Plasmid vector of human p38alpha MAPK splicing variant cDNA, kinase dead K53A mutant, FLAG-tagged
Clone info. | Plasmid vector of human p38alpha MAPK splicing variant cDNA, kinase dead K53A mutant, FLAG-tagged. |
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Comment | Splicing variant form of p38alpha MAPK. |
Vector backbone | pcDNA3 (Plasmid) |
Selectable markers | Amp^r |
Gene/insert name | Human p38ALPHA MAPK cDNA |
Depositor|Developer | Sudo, Tatsuhiko | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Research purpose only. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 研究目的に限る。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB08185 | pcDNA3Flag-hExipK53A | DNA solution |
Materials & Methods section:
The pcDNA3Flag-hExipK53A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08185). |
Reference section:
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB08185_A5Ei.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV-Forward Sequence file: RDB08185_A5Eia.seq |
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>08185_08185_A5Ei_CMV-Forwaed_C09_09.ab1 1 TGGTACACTT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA 121 CGACTCACTA TAGGGAGACC CAAGCTTGCC ACCATGGACT ACAAGGACGA CGACGATAAA 181 TCGATGTCTC AGGAGAGGCC CACGTTCTAC CGGCAGGAGC TGAACAAGAC AATCTGGGAG 241 GTGCCCGAGC GTTACCAGAA CCTGTCTCCA GTGGGCTCTG GCGCCTATGG CTCTGTGTGT 301 GCTGCTTTTG ACACAAAAAC GGGGTTACGT GTGGCAGTGG CGAAGCTCTC CAGACCATTT 361 CAGTCCATCA TTCATGCGAA AAGAACCTAC AGAGAACTGC GGTTACTTAA ACATATGAAA 421 CATGAAAATG TGATTGGTCT GTTGGACGTT TTTACACCTG CAAGGTCTCT GGAGGAATTC 481 AATGATGTGT ATCTGGTGAC CCATCTCATG GGGGCAGATC TGAACAACAT TGTGAAATGT 541 CAGAAGCTTA CAGATGACCA TGTTCAGTTC CTTATCTACC AAATTCTCCG AGGTCTAAAG 601 TATATACATT CAGCTGACAT AATTCACAGG GACCTAAAAC CTAGTAATCT AGCTGTGAAT 661 GAAGACTGTG AGCTGAAGAT TCTGGATTTT GGACTGGCTC GGCACACAGA TGATGAAATG 721 ACAGGCTACG TGGCCACTAG GTGGTACAGG GCTCCTGAGA TCATGCTGAA CTGGATGCAT 781 TACAACCAGA CAGTTGATAT TTGGTCAGTG GGATGCATAA TGGCCGAGCT GTTGACTGGA 841 AGAACATTGT TTCCTGGTAC AGACCATATT GATCAGTTGA AGCTCATTTT AAGACTCGTT 901 GGAACCCCAG GGGCTGAGCT TTTGAAGAAA ATCTCCTCAG AGTCTCTGTC GACTTGCTGG 961 AGAAGATGCT TGTATTGGAC TCAGATAAGA GAATTACAGC GGCCCAAGCC CTTTGCACAT 1021 GCCCTACTTT GCTCAGTACC ACGATCCTGA TGATGAACCA GTGGCCGATC CTTATGATCA 1081 GTCCTTTGAA GCAGAACTGT ATAGATGAGT GGAAAGCCTG ACCTATGAAT GAGTCATCAG 1141 CCTTTGTGCC ACCCACCCCC CTTGGAACCA AGAAAAGAAG AAGAGTG // |
Primer: BGH_rev Sequence file: RDB08185_A5Eib.seq |
>08185_08185_A5Ei_BGH_rev_D09_12.ab1 1 GACCTTGCAA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT AGTGATTGTT CCCATGAGAA 61 GGTCTTCCCC TCACAGTGAA GTGGGATCAA CAGAACAGAA ACCAGGTGCT CAGGACTCCA 121 TCTCTTCTTG GTCAAGGGGT GGTGGCACAA AGCTGATGAC TTCATCATAG GTCAGGCTTT 181 TCCACTCATC TATAAGGAGG TCCCTGCTTT CAAAGGACTG ATCATAAGGA TCGGCCACTG 241 GTTCATCATC AGGATCGTGG TACTGAGCAA AGTAGGCATG TGCAAGGGCT TGGGCCGCTG 301 TAATTCTCTT ATCTGAGTCC AATACAAGCA TCTTCTCCAG CAAGTCGACA GAGACTCTGA 361 GGAGATTTTC TTCAAAAGCT CAGCCCCTGG GGTTCCAACG AGTCTTAAAA TGAGCTTCAA 421 CTGATCAATA TGGTCTGTAC CAGGAAACAA TGTTCTTCCA GTCAACAGCT CGGCCATTAT 481 GCATCCCACT GACCAAATAT CAACTGTCTG GTTGTAATGC ATCCAGTTCA GCATGATCTC 541 AGGAGCCCTG TACCACCTAG TGGCCACGTA GCCTGTCATT TCATCATCTG TGTGCCGAGC 601 CAGTCCAAAA TCCAGAATCT TCAGCTCACA GTCTTCATTC ACAGCTAGAT TACTAGGTTT 661 TAGGTCCCTG TGAATTATGT CAGCTGAATG TATATACTTT AGACCTCGGA GAATTTGGTA 721 GATAAGGAAC TGAACATGGT CATCTGTAAG CTTCTGACAT TTCACAATGT TGTTCAGATC 781 TGCCCCCATG AGATGGGTCA CCAGATACAC ATCATTGAAT TCCTCCAGAG ACCTTGCAGG 841 TGTAAAAACG TCCAACAGAC CAATCACATT TTCATGTTTC ATATGTTTAA GTAACCGCAG 901 TTCTCTGTAG GTTCTTTTCG CATGAATGAT GGACTGAAAT GGTCTGGAGA GCTTCGCCAC 961 TGCCACACGT AACCCCGTTT TTGTGTCAAA AGCAGCACAC ACAGAGCCAT AGGCGCCAGA 1021 GCCCACTGGA GACAGGTTCT GGTAACGCTC GGGCACCTTC CCAGATTGTC TTGTCAGCTC 1081 CTGCCCGGTA GACGTGGGCT CTCTGAGACA TCGAATTTAT CGTCGTCGTC TGGTAGTTCA 1141 ATGTGCAGGC CTTGGATCTT CTCCATTATT AGGTGGAGAG TTCGTATA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
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