RDB08286 - pET21c_Tr-cbh1-His

Please review the QC test results indicated by check

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pET21c_Tr-cbh1-His (#RDB08286)

Expression vector of Exoglucanase 1 of Trichoderma reesei

Alternative name	pET21c_Tr001-His
Clone info.	Biomass collection. the Exoglucanase 1 (CBH1, 1,4-beta-cellobiohydrolase Exocellobiohydrolase I (EC 3.2.1.91)) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c.
Vector backbone	pET-21c (Plasmid)
Size of vector backbone	5441bp
Selectable markers	Amp^r
Gene/insert name	Trichoderma reesei cbh1 cDNA
Depositor\|Developer	DNA Bank, \| Kishikawa, Shotaro \|

Sequence	RDB08286z.seq
Remarks, protocol and/or map (pdf)	RDB08286.pdf

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested.

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MTAに書く使用条件	理研BRCへの謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08286	pET21c_Tr-cbh1-His	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET21c_Tr-cbh1-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08286).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08286_A5Lap1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB08286_A5Laa.seq
>08286_08286_A5La_T7_20bp_A12_03.ab1 1 TTCAAGAACC ATTTCCCCTT CTAGAATAAT TTTTGTTTAA CTTTAAGAAG GGAGATATAC 61 ATAATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGA TCCGAATTCG AGCTCCGTCG 121 ACCCAGTCGG CCTGCACTCT CCAATCGGAG ACTCACCCGC CTCTGACATG GCAGAAATGC 181 TCGTCTGGTG GCACGTGCAC TCAACAGACA GGCTCCGTGG TCATCGACGC CAACTGGCGC 241 TGGACTCACG CTACGAACAG CAGCACGAAC TGCTACGATG GCAACACTTG GAGCTCGACC 301 CTATGTCCTG ACAACGAGAC CTGCGCGAAG AACTGCTGTC TGGACGGTGC CGCCTACGCG 361 TCCACGTACG GAGTTACCAC GAGCGGTAAC AGCCTCTCCA TTGGCTTTGT CACCCAGTCT 421 GCGCAGAAGA ACGTTGGCGC TCGCCTTTAC CTTATGGCGA GCGACACGAC CTACCAGGAA 481 TTCACCCTGC TTGGCAACGA GTTCTCTTTC GATGTTGATG TTTCGCAGCT GCCGTGCGGC 541 TTGAACGGAG CTCTCTACTT CGTGTCCATG GACGCGGATG GTGGCGTGAG CAAGTATCCC 601 ACCAACACCG CTGGCGCCAA GTACGGCACG GGGTACTGTG ACAGCCAGTG TCCCCGCGAT 661 CTGAAGTTCA TCAATGGCCA GGCCAACGTT GAGGGCTGGG AGCCGTCATC CAACAACGCG 721 AACACGGGCA TTGGAGGACA CGGAAGCTGC TGCTCTGAGA TGGATATCTG GGAGGCCAAC 781 TCCATCTCCG AGGCTCTTAC CCCCCACCCT TGCACGGACT GTCGGCCAGG AGATCTGCGA 841 GGGTGATGGG GTGCGGCGGA ACTTACTCCG ATAACAGATA TGGGCGGCAC TTGCGATCCC 901 GATGGCTGCG AACTGGAACC CATACCGCCT GGGCAAACAC CAGCTTCTAC GGCCCCTGGC 961 TCAAAGCCTT TACCCTCGAA TACCACCAAG AAAATTGACC GGTTGGTCAA CCCAAGTTTC 1021 GAAGAACGTC CGGGTGCCCA TCAACCGGAT TACCTAATGT CCCAGAAATG GCGGTCACTT 1081 TTCCAGCCAG CCCCAACGCC GGAAGCTTGG TAAGTTTACT TCTTGGGCAA CGAGACCTCT 1141 CCAACACGGA ATGAGAATGT A //
Primer: f1ori_R3 Sequence file: RDB08286_A5Lab.seq
>08286_08286_A5La_f1ori_R3_B12_06.ab1 1 GCATGCATCG GGATTAGTTC CTCCTTTCAG CAAAAACCCC TCAAGACCCG TTTAGAGGCC 61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG 121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGAGTGC GGCCGCAAGC 181 TTGTCGACCA GGCACTGAGA GTAGTAAGGG TTCAGGACCT GGCAAGTTGT GCCGCTGGCG 241 CAGACCGTGG GGCCGCTGTA GCCAATACCG CCGCACTGGC CGTAGTGAGA CTGGGTAGGT 301 CCGGGAGAGC TTCCAGTGGT AGTGGCTGGG CGGCGGGTGG TGGTGGTGCC AGGCGGGTTT 361 CCGCCGGGAG GGTTGCCGCC GCTAGGGTTG CCGGTGCTGC CAATGGGTCC GAACTTGATG 421 TTGGAGAAGG TGACCTTGGC GTTGGGAGAC TGAGATTCGA CCTGAGCAGG GACACCGGAG 481 CTGGTGGAGC AGCTTCCGCG CACGGCACCG GGTGTGGAGG AGGTCTCGTT TGTCGGGTAG 541 GTGGAGTCCA GCCACAGCAT GTTGGCGTAG TAATCATCCC ACAGACTCAT GACCAGAACC 601 ATGCCGCCAG AGGTAGCCTT CTTGAACTGA GTCAGGCCGC CCTTGTCTGA GAAAGAGGAT 661 CCGCCGAATT CTGCCTCCTC AGCTGTGCAG TAATCATCGT TGAGCTCGTT GCCAGAGTAA 721 CTACCAAGCT CGGCGTTGGG CTGCTGGAAA GTGACGCCAT TCTGGACATA GTATCGGTTG 781 ATGGCACCCG ACGTCTCGAA CTGGGTGACA ACGGTCAATT TCTTGGTGGT ATCGAGGGTA 841 AAGCTTGAGC CAGGGCCGTA GAAGCTGGTG TTGCCCAGGC GGTATGGGTT CCAGTCGCAG 901 CCATCGGGAT CGCAAGTGCC GCCATATCTG TTATCGGAGT AAGTTCCGCC GCACCCATCA 961 CCCTCGCAGA TCTCCTGGCC CGACAGTCGT GCAAGGGTGG GGGGGGTAAA GAGCCTCGGA 1021 GATGGGAGTT TGGGCCTCCC CAGATATCCC ATCTCAGAGC AAGCAGCTTT CCCGTGTCCT 1081 TCGATGGCCC GTTGATTCGC GTTTGATTGG CATGGACCGG TCTTCCCCAG ACTCT //
Primer: pGL3_RV4 Sequence file: RDB08286_A5Lac.seq
>08286_08286_A5La_pGL3_RV4_C12_09.ab1 1 GAGGGAGGTA AAGGGGGATT GAAGGCTTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA 61 ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AATCGGGAAA 121 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 181 TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA 241 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA 301 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT 361 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG 421 CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA 481 GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA 541 TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG 601 AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT 661 GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGGTGTC 721 TGGTCAGAGA CATCAAGAAA TAACGCCGGG AAACATTAGT GCAGGCAGCT TCCACAGCAA 781 TGGGCATTCC TGGTTCATCC AGCGGATAAG TTAATGATCA GCCCACTTGA CGCGTTGCGC 841 GAAAAAGATT GGTGCACCGC CGCTTTTACA GGCTTCGACA CCGGCTTTCA TTCCTACCAT 901 TCGACACCCA CCACGCTTGG CACCCCGGTT GATTCGGGCG CGAAGAATTT TAATCCGCCG 961 CGACAAATTT GCGAACGGGC GCGTGGCGAG GGTCCAGACT GAGAGGTGGC CAACGCCCAT 1021 ATTCACCAAC CGACCTGGTT TGCCCCGGCC AGTATGTTTG GTGGCCCACC CCGGGTTTGG 1081 GGAAATAGTT AAATTTCAAG CTCTCGCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

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Other resources in our bank

External Database
Trichoderma reesei cbh1

Reference sequence