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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-CMV-Venus-Mito-IRES2-Bsd

Lentivirus vector plasmid of Venus fluorescent protein with mitochondria localization signal driven by CMV promoter.

Catalog number RDB08118
Resource name CSII-CMV-Venus-Mito-IRES2-Bsd
Clone info. Lentivirus vector plasmid of Venus fluorescent protein with mitochondria localization signal driven by CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            Please contact us. We will provide you with appropriate documents.
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            付加的提供条件 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            宮脇敦史博士による提供承諾書 [Word]
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08118 CSII-CMV-Venus-Mito-IRES2-Bsd DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB08118z.seq
            Remarks, protocol and/or map (pdf) RDB08118.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08118_A3Ihp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Venus-N_R
            Sequence file: RDB08118_A3Iha.seq check
            >08118_08118_A3Ih_Venus-N_R_D09_12.ab1
                1 CCGTCTGAGC TGACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA
               61 CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTGGC GACCGGTGGA TCCCCCAACG
              121 AATGGATCTT GGCGCGCGGC ACTGGGAGCC GCCGGGCCGA GCCTGTCAAG CCCCGCAGCA
              181 GCAGCGGCGT CAGGACGGAC ATGCTAGCGG ATCTGACGGT TCACTAAACC AGCTCTGCTT
              241 ATATAGACCT CCCACCGTAC ACGCCTACCG CCCATTTGCG TCAATGGGGC GGAGTTGTTA
              301 CGACATTTTG GAAAGTCCCG TTGATTTTGG TGCCAAAACA AACTCCCATT GACGTCAATG
              361 GGGTGGAGAC TTGGAAATCC CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC
              421 CAAAACCGCA TCACCATGGT AATAGCGATG ACTAATACGT AGATGTACTG CCAAGTAGGA
              481 AAGTCCCATA AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC GTCATTGACG
              541 TCAATAGGGG GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCAAGTG GGCAGTTTAC
              601 CGTAAATACT CCACCCATTG ACGTCAATGG AAAGTCCCTA TTGGCGTTAC TATGGGAACA
              661 TACGTCATTA TTGACGTCAA TGGGCGGGGG TCGTTGGGCG GTCAGCCAGG CGGGCCATTT
              721 ACCGTAAGTT ATGTAACGCG GAACTCCATA TATGGGCTAT GAACTAATGA CCCCGTAATT
              781 GATTACTATT AATCGAATTA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA TAAACCCGAA
              841 AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT GTTGCTATTA
              901 TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT
              961 GGATGAATAC TGCCATTTGT AGATCCGTTT CACTAATCGA ATGGATCTGT CTCTGTCTCT
             1021 CTCTCACTTT CCTCCTCTAT TCTTCCGGCT GTCAGTCCCT CGGGGTTTGG AGGTGGGTCT
            1081 GAATCGATTA TGATGATATC CATGGCTACT TCTTATTTCA CTATTAGG
            //
            Primer: IRES-R
            Sequence file: RDB08118_A3Ihb.seq check
            >08118_08118_A3Ih_IRES-R_D10_10.ab1
                1 GCACGGGGGG CTTCGGGCCA GTACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG
               61 CCGCTGCAGA ATTCTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC
              121 ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC
              181 TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC
              241 TGCTGGTAGT GGTCGGCGAG CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG
              301 TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG
              361 TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC
              421 TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG
              481 CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG
              541 AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCC
              601 AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC
              661 AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG
              721 CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG
              781 CCCTTGCTCA CCATGGTGGC GACCGGTGGA TCCCCCAACG AATGGATCTT GGCGCGCGGC
              841 ACTGGGGAGC CGCCGGGCCG AGCCTGTCAA GCCCCGCAGC AGCAGCGGCG TCAGGACGGA
              901 CATGCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT TATATAGACC TCCCACCGTA
              961 CACGCCCTAC CGCCCATTTG CGTCAATGGG GGCGGAGTTG TTACGACATT TTTGGGAAAG
             1021 TCCCGTTTGA TTTTTGGTGC CAAACAAACT CCATGACGTC ATGGGGTGGA GACTTGGAAT
            1081 TCCCCGTTGA GTCAACGGCC TATTCAACGC CCCCAA
            //
            Primer: IRES-F
            Sequence file: RDB08118_A3Ihc.seq check
            >08118_08118_A3Ih_IRES-F_H08_23.ab1
                1 GCATCACTTC ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
               61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGCC AAGCCTTTGT
              121 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT
              181 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG
              241 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG
              301 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA
              361 TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG
              421 CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT
              481 CTGGTTATGT GTGGGAGGGC TAAGTCGAGC CTCTAGAGTT AACATCGAGG GATCAAGCTT
              541 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT
              601 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT
              661 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG
              721 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC
              781 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC
              841 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT
              901 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG
              961 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG
             1021 GCCCTCAATC CAGCGGGACC TTCTTCCCGC GGCCTGCTGC CGGCTCTGCG GGCTCTTCGC
             1081 GTCTTCGCTT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTT GGGCCGCCTC CCCGCAATCG
             1141 ATACGGTCGA GACTTAGAAA AACATGAAGC AATCACAAGT TAGCCAAATA ACAGCAGAGC
            1201 CTTAACCTAA T
            //
            Primer: HIVgp_R1
            Sequence file: RDB08118_A3Ihd.seq check
            >08118_08118_A3Ih_HIVgp_R1_E10_13.ab1
                1 CGGGACCTCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
               61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
              121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
              181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
              241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
              301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
              361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
              421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
              481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
              541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
              601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
              661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT
              721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
              781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG
              841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA
              901 CACGCCTACC GCCCATTTGC GTCAATGGGG GCGGGAGTTG TTACGACATT TTGGAAAGTC
              961 CCGTTTGATT TTGGGTGCCA AAACAAACTC CCATTGACGT CATGGGGGTG GGAGACTTGG
             1021 GAAATCCCCG TGAGTCAAAC CGCTAATCCA CGCTCATGAT GTTACTGCCA AACCGGCATC
            1081 ACAATGCTAA TAGCGAATGA CTTAATTAAC GCTAGATGGT AC
            //
            Primer: BGH_rev2
            Sequence file: RDB08118_A3Ihe.seq check
            >08118_08118_A3Ih_BGH_rev2_F10_16.ab1
                1 CCACTCCCCC GATCAGTAAG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
               61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA
              121 CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC
              181 TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA
              241 GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC
              301 TCGGGCCATG TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC
              361 CGGGTCATCC ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT
              421 GGCTTCTTCT ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG
              481 TGGATATCTG ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT
              541 GTGGTAGATC CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG
              601 TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT
              661 TAAAGGTACC TGAGGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA
              721 GGCACAATCA GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG
              781 GTCTCGACGG TATCGATGCG GGGAGGCGGC CCAAAGGGAG ATCCGACTCG TCTGAGGGCG
              841 AAGGCGAAGA CGCGGAAGAG GCCGCAGAGC CGGCAGCAGG CCGCGGGAAA GGAAAGGTCC
              901 GCTGGATTGA GGGCCGACGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA
              961 ACACAGGCGA GCAGCCATGG GAAAGGACGT CAGCTTCCCC CGACAACACC ACGGGAATTT
             1021 GTCAGTTGCC TCAACAGCCC GGAGCCCCCT GTCAGCCAGC CGGGCAAGCA AGCGGCGATG
            1081 AGTCATCGAT GACATTAGGG AGGGGAAAGC CGAAAAGT
            //
            Primer: SV40pro_F
            Sequence file: RDB08118_A3Ihf.seq check
            >08118_08118_A3Ih_SV40pro_F_G10_19.ab1
                1 CTTTGGTCTG TGGCTTTTCA GGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
               61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAGCACG TGTTGACAAT
              121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG
              181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
              241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
              301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
              361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
              421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
              481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
              541 GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
              601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
              661 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
              721 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
              781 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG
              841 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAAATTGT TATCCGCTCA CAATTCCACA
              901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AAGCCTGGGG GTGCCTAATG AGTGAGCTAA
              961 CTCACATTTA ATTGCGTTTG CGCTCACTGC CCGGCTTTCC AGTCGGGAAA ACCTGTCGTG
             1021 CCAAGCTGCA TTTAATGAAT CGGCCAACGG CGCGGAAGAG GCGGGTTTGC GTATTGGGCG
            1081 CTCTTTCGGC CTATCCATCG CTCACCACTG GGACATTCG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Ogawa, Y., Elavl3 is essential for the maintenance of Purkinje neuron axons. Sci. Rep. 8 (1): 2722 (2018). PMID 29426875.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl