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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET21c_Tr-egl2-His

Expression vector of Endoglucanase EG-II of Trichoderma reesei

Catalog number RDB08289
Resource name pET21c_Tr-egl2-His
Alternative name pET21c_Tr004-His
Clone info. Biomass collection. the Endoglucanase EG-II (EGLII, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c.
Vector backbone pET-21c (Plasmid)
Size of vector backbone 5441bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei egl2 cDNA
Depositor|Developer DNA Bank, | Kishikawa, Shotaro |

Distribution information

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RDB08289 pET21c_Tr-egl2-His DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB08289z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB08289.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17894_B0A6p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F
Region: T7 pro, lacO, RBS, T7 tag, insert5'
Sequence file: RDB17894_B0A6a.seq check
>08289_17894_B0A6_1_pET-B_F_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNNNG CCGGCNNCGA TGCGTCCGGC GTAGAGGNNC GAGNNCTCGA
   61 TCCCGCGAAA TTAATACGAC TCACTATAGG GGAATTGTGA GCGGATAACA ATTCCCCTCT
  121 AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGGCTAGCAT GACTGGTGGA
  181 CAGCAAATGG GTCGGATCCG AATTCGAGCT CCGTCGACCC AGCAGACTGT CTGGGGCCAG
  241 TGTGGAGGTA TTGGTTGGAG CGGACCTACG AATTGTGCTC CTGGCTCAGC TTGTTCGACC
  301 CTCAATCCTT ATTATGCGCA ATGTATTCCG GGAGCCACTA CTATCACCAC TTCGACCCGG
  361 CCACCATCCG GTCCAACCAC CACCACCAGG GCTACCTCAA CAAGCTCATC AACTCCACCC
  421 ACGAGCTCTG GGGTCCGATT TGCCGGCGTT AACATCGCGG GTTTTGACTT TGGCTGTACC
  481 ACAGATGGCA CTTGCGTTAC CTCGAAGGTT TATCCTCCGT TGAAGAACTT CACCGGCTCA
  541 AACAACTACC CCGATGGCAT CGGCCAGATG CAGCACTTCG TCAACGACGA CGGGATGACT
  601 ATTTTCCGCT TACCTGTCGG ATGGCAGTAC CTCGTCAACA ACAATTTGGG CGGCAATCTT
  661 GATTCCACGA GCATTTCCAA GTATGATCAG CTTGTTCAGG GGTGCCTGTC TCTGGGCGCA
  721 TACTGCATCG TCGACATCCA CAATTATGCT CGATGGAACG GTGGGATCAT TGGTCAGGGC
  781 GGCCCTACTA ATGCTCAATT CACGANCCTT TGGTCGCAGT TGGCATCAAA GTACGCATCT
  841 CAGTCGAGGG NGTGGTTCGG CATCATGAAT GAGCCCCACN ANNNGAACAT CAACANCNNN
  901 NNNGCCACNG NCCNNNANGT TGTAANCGCN NNCCGCNNCG CNGNNNCTAN NNCNNANNNN
961 NNNNNN
//
Primer: f1ori_R3
Region: T7 ter, 6xHis, insert3'
Sequence file: RDB17894_B0A6b.seq check
>08289_17894_B0A6_1_f1ori_R3_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNCCTTTNN GCNANNAACC CCTCAAGANC CGTTTAGAGG
   61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC
  121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGAGT GCGGCCGCAA
  181 GCTTGTCGAC CTTTCTTGCG AGACACGAGC TGACCAAGGA TGTGTCCGTC CATGAGTTAC
  241 CACTGCCAGT CGGTGTTTCC GTCAGGACAT ACGTGCTATC AAATGATCCG GCACCCCAAC
  301 CAACATAGCC AAGATAGACA TCTGAGTTCT GGTTGAGATA TTGGATTTGC TGGCACATGT
  361 CTTGTATGCA GGACTGAACG TTGCCACCAC CGGTTTCTGT CAGGATAGCC TGGCGATTGT
  421 TCTGTCGGAG CCAAGTGGCA AGCGGAGAAA AGGCGCCGTC AATGTTATTT GTAGTACATT
  481 CGGCGTGAGT ACCGGAGTTG TCTGAGTCCA AGTATTTGTG CACGTCAAAA ATCAGATTCG
  541 TTGTTGACCC ATCCGGGTTC GTGACTTGAG ACAGGGCGGC TGCACTGCCA TCGGATATGA
  601 AAGCCCCAGC AGATTGCCAA TCATTTCCAG GCAAAGAGAT GAATTGCGAC GTAGCACCAG
  661 CGTTGCGGAT TGCGGTTACA ACCTCTTGGA CCGTGGCAGC CCAGGTGTTG ATGTTCACGT
  721 CGTGGGGCTC ATTCATGATG CCGAACCACA CCCTCGACTG ANNTGCGTAC TTTGATGCCA
  781 ACTGCGACCA AAGGCTCGTG AATTGAGCAT TAGTANGGCC GCCCTGANCA ATGNTCCCAC
  841 CNNNCCATCG AGCATAATTG TGGATGTCGA CGATGCAGTA TGCGCCCAGA NANANNNACC
901 CNNNNNNAAN CNNNTCATAN NTNNAAATGN NCNNNNNNTN NNNNNNNNN
//
Primer: lacIpro_F2
Region: lacI gene
Sequence file: RDB17894_B0A6c.seq check
>08289_17894_B0A6_1_laclpro_F2_D08_11_ABI24.ab1
    1 NNNNNNNNNN NNNGNNGGNN NCGCATAAGG GANANCGTCG ANNTCCCGGN NACCATCGAA
   61 TGGCGCAAAA CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG
  121 GTGAATGTGA AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG
  181 ACCGTTTCCC GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG
  241 GAAGCGGCGA TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC
  301 AAACAGTCGT TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA
  361 ATTGTCGCGG CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG
  421 GTAGAACGAA GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC
  481 GTCAGTGGGC TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT
  541 GCCTGCACTA ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT
  601 ATTATTTTCT CCCATGAAGA CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT
  661 CACCAGCAAA TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG
  721 GCTGGCTGGC ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAANGC
  781 GACTGGAGTG CCATGTCCGG TTTTCAACAA ACCATGCNNN TGCTGAATGA NGGCATCGTT
  841 CCCACNNCGA TGCTGGTTGN NNACGATCAG ATGGCGCTGG GCGCAATGCG CGCNNTTACC
  901 NANNCCGGGC NGCNCGTTNG NGCNNNTATC TCNGNANNGG NANACNANNA NACCNNNNNN
961 NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.10.02

GNP_filter3_RDBDEP_html_200316.pl