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pGEX-hp38

Bacterial expression vector of human p38alpha MAPK cDNA, GST-tagged

Catalog number RDB08171
Resource name pGEX-hp38
Clone info. Bacterial expression vector of human p38alpha MAPK cDNA, GST-tagged.
Vector backbone pGEX (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human p38ALPHA MAPK cDNA
Depositor Sudo, Tatsuhiko |
 
Other clones in our bank human MAPK14 (NCBI Gene 1432) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Chem. Asian J., 3, 1607-1612 (2008).) Research purpose only.
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RDB08171 pGEX-hp38 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content MAPK p38 inhibitor screening (English text)

References

original Saito, A., Improvement of photoaffinity SPR imaging platform and determination of the binding site of p62/SQSTM1 to p38 MAP kinase. Chem. Asian J., 3, 1607-1612 (2008). PMID 18637653.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08171_A7Ktp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 RDB08171_A7Kta.seq
>08171_08171_A7Kt_1_pGEX-F2_B12_06_ABI24.ab1
    1 CATAAAGTTT GTTTTTAAAA CGTATTGAGC TATCCCACAA ATTGATAAGT ACTTGAAATC
   61 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA
  121 TCCTCCAAAA TCGGATCTGG TTCCGCGTGG ATCATCGATG TCTCAGGAGA GGCCCACGTT
  181 CTACCGGCAG GAGCTGAACA AGACAATCTG GGAGGTGCCC GAGCGTTACC AGAACCTGTC
  241 TCCAGTGGGC TCTGGCGCCT ATGGCTCTGT GTGTGCTGCT TTTGACACAA AAACGGGGTT
  301 ACGTGTGGCA GTGAAGAAGC TCTCCAGACC ATTTCAGTCC ATCATTCATG CGAAAAGAAC
  361 CTACAGAGAA CTGCGGTTAC TTAAACATAT GAAACATGAA AATGTGATTG GTCTGTTGGA
  421 CGTTTTTACA CCTGCAAGGT CTCTGGAGGA ATTCAATGAT GTGTATCTGG TGACCCATCT
  481 CATGGGGGCA GATCTGAACA ACATTGTGAA ATGTCAGAAG CTTACAGATG ACCATGTTCA
  541 GTTCCTTATC TACCAAATTC TCCGAGGTCT AAAGTATATA CATTCAGCTG ACATAATTCA
  601 CAGGGACCTA AAACCTAGTA ATCTAGCTGT GAATGAAGAC TGTGAGCTGA AGATTCTGGA
  661 TTTTGGACTG GCTCGGCACA CAGATGATGA AATGACAGGC TACGTGGCCA CTAGGTGGTA
  721 CAGGGCTCCT GAGATCATGC TGAACTGGAT GCATTACAAC CAGACAGTTG ATATTTGGTC
  781 AGTGGGATGC ATAATGGCCG AGCTGTTGAC TGGAAGAACA TTGTTTCCTG GTACAGACCA
  841 TATTGATCAG TTGAAGCTCA TTTTAAGACT CGTTGGAACC CCAGGGGCTG AGCTTTTGAA
  901 GAAAATCTCC TCAGAGTCTG CAAGAAACTA TATTCAGTCT TTGACTCAGA TGCCGAAGAT
  961 GAACTTTGCG AAATGTATTA TTGGTGCCCA ATCCCCCTGG CTGTCGACTT TGCTGGGAGA
 1021 AGATGCTTGT ATTGGTACTC AGGATAAGAG GAATTTAACA GCGGCCCAAG GCCCTTGGCA
1081 CATGCTACCT TGGCTTCAGT TACCACGAAT CCTGGAATGG AATGG
//
Primer: pJB8-Forward RDB08171_A7Ktb.seq
>08171_08171_A7Kt_1_pJB8-Forward_C12_09_ABI24.ab1
    1 TGGCCAATGG CTACGTGACT GGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG
   61 ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT
  121 CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGATCG
  181 TCAGTCAGTC ACGATGAATT AAGCTTGAGC TCTAGCATTT AGGTGACACT ATAGAATAGG
  241 GCCCTCTAGT GATTGTTCCC ATGAGAAGGT CTTCCCCTCA CAGTGAAGTG GGATCAACAG
  301 AACAGAAACC AGGTGCTCAG GACTCCATCT CTTCTTGGTC AAGGGGTGGT GGCACAAAGC
  361 TGATGACTTC ATCATAGGTC AGGCTTTTCC ACTCATCTAT AAGGAGGTCC CTGCTTTCAA
  421 AGGACTGATC ATAAGGATCG GCCACTGGTT CATCATCAGG ATCGTGGTAC TGAGCAAAGT
  481 AGGCATGTGC AAGGGCTTGG GCCGCTGTAA TTCTCTTATC TGAGTCCAAT ACAAGCATCT
  541 TCTCCAGCAA GTCGACAGCC AGGGGATTGG CACCAATAAA TACATTCGCA AAGTTCATCT
  601 TCGGCATCTG AGTCAAAGAC TGAATATAGT TTCTTGCAGA CTCTGAGGAG ATTTTCTTCA
  661 AAAGCTCAGC CCCTGGGGTT CCAACGAGTC TTAAAATGAG CTTCAACTGA TCAATATGGT
  721 CTGTACCAGG AAACAATGTT CTTCCAGTCA ACAGCTCGGC CATTATGCAT CCCACTGACC
  781 AAATATCAAC TGTCTGGTTG TAATGCATCC AGTTCAGCAT GATCTCAGGA GCCCTGTACC
  841 ACCTAGTGGC CACGTAGCCT GTCATTTTCA TCATCTGTGT GCCGAGCCAG TCCAAAATCC
  901 CAGAATCTTC AGCTCACAGT CTTCATTCAC AGCTAGATTA CTAGGTTTTA GGTCCCTGTG
  961 AATTATGTCA GCTGAATGTA TATACTTTTA GACCCTCGGG AGAATTTGGG TAGATTAAGG
 1021 AAACTGACAT GGTCATCTGT AAGCCTTCTG ACATTTCACA ATGATTGTTC AGATCCTGCC
1081 CCCAATGGAG AATGGGGTTC ACGAGGATTA TCA
//
Primer: pGEX-ptac_up-F RDB08171_A7Ktc.seq
>08171_08171_A7Kt_1_pGEX-ptac_up-F_D12_12_ABI24.ab1
    1 ATAACGGCGT ATCATAACGG TTCTGGCAAT ATTCTGAATG AGCTGTTGAC AATTAATCAT
   61 CGGCTCGTAT AATGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGTATTC
  121 ATGTCCCCTA TACTAGGTTA TTGGAAAATT AAGGGCCTTG TGCAACCCAC TCGACTTCTT
  181 TTGGAATATC TTGAAGAAAA ATATGAAGAG CATTTGTATG AGCGCGATGA AGGTGATAAA
  241 TGGCGAAACA AAAAGTTTGA ATTGGGTTTG GAGTTTCCCA ATCTTCCTTA TTATATTGAT
  301 GGTGATGTTA AATTAACACA GTCTATGGCC ATCATACGTT ATATAGCTGA CAAGCACAAC
  361 ATGTTGGGTG GTTGTCCAAA AGAGCGTGCA GAGATTTCAA TGCTTGAAGG AGCGGTTTTG
  421 GATATTAGAT ACGGTGTTTC GAGAATTGCA TATAGTAAAG ACTTTGAAAC TCTCAAAGTT
  481 GATTTTCTTA GCAAGCTACC TGAAATGCTG AAAATGTTCG AAGATCGTTT ATGTCATAAA
  541 ACATATTTAA ATGGTGATCA TGTAACCCAT CCTGACTTCA TGTTGTATGA CGCTCTTGAT
  601 GTTGTTTTAT ACATGGACCC AATGTGCCTG GATGCGTTCC CAAAATTAGT TTGTTTTAAA
  661 AAACGTATTG AAGCTATCCC ACAAATTGAT AAGTACTTGA AATCCAGCAA GTATATAGCA
  721 TGGCCTTTGC AGGGCTGGCA AGCCACGTTT GGTGGTGGCG ACCATCCTCC AAAATCGGAT
  781 CTGGTTCCGC GTGGATCATC GATGTCTCAG GAGAGGCCCA CGTTCTACCG GCAGGAGCTG
  841 AACAAGACAA TCTGGGAGGT GCCCGAGCGT TACCAGAACC TGTCTCCAGT GGGCTCTGGC
  901 GCCTATGGCT CTGTGTGTGC TGCTTTTGAC ACAAAAAACG GGGTTACGTG TGGCAGTGAA
  961 AGAAAGCTCT CCCAGACCCA TTTTCAGTCC ATCATTCATG CGAAAAAGAA CCCTACAGAG
 1021 AACTGCGGGT TACTTTAAAC ATATGAAAAC ATGAAAATGT TGATTTGATC TGCTTGGAAC
1081 GTTTTTACAC CTGGCCAAGG GATCTCTGGG AGGTAATTTC AAATTGA
//
Primer: M13_-40 RDB08171_A7Ktd.seq
>08171_08171_A7Kt_1_M13_-40_E12_15_ABI24.ab1
    1 CGGCACGATC GTATCATGGT CATAGCTGTT TCCTGTGTGA ATTGTTATCC GCTCACAATT
   61 CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC
  121 TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC
  181 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG
  241 GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC
  301 CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT
  361 GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC
  421 CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC
  481 CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT
  541 GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT
  601 TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT
  661 TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC
  721 CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC
  781 ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC
  841 ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC
  901 CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG
  961 TTGATCGGCG CGAGATTTAA TCGCCCGCGA CAATTTGCGA CGGGCGCGTG CAGGGCCAGG
 1021 ACTGCAGTGG CACGCCAATC AGCCACGGAC TGGTTGCTCG GCAAGCTTGG TGTGCCACGC
1081 GGATGGATGG TAATTCCAGC TCCGGACCAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Saito, A., Improvement of photoaffinity SPR imaging platform and determination of the binding site of p62/SQSTM1 to p38 MAP kinase. Chem. Asian J., 3, 1607-1612 (2008). PMID 18637653.

2018.11.21

GNP_filter3_RDBDEP_html_181012.pl