Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGEX-hp38

Bacterial expression vector of human p38alpha MAPK cDNA, GST-tagged

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Chem. Asian J., 3, 1607-1612 (2008).) Research purpose only.
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chem. Asian J., 3, 1607-1612 (2008).)。 研究目的に限る。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08171_A7Ktp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 Sequence file: RDB08171_A7Kta.seq
>08171_08171_A7Kt_1_pGEX-F2_B12_06_ABI24.ab1     1 CATAAAGTTT GTTTTTAAAA CGTATTGAGC TATCCCACAA ATTGATAAGT ACTTGAAATC    61 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA   121 TCCTCCAAAA TCGGATCTGG TTCCGCGTGG ATCATCGATG TCTCAGGAGA GGCCCACGTT   181 CTACCGGCAG GAGCTGAACA AGACAATCTG GGAGGTGCCC GAGCGTTACC AGAACCTGTC   241 TCCAGTGGGC TCTGGCGCCT ATGGCTCTGT GTGTGCTGCT TTTGACACAA AAACGGGGTT   301 ACGTGTGGCA GTGAAGAAGC TCTCCAGACC ATTTCAGTCC ATCATTCATG CGAAAAGAAC   361 CTACAGAGAA CTGCGGTTAC TTAAACATAT GAAACATGAA AATGTGATTG GTCTGTTGGA   421 CGTTTTTACA CCTGCAAGGT CTCTGGAGGA ATTCAATGAT GTGTATCTGG TGACCCATCT   481 CATGGGGGCA GATCTGAACA ACATTGTGAA ATGTCAGAAG CTTACAGATG ACCATGTTCA   541 GTTCCTTATC TACCAAATTC TCCGAGGTCT AAAGTATATA CATTCAGCTG ACATAATTCA   601 CAGGGACCTA AAACCTAGTA ATCTAGCTGT GAATGAAGAC TGTGAGCTGA AGATTCTGGA   661 TTTTGGACTG GCTCGGCACA CAGATGATGA AATGACAGGC TACGTGGCCA CTAGGTGGTA   721 CAGGGCTCCT GAGATCATGC TGAACTGGAT GCATTACAAC CAGACAGTTG ATATTTGGTC   781 AGTGGGATGC ATAATGGCCG AGCTGTTGAC TGGAAGAACA TTGTTTCCTG GTACAGACCA   841 TATTGATCAG TTGAAGCTCA TTTTAAGACT CGTTGGAACC CCAGGGGCTG AGCTTTTGAA   901 GAAAATCTCC TCAGAGTCTG CAAGAAACTA TATTCAGTCT TTGACTCAGA TGCCGAAGAT   961 GAACTTTGCG AAATGTATTA TTGGTGCCCA ATCCCCCTGG CTGTCGACTT TGCTGGGAGA  1021 AGATGCTTGT ATTGGTACTC AGGATAAGAG GAATTTAACA GCGGCCCAAG GCCCTTGGCA 1081 CATGCTACCT TGGCTTCAGT TACCACGAAT CCTGGAATGG AATGG //
Primer: pJB8-Forward Sequence file: RDB08171_A7Ktb.seq
>08171_08171_A7Kt_1_pJB8-Forward_C12_09_ABI24.ab1     1 TGGCCAATGG CTACGTGACT GGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG    61 ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT   121 CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGATCG   181 TCAGTCAGTC ACGATGAATT AAGCTTGAGC TCTAGCATTT AGGTGACACT ATAGAATAGG   241 GCCCTCTAGT GATTGTTCCC ATGAGAAGGT CTTCCCCTCA CAGTGAAGTG GGATCAACAG   301 AACAGAAACC AGGTGCTCAG GACTCCATCT CTTCTTGGTC AAGGGGTGGT GGCACAAAGC   361 TGATGACTTC ATCATAGGTC AGGCTTTTCC ACTCATCTAT AAGGAGGTCC CTGCTTTCAA   421 AGGACTGATC ATAAGGATCG GCCACTGGTT CATCATCAGG ATCGTGGTAC TGAGCAAAGT   481 AGGCATGTGC AAGGGCTTGG GCCGCTGTAA TTCTCTTATC TGAGTCCAAT ACAAGCATCT   541 TCTCCAGCAA GTCGACAGCC AGGGGATTGG CACCAATAAA TACATTCGCA AAGTTCATCT   601 TCGGCATCTG AGTCAAAGAC TGAATATAGT TTCTTGCAGA CTCTGAGGAG ATTTTCTTCA   661 AAAGCTCAGC CCCTGGGGTT CCAACGAGTC TTAAAATGAG CTTCAACTGA TCAATATGGT   721 CTGTACCAGG AAACAATGTT CTTCCAGTCA ACAGCTCGGC CATTATGCAT CCCACTGACC   781 AAATATCAAC TGTCTGGTTG TAATGCATCC AGTTCAGCAT GATCTCAGGA GCCCTGTACC   841 ACCTAGTGGC CACGTAGCCT GTCATTTTCA TCATCTGTGT GCCGAGCCAG TCCAAAATCC   901 CAGAATCTTC AGCTCACAGT CTTCATTCAC AGCTAGATTA CTAGGTTTTA GGTCCCTGTG   961 AATTATGTCA GCTGAATGTA TATACTTTTA GACCCTCGGG AGAATTTGGG TAGATTAAGG  1021 AAACTGACAT GGTCATCTGT AAGCCTTCTG ACATTTCACA ATGATTGTTC AGATCCTGCC 1081 CCCAATGGAG AATGGGGTTC ACGAGGATTA TCA //
