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pSRalpha3HA-p38

Expression vector of mouse p38alpha MAPK cDNA, HA-tagged

Catalog number RDB08163
Resource name pSRalpha3HA-p38
Clone info. Expression vector of mouse p38alpha MAPK cDNA, HA-tagged.
Vector backbone pSRa3HA (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse p38alpha MAPK cDNA
Depositor Sudo, Tatsuhiko |
 
Other clones in our bank mouse Mapk14 (NCBI Gene 26416) |

Distribution information

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RDB08163 pSRalpha3HA-p38 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content MAPK p38 inhibitor screening (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08163_A5G1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pro_F_V2 RDB08163_A5G1a.seq
>08163_08163_A5G1_SV40pro_F_V2_D12_12.ab1
    1 CTACGGGTTT TTTGGAGCTA GGCTTTTGCA AAAGCTCCTC GATCGAGGGG CTCGCATCTC
   61 TCCTTCACGC GCCCGCCGCC CTACCTGAGG CCGCCATCCA CGCCGGTTGA GTCGCGTTCT
  121 GCCGCCTCCC GCCTGTGGTG CCTCCTGAAC TGCGTCCGCC GTCTAGGTAA GTTTAAAGCT
  181 CAGGTCGAGA CCGGGCCTTT GTCCGGCGCT CCCTTGGAGC CTACCTAGAC TCAGCCGGCT
  241 CTCCACGCTT TGCCTGACCC TGCTTGCTCA ACTCTACGTC TTTGTTTCGT TTTCTGTTCT
  301 GCGCCGTTAC AGATCCAAGC TCCTCGAGGA ACTGAAAAAC CAGAAAGTTA ACTGGTAAGT
  361 TTAGTCTTTT TGTCTTTTAT TTCAGGTCCC GGATCCGGTG GTGGTGCAAA TCAAAGAACT
  421 GCTCCTCAGT GGATGTTGCC TTTACTTCTA GGCCTGTACG GAAGTGTTAC TTCTGCTCTA
  481 AAAGCTGCGG AATTCATATT GTCGTTAGAA CGCGGCTACA ATTAATACAT AACCTTATGT
  541 ATCATACACA TACGATTTAG GTGACACTAT AGAATACAAG CTTGCTTGTT CTTTTTGCAG
  601 AAGCTCAGAA TAAACGCTCA ACTTTGGCCA TGTACCCATA CGATGTTCCA GATTACGCCA
  661 TGTACCCATA CGATGTTCCA GATTACGCCA TGTACCCATA CGATGTTCCA GATTACGCCA
  721 TGGCAAATGT CGCAGGAGAG GCCCACGTTC TACCGGCAGG AGCTGAACAA GACCATCTGG
  781 GAGGTGCCCG AACGATACCA GAACCTGTCC CCGGTGGGCT CGGGCGCCTA TGGCTCGGTG
  841 TGTGCTGCTT TTGATACAAA GACGGGGGCA TCGTGTGGCA GTTAAGAAGC TGTCGAGACC
  901 GTTTCAGTCC ATCATTCAAC GCCAAAAGGG ACCTACCGAG AGTTGCGTCT GCTGAAAGCA
  961 CATGAAACAC GAAAAATGTG ATTGGGTCTG TTGGATGTGT TTCACACCCC GGCAAGGTCA
 1021 CTGGAAGGAA TTTCAATGGA CCGTGTTACC CTTGGTTGAC CCATCTTCAT GGGGGGCGGA
 1081 ACCCTTGAAC ACAATTCGTT GAAGTTGCCC AGAAAGCCTG GACCGGACGA ACCACGGTTT
1141 CCAGTTTTCC TCAATCCTTA CCAGAAAATT CCCTCCTCGA GAAAGGGGGC CTGGA
//
Primer: SP6 RDB08163_A5G1b.seq
>08163_08163_A5G1_Sp6_A12_03.ab1
    1 GTGGGGATTT TTTTGCAGAG CTCAGATAAA CGCTCAACTT TGGCCATGTA CCCATACGAT
   61 GTTCCAGATT ACGCCATGTA CCCATACGAT GTTCCAGATT ACGCCATGTA CCCATACGAT
  121 GTTCCAGATT ACGCCATGGC AATGTCGCAG GAGAGGCCCA CGTTCTACCG GCAGGAGCTG
  181 AACAAGACCA TCTGGGAGGT GCCCGAACGA TACCAGAACC TGTCCCCGGT GGGCTCGGGC
  241 GCCTATGGCT CGGTGTGTGC TGCTTTTGAT ACAAAGACGG GGCATCGTGT GGCAGTTAAG
  301 AAGCTGTCGA GACCGTTTCA GTCCATCATT CACGCCAAAA GGACCTACCG AGAGTTGCGT
  361 CTGCTGAAGC ACATGAAACA CGAAAATGTG ATTGGTCTGT TGGATGTGTT CACACCCGCA
  421 AGGTCACTGG AGGAATTCAA TGACGTGTAC CTGGTGACCC ATCTCATGGG GGCGGACCTG
  481 AACAACATCG TGAAGTGCCA GAAGCTGACC GACGACCACG TTCAGTTTCT CATCTACCAG
  541 ATCCTCCGAG GGCTGAAGTA TATACATTCG GCTGACATAA TTCACAGGGA CCTAAAGCCC
  601 AGCAACCTAG CTGTGAACGA AGACTGTGAG CTCAAGATTC TGGATTTTGG GCTGGCTCGG
  661 CACACTGATG ATGAGATGAC AGGCTACGTG GCTACCAGGT GGTACCGAGC CCCAGAGATC
  721 ATGCTGAATT GGATGCACTA TAACCAGACA GTGGATATTT GGTCCGTGGG CTGCATCATG
  781 GCTGAGCTGT TGACCGGAAG AACGTTGTTT CCTGGTACAG ACCATATTGA TCAGTTGAAG
  841 CTCATTTTAA GACTCGTTGG AACCCCAGGG GCTGAGCTTC TGAAGAAAAT CTCCTCAGAG
  901 TCTGCAAGAA ACTACATTCA GTCTCTGGCC CAGATGCCGA AGATGAACTT CGCAAATGTA
  961 TTTATTGGTG CCAATCCCCT GGCTGTCGAC CTACTGGAGA AGATGCTCGT TTTGGGACTC
 1021 AGATAAGAGG GATCACAGCA GCCCAAGCTC TTGCGCATGC CCTACTTTGC TTCAGTAACA
 1081 ACGACCCTGA TGATGAGTCT GCTGCCTGAA CCATTAATGG AACAAGGTCC GTTTGCAAAA
1141 GTCAGGACCT TTCTCA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.11.21

GNP_filter3_RDBDEP_html_181012.pl