Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pET25b_Tr-cbh1-His (#RDB08306)
Expression vector of Exoglucanase 1 of Trichoderma reesei
| Alternative name | pET25b_Tr001-His |
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| Clone info. | Biomass collection. the Exoglucanase 1 (CBH1, 1,4-beta-cellobiohydrolase Exocellobiohydrolase I (EC 3.2.1.91)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b. |
| Vector backbone | pET-25b (Plasmid) |
| Size of vector backbone | 5,547 bp |
| Selectable markers | Amp^r |
| Gene/insert name | Trichoderma reesei cbh1 cDNA |
| Depositor|Developer | DNA Bank, | Kishikawa, Shotaro | |
| Sequence | RDB08306z.seq | Remarks, protocol and/or map (pdf) | RDB08306.pdf |
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Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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| Terms and conditions for distribution | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
|---|---|
| MTAに書く使用条件 | 理研BRCへの謝辞の表明を必要とする。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08306 | pET25b_Tr-cbh1-His | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pET25b_Tr-cbh1-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08306). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB08306_A5Iip1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: T7_20bp Sequence file: RDB08306_A5Iia.seq
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>08306_08306_A5Ii_T7_20bp_E12_15.ab1 1 GAAGGGATGG GTTGGGGGGT TCTAGAAATA GGGTTGTTTA CTTTAAGAAG GACGATATAC 61 ATATGAAATA CCTGCTGCCG ACCGCTGCTG CTGGTCTGCT GCTCCTCGCT GCCCAGCCGG 121 CGATGGCCAT GGATATCGGA ATTAATTCGG ATCCGAATTC GAGCTCCGTC GACCAGTCGG 181 CCTGCACTCT CCAATCGGAG ACTCACCCGC CTCTGACATG GCAGAAATGC TCGTCTGGTG 241 GCACGTGCAC TCAACAGACA GGCTCCGTGG TCATCGACGC CAACTGGCGC TGGACTCACG 301 CTACGAACAG CAGCACGAAC TGCTACGATG GCAACACTTG GAGCTCGACC CTATGTCCTG 361 ACAACGAGAC CTGCGCGAAG AACTGCTGTC TGGACGGTGC CGCCTACGCG TCCACGTACG 421 GAGTTACCAC GAGCGGTAAC AGCCTCTCCA TTGGCTTTGT CACCCAGTCT GCGCAGAAGA 481 ACGTTGGCGC TCGCCCTTTA CCTTATGGCG AGCGACACGA CCTACCCAGG AATTCACCCT 541 GCTTGGCAAC GAAGTTTCTC TTTCGATGTT GATGTTTCGC AGCTGCCGTG CGGCTTGAAC 601 GGAGCTCTCT ACTTCGTGTC CAATGGACGC GGATGGTGGC GTGAGCAAGT ATCCCACCAA 661 CACCGCTGGC GCCAAGTACG GCACGGGGGT ACTGTGACAG CCAGTGTCCC CGCGATCTGA 721 AGTTCATCAA TGGCCAGGCC AACGTTGAGG GCTGGGAGCC GTCATCCAAC AACGCGAACA 781 CGGGCATTGA GGACACGGAA GCTGCTGCTC TGGAGATGGA TATCTGGGAG GCCAACTCCA 841 TCTCCGAGGC TCT // |
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Primer: f1ori_R3 Sequence file: RDB08306_A5Iib.seq
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>08306_08306_A5Ii_f1ori_R3_F12_18.ab1 1 GGATTCCATC GGGATTAGTT CTCCTTTCAG CAAAAACCCC TCAAGACCCG TTTAGAGGCC 61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG 121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGACATC CTCGGGGTCT 181 TCCGGGGCGA GTTCTGGCTG GCTAGCCCGT TTGATCTCGA GTGCGGCCGC AAGCTTGTCG 241 ACCAGGCACT GAGAGTAGTA AGGGTTCAGG ACCTGGCAAG TTGTGCCGCT GGCGCAGACC 301 GTGGGGCCGC TGTAGCCAAT ACCGCCGCAC TGGCCGTAGT GAGACTGGGT AGGTCCGGGA 361 GAGCTTCCAG TGGTAGTGGC TGGGCGGCGG GTGGTGGTGG TGCCAGGCGG GTTTCCGCCG 421 GGAGGGTTGC CGCCGCTAGG GTTGCCGGTG CTGCCAATGG GTCCGAACTT GATGTTGGAG 481 AAGGTGACCT TGGCGTTGGG AGACTGAGAT TCGACCTGAG CAGGGACACC GGAGCTGGTG 541 GAGCAGCTTC CGCGCACGGC ACCGGGTGTG GAGGAGGTCT CGTTTGTCGG GTAGGTGGAG 601 TCCAGCCACA GCATGTTGGC GTAGTAATCA TCCCACAGAC TCATGACCAG AACCATGCCG 661 CCAGAGGTAG CCTTCTTGAA CTGAGTCAGG CCGCCCTTGT CTGAGAAAGA GGATCCGCCG 721 AATTCTGCCT CCTCAGCTGT GCAGTAATCA TCGTTGAGCT CGTTGCCAGA GTAACTACCC 781 AGCTCGGCGT TGGGCTGCTG GAAAGTGACG CCATTCTGGA CATAGTATCG GTTGATGGGC 841 ACCCGACGTC TCGAACTGGG TGACACGGGT CAATTTCTTG TGTATCGAGG GTAAGCTGGA 901 GCCAGGCGGT AGAGCTGTGT TGCTCAAGCG GTATGGTTCA GTCGCTAGCA TCGGATCGCA 961 AGTGCCGCAT ACTGTAATCG GAGCTAAAGT TCCGGCGGCA CCATCACCTC GA // |
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Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Trichoderma reesei cbh1
Reference sequence
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- Biomass Collection (English text)
- Biomass Collection (Japanese text)