Primer: pGEX-ptac_up-F Sequence file: RDB08171_A7Ktc.seq
>08171_08171_A7Kt_1_pGEX-ptac_up-F_D12_12_ABI24.ab1     1 ATAACGGCGT ATCATAACGG TTCTGGCAAT ATTCTGAATG AGCTGTTGAC AATTAATCAT    61 CGGCTCGTAT AATGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGTATTC   121 ATGTCCCCTA TACTAGGTTA TTGGAAAATT AAGGGCCTTG TGCAACCCAC TCGACTTCTT   181 TTGGAATATC TTGAAGAAAA ATATGAAGAG CATTTGTATG AGCGCGATGA AGGTGATAAA   241 TGGCGAAACA AAAAGTTTGA ATTGGGTTTG GAGTTTCCCA ATCTTCCTTA TTATATTGAT   301 GGTGATGTTA AATTAACACA GTCTATGGCC ATCATACGTT ATATAGCTGA CAAGCACAAC   361 ATGTTGGGTG GTTGTCCAAA AGAGCGTGCA GAGATTTCAA TGCTTGAAGG AGCGGTTTTG   421 GATATTAGAT ACGGTGTTTC GAGAATTGCA TATAGTAAAG ACTTTGAAAC TCTCAAAGTT   481 GATTTTCTTA GCAAGCTACC TGAAATGCTG AAAATGTTCG AAGATCGTTT ATGTCATAAA   541 ACATATTTAA ATGGTGATCA TGTAACCCAT CCTGACTTCA TGTTGTATGA CGCTCTTGAT   601 GTTGTTTTAT ACATGGACCC AATGTGCCTG GATGCGTTCC CAAAATTAGT TTGTTTTAAA   661 AAACGTATTG AAGCTATCCC ACAAATTGAT AAGTACTTGA AATCCAGCAA GTATATAGCA   721 TGGCCTTTGC AGGGCTGGCA AGCCACGTTT GGTGGTGGCG ACCATCCTCC AAAATCGGAT   781 CTGGTTCCGC GTGGATCATC GATGTCTCAG GAGAGGCCCA CGTTCTACCG GCAGGAGCTG   841 AACAAGACAA TCTGGGAGGT GCCCGAGCGT TACCAGAACC TGTCTCCAGT GGGCTCTGGC   901 GCCTATGGCT CTGTGTGTGC TGCTTTTGAC ACAAAAAACG GGGTTACGTG TGGCAGTGAA   961 AGAAAGCTCT CCCAGACCCA TTTTCAGTCC ATCATTCATG CGAAAAAGAA CCCTACAGAG  1021 AACTGCGGGT TACTTTAAAC ATATGAAAAC ATGAAAATGT TGATTTGATC TGCTTGGAAC 1081 GTTTTTACAC CTGGCCAAGG GATCTCTGGG AGGTAATTTC AAATTGA //
Primer: M13_-40 Sequence file: RDB08171_A7Ktd.seq
>08171_08171_A7Kt_1_M13_-40_E12_15_ABI24.ab1     1 CGGCACGATC GTATCATGGT CATAGCTGTT TCCTGTGTGA ATTGTTATCC GCTCACAATT    61 CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC   121 TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC   181 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG   241 GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC   301 CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT   361 GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC   421 CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC   481 CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT   541 GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT   601 TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT   661 TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC   721 CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC   781 ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC   841 ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC   901 CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG   961 TTGATCGGCG CGAGATTTAA TCGCCCGCGA CAATTTGCGA CGGGCGCGTG CAGGGCCAGG  1021 ACTGCAGTGG CACGCCAATC AGCCACGGAC TGGTTGCTCG GCAAGCTTGG TGTGCCACGC 1081 GGATGGATGG TAATTCCAGC TCCGGACCAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl