGCGT CGACATCCCC AGCGCCCAGC
 1021 CCGGCGGCGA CACCATCTCG TCTGCTCGTC GTCTCAGCCT ACGGCGGCTC GCACATGGGC
 1081 AAGTCCTGAG CCAGCGCATG TGCTCGTGAT CAGCCAATTG GGAAACGGAA CAACAAGCCA
1141 GGTATAACAA TGA
//
Primer: f1ori_R3 RDB08308_A5Iib.seq
>08308_08308_A5Ii_f1ori_R3_B01_04.ab1
    1 GATTGGCATC GGATTAGTTC CTCCTTTCAG CAAAACCCCT CAAGACCCGT TTAGAGGCCC
   61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
  121 CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG CTCGACATCC TCGGGGTCTT
  181 CCGGGGCGAG TTCTGGCTGG CTAGCCCGTT TGATCTCGAG TGCGGCCGCA AGCTTGTCGA
  241 CAAGGCATTG CGAGTAGTAG TCGTTGCTAT ACTGGCACGT AGTGCCCGAC GTGCACGTCT
  301 TGCACCCGCT GTACCCAATG CCACCGCACT GCCCCCAGTG AGTCTGCGTG CAGCTCGGGC
  361 TGCTCGAAGT CGTCGAGCTC CTCCGTGTAG TCGAAAACGT CGTGCTGGAC GCAGGCGGGG
  421 GCGGGGGCGC AGTCGAGTTC GTAGTAGACC CAATGTCTCC CCAGCGGATG TTGGAGAAGA
  481 CGACGTGCGT GTTGGGGTTG TTGGCCAGGA TGTTGGATGG GTTGCCCTCG GTGCTGCTGC
  541 AGGGGCCGGC GTTGCCGCTG TCGAGCCAGT TCATGTACTG GCTGTTGTCG TTCCAAATGC
  601 TGAACACGAG CACCATGCCG CTGCTCAGGG CCTTGCCCAT GGTGGCGAGG CCGCCGTAGG
  661 CTGAGGCGGA CGGGCAGGAC GAGATGGTGT CGCCGCCGGG CTGGGCGCTG GGGATGTCGA
  721 CGCCGTTTTG CTGGTACTTG CGGGTGATGC TCACAAGGTT GCCCGAGGGC GAGCCGTTGT
  781 CCGTGTTGAA CTGGGTGATG ATGGTGAAGG TCTTGGAGGT GTCAACGGTA TCTCCGGGGC
  841 CGTAGTAGCT TTTGTAGCCG CTGCCATAGG GGTTGAAGCC GCAACCGGCA GAGTCGCAGG
  901 CCGTGGCCGT GCAAGAGTGA GGGGTCAAGG CATTCGCCCT CGAGTTGCCC TCCAGGATAT
  961 CCATCTCGTT GCAGCAGAAG CCCTGGTGGG CTAGTGTTTG AGGGTGCCGT TCCTCCATGT
 1021 CTGGACGGGG CACTGGAGCA TCGCAGTAGC CGCCTCCCGT AGTGGCACCG GGCCGTGTTA
 1081 TACTGGGTGG GCGCCCCCGA TTCTCGGTTC CAATCTGAGA ACAGTTAGAG GCGGAGTCCG
1141 ATTCTTCTTC ATCAACGGGC CAGAAGCCAG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

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