Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-CMV-Venus-Mito-IRES2-Bsd

Lentivirus vector plasmid of Venus fluorescent protein with mitochondria localization signal driven by CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08118_A3Ihp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Venus-N_R Sequence file: RDB08118_A3Iha.seq
>08118_08118_A3Ih_Venus-N_R_D09_12.ab1     1 CCGTCTGAGC TGACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA    61 CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTGGC GACCGGTGGA TCCCCCAACG   121 AATGGATCTT GGCGCGCGGC ACTGGGAGCC GCCGGGCCGA GCCTGTCAAG CCCCGCAGCA   181 GCAGCGGCGT CAGGACGGAC ATGCTAGCGG ATCTGACGGT TCACTAAACC AGCTCTGCTT   241 ATATAGACCT CCCACCGTAC ACGCCTACCG CCCATTTGCG TCAATGGGGC GGAGTTGTTA   301 CGACATTTTG GAAAGTCCCG TTGATTTTGG TGCCAAAACA AACTCCCATT GACGTCAATG   361 GGGTGGAGAC TTGGAAATCC CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC   421 CAAAACCGCA TCACCATGGT AATAGCGATG ACTAATACGT AGATGTACTG CCAAGTAGGA   481 AAGTCCCATA AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC GTCATTGACG   541 TCAATAGGGG GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCAAGTG GGCAGTTTAC   601 CGTAAATACT CCACCCATTG ACGTCAATGG AAAGTCCCTA TTGGCGTTAC TATGGGAACA   661 TACGTCATTA TTGACGTCAA TGGGCGGGGG TCGTTGGGCG GTCAGCCAGG CGGGCCATTT   721 ACCGTAAGTT ATGTAACGCG GAACTCCATA TATGGGCTAT GAACTAATGA CCCCGTAATT   781 GATTACTATT AATCGAATTA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA TAAACCCGAA   841 AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT GTTGCTATTA   901 TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT   961 GGATGAATAC TGCCATTTGT AGATCCGTTT CACTAATCGA ATGGATCTGT CTCTGTCTCT  1021 CTCTCACTTT CCTCCTCTAT TCTTCCGGCT GTCAGTCCCT CGGGGTTTGG AGGTGGGTCT 1081 GAATCGATTA TGATGATATC CATGGCTACT TCTTATTTCA CTATTAGG //
Primer: IRES-R Sequence file: RDB08118_A3Ihb.seq
>08118_08118_A3Ih_IRES-R_D10_10.ab1     1 GCACGGGGGG CTTCGGGCCA GTACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG    61 CCGCTGCAGA ATTCTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC   121 ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC   181 TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC   241 TGCTGGTAGT GGTCGGCGAG CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG   301 TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG   361 TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC   421 TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG   481 CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG   541 AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCC   601 AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC   661 AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG   721 CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG   781 CCCTTGCTCA CCATGGTGGC GACCGGTGGA TCCCCCAACG AATGGATCTT GGCGCGCGGC   841 ACTGGGGAGC CGCCGGGCCG AGCCTGTCAA GCCCCGCAGC AGCAGCGGCG TCAGGACGGA   901 CATGCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT TATATAGACC TCCCACCGTA   961 CACGCCCTAC CGCCCATTTG CGTCAATGGG GGCGGAGTTG TTACGACATT TTTGGGAAAG  1021 TCCCGTTTGA TTTTTGGTGC CAAACAAACT CCATGACGTC ATGGGGTGGA GACTTGGAAT 1081 TCCCCGTTGA GTCAACGGCC TATTCAACGC CCCCAA //
Primer: IRES-F Sequence file: RDB08118_A3Ihc.seq
>08118_08118_A3Ih_IRES-F_H08_23.ab1     1 GCATCACTTC ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA    61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGCC AAGCCTTTGT   121 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT   181 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG   241 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG   301 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA   361 TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG   421 CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT   481 CTGGTTATGT GTGGGAGGGC TAAGTCGAGC CTCTAGAGTT AACATCGAGG GATCAAGCTT   541 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT   601 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT   661 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG   721 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC   781 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC   841 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT   901 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG   961 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG  1021 GCCCTCAATC CAGCGGGACC TTCTTCCCGC GGCCTGCTGC CGGCTCTGCG GGCTCTTCGC  1081 GTCTTCGCTT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTT GGGCCGCCTC CCCGCAATCG  1141 ATACGGTCGA GACTTAGAAA AACATGAAGC AATCACAAGT TAGCCAAATA ACAGCAGAGC 1201 CTTAACCTAA T //
Primer: HIVgp_R1 Sequence file: RDB08118_A3Ihd.seq
>08118_08118_A3Ih_HIVgp_R1_E10_13.ab1     1 CGGGACCTCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG    61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA   121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG   181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA   241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT   301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG   361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA   421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG   481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC   541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG   601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT   661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT   721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT   781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG   841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA   901 CACGCCTACC GCCCATTTGC GTCAATGGGG GCGGGAGTTG TTACGACATT TTGGAAAGTC   961 CCGTTTGATT TTGGGTGCCA AAACAAACTC CCATTGACGT CATGGGGGTG GGAGACTTGG  1021 GAAATCCCCG TGAGTCAAAC CGCTAATCCA CGCTCATGAT GTTACTGCCA AACCGGCATC 1081 ACAATGCTAA TAGCGAATGA CTTAATTAAC GCTAGATGGT AC //
Primer: BGH_rev2 Sequence file: RDB08118_A3Ihe.seq
>08118_08118_A3Ih_BGH_rev2_F10_16.ab1     1 CCACTCCCCC GATCAGTAAG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA    61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA   121 CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC   181 TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA   241 GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC   301 TCGGGCCATG TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC   361 CGGGTCATCC ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT   421 GGCTTCTTCT ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG   481 TGGATATCTG ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT   541 GTGGTAGATC CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG   601 TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT   661 TAAAGGTACC TGAGGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA   721 GGCACAATCA GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG   781 GTCTCGACGG TATCGATGCG GGGAGGCGGC CCAAAGGGAG ATCCGACTCG TCTGAGGGCG   841 AAGGCGAAGA CGCGGAAGAG GCCGCAGAGC CGGCAGCAGG CCGCGGGAAA GGAAAGGTCC   901 GCTGGATTGA GGGCCGACGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA   961 ACACAGGCGA GCAGCCATGG GAAAGGACGT CAGCTTCCCC CGACAACACC ACGGGAATTT  1021 GTCAGTTGCC TCAACAGCCC GGAGCCCCCT GTCAGCCAGC CGGGCAAGCA AGCGGCGATG 1081 AGTCATCGAT GACATTAGGG AGGGGAAAGC CGAAAAGT //
Primer: SV40pro_F Sequence file: RDB08118_A3Ihf.seq
>08118_08118_A3Ih_SV40pro_F_G10_19.ab1     1 CTTTGGTCTG TGGCTTTTCA GGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG    61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAGCACG TGTTGACAAT   121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG   181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG   241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG   301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC   361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC   421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG   481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG   541 GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG   601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG   661 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC   721 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG   781 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG   841 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAAATTGT TATCCGCTCA CAATTCCACA   901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AAGCCTGGGG GTGCCTAATG AGTGAGCTAA   961 CTCACATTTA ATTGCGTTTG CGCTCACTGC CCGGCTTTCC AGTCGGGAAA ACCTGTCGTG  1021 CCAAGCTGCA TTTAATGAAT CGGCCAACGG CGCGGAAGAG GCGGGTTTGC GTATTGGGCG 1081 CTCTTTCGGC CTATCCATCG CTCACCACTG GGACATTCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl