Resource data sheet
Prev.

pmmCasp8 (Mouse caspase-8)

Plasmid clone of mouse caspase-8 cDNA

Catalog number RDB08071
Resource name pmmCasp8 (Mouse caspase-8)
Alternative name pBS-MmCasp8
Clone info. Plasmid clone of mouse caspase-8 cDNA.
Vector backbone pBluescript SK (Plasmid)
Size of vector backbone 3,204 bp
Selectable markers Amp^r
Gene/insert name Mouse caspase 8 cDNA
Reference of nucleotide sequence AF067834 (DDBJ accession number)
Depositor Sakamaki, Kazuhiro |
 
Other clones in our bank mouse Casp8 (NCBI Gene 12370) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08071 pmmCasp8 (Mouse caspase-8) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Sakamaki, K., Molecular cloning and characterization of mouse caspase-8. Eur. J. Biochem., 253, 399-405 (1998). PMID 9654089.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08071_A6Ikp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB08071_A6Ika.seq
>08071_08071_A6Ik_M13(-40)_B11_05_ABI24.ab1
    1 CCTCTGCATG TATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC CCTCGAGGTC
   61 GACGGTATCG ATAAGCTTGA TATCGAATTC GGCACGAGGC TAAACATTCG GAGGCATTTC
  121 TGTCCCCTAT GCCCTAGTTC TCTCAGTTGT CTTTCATTCT GACTTCGGTG CTTAAAAGTC
  181 CAGCTTCTCG GAATCGGTAG CAAACCTCTG TGAGCCGGCG TGGAACAGGA AGTGAGTACA
  241 GTTCTGGGGA GCGCCGGCCC GGGCTGGAGG CTCGGAAAGC CCAAGCCAGC GGAGGCCTCG
  301 CCCGAGCTGG AGTTGTGACC GGCGCGGCAG GTACTCGGCC ACAGGTTACA GCTCTTCTAC
  361 CTCTTGATAA GAATGGATTT CCAGAGTTGT CTTTATGCTA TTGCTGAAGA ACTGGGCAGT
  421 GAAGACCTGG CTGCCCTCAA GTTCCTGTGC TTGGACTACA TCCCACACAA GAAGCAGGAG
  481 ACCATCGAGG ATGCCCAGAA GCTATTTCTG AGGCTGCGGG AAAAGGGGAT GTTGGAGGAA
  541 GGCAATCTGT CTTTCCTGAA AGAGCTGCTT TTCCACATCA GTCGGTGGGA CCTGCTGGTC
  601 AACTTCCTAG ACTGCAACCG AGAGGAGATG GTGAGAGAGC TGCGGGATCC AGACAATGCC
  661 CAGATTTCTC CCTACAGGGT CATGCTCTTT AAGCTCTCAG AAGAAGTGAG CGAGTTGGAA
  721 TTGAGATCTT TTAAGTTCCT TTTGAACAAT GAGATCCCCA AATGTAAGCT GGAAGATGAC
  781 TTGAGCCTGC TTGAAATTTT TGTAGAAATG GAGAAGAGGA CCATGCTGGC AGAAAATAAC
  841 TTGGAAACCC TAAAATCAAT CTGTGACCAG GTCAACAAGA GCCTGCTGGG GGAAGATCGA
  901 GGATTATGAA AGATCAAGCA CAGAGAGAAG AATGAGCCTT GAAGGAAGGG AAGAGTTGCC
  961 ACCTTCAGTT TTGGATGAGA TGAGCCCTCA AATGGCGGGA CTGTGTGACT CGCGAGGAGA
 1021 CAGGACAGGT GAGGTCACGA ACTCAGAACG AAGTTTAACA AATGCAGATC GAATCCTTCC
1081 GGGGGCATTA CCTGATCTTG GAATCATTCC A
//
Primer: Reverse2 RDB08071_A6Ikb.seq
>08071_08071_A6Ik_Reverse2_C11_08_ABI24.ab1
    1 CGTAATCATT ACGCCAGCTC GAATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTCCA
   61 CCGCGGTGGC GGCCGCAGGA ATTTTTTTTT TTTTTTTTTT TACAAGTGAT AGTATACAGG
  121 TTATATTTAG AAATGTGTGT GTGTGTGTGT GTGTGTGTAT GTATGTGTGT GTACAACAGT
  181 TAATGAGAAA AGAGGCCATG TATTTGAAAT TGGAACATAA CAAAGAAGGG TTTAAAGGGA
  241 AGAAAAGGGA AAATTATGTA AATGTAAATT CAAAAGCAAA AGAAATTCTA AAAAATAAAT
  301 TAGACTCATC CACACAAAAG TGCACAGATA AGCCATGTGA ACTGTGGAGA GCACACATCA
  361 TTAGGGAGGG AAGAAGAGCT TCTTCCGTAG TGTGAAGGTG GGCTGTGGCA TCTGCTTTCC
  421 CTTGTTCCTC CTGTCGTCTT TATTGCTCAC GTCATAGTTC ACGCCAGTCA GGATGCTAAG
  481 AATGTCATCT CCTTGAGGAC ATCTTTCCCT CAGGCTCTGG CAAAGTGACT GAATATACCA
  541 GGTTCCATTC ACAGGATCTC GGTAGGAAAC GCAGTTCTTC ACCGTAGCCA TTCCCAGCAG
  601 AAAGTCTGCC TCATCCGGAA TATAGTTCTT GTGAGATGAT GAATCCACTT CTