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pET21c_Tr-cbh2-His

Expression vector of Exoglucanase 2 of Trichoderma reesei

Catalog number RDB08287
Resource name pET21c_Tr-cbh2-His
Alternative name pET21c_Tr002-His
Clone info. Biomass collection. the Exoglucanase 2 (CBH2,1,4-beta-cellobiohydrolase Exocellobiohydrolase II (EC 3.2.1.91)) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c.
Vector backbone pET-21c (Plasmid)
Size of vector backbone 5441bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei cbh2 cDNA
Depositor DNA Bank, | Kishikawa, Shotaro |

Distribution information

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RDB08287 pET21c_Tr-cbh2-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08287z.seq from Depositor
Electronic file PDF RDB08287.pdf from Depositor

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08287_A6Afp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08287_A6Afa.seq
>08287_08287_A6Af_T7_20bp_E03_15.ab1
    1 TCCCCAGGTT CAATTCCCCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC
   61 ATATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGAT CCGAATTCGA GCTCCGTCGA
  121 CCGTGCCTCT AGAGGAGCGG CAAGCTTGCT CAAGCGTCTG GGGCCAATGT GGTGGCCAGA
  181 ATTGGTCGGG TCCGACTTGC TGTGCTTCCG GAAGCACATG CGTCTACTCC AACGACTATT
  241 ACTCCCAGTG TCTTCCCGGC GCTGCAAGCT CAAGCTCGTC CACGCGCGCC GCGTCGACGA
  301 CTTCTCGAGT ATCCCCCACA ACATCCCGGT CGAGCTCCGC GACGCCTCCA CCTGGTTCTA
  361 CTACTACCAG AGTACCTCCA GTCGGATCGG GAACCGCTAC GTATTCAGGC AACCCTTTTG
  421 TTGGGGTCAC TCCTTGGGCC AATGCATATT ACGCCTCTGA AGTTAGCAGC CTCGCTATTC
  481 CTAGCTTGAC TGGAGCCATG GCCACTGCTG CAGCAGCTGT CGCAAAGGTT CCCTCTTTTA
  541 TGTGGCTAGA TACTCTTGAC AAGACCCCTC TCATGGAGCA AACCTTGGCC GACATCCGCA
  601 CCGCCAACAA GAATGGCGGT AACTATGCCG GACAGTTTGT GGTGTATGAC TTGCCGGATC
  661 GCGATTGCGC TGCCCTTGCC TCGAATGGCG AATACTCTAT TGCCGATGGT GGCGTCGCCA
  721 AATATAAGAA CTATATCGAC ACCATTCGTC AAATTGTCGT GGAATATTCC GATATCCGGA
  781 CCCTCCTGGT TATTGAGCCT GACTCTCTTG CCAACCTGGT GACCAACCTC GGTACTCCAA
  841 AGTGTGCCAA TGCTCAGTCA GCCTACCTTG AGTGCATCAA CTACGCCGTC ACACAGCTGA
  901 ACCTTCCAAA TGTTGCGATG TATTTGGACG CTGGCCATGC AGGATGGCTT GGCTGGCCGG
  961 CAAACCAAGA CCCGGCCCGC TCAGCTATTT GCAATGTTTA CAGAATGCAT CGTCTCCGAG
 1021 AGCTCTTCGC GGAATTGGCA ACCCAATGTC GCCCAACTAC ACGGGGTGAC CATTTACCCA
 1081 GCCCCCTCAT CGTACACGCA AGGCAACGGC TGTCTACCAC CGAGAAGCCT GGTACATTCA
1141 CGCTAATTGG ACCCTTCTTT CTTTGTGCCA ATC
//
Primer: f1ori_R3 RDB08287_A6Afb.seq
>08287_08287_A6Af_f1ori_R3_F03_18.ab1
    1 TGGGTCGCCA ATCGGAAAAG TTTCTCCTTT CAGCAAAAAA CCCCCTCAAG GACCCGTTTA
   61 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAAGCGGTG GCAGCAGCCA ACTCAGCTTC
  121 CTTTCGGGCT TTGTTAGCAG CCGGATCTCA GTGGTGGTGG TGGTGGTGCT CGAGTGCGGC
  181 CGCAAGCTTG TCGACCAGGA ACGATGGGTT TGCGTTTGTG AGAAGCTGCA CAAAGTAGGC
  241 TTGGAACCAA GCACCAGCTT GAGGCGCCGG TTGCAAGGCA TCTGGGAGCG CACAGTGGGA
  301 GTCAAATCGT GGCGCACTGC TGTCGCTGGT GCCGTCACAC TCGCCGCCTG GCTTGACCCA
  361 GACAAACGAA TCCAGCAACG AGTCCCCAGT GTTTGCGGAT GGGCGAATAC CAAATCCGGT
  421 GCCGATCACA TTGCACCAGT CTCCCCACTG TTGCTGTCCG GTAGGCTGCT TTCCCGATCG
  481 ACCTTGATCA GTGATGAAGA AGGCGTTGGA CCAGCCGTGA TTGGCAAGAA GAGGTCCAAT
  541 AGCGTGGATG TACAGCTTCT CGTTGTAGAC AGCGTTGCCT TGCGTGTACG ATGGGGGGCT
  601 GGTAATGTTC CACCCGTTGT AGTTGGCGAC ATTGGTTGCC AATCCGCGAA GAGCTCTCGG
  661 AGACGATGCA TTCTTGTAAA CATTTGCAAA TAGCTGAGCG GCCGGGTCTT GGTTTGCCGG
  721 CCAGCCAAGC CATCCTGCAT GGCCAGCGTC CAAATACATC GCAAACATTT GGAAGGTTCA
  781 GCTGTGTGAC GGCGTAGTTG ATGCACTCAA GGTAGGCTGA CTGAGCATTG GCACACTTTG
  841 GAGTACCGAG GTTGGTCACC AGGTTGGGCA AGAGAGTCAG GCTCAATAAC CAGGGAGGGT
  901 CCCGGATATC GGGAATAATT CCACGACAAT TTGACGAATG GTGTCGATAT AGTTCTTATA
  961 TTTGGGCGAC GCCACCATCG GCAATAGAGT AATTCGCCCA TTCGAGGCAA AGGGCAGCGC
 1021 CAATCGCGAA TCCGGCAAGG TCATACCACA ACAACTGGTC CGGGCATTAG TTACCGCCAA
 1081 TTCTGTTGGC GGGTGCCGGA TGTCGGGCAG TTTTGCTCAA TGAGAGGGGT CTTGTCAGAG
1141 TATCTAAGCT ACATAAAAGG AAAGGGGAAA CCTTTTGGGC GACACGCCGG CC
//
Primer: pGL3_RV4 RDB08287_A6Afc.seq
>08287_08287_A6Af_pGL3_Rv4_G03_21.ab1
    1 GGAGGGGCAT GAAATGAGGT TGAGGCTCTC AGGGCATCGG TCGAGATCCC GGTGCCTAAT
   61 GAGTGAGCTA ACTTACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
  121 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
  181 GGCGCCAGGG TGGTTTTTCT TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC
  241 GCCTGGCCCT GAGAGAGTTG CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA
  301 TCCTGTTTGA TGGTGGTTAA CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT
  361 CCCACTACCG AGATATCCGC ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG
  421 CCCAGCGCCA TCTGATCGTT GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC
  481 ATTTGCATGG TTTGTTGAAA ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC
  541 GGCTGAATTT GATTGCGAGT GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG
  601 ACAGAACTTA ATGGGCCCGC TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC
  661 TCCACGCCCA GTCGCGTACC GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG
  721 TCAGAGACAT CAAGAAATAA CGCCGGAACA TTAGTGCAGG CAGCTTCCAC AGCAATGGCA
  781 TCCTGGTCAT CCAGCGGATA GTTAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG
  841 TGCACCGCCG CTTTACAGGC TTCGACGCCG CTTCGTTCTA CCATCGACAC CACCACGCTG
  901 GCACCCAGTT GATCGGCGCG AGATTTAATC GCCGCGACAA TTTGCGACGG CGCGTGCAGG
  961 GCCAGACTGG AGGTGGCAAC GCCCAATCAG CAACGACTGT TTGCCCGCCC AGTTTGTTGT
 1021 GCCACGCGGA TGGGGAATGT AATTCAGCTC CGCCCATCGC CGCTTCCACT TTTTCCCCGC
 1081 GTTTTCCGCA GAAACGTGAC TGGCTGGATT TCACCACGCG GAACCGGTCT GATAAGAGAC
1141 ACCGGCCATA CTCTTGCCGA ACAATCGTAA TTTAAG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl