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pET21c_Tr-cbh1-His

Expression vector of Exoglucanase 1 of Trichoderma reesei

Catalog number RDB08286
Resource name pET21c_Tr-cbh1-His
Alternative name pET21c_Tr001-His
Clone info. Biomass collection. the Exoglucanase 1 (CBH1, 1,4-beta-cellobiohydrolase Exocellobiohydrolase I (EC 3.2.1.91)) gene, signal-peptide-less cds of Trichoderma reesei QM6a is cloned in pET21c.
Vector backbone pET-21c (Plasmid)
Size of vector backbone 5441bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei cbh1 cDNA
Depositor DNA Bank, | Kishikawa, Shotaro |

Distribution information

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RDB08286 pET21c_Tr-cbh1-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08286z.seq from Depositor
Electronic file PDF RDB08286.pdf from Depositor

Featured content

Featured content Biomass Collection (English text)
Featured content Biomass Collection (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08286_A5Lap1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08286_A5Laa.seq
>08286_08286_A5La_T7_20bp_A12_03.ab1
    1 TTCAAGAACC ATTTCCCCTT CTAGAATAAT TTTTGTTTAA CTTTAAGAAG GGAGATATAC
   61 ATAATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGA TCCGAATTCG AGCTCCGTCG
  121 ACCCAGTCGG CCTGCACTCT CCAATCGGAG ACTCACCCGC CTCTGACATG GCAGAAATGC
  181 TCGTCTGGTG GCACGTGCAC TCAACAGACA GGCTCCGTGG TCATCGACGC CAACTGGCGC
  241 TGGACTCACG CTACGAACAG CAGCACGAAC TGCTACGATG GCAACACTTG GAGCTCGACC
  301 CTATGTCCTG ACAACGAGAC CTGCGCGAAG AACTGCTGTC TGGACGGTGC CGCCTACGCG
  361 TCCACGTACG GAGTTACCAC GAGCGGTAAC AGCCTCTCCA TTGGCTTTGT CACCCAGTCT
  421 GCGCAGAAGA ACGTTGGCGC TCGCCTTTAC CTTATGGCGA GCGACACGAC CTACCAGGAA
  481 TTCACCCTGC TTGGCAACGA GTTCTCTTTC GATGTTGATG TTTCGCAGCT GCCGTGCGGC
  541 TTGAACGGAG CTCTCTACTT CGTGTCCATG GACGCGGATG GTGGCGTGAG CAAGTATCCC
  601 ACCAACACCG CTGGCGCCAA GTACGGCACG GGGTACTGTG ACAGCCAGTG TCCCCGCGAT
  661 CTGAAGTTCA TCAATGGCCA GGCCAACGTT GAGGGCTGGG AGCCGTCATC CAACAACGCG
  721 AACACGGGCA TTGGAGGACA CGGAAGCTGC TGCTCTGAGA TGGATATCTG GGAGGCCAAC
  781 TCCATCTCCG AGGCTCTTAC CCCCCACCCT TGCACGGACT GTCGGCCAGG AGATCTGCGA
  841 GGGTGATGGG GTGCGGCGGA ACTTACTCCG ATAACAGATA TGGGCGGCAC TTGCGATCCC
  901 GATGGCTGCG AACTGGAACC CATACCGCCT GGGCAAACAC CAGCTTCTAC GGCCCCTGGC
  961 TCAAAGCCTT TACCCTCGAA TACCACCAAG AAAATTGACC GGTTGGTCAA CCCAAGTTTC
 1021 GAAGAACGTC CGGGTGCCCA TCAACCGGAT TACCTAATGT CCCAGAAATG GCGGTCACTT
 1081 TTCCAGCCAG CCCCAACGCC GGAAGCTTGG TAAGTTTACT TCTTGGGCAA CGAGACCTCT
1141 CCAACACGGA ATGAGAATGT A
//
Primer: f1ori_R3 RDB08286_A5Lab.seq
>08286_08286_A5La_f1ori_R3_B12_06.ab1
    1 GCATGCATCG GGATTAGTTC CTCCTTTCAG CAAAAACCCC TCAAGACCCG TTTAGAGGCC
   61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG
  121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGAGTGC GGCCGCAAGC
  181 TTGTCGACCA GGCACTGAGA GTAGTAAGGG TTCAGGACCT GGCAAGTTGT GCCGCTGGCG
  241 CAGACCGTGG GGCCGCTGTA GCCAATACCG CCGCACTGGC CGTAGTGAGA CTGGGTAGGT
  301 CCGGGAGAGC TTCCAGTGGT AGTGGCTGGG CGGCGGGTGG TGGTGGTGCC AGGCGGGTTT
  361 CCGCCGGGAG GGTTGCCGCC GCTAGGGTTG CCGGTGCTGC CAATGGGTCC GAACTTGATG
  421 TTGGAGAAGG TGACCTTGGC GTTGGGAGAC TGAGATTCGA CCTGAGCAGG GACACCGGAG
  481 CTGGTGGAGC AGCTTCCGCG CACGGCACCG GGTGTGGAGG AGGTCTCGTT TGTCGGGTAG
  541 GTGGAGTCCA GCCACAGCAT GTTGGCGTAG TAATCATCCC ACAGACTCAT GACCAGAACC
  601 ATGCCGCCAG AGGTAGCCTT CTTGAACTGA GTCAGGCCGC CCTTGTCTGA GAAAGAGGAT
  661 CCGCCGAATT CTGCCTCCTC AGCTGTGCAG TAATCATCGT TGAGCTCGTT GCCAGAGTAA
  721 CTACCAAGCT CGGCGTTGGG CTGCTGGAAA GTGACGCCAT TCTGGACATA GTATCGGTTG
  781 ATGGCACCCG ACGTCTCGAA CTGGGTGACA ACGGTCAATT TCTTGGTGGT ATCGAGGGTA
  841 AAGCTTGAGC CAGGGCCGTA GAAGCTGGTG TTGCCCAGGC GGTATGGGTT CCAGTCGCAG
  901 CCATCGGGAT CGCAAGTGCC GCCATATCTG TTATCGGAGT AAGTTCCGCC GCACCCATCA
  961 CCCTCGCAGA TCTCCTGGCC CGACAGTCGT GCAAGGGTGG GGGGGGTAAA GAGCCTCGGA
 1021 GATGGGAGTT TGGGCCTCCC CAGATATCCC ATCTCAGAGC AAGCAGCTTT CCCGTGTCCT
1081 TCGATGGCCC GTTGATTCGC GTTTGATTGG CATGGACCGG TCTTCCCCAG ACTCT
//
Primer: pGL3_RV4 RDB08286_A5Lac.seq
>08286_08286_A5La_pGL3_RV4_C12_09.ab1
    1 GAGGGAGGTA AAGGGGGATT GAAGGCTTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA
   61 ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AATCGGGAAA
  121 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT
  181 TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA
  241 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA
  301 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT
  361 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG
  421 CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA
  481 GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA
  541 TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG
  601 AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT
  661 GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGGTGTC
  721 TGGTCAGAGA CATCAAGAAA TAACGCCGGG AAACATTAGT GCAGGCAGCT TCCACAGCAA
  781 TGGGCATTCC TGGTTCATCC AGCGGATAAG TTAATGATCA GCCCACTTGA CGCGTTGCGC
  841 GAAAAAGATT GGTGCACCGC CGCTTTTACA GGCTTCGACA CCGGCTTTCA TTCCTACCAT
  901 TCGACACCCA CCACGCTTGG CACCCCGGTT GATTCGGGCG CGAAGAATTT TAATCCGCCG
  961 CGACAAATTT GCGAACGGGC GCGTGGCGAG GGTCCAGACT GAGAGGTGGC CAACGCCCAT
 1021 ATTCACCAAC CGACCTGGTT TGCCCCGGCC AGTATGTTTG GTGGCCCACC CCGGGTTTGG
1081 GGAAATAGTT AAATTTCAAG CTCTCGCG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

GNP_filter3_RDBDEP_html_181012.pl