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pET21c_Tr-egl2-His

Expression vector of Endoglucanase EG-II of Trichoderma reesei

Catalog number RDB08289
Resource name pET21c_Tr-egl2-His
Alternative name pET21c_Tr004-His
Clone info. Biomass collection. the Endoglucanase EG-II (EGLII, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c.
Vector backbone pET-21c (Plasmid)
Size of vector backbone 5441bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei egl2 cDNA
Depositor DNA Bank, | Kishikawa, Shotaro |

Distribution information

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RDB08289 pET21c_Tr-egl2-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08289z.seq from Depositor
Electronic file PDF RDB08289.pdf from Depositor

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08289_A6G4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08289_A6G4a.seq
>08289_08289_A6G4_T7_20bp_B07_04.ab1
    1 TCGAACATTC GCTCTAGAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCTA
   61 GCATGACTGG TGGACAGCAA ATGGGTCGGA TCCGAATTCG AGCTCCGTCG ACCCAGCAGA
  121 CTGTCTGGGG CCAGTGTGGA GGTATTGGTT GGAGCGGACC TACGAATTGT GCTCCTGGCT
  181 CAGCTTGTTC GACCCTCAAT CCTTATTATG CGCAATGTAT TCCGGGAGCC ACTACTATCA
  241 CCACTTCGAC CCGGCCACCA TCCGGTCCAA CCACCACCAC CAGGGCTACC TCAACAAGCT
  301 CATCAACTCC ACCCACGAGC TCTGGGGTCC GATTTGCCGG CGTTAACATC GCGGGTTTTG
  361 ACTTTGGCTG TACCACAGAT GGCACTTGCG TTACCTCGAA GGTTTATCCT CCGTTGAAGA
  421 ACTTCACCGG CTCAAACAAC TACCCCGATG GCATCGGCCA GATGCAGCAC TTCGTCAACG
  481 ACGACGGGAT GACTATTTTC CGCTTACCTG TCGGATGGCA GTACCTCGTC AACAACAATT
  541 TGGGCGGCAA TCTTGATTCC ACGAGCATTT CCAAGTATGA TCAGCTTGTT CAGGGGTGCC
  601 TGTCTCTGGG CGCATACTGC ATCGTCGACA TCCACAATTA TGCTCGATGG AACGGTGGGA
  661 TCATTGGTCA GGGCGGCCCT ACTAATGCTC AATTCACGAG CCTTTGGTCG CAGTTGGCAT
  721 CAAAGTACGC ATCTCAGTCG AGGGTGTGGT TCGGCATCAT GAATGAGCCC CACGACGTGA
  781 ACATCAACAC CTGGGCTGCC ACGGTCCAAG AGGTTGTAAC CGCAATCCGC AACGCTGGTG
  841 CTACGTCGCA ATTCATCTCT TTGCCTGGAA ATGATTGGCA ATCTGCTGGG GCTTTCATAT
  901 CCGATGGCAG TGCAGCCGCC CTGTCTCAAG TCACGAACCC GGATGGGTCA ACAACGAATC
  961 TGATTTTTTG ACGTGCACAA ATACTTGGAC TCAGACACTC CCGGGTACTC ACGCCCGAAT
 1021 GTACTACAAT AACATTTGAC GGCGCCTTTT TCTCCGCCTT GCAACTGGCT CCGACAGAAC
1081 AATCGACAGC TATCTTGACA GAACCCGGTG GTGGACAACG GATTCAGCTC
//
Primer: f1ori_R3 RDB08289_A6G4b.seq
>08289_08289_A6G4_f1ori_R3_F07_16.ab1
    1 GGGGGGGCAT CGGGGATTAG TTCCTCCTTT CAGCAAAAAC CCCTCAAGAC CCGTTTAGAG
   61 GCCCCAAGGG GTTATGCTAG TTATTGCTCA GCGGTGGCAG CAGCCAACTC AGCTTCCTTT
  121 CGGGCTTTGT TAGCAGCCGG ATCTCAGTGG TGGTGGTGGT GGTGCTCGAG TGCGGCCGCA
  181 AGCTTGTCGA CCTTTCTTGC GAGACACGAG CTGACCAAGG ATGTGTCCGT CCATGAGTTA
  241 CCACTGCCAG TCGGTGTTTC CGTCAGGACA TACGTGCTAT CAAATGATCC GGCACCCCAA
  301 CCAACATAGC CAAGATAGAC ATCTGAGTTC TGGTTGAGAT ATTGGATTTG CTGGCACATG
  361 TCTTGTATGC AGGACTGAAC GTTGCCACCA CCGGTTTCTG TCAGGATAGC CTGGCGATTG
  421 TTCTGTCGGA GCCAAGTGGC AAGCGGAGAA AAGGCGCCGT CAATGTTATT TGTAGTACAT
  481 TCGGCGTGAG TACCGGAGTT GTCTGAGTCC AAGTATTTGT GCACGTCAAA AATCAGATTC
  541 GTTGTTGACC CATCCGGGTT CGTGACTTGA GACAGGGCGG CTGCACTGCC ATCGGATATG
  601 AAAGCCCCAG CAGATTGCCA ATCATTTCCA GGCAAAGAGA TGAATTGCGA CGTAGCACCA
  661 GCGTTGCGGA TTGCGGTTAC AACCTCTTGG ACCGTGGCAG CCCAGGTGTT GATGTTCACG
  721 TCGTGGGGCT CATTCATGAT GCCGAACCAC ACCCTCGACT GAGATGCGTA CTTTGATGCC
  781 AACTGCGACC AAAGGCTCGT GAATTGAGCA TTAGTAGGGC CGCCCTGACC AATGATCCCA
  841 CCGTTCCATC GAGCATAATT GTGGATGTCG ACGATGCAGT ATGCGCCCAG AGACAGGCAC
  901 CCCTGAACAA GCTGATCATA CTTGGAAATG CTCGTGGAAT CAAGATTGCC GCCCAAATTG
  961 TTGTTGACGA GGTACTGCCA TCCGACAGGG TAAGCGGAAA AATAGTCATC CCGTCGTCGT
 1021 TTGACGAAGT GCTGCAATCC TGGGCCGATG CCCATCGGGG GTAGTTTGTT TGAGCCCGGT
 1081 GAGGTCTTTC AACGGAGGAA TATCCATCGA GTTAACGGCA AGTGGCCATT CTTGTTGGGT
1141 AACAAGTCCC A
//
Primer: pGL3_RV4 RDB08289_A6G4c.seq
>08289_08289_A6G4_pGL3_RV4_B08_05.ab1
    1 GTATACTCTG GGTTGAGGCT CTCAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG
   61 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
  121 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA
  181 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC
  241 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT
  301 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA
  361 CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG
  421 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA
  481 TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA
  541 TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC
  601 TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC
  661 CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA
  721 CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT
  781 CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG
  841 CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA
  901 GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGGCGCGTGC AGGGCCAGAC
  961 TGGAGGTGGC AACGCCCAAT CAGCAACGAC TGTTTGCCCG CCAGTTTGTT GTGCCACGCG
 1021 GGTTGGGAAA TGTAAATTCA GCTCCGCCCA TCGCCCGCTT CCACTTTTTC CCGCGTTTTT
 1081 CGCAGAAACC GTTGCCTGCT GATTCACCAC GCGGGAAACG GTTCTTGGAA TAAGGAAGAA
1141 CA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl