Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBK-CMVp62Flag (#RDB08189)

Expression vector of human p62/SQSTM1 cDNA, FLAG-tagged

Clone info. Expression vector of human p62/SQSTM1 cDNA, FLAG-tagged
Vector backbone pBK-CMV (Plasmid)
Selectable markers Kan^r
Gene/insert name Human p62 cDNA
Depositor|Developer Sudo, Tatsuhiko |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sudo, T., Biochem. Biophys. Res. Commun., 269, 521-525 (2000)) Research purpose only.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sudo, T., Biochem. Biophys. Res. Commun., 269, 521-525 (2000))。 研究目的に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08189 pBK-CMVp62Flag DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBK-CMVp62Flag was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08189).

Reference section:

Sudo, T., Maruyama, M., Osada, H., p62 functions as a p38 MAP kinase regulator. Biochem. Biophys. Res. Commun., 269, 521-525 (2000). PMID 10708586. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15186_A7Fjp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15186_A7Fja.seq check
>08189_15186_A7Fj_2_CMV-Forward_E01_05_ABI08.ab1
    1 ATTAACACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCCGC AATTACTGTG AGTTAGCTCA
  121 CTCATTAGGC ACCCCAGGCT TTACACTTTA TACTTCCGGC TCGTATATTG TGTGGAATTG
  181 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCT TGATTACGCC AAGCTCGAAA
  241 TTAACCCTCA CTAAAGGGAA CAAAAGCTGG AGCTCGCGCG CCTGCAGGTC GACACTAGTG
  301 GATCCAAAGA TTCGGCACGA GGCCAGCTCG CCGCTCGCTA TGGCGTCGCT CACCGTGAAG
  361 GCCTACCTTC TGGGCAAGGA GGACGCGGCG CGCGAGATTC GCCGCTTCAG CTTCTGCTGC
  421 AGCCCCGAGC CTGAGGCGGA AGCCGAGGCT GCGGCGGGTC CGGGACCCTG CGAGCGGCTG
  481 CTGAGCCGGG TGGCCGCCCT GTTCCCCGCG CTGCGGCCTG GCGGCTTCCA GGCGCACTAC
  541 CGCGATGAGG ACGGGGACTT GGTTGCCTTT TCCAGTGACG AGGAATTGAC AATGGCCATG
  601 TCCTACGTGA AGGATGACAT CTTCCGAATC TACATTAAAG AGAAAAAAGA GTGCCGGCGG
  661 GACCACCGCC CACCGTGTGC TCAGGAGGCG CCCCGCAACA TGGTGCACCC CAATGTGATC
  721 TGCGATGGCT GCAATGGGCC TGTGGTAGGA ACCCGCTACA AGTGCAGCGT CTGCCCAGAC
  781 TACGACTTGT GTAGCGTCTG CGAGGGAAAG GGCTTGCACC GGGGGCACAC CAAGCTCGCA
  841 TTCCCCAGCC CCTTCGGGCA CCTGTCTGAG GGCTTCTCGC ACAGCCGCTG GCTCCGGAAG
  901 GTGAAACACG GACACTTCGG GTGGCCAGGA TGGGAAATGG GTCCACCAGG AAACTGGAGC
  961 CCACGTCCTC CTCGTGCAGG GGAGGCCCGC CCTGGCCCCA CGGCAGAATC AGCTTCTGGT
 1021 CCATCGGGAG ATCCGAGTGT GAATTTCCCT GAGGACGTTG GGGAGAGTGT GGCAGCTGCC
 1081 CTTAGCCTCT GGGCATTGAA GGTTGATATC GATGTGAAGC ACGAAGGAAA GAAGGCCGGC
1141 CTTGAACCCC CGGATCCTTC TCTCCAGAAG AAAGAT
//
Primer: M13_-40
Sequence file: RDB15186_A7Fjb.seq check
>08189_15186_A7Fj_2_M13_-40_C01_03_ABI08.ab1
    1 CCGGGCCGGG TATTGTATAC GACTCACTAT AGGGCGAATT GGGTACACTT ACCTGGTACC
   61 CCACCCTCAT TTGTCGTCGT CGTCTTTATA GTCGACTATA AAGACGACGC AAATGATCAT
  121 TTGTCGTCGT CGTCTTTATA GTCGAGCGGC GGGGGATGCT TTGAATACTG GATGGTGTCC
  181 AGAGCCGCTC CGATGTCATA GTTCTTGGTC TGCAGGAGCC TGGTGAGCCA GCCGCCTTCA
  241 TCAGAGAAGC CCATGGACAG CATCTGGGAG AGGGACTCAA TCAGCCGCGG GTCAGCCTCT
  301 GGCGGGAGAT GTGGGTACAA GGCAGCTTCC TTCAGCCCTG TGGGTCCCTC CTGGGAGGGG
  361 TCCAGAGAGC TTGGCCCTTC GGATTCTGGC ATCTGTAGGG ACTGGAGTTC ACCTGTAGAC
  421 GGGTCCACTT CTTTTGAAGA CAGATGGGTC CAGTCATCAT CTCCTCCTGA ACAGTTATCC
  481 GACTCCATCT GTTCCTCAGG GCGCCCCTCG GACTCCAAGG CGATCTTCCT CATCTGCTCC
  541 GCCAGAGACT GCGTGGCGCC CTCAACATTC CCACCCGGCT TGCTGGGGTC AGAGCAGCAG
  601 CTGCTTGGCT GTGAGCTGCT CTTCTCCTCT GTGCTGGAAC TCTCTGGAGA GACGGGGGTC
  661 AGGCGGCTTC TTTTCCCTCC GTGCTCCACA TCGATATCAA CTTCAATGCC CAGAGGGCTA
  721 AGGGCAGCTG CCACACTCTC CCCAACGTTC TTCAGGAAAT TCACACTCGG ATCCTCCGAT
  781 GGACCAGAAG CTGATTCTGC CGTGGGGCCA GGGCGGGCCT CCCCTGCACG AGGAGGACGT
  841 GGGCTCCAGT TTCCTGGTGG ACCCATTTCC CATCCTGGCC ACCCGAAGTG TCCGTGTTTC
  901 ACCTTCCGGA GCCAGCGGCT GTGCGAGAAG CCCTCAGACA GGTGCCCGAA AGGGGCTGGG
  961 GAATGCGAGC TTGGTGTGCC CCCGGTGCAA GCCCTTTCCC TCGCAGACGC TACACAAGTC
 1021 GTAGTCTGGC AGACGCTGCA CTTGTAGCGG GTTCCTACCA CAGGCCCATT GCAGCCATCG
 1081 CAGATCACAT TGGGGTGCAC CATGTTGCGG GGCGCTTCTG AGCACACGGT GGGTGTTGAT
1141 TCCGCGACAC TTCTTTTATT CCTCTTTAAC TGGTAAGAAA TTCGG
//
Primer: SV40pro_F_V2
Sequence file: RDB15186_A7Fjc.seq check
>08189_15186_A7Fj_2_SV40pro_F_V2_A01_01_ABI08.ab1
    1 GGCCGAACTT TTTTTGGGGG GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG
   61 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
  121 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
  181 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
  241 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
  301 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
  361 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
  421 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
  481 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
  541 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA
  601 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
  661 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
  721 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
  781 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT
  841 ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC
  901 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  961 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
 1021 GGGAGTTCTT CGCCCACCCT TAGGGGGAGG CTAACTGAAA CACGGAAAGG AGACATACCG
 1081 GAAGGACCCC GCGCTATGAC GGCCATAAAA GACAGAATAA AACGGCACGT TGTGGGTTCG
1141 TTTGATCCAT TAAACGCGGG GTTCGGTTCC AAGGCGTGGA ACTCCTGGTT CGAATTATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sudo, T., p62 functions as a p38 MAP kinase regulator. Biochem. Biophys. Res. Commun., 269, 521-525 (2000). PMID 10708586. [link to RRC of NBRP]