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pcDNA3Flag-hp38

Expression vector of human p38alpha MAPK cDNA, FLAG-tagged

Catalog number RDB08166
Resource name pcDNA3Flag-hp38
Alternative name pcDNAM2hp38
Clone info. Expression vector of human p38alpha MAPK cDNA, FLAG-tagged.
Vector backbone pcDNA3 (Plasmid)
Gene/insert name Human p38ALPHA MAPK cDNA
Depositor Sudo, Tatsuhiko |
 
Other clones in our bank human MAPK14 (NCBI Gene 1432) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Biochem. Biophys. Res. Commun., 291, 838-843 (2002).) Research purpose only.
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RDB08166 pcDNA3Flag-hp38 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content MAPK p38 inhibitor screening (English text)

References

original Sudo, T., Exip, a new alternative splicing variant of p38 alpha, can induce an earlier onset of apoptosis in HeLa cells. Biochem. Biophys. Res. Commun., 291, 838-843 (2002). PMID 11866441.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08166_A5Ei.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB08166_A5Eia.seq
>08166_08166_A5Ei_CMV-Forwaed_A09_03.ab1
    1 TGTTTCAACT TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTTGC CACCATGGAC TACAAGGACG ACGACGATAA
  181 ATCGATGTCT CAGGAGAGGC CCACGTTCTA CCGGCAGGAG CTGAACAAGA CAATCTGGGA
  241 GGTGCCCGAG CGTTACCAGA ACCTGTCTCC AGTGGGCTCT GGCGCCTATG GCTCTGTGTG
  301 TGCTGCTTTT GACACAAAAA CGGGGTTACG TGTGGCAGTG AAGAAGCTCT CCAGACCATT
  361 TCAGTCCATC ATTCATGCGA AAAGAACCTA CAGAGAACTG CGGTTACTTA AACATATGAA
  421 ACATGAAAAT GTGATTGGTC TGTTGGACGT TTTTACACCT GCAAGGTCTC TGGAGGAATT
  481 CAATGATGTG TATCTGGTGA CCCATCTCAT GGGGGCAGAT CTGAACAACA TTGTGAAATG
  541 TCAGAAGCTT ACAGATGACC ATGTTCAGTT CCTTATCTAC CAAATTCTCC GAGGTCTAAA
  601 GTATATACAT TCAGCTGACA TAATTCACAG GGACCTAAAA CCTAGTAATC TAGCTGTGAA
  661 TGAAGACTGT GAGCTGAAGA TTCTGGATTT TGGACTGGCT CGGCACACAG ATGATGAAAT
  721 GACAGGCTAC GTGGCCACTA GGTGGTACAG GGCTCCTGAG ATCATGCTGA ACTGGATGCA
  781 TTACAACCAG ACAGTTGATA TTTGGTCAGT GGGATGCATA ATGGCCGAGC TGTTGACTGG
  841 AAGAACATTG TTTCCTGGTA CAGACCATAT TGATCAGTTG AAGCTCATTT TAAGACTCGT
  901 TGGAACCCCA GGGGCTGAGC TTTTGAAGAA AATCTCCTCA GAGTCTGCAA GAAACTATAT
  961 TCAGTCTTTG ACTCAGATGC CGAAGATGAA CTTTGCGAAT GTATTATTGG TGCCAATCCC
 1021 CTGGCTGTCG ACTTGCTGGA GAGATGCTTG TATGACTCAG ATAGAGATTA CAGCGGGCCC
 1081 AAGCCCTGCA CATGCTACTT TGCTCAGTAC CACGATCTGA ATGATGATAA CTGCGGATCT
1141 ATGAATCAGT CCCTTTGAAA AGGCCAGGG
//
Primer: BGH_rev RDB08166_A5Eib.seq
>08166_08166_A5Ei_BGH_rev_B09_06.ab1
    1 GGTCTTTCAT TTAGGTGACC TATAGAATAG GGCCCTCTAG TGATTGTTCC CATGAGAAGG
   61 TCTTCCCCTC ACAGTGAAGT GGGATCAACA GAACAGAAAC CAGGTGCTCA GGACTCCATC
  121 TCTTCTTGGT CAAGGGGTGG TGGCACAAAG CTGATGACTT CATCATAGGT CAGGCTTTTC
  181 CACTCATCTA TAAGGAGGTC CCTGCTTTCA AAGGACTGAT CATAAGGATC GGCCACTGGT
  241 TCATCATCAG GATCGTGGTA CTGAGCAAAG TAGGCATGTG CAAGGGCTTG GGCCGCTGTA
  301 ATTCTCTTAT CTGAGTCCAA TACAAGCATC TTCTCCAGCA AGTCGACAGC CAGGGGATTG
  361 GCACCAATAA ATACATTCGC AAAGTTCATC TTCGGCATCT GAGTCAAAGA CTGAATATAG
  421 TTTCTTGCAG ACTCTGAGGA GATTTTCTTC AAAAGCTCAG CCCCTGGGGT TCCAACGAGT
  481 CTTAAAATGA GCTTCAACTG ATCAATATGG TCTGTACCAG GAAACAATGT TCTTCCAGTC
  541 AACAGCTCGG CCATTATGCA TCCCACTGAC CAAATATCAA CTGTCTGGTT GTAATGCATC
  601 CAGTTCAGCA TGATCTCAGG AGCCCTGTAC CACCTAGTGG CCACGTAGCC TGTCATTTCA
  661 TCATCTGTGT GCCGAGCCAG TCCAAAATCC AGAATCTTCA GCTCACAGTC TTCATTCACA
  721 GCTAGATTAC TAGGTTTTAG GTCCCTGTGA ATTATGTCAG CTGAATGTAT ATACTTTAGA
  781 CCTCGGAGAA TTTGGTAGAT AAGGAACTGA ACATGGTCAT CTGTAAGCTT CTGACATTTC
  841 ACAATGTTGT TCAGATCTGC CCCCATGAGA TGGGTCACCA GATACACATC ATTGAATTCC
  901 TCCAGAGACC TTGCAGGTGT AAAAACGTCC AACAGACCAA TCACATTTTC ATGTTTCATA
  961 TGTTTAAGTA ACCGCAGTTC TCTGTAGGTT CTTTTCGCAT GAATGATGGG ACTGAAATGG
 1021 TCTGGAGAGC TTCTTCACTG CCACCACGTA ACCCCGTTTT GTGTCAAAAG CAGCACACAC
 1081 AGGAGTCATA GCGCAGAGCC CACTGGAGAA CAGGTCTGTA ACGCTCGGGC ACCTCCAGAT
1141 TGTCTTGTTC AGCCTCCTTA CCGGTTAGAC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sudo, T., Exip, a new alternative splicing variant of p38 alpha, can induce an earlier onset of apoptosis in HeLa cells. Biochem. Biophys. Res. Commun., 291, 838-843 (2002). PMID 11866441.

2018.11.21

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