Resource data sheet
Prev.

pBK-CMVp62Flag

Expression vector of human p62/SQSTM1 cDNA, FLAG-tagged

Catalog number RDB08189
Resource name pBK-CMVp62Flag
Clone info. Expression vector of human p62/SQSTM1 cDNA, FLAG-tagged
Vector backbone pBK-CMV (Plasmid)
Selectable markers Kan^r
Gene/insert name Human p62 cDNA
Depositor Sudo, Tatsuhiko |
 
Other clones in our bank human SQSTM1 (NCBI Gene 8878) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sudo, T., Biochem. Biophys. Res. Commun., 269, 521-525 (2000)) Research purpose only.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08189 pBK-CMVp62Flag DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content MAPK p38 inhibitor screening (English text)

References

original Sudo, T., p62 functions as a p38 MAP kinase regulator. Biochem. Biophys. Res. Commun., 269, 521-525 (2000). PMID 10708586.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15186_A7Fjp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB15186_A7Fja.seq
>08189_15186_A7Fj_2_CMV-Forward_E01_05_ABI08.ab1
    1 ATTAACACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCCGC AATTACTGTG AGTTAGCTCA
  121 CTCATTAGGC ACCCCAGGCT TTACACTTTA TACTTCCGGC TCGTATATTG TGTGGAATTG
  181 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCT TGATTACGCC AAGCTCGAAA
  241 TTAACCCTCA CTAAAGGGAA CAAAAGCTGG AGCTCGCGCG CCTGCAGGTC GACACTAGTG
  301 GATCCAAAGA TTCGGCACGA GGCCAGCTCG CCGCTCGCTA TGGCGTCGCT CACCGTGAAG
  361 GCCTACCTTC TGGGCAAGGA GGACGCGGCG CGCGAGATTC GCCGCTTCAG CTTCTGCTGC
  421 AGCCCCGAGC CTGAGGCGGA AGCCGAGGCT GCGGCGGGTC CGGGACCCTG CGAGCGGCTG
  481 CTGAGCCGGG TGGCCGCCCT GTTCCCCGCG CTGCGGCCTG GCGGCTTCCA GGCGCACTAC
  541 CGCGATGAGG ACGGGGACTT GGTTGCCTTT TCCAGTGACG AGGAATTGAC AATGGCCATG
  601 TCCTACGTGA AGGATGACAT CTTCCGAATC TACATTAAAG AGAAAAAAGA GTGCCGGCGG
  661 GACCACCGCC CACCGTGTGC TCAGGAGGCG CCCCGCAACA TGGTGCACCC CAATGTGATC
  721 TGCGATGGCT GCAATGGGCC TGTGGTAGGA ACCCGCTACA AGTGCAGCGT CTGCCCAGAC
  781 TACGACTTGT GTAGCGTCTG CGAGGGAAAG GGCTTGCACC GGGGGCACAC CAAGCTCGCA
  841 TTCCCCAGCC CCTTCGGGCA CCTGTCTGAG GGCTTCTCGC ACAGCCGCTG GCTCCGGAAG
  901 GTGAAACACG GACACTTCGG GTGGCCAGGA TGGGAAATGG GTCCACCAGG AAACTGGAGC
  961 CCACGTCCTC CTCGTGCAGG GGAGGCCCGC CCTGGCCCCA CGGCAGAATC AGCTTCTGGT
 1021 CCATCGGGAG ATCCGAGTGT GAATTTCCCT GAGGACGTTG GGGAGAGTGT GGCAGCTGCC
 1081 CTTAGCCTCT GGGCATTGAA GGTTGATATC GATGTGAAGC ACGAAGGAAA GAAGGCCGGC
1141 CTTGAACCCC CGGATCCTTC TCTCCAGAAG AAAGAT
//
Primer: M13_-40 RDB15186_A7Fjb.seq
>08189_15186_A7Fj_2_M13_-40_C01_03_ABI08.ab1
    1 CCGGGCCGGG TATTGTATAC GACTCACTAT AGGGCGAATT GGGTACACTT ACCTGGTACC
   61 CCACCCTCAT TTGTCGTCGT CGTCTTTATA GTCGACTATA AAGACGACGC AAATGATCAT
  121 TTGTCGTCGT CGTCTTTATA GTCGAGCGGC GGGGGATGCT TTGAATACTG GATGGTGTCC
  181 AGAGCCGCTC CGATGTCATA GTTCTTGGTC TGCAGGAGCC TGGTGAGCCA GCCGCCTTCA
  241 TCAGAGAAGC CCATGGACAG CATCTGGGAG AGGGACTCAA TCAGCCGCGG GTCAGCCTCT
  301 GGCGGGAGAT GTGGGTACAA GGCAGCTTCC TTCAGCCCTG TGGGTCCCTC CTGGGAGGGG
  361 TCCAGAGAGC TTGGCCCTTC GGATTCTGGC ATCTGTAGGG ACTGGAGTTC ACCTGTAGAC
  421 GGGTCCACTT CTTTTGAAGA CAGATGGGTC CAGTCATCAT CTCCTCCTGA ACAGTTATCC
  481 GACTCCATCT GTTCCTCAGG GCGCCCCTCG GACTCCAAGG CGATCTTCCT CATCTGCTCC
  541 GCCAGAGACT GCGTGGCGCC CTCAACATTC CCACCCGGCT TGCTGGGGTC AGAGCAGCAG
  601 CTGCTTGGCT GTGAGCTGCT CTTCTCCTCT GTGCTGGAAC TCTCTGGAGA GACGGGGGTC
  661 AGGCGGCTTC TTTTCCCTCC GTGCTCCACA TCGATATCAA CTTCAATGCC CAGAGGGCTA
  721 AGGGCAGCTG CCACACTCTC CCCAACGTTC TTCAGGAAAT TCACACTCGG ATCCTCCGAT
  781 GGACCAGAAG CTGATTCTGC CGTGGGGCCA GGGCGGGCCT CCCCTGCACG AGGAGGACGT
  841 GGGCTCCAGT TTCCTGGTGG ACCCATTTCC CATCCTGGCC ACCCGAAGTG TCCGTGTTTC
  901 ACCTTCCGGA GCCAGCGGCT GTGCGAGAAG CCCTCAGACA GGTGCCCGAA AGGGGCTGGG
  961 GAATGCGAGC TTGGTGTGCC CCCGGTGCAA GCCCTTTCCC TCGCAGACGC TACACAAGTC
 1021 GTAGTCTGGC AGACGCTGCA CTTGTAGCGG GTTCCTACCA CAGGCCCATT GCAGCCATCG
 1081 CAGATCACAT TGGGGTGCAC CATGTTGCGG GGCGCTTCTG AGCACACGGT GGGTGTTGAT
1141 TCCGCGACAC TTCTTTTATT CCTCTTTAAC TGGTAAGAAA TTCGG
//
Primer: SV40pro_F_V2 RDB15186_A7Fjc.seq
>08189_15186_A7Fj_2_SV40pro_F_V2_A01_01_ABI08.ab1
    1 GGCCGAACTT TTTTTGGGGG GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG
   61 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
  121 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
  181 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
  241 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
  301 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
  361 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
  421 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
  481 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
  541 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA
  601 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
  661 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
  721 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
  781 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT
  841 ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC
  901 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  961 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
 1021 GGGAGTTCTT CGCCCACCCT TAGGGGGAGG CTAACTGAAA CACGGAAAGG AGACATACCG
 1081 GAAGGACCCC GCGCTATGAC GGCCATAAAA GACAGAATAA AACGGCACGT TGTGGGTTCG
1141 TTTGATCCAT TAAACGCGGG GTTCGGTTCC AAGGCGTGGA ACTCCTGGTT CGAATTATC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sudo, T., p62 functions as a p38 MAP kinase regulator. Biochem. Biophys. Res. Commun., 269, 521-525 (2000). PMID 10708586.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl