Sequencing and PCR primers for primer information.
Expression vector of Endoglucanase EG-I of Trichoderma reesei
| Catalog number | RDB08308 |
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| Resource name | pET25b_Tr-egl1-His |
| Alternative name | pET25b_Tr003-His |
| Clone info. | Biomass collection. the Endoglucanase EG-I (EGLI, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b. |
| Vector backbone | pET-25b (Plasmid) |
| Size of vector backbone | 5,547 bp |
| Selectable markers | Amp^r |
| Gene/insert name | Trichoderma reesei egl1 cDNA |
| Depositor | DNA Bank, | Kishikawa, Shotaro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08308 | pET25b_Tr-egl1-His | DNA solution |
Electronic file
| Electronic file | Sequence RDB08308z.seq from Depositor |
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| Electronic file | PDF RDB08308.pdf from Depositor |
Featured content
| Featured content | Biomass Collection (English text) |
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| Featured content | Biomass Collection (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08308_A5Iip1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_20bp | RDB08308_A5Iia.seq |
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>08308_08308_A5Ii_T7_20bp_A01_01.ab1 1 TGCGGGTCAT TCCCCTCTAG AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATGA 61 AATACCTGCT GCCGACCGCT GCTGCTGGTC TGCTGCTCCT CGCTGCCCAG CCGGCGATGG 121 CCATGGATAT CGGAATTAAT TCGGATCCGA ATTCGAGCTC CGTCGACCAG CAACCGGGTA 181 CCAGCACCCC CGAGGTCCAT CCCAAGTTGA CAACCTACAA GTGTACAAAG TCCGGGGGGT 241 GCGTGGCCCA GGACACCTCG GTGGTCCTTG ACTGGAACTA CCGCTGGATG CACGACGCAA 301 ACTACAACTC GTGCACCGTC AACGGCGGCG TCAACACCAC GCTCTGCCCT GACGAGGCGA 361 CCTGTGGCAA GAACTGCTTC ATCGAGGGCG TCGACTACGC CGCCTCGGGC GTCACGACCT 421 CGGGCAGCAG CCTCACCATG AACCAGTACA TGCCCAGCAG CTCTGGCGGC TACAGCAGCG 481 TCTCTCCTCG GCTGTATCTC CTGGACTCTG ACGGTGAGTA CGTGATGCTG AAGCTCAACG 541 GCCAGGAGCT GAGCTTCGAC GTCGACCTCT CTGCTCTGCC GTGTGGAGAG AACGGCTCGC 601 TCTACCTGTC TCAGATGGAC GAGAACGGGG GCGCCAACCA GTATAACACG GCCGGTGCCA 661 ACTACGGGAG CGGCTACTGC GATGCTCAGT GCCCCGTCCA GACATGGAGG AACGGCACCC 721 TCAACACTAG CCACCAGGGC TTCTGCTGCA ACGAGATGGA TATCCTGGAG GGCAACTCGA 781 GGGCGAATGC CTTGACCCCT CACTCTTGCA CGGCCACGGC CTGCGACTCT GCCGGTTGCG 841 GCTTCAACCC CTATGGCAGC GGCTACAAAA GCTACTACGG CCCCGGAGAT ACCGTTGACA 901 CCTCCAAGAC CTTCACCATC ATCACCCAGT TCAACACGGA CAACGGCTCG CCCTCGGGCA 961 ACCTTGTGAG CATCACCCGC AAGTACCAGC AAAACGGCGT CGACATCCCC AGCGCCCAGC 1021 CCGGCGGCGA CACCATCTCG TCTGCTCGTC GTCTCAGCCT ACGGCGGCTC GCACATGGGC 1081 AAGTCCTGAG CCAGCGCATG TGCTCGTGAT CAGCCAATTG GGAAACGGAA CAACAAGCCA 1141 GGTATAACAA TGA // |
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| Primer: f1ori_R3 | RDB08308_A5Iib.seq |
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>08308_08308_A5Ii_f1ori_R3_B01_04.ab1 1 GATTGGCATC GGATTAGTTC CTCCTTTCAG CAAAACCCCT CAAGACCCGT TTAGAGGCCC 61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG 121 CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG CTCGACATCC TCGGGGTCTT 181 CCGGGGCGAG TTCTGGCTGG CTAGCCCGTT TGATCTCGAG TGCGGCCGCA AGCTTGTCGA 241 CAAGGCATTG CGAGTAGTAG TCGTTGCTAT ACTGGCACGT AGTGCCCGAC GTGCACGTCT 301 TGCACCCGCT GTACCCAATG CCACCGCACT GCCCCCAGTG AGTCTGCGTG CAGCTCGGGC 361 TGCTCGAAGT CGTCGAGCTC CTCCGTGTAG TCGAAAACGT CGTGCTGGAC GCAGGCGGGG 421 GCGGGGGCGC AGTCGAGTTC GTAGTAGACC CAATGTCTCC CCAGCGGATG TTGGAGAAGA 481 CGACGTGCGT GTTGGGGTTG TTGGCCAGGA TGTTGGATGG GTTGCCCTCG GTGCTGCTGC 541 AGGGGCCGGC GTTGCCGCTG TCGAGCCAGT TCATGTACTG GCTGTTGTCG TTCCAAATGC 601 TGAACACGAG CACCATGCCG CTGCTCAGGG CCTTGCCCAT GGTGGCGAGG CCGCCGTAGG 661 CTGAGGCGGA CGGGCAGGAC GAGATGGTGT CGCCGCCGGG CTGGGCGCTG GGGATGTCGA 721 CGCCGTTTTG CTGGTACTTG CGGGTGATGC TCACAAGGTT GCCCGAGGGC GAGCCGTTGT 781 CCGTGTTGAA CTGGGTGATG ATGGTGAAGG TCTTGGAGGT GTCAACGGTA TCTCCGGGGC 841 CGTAGTAGCT TTTGTAGCCG CTGCCATAGG GGTTGAAGCC GCAACCGGCA GAGTCGCAGG 901 CCGTGGCCGT GCAAGAGTGA GGGGTCAAGG CATTCGCCCT CGAGTTGCCC TCCAGGATAT 961 CCATCTCGTT GCAGCAGAAG CCCTGGTGGG CTAGTGTTTG AGGGTGCCGT TCCTCCATGT 1021 CTGGACGGGG CACTGGAGCA TCGCAGTAGC CGCCTCCCGT AGTGGCACCG GGCCGTGTTA 1081 TACTGGGTGG GCGCCCCCGA TTCTCGGTTC CAATCTGAGA ACAGTTAGAG GCGGAGTCCG 1141 ATTCTTCTTC ATCAACGGGC CAGAAGCCAG // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl