RDB08308 - pET25b_Tr-egl1-His

Resource data sheet
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pET25b_Tr-egl1-His

Expression vector of Endoglucanase EG-I of Trichoderma reesei

Catalog number	RDB08308
Resource name	pET25b_Tr-egl1-His
Alternative name	pET25b_Tr003-His
Clone info.	Biomass collection. the Endoglucanase EG-I (EGLI, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone	pET-25b (Plasmid)
Size of vector backbone	5,547 bp
Selectable markers	Amp^r
Gene/insert name	Trichoderma reesei egl1 cDNA
Depositor\|Developer	DNA Bank, \| Kishikawa, Shotaro \|

Other clones in our bank

External Database
Trichoderma reesei egl1

Reference sequence

Sequence	RDB08308z.seq
Remarks, protocol and/or map (pdf)	RDB08308.pdf

Distribution information

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08308	pET25b_Tr-egl1-His	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET25b_Tr-egl1-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08308).

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	Biomass Collection (English text)
Featured content	Biomass Collection (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08308_A5Iip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB08308_A5Iia.seq
>08308_08308_A5Ii_T7_20bp_A01_01.ab1 1 TGCGGGTCAT TCCCCTCTAG AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATGA 61 AATACCTGCT GCCGACCGCT GCTGCTGGTC TGCTGCTCCT CGCTGCCCAG CCGGCGATGG 121 CCATGGATAT CGGAATTAAT TCGGATCCGA ATTCGAGCTC CGTCGACCAG CAACCGGGTA 181 CCAGCACCCC CGAGGTCCAT CCCAAGTTGA CAACCTACAA GTGTACAAAG TCCGGGGGGT 241 GCGTGGCCCA GGACACCTCG GTGGTCCTTG ACTGGAACTA CCGCTGGATG CACGACGCAA 301 ACTACAACTC GTGCACCGTC AACGGCGGCG TCAACACCAC GCTCTGCCCT GACGAGGCGA 361 CCTGTGGCAA GAACTGCTTC ATCGAGGGCG TCGACTACGC CGCCTCGGGC GTCACGACCT 421 CGGGCAGCAG CCTCACCATG AACCAGTACA TGCCCAGCAG CTCTGGCGGC TACAGCAGCG 481 TCTCTCCTCG GCTGTATCTC CTGGACTCTG ACGGTGAGTA CGTGATGCTG AAGCTCAACG 541 GCCAGGAGCT GAGCTTCGAC GTCGACCTCT CTGCTCTGCC GTGTGGAGAG AACGGCTCGC 601 TCTACCTGTC TCAGATGGAC GAGAACGGGG GCGCCAACCA GTATAACACG GCCGGTGCCA 661 ACTACGGGAG CGGCTACTGC GATGCTCAGT GCCCCGTCCA GACATGGAGG AACGGCACCC 721 TCAACACTAG CCACCAGGGC TTCTGCTGCA ACGAGATGGA TATCCTGGAG GGCAACTCGA 781 GGGCGAATGC CTTGACCCCT CACTCTTGCA CGGCCACGGC CTGCGACTCT GCCGGTTGCG 841 GCTTCAACCC CTATGGCAGC GGCTACAAAA GCTACTACGG CCCCGGAGAT ACCGTTGACA 901 CCTCCAAGAC CTTCACCATC ATCACCCAGT TCAACACGGA CAACGGCTCG CCCTCGGGCA 961 ACCTTGTGAG CATCACCCGC AAGTACCAGC AAAACGGCGT CGACATCCCC AGCGCCCAGC 1021 CCGGCGGCGA CACCATCTCG TCTGCTCGTC GTCTCAGCCT ACGGCGGCTC GCACATGGGC 1081 AAGTCCTGAG CCAGCGCATG TGCTCGTGAT CAGCCAATTG GGAAACGGAA CAACAAGCCA 1141 GGTATAACAA TGA //
Primer: f1ori_R3 Sequence file: RDB08308_A5Iib.seq
>08308_08308_A5Ii_f1ori_R3_B01_04.ab1 1 GATTGGCATC GGATTAGTTC CTCCTTTCAG CAAAACCCCT CAAGACCCGT TTAGAGGCCC 61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG 121 CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG CTCGACATCC TCGGGGTCTT 181 CCGGGGCGAG TTCTGGCTGG CTAGCCCGTT TGATCTCGAG TGCGGCCGCA AGCTTGTCGA 241 CAAGGCATTG CGAGTAGTAG TCGTTGCTAT ACTGGCACGT AGTGCCCGAC GTGCACGTCT 301 TGCACCCGCT GTACCCAATG CCACCGCACT GCCCCCAGTG AGTCTGCGTG CAGCTCGGGC 361 TGCTCGAAGT CGTCGAGCTC CTCCGTGTAG TCGAAAACGT CGTGCTGGAC GCAGGCGGGG 421 GCGGGGGCGC AGTCGAGTTC GTAGTAGACC CAATGTCTCC CCAGCGGATG TTGGAGAAGA 481 CGACGTGCGT GTTGGGGTTG TTGGCCAGGA TGTTGGATGG GTTGCCCTCG GTGCTGCTGC 541 AGGGGCCGGC GTTGCCGCTG TCGAGCCAGT TCATGTACTG GCTGTTGTCG TTCCAAATGC 601 TGAACACGAG CACCATGCCG CTGCTCAGGG CCTTGCCCAT GGTGGCGAGG CCGCCGTAGG 661 CTGAGGCGGA CGGGCAGGAC GAGATGGTGT CGCCGCCGGG CTGGGCGCTG GGGATGTCGA 721 CGCCGTTTTG CTGGTACTTG CGGGTGATGC TCACAAGGTT GCCCGAGGGC GAGCCGTTGT 781 CCGTGTTGAA CTGGGTGATG ATGGTGAAGG TCTTGGAGGT GTCAACGGTA TCTCCGGGGC 841 CGTAGTAGCT TTTGTAGCCG CTGCCATAGG GGTTGAAGCC GCAACCGGCA GAGTCGCAGG 901 CCGTGGCCGT GCAAGAGTGA GGGGTCAAGG CATTCGCCCT CGAGTTGCCC TCCAGGATAT 961 CCATCTCGTT GCAGCAGAAG CCCTGGTGGG CTAGTGTTTG AGGGTGCCGT TCCTCCATGT 1021 CTGGACGGGG CACTGGAGCA TCGCAGTAGC CGCCTCCCGT AGTGGCACCG GGCCGTGTTA 1081 TACTGGGTGG GCGCCCCCGA TTCTCGGTTC CAATCTGAGA ACAGTTAGAG GCGGAGTCCG 1141 ATTCTTCTTC ATCAACGGGC CAGAAGCCAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl