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pcDNA3Flag-hExipK53A

Plasmid vector of human p38alpha MAPK splicing variant cDNA, kinase dead K53A mutant, FLAG-tagged

Catalog number RDB08185
Resource name pcDNA3Flag-hExipK53A
Clone info. Plasmid vector of human p38alpha MAPK splicing variant cDNA, kinase dead K53A mutant, FLAG-tagged.
Splicing variant form of p38alpha MAPK.
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Human p38ALPHA MAPK cDNA
Depositor Sudo, Tatsuhiko |
 
Other clones in our bank human MAPK14 (NCBI Gene 1432) |

Distribution information

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RDB08185 pcDNA3Flag-hExipK53A DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content MAPK p38 inhibitor screening (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08185_A5Ei.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB08185_A5Eia.seq
>08185_08185_A5Ei_CMV-Forwaed_C09_09.ab1
    1 TGGTACACTT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTTGCC ACCATGGACT ACAAGGACGA CGACGATAAA
  181 TCGATGTCTC AGGAGAGGCC CACGTTCTAC CGGCAGGAGC TGAACAAGAC AATCTGGGAG
  241 GTGCCCGAGC GTTACCAGAA CCTGTCTCCA GTGGGCTCTG GCGCCTATGG CTCTGTGTGT
  301 GCTGCTTTTG ACACAAAAAC GGGGTTACGT GTGGCAGTGG CGAAGCTCTC CAGACCATTT
  361 CAGTCCATCA TTCATGCGAA AAGAACCTAC AGAGAACTGC GGTTACTTAA ACATATGAAA
  421 CATGAAAATG TGATTGGTCT GTTGGACGTT TTTACACCTG CAAGGTCTCT GGAGGAATTC
  481 AATGATGTGT ATCTGGTGAC CCATCTCATG GGGGCAGATC TGAACAACAT TGTGAAATGT
  541 CAGAAGCTTA CAGATGACCA TGTTCAGTTC CTTATCTACC AAATTCTCCG AGGTCTAAAG
  601 TATATACATT CAGCTGACAT AATTCACAGG GACCTAAAAC CTAGTAATCT AGCTGTGAAT
  661 GAAGACTGTG AGCTGAAGAT TCTGGATTTT GGACTGGCTC GGCACACAGA TGATGAAATG
  721 ACAGGCTACG TGGCCACTAG GTGGTACAGG GCTCCTGAGA TCATGCTGAA CTGGATGCAT
  781 TACAACCAGA CAGTTGATAT TTGGTCAGTG GGATGCATAA TGGCCGAGCT GTTGACTGGA
  841 AGAACATTGT TTCCTGGTAC AGACCATATT GATCAGTTGA AGCTCATTTT AAGACTCGTT
  901 GGAACCCCAG GGGCTGAGCT TTTGAAGAAA ATCTCCTCAG AGTCTCTGTC GACTTGCTGG
  961 AGAAGATGCT TGTATTGGAC TCAGATAAGA GAATTACAGC GGCCCAAGCC CTTTGCACAT
 1021 GCCCTACTTT GCTCAGTACC ACGATCCTGA TGATGAACCA GTGGCCGATC CTTATGATCA
 1081 GTCCTTTGAA GCAGAACTGT ATAGATGAGT GGAAAGCCTG ACCTATGAAT GAGTCATCAG
1141 CCTTTGTGCC ACCCACCCCC CTTGGAACCA AGAAAAGAAG AAGAGTG
//
Primer: BGH_rev RDB08185_A5Eib.seq
>08185_08185_A5Ei_BGH_rev_D09_12.ab1
    1 GACCTTGCAA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT AGTGATTGTT CCCATGAGAA
   61 GGTCTTCCCC TCACAGTGAA GTGGGATCAA CAGAACAGAA ACCAGGTGCT CAGGACTCCA
  121 TCTCTTCTTG GTCAAGGGGT GGTGGCACAA AGCTGATGAC TTCATCATAG GTCAGGCTTT
  181 TCCACTCATC TATAAGGAGG TCCCTGCTTT CAAAGGACTG ATCATAAGGA TCGGCCACTG
  241 GTTCATCATC AGGATCGTGG TACTGAGCAA AGTAGGCATG TGCAAGGGCT TGGGCCGCTG
  301 TAATTCTCTT ATCTGAGTCC AATACAAGCA TCTTCTCCAG CAAGTCGACA GAGACTCTGA
  361 GGAGATTTTC TTCAAAAGCT CAGCCCCTGG GGTTCCAACG AGTCTTAAAA TGAGCTTCAA
  421 CTGATCAATA TGGTCTGTAC CAGGAAACAA TGTTCTTCCA GTCAACAGCT CGGCCATTAT
  481 GCATCCCACT GACCAAATAT CAACTGTCTG GTTGTAATGC ATCCAGTTCA GCATGATCTC
  541 AGGAGCCCTG TACCACCTAG TGGCCACGTA GCCTGTCATT TCATCATCTG TGTGCCGAGC
  601 CAGTCCAAAA TCCAGAATCT TCAGCTCACA GTCTTCATTC ACAGCTAGAT TACTAGGTTT
  661 TAGGTCCCTG TGAATTATGT CAGCTGAATG TATATACTTT AGACCTCGGA GAATTTGGTA
  721 GATAAGGAAC TGAACATGGT CATCTGTAAG CTTCTGACAT TTCACAATGT TGTTCAGATC
  781 TGCCCCCATG AGATGGGTCA CCAGATACAC ATCATTGAAT TCCTCCAGAG ACCTTGCAGG
  841 TGTAAAAACG TCCAACAGAC CAATCACATT TTCATGTTTC ATATGTTTAA GTAACCGCAG
  901 TTCTCTGTAG GTTCTTTTCG CATGAATGAT GGACTGAAAT GGTCTGGAGA GCTTCGCCAC
  961 TGCCACACGT AACCCCGTTT TTGTGTCAAA AGCAGCACAC ACAGAGCCAT AGGCGCCAGA
 1021 GCCCACTGGA GACAGGTTCT GGTAACGCTC GGGCACCTTC CCAGATTGTC TTGTCAGCTC
 1081 CTGCCCGGTA GACGTGGGCT CTCTGAGACA TCGAATTTAT CGTCGTCGTC TGGTAGTTCA
1141 ATGTGCAGGC CTTGGATCTT CTCCATTATT AGGTGGAGAG TTCGTATA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.11.21

GNP_filter3_RDBDEP_html_181012.pl