Resource data sheet
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pET25b_Tr-cbh1-His

Expression vector of Exoglucanase 1 of Trichoderma reesei

Catalog number RDB08306
Resource name pET25b_Tr-cbh1-His
Alternative name pET25b_Tr001-His
Clone info. Biomass collection. the Exoglucanase 1 (CBH1, 1,4-beta-cellobiohydrolase Exocellobiohydrolase I (EC 3.2.1.91)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone pET-25b (Plasmid)
Size of vector backbone 5,547 bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei cbh1 cDNA
Depositor DNA Bank, | Kishikawa, Shotaro |

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08306 pET25b_Tr-cbh1-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08306z.seq from Depositor
Electronic file PDF RDB08306.pdf from Depositor

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08306_A5Iip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08306_A5Iia.seq
>08306_08306_A5Ii_T7_20bp_E12_15.ab1
    1 GAAGGGATGG GTTGGGGGGT TCTAGAAATA GGGTTGTTTA CTTTAAGAAG GACGATATAC
   61 ATATGAAATA CCTGCTGCCG ACCGCTGCTG CTGGTCTGCT GCTCCTCGCT GCCCAGCCGG
  121 CGATGGCCAT GGATATCGGA ATTAATTCGG ATCCGAATTC GAGCTCCGTC GACCAGTCGG
  181 CCTGCACTCT CCAATCGGAG ACTCACCCGC CTCTGACATG GCAGAAATGC TCGTCTGGTG
  241 GCACGTGCAC TCAACAGACA GGCTCCGTGG TCATCGACGC CAACTGGCGC TGGACTCACG
  301 CTACGAACAG CAGCACGAAC TGCTACGATG GCAACACTTG GAGCTCGACC CTATGTCCTG
  361 ACAACGAGAC CTGCGCGAAG AACTGCTGTC TGGACGGTGC CGCCTACGCG TCCACGTACG
  421 GAGTTACCAC GAGCGGTAAC AGCCTCTCCA TTGGCTTTGT CACCCAGTCT GCGCAGAAGA
  481 ACGTTGGCGC TCGCCCTTTA CCTTATGGCG AGCGACACGA CCTACCCAGG AATTCACCCT
  541 GCTTGGCAAC GAAGTTTCTC TTTCGATGTT GATGTTTCGC AGCTGCCGTG CGGCTTGAAC
  601 GGAGCTCTCT ACTTCGTGTC CAATGGACGC GGATGGTGGC GTGAGCAAGT ATCCCACCAA
  661 CACCGCTGGC GCCAAGTACG GCACGGGGGT ACTGTGACAG CCAGTGTCCC CGCGATCTGA
  721 AGTTCATCAA TGGCCAGGCC AACGTTGAGG GCTGGGAGCC GTCATCCAAC AACGCGAACA
  781 CGGGCATTGA GGACACGGAA GCTGCTGCTC TGGAGATGGA TATCTGGGAG GCCAACTCCA
841 TCTCCGAGGC TCT
//
Primer: f1ori_R3 RDB08306_A5Iib.seq
>08306_08306_A5Ii_f1ori_R3_F12_18.ab1
    1 GGATTCCATC GGGATTAGTT CTCCTTTCAG CAAAAACCCC TCAAGACCCG TTTAGAGGCC
   61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG
  121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGACATC CTCGGGGTCT
  181 TCCGGGGCGA GTTCTGGCTG GCTAGCCCGT TTGATCTCGA GTGCGGCCGC AAGCTTGTCG
  241 ACCAGGCACT GAGAGTAGTA AGGGTTCAGG ACCTGGCAAG TTGTGCCGCT GGCGCAGACC
  301 GTGGGGCCGC TGTAGCCAAT ACCGCCGCAC TGGCCGTAGT GAGACTGGGT AGGTCCGGGA
  361 GAGCTTCCAG TGGTAGTGGC TGGGCGGCGG GTGGTGGTGG TGCCAGGCGG GTTTCCGCCG
  421 GGAGGGTTGC CGCCGCTAGG GTTGCCGGTG CTGCCAATGG GTCCGAACTT GATGTTGGAG
  481 AAGGTGACCT TGGCGTTGGG AGACTGAGAT TCGACCTGAG CAGGGACACC GGAGCTGGTG
  541 GAGCAGCTTC CGCGCACGGC ACCGGGTGTG GAGGAGGTCT CGTTTGTCGG GTAGGTGGAG
  601 TCCAGCCACA GCATGTTGGC GTAGTAATCA TCCCACAGAC TCATGACCAG AACCATGCCG
  661 CCAGAGGTAG CCTTCTTGAA CTGAGTCAGG CCGCCCTTGT CTGAGAAAGA GGATCCGCCG
  721 AATTCTGCCT CCTCAGCTGT GCAGTAATCA TCGTTGAGCT CGTTGCCAGA GTAACTACCC
  781 AGCTCGGCGT TGGGCTGCTG GAAAGTGACG CCATTCTGGA CATAGTATCG GTTGATGGGC
  841 ACCCGACGTC TCGAACTGGG TGACACGGGT CAATTTCTTG TGTATCGAGG GTAAGCTGGA
  901 GCCAGGCGGT AGAGCTGTGT TGCTCAAGCG GTATGGTTCA GTCGCTAGCA TCGGATCGCA
961 AGTGCCGCAT ACTGTAATCG GAGCTAAAGT TCCGGCGGCA CCATCACCTC GA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl