Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET21c_Tr-cbh1-His

Expression vector of Exoglucanase 1 of Trichoderma reesei

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08286_A5Lap1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB08286_A5Laa.seq
>08286_08286_A5La_T7_20bp_A12_03.ab1     1 TTCAAGAACC ATTTCCCCTT CTAGAATAAT TTTTGTTTAA CTTTAAGAAG GGAGATATAC    61 ATAATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGA TCCGAATTCG AGCTCCGTCG   121 ACCCAGTCGG CCTGCACTCT CCAATCGGAG ACTCACCCGC CTCTGACATG GCAGAAATGC   181 TCGTCTGGTG GCACGTGCAC TCAACAGACA GGCTCCGTGG TCATCGACGC CAACTGGCGC   241 TGGACTCACG CTACGAACAG CAGCACGAAC TGCTACGATG GCAACACTTG GAGCTCGACC   301 CTATGTCCTG ACAACGAGAC CTGCGCGAAG AACTGCTGTC TGGACGGTGC CGCCTACGCG   361 TCCACGTACG GAGTTACCAC GAGCGGTAAC AGCCTCTCCA TTGGCTTTGT CACCCAGTCT   421 GCGCAGAAGA ACGTTGGCGC TCGCCTTTAC CTTATGGCGA GCGACACGAC CTACCAGGAA   481 TTCACCCTGC TTGGCAACGA GTTCTCTTTC GATGTTGATG TTTCGCAGCT GCCGTGCGGC   541 TTGAACGGAG CTCTCTACTT CGTGTCCATG GACGCGGATG GTGGCGTGAG CAAGTATCCC   601 ACCAACACCG CTGGCGCCAA GTACGGCACG GGGTACTGTG ACAGCCAGTG TCCCCGCGAT   661 CTGAAGTTCA TCAATGGCCA GGCCAACGTT GAGGGCTGGG AGCCGTCATC CAACAACGCG   721 AACACGGGCA TTGGAGGACA CGGAAGCTGC TGCTCTGAGA TGGATATCTG GGAGGCCAAC   781 TCCATCTCCG AGGCTCTTAC CCCCCACCCT TGCACGGACT GTCGGCCAGG AGATCTGCGA   841 GGGTGATGGG GTGCGGCGGA ACTTACTCCG ATAACAGATA TGGGCGGCAC TTGCGATCCC   901 GATGGCTGCG AACTGGAACC CATACCGCCT GGGCAAACAC CAGCTTCTAC GGCCCCTGGC   961 TCAAAGCCTT TACCCTCGAA TACCACCAAG AAAATTGACC GGTTGGTCAA CCCAAGTTTC  1021 GAAGAACGTC CGGGTGCCCA TCAACCGGAT TACCTAATGT CCCAGAAATG GCGGTCACTT  1081 TTCCAGCCAG CCCCAACGCC GGAAGCTTGG TAAGTTTACT TCTTGGGCAA CGAGACCTCT 1141 CCAACACGGA ATGAGAATGT A //
Primer: f1ori_R3 Sequence file: RDB08286_A5Lab.seq
>08286_08286_A5La_f1ori_R3_B12_06.ab1     1 GCATGCATCG GGATTAGTTC CTCCTTTCAG CAAAAACCCC TCAAGACCCG TTTAGAGGCC    61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG   121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGAGTGC GGCCGCAAGC   181 TTGTCGACCA GGCACTGAGA GTAGTAAGGG TTCAGGACCT GGCAAGTTGT GCCGCTGGCG   241 CAGACCGTGG GGCCGCTGTA GCCAATACCG CCGCACTGGC CGTAGTGAGA CTGGGTAGGT   301 CCGGGAGAGC TTCCAGTGGT AGTGGCTGGG CGGCGGGTGG TGGTGGTGCC AGGCGGGTTT   361 CCGCCGGGAG GGTTGCCGCC GCTAGGGTTG CCGGTGCTGC CAATGGGTCC GAACTTGATG   421 TTGGAGAAGG TGACCTTGGC GTTGGGAGAC TGAGATTCGA CCTGAGCAGG GACACCGGAG   481 CTGGTGGAGC AGCTTCCGCG CACGGCACCG GGTGTGGAGG AGGTCTCGTT TGTCGGGTAG   541 GTGGAGTCCA GCCACAGCAT GTTGGCGTAG TAATCATCCC ACAGACTCAT GACCAGAACC   601 ATGCCGCCAG AGGTAGCCTT CTTGAACTGA GTCAGGCCGC CCTTGTCTGA GAAAGAGGAT   661 CCGCCGAATT CTGCCTCCTC AGCTGTGCAG TAATCATCGT TGAGCTCGTT GCCAGAGTAA   721 CTACCAAGCT CGGCGTTGGG CTGCTGGAAA GTGACGCCAT TCTGGACATA GTATCGGTTG   781 ATGGCACCCG ACGTCTCGAA CTGGGTGACA ACGGTCAATT TCTTGGTGGT ATCGAGGGTA   841 AAGCTTGAGC CAGGGCCGTA GAAGCTGGTG TTGCCCAGGC GGTATGGGTT CCAGTCGCAG   901 CCATCGGGAT CGCAAGTGCC GCCATATCTG TTATCGGAGT AAGTTCCGCC GCACCCATCA   961 CCCTCGCAGA TCTCCTGGCC CGACAGTCGT GCAAGGGTGG GGGGGGTAAA GAGCCTCGGA  1021 GATGGGAGTT TGGGCCTCCC CAGATATCCC ATCTCAGAGC AAGCAGCTTT CCCGTGTCCT 1081 TCGATGGCCC GTTGATTCGC GTTTGATTGG CATGGACCGG TCTTCCCCAG ACTCT //
Primer: pGL3_RV4 Sequence file: RDB08286_A5Lac.seq
>08286_08286_A5La_pGL3_RV4_C12_09.ab1     1 GAGGGAGGTA AAGGGGGATT GAAGGCTTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA    61 ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AATCGGGAAA   121 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT   181 TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA   241 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA   301 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT   361 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG   421 CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA   481 GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA   541 TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG   601 AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT   661 GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGGTGTC   721 TGGTCAGAGA CATCAAGAAA TAACGCCGGG AAACATTAGT GCAGGCAGCT TCCACAGCAA   781 TGGGCATTCC TGGTTCATCC AGCGGATAAG TTAATGATCA GCCCACTTGA CGCGTTGCGC   841 GAAAAAGATT GGTGCACCGC CGCTTTTACA GGCTTCGACA CCGGCTTTCA TTCCTACCAT   901 TCGACACCCA CCACGCTTGG CACCCCGGTT GATTCGGGCG CGAAGAATTT TAATCCGCCG   961 CGACAAATTT GCGAACGGGC GCGTGGCGAG GGTCCAGACT GAGAGGTGGC CAACGCCCAT  1021 ATTCACCAAC CGACCTGGTT TGCCCCGGCC AGTATGTTTG GTGGCCCACC CCGGGTTTGG 1081 GGAAATAGTT AAATTTCAAG CTCTCGCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl