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pET25b_Tr-cbh2-His

Expression vector of Exoglucanase 2 of Trichoderma reesei

Catalog number RDB08307
Resource name pET25b_Tr-cbh2-His
Alternative name pET25b_Tr002-His
Clone info. Biomass collection. the Exoglucanase 2 (CBH2,1,4-beta-cellobiohydrolase Exocellobiohydrolase II (EC 3.2.1.91)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone pET-25b (Plasmid)
Size of vector backbone 5,547 bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei cbh2 cDNA
Depositor DNA Bank, | Kishikawa, Shotaro |

Distribution information

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RDB08307 pET25b_Tr-cbh2-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08307z.seq from Depositor
Electronic file PDF RDB08307.pdf from Depositor

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08307_A5Iip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08307_A5Iia.seq
>08307_08307_A5Ii_T7_20bp_G12_21.ab1
    1 TGCCGGACAT TCCCTCTAGA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACATATGAA
   61 ATACCTGCTG CCGACCGCTG CTGCTGGTCT GCTGCTCCTC GCTGCCCAGC CGGCGATGGC
  121 CATGGATATC GGAATTAATT CGGATCCGAA TTCGAGCTCC GTCGACGTGC CTCTAGAGGA
  181 GCGGCAAGCT TGCTCAAGCG TCTGGGGCCA ATGTGGTGGC CAGAATTGGT CGGGTCCGAC
  241 TTGCTGTGCT TCCGGAAGCA CATGCGTCTA CTCCAACGAC TATTACTCCC AGTGTCTTCC
  301 CGGCGCTGCA AGCTCAAGCT CGTCCACGCG CGCCGCGTCG ACGACTTCTC GAGTATCCCC
  361 CACAACATCC CGGTCGAGCT CCGCGACGCC TCCACCTGGT TCTACTACTA CCAGAGTACC
  421 TCCAGTCGGA TCGGGAACCG CTACGTATTC AGGCAACCCT TTTGTTGGGG TCACTCCTTG
  481 GGCCAATGCA TATTACGCCT CTGAAGTTAG CAGCCTCGCT ATTCCTAGCT TGACTGGAGC
  541 CATGGCCACT GCTGCAGCAG CTGTCGCAAA GGTTCCCTCT TTTATGTGGC TAGATACTCT
  601 TGACAAGACC CCTCTCATGG AGCAAACCTT GGCCGACATC CGCACCGCCA ACAAGAATGG
  661 CGGTAACTAT GCCGGACAGT TTGTGGTGTA TGACTTGCCG GATCGCGATT GCGCTGCCCT
  721 TGCCTCGAAT GGCGAATACT CTATTGCCGA TGGTGGCGTC GCCAAATATA AGAACTATAT
  781 CGACACCATT CGTCAAATTG TCGTGGAATA TTCCGATATC CGGACCCTCC TGGTTTATTG
  841 AGCCTGACTC TCTTGCCAAC CTGGTGACCA ACCTCGGTAC TCCAAAGTGT GCCAATGCTC
  901 AGTCAGCCTA CCTTGAGTGC ATCAACTACG CCCGTCACAC AGCTGAACCT TCCAAATGTT
  961 GCGATGTATT TGGACGCTGG CCATGCAGGA TGGCTTGGCT GGCCGGCAAA CCAGACCCCG
 1021 CCGCTCAGCT ATTTGCAATG TTACAGATGC ATCGTCTCGA GAGCTCTTCG CGATGCCACA
1081 TGTCGCACTA CACGGTGACA TACCAGCCTC CATCGTACAC GCCAGCCAAC GCTGATC
//
Primer: f1ori_R3 RDB08307_A5Iib.seq
>08307_08307_A5Ii_f1ori_R3_H12_24.ab1
    1 GAATCGCATC GGATTAGTTC CTCCTTTCAG CAAAAACCCC TCAAAGACCC GTTTAGAGGC
   61 CCCAAGGGGT TATGCTAGTT ATTGCTCAGC GGTGGCAGCA GCCAACTCAG CTTCCTTTCG
  121 GGCTTTGTTA GCAGCCGGAT CTCAGTGGTG GTGGTGGTGG TGCTCGACAT CCTCGGGGTC
  181 TTCCGGGGCG AGTTCTGGCT GGCTAGCCCG TTTGATCTCG AGTGCGGCCG CAAGCTTGTC
  241 GACCAGGAAC GATGGGTTTG CGTTTGTGAG AAGCTGCACA AAGTAGGCTT GGAACCAAGC
  301 ACCAGCTTGA GGCGCCGGTT GCAAGGCATC TGGGAGCGCA CAGTGGGAGT CAAATCGTGG
  361 CGCACTGCTG TCGCTGGTGC CGTCACACTC GCCGCCTGGC TTGACCCAGA CAAACGAATC
  421 CAGCAACGAG TCCCCAGTGT TTGCGGATGG GCGAATACCA AATCCGGTGC CGATCACATT
  481 GCACCAGTCT CCCCACTGTT GCTGTCCGGT AGGCTGCTTT CCCGATCGAC CTTGATCAGT
  541 GATGAAGAAG GCGTTGGACC AGCCGTGATT GGCAAGAAGA GGTCCAATAG CGTGGATGTA
  601 CAGCTTCTCG TTGTAGACAG CGTTGCCTTG CGTGTACGAT GGGGGGCTGG TAATGTTCCA
  661 CCCGTTGTAG TTGGCGACAT TGGTTGCCAA TCCGCGAAGA GCTCTCGGAG ACGATGCATT
  721 CTTGTAAACA TTTGCAAATA GCTGAGCGGC CGGGTCTTGG TTTGCCGGCC AGCCAAGCCA
  781 TCCTGCATGG CCAGCGTCCA AATACATCGC AACATTTGGA AGGTTCAGCT GTGTGACGGC
  841 GTAGTTGATG CACTCAAGGT AGGCTGACTG AGCATTGGCA CACTTTGGAG TACCGAGGTT
  901 GGTCACCAGG TTGGCAAGAG AGTCAGCTCA ATAACCAGGA GGGTCCGGAT ATCGGAATAT
  961 TCCCACGACA ATTTGACGAA TGGTGTCGAT ATAGTTCCTT ATATTTGGCG ACGCCACCAT
 1021 CGGCATAGAG TATTCGCCAA TTCGAAGCAG GGCAGCGCAT CGGCGATCCC GGCAAGTCAA
1081 TACATCCAAC CAAACTGTCA GGCATAAGTT ACGGCAATCT ATGTAGAC
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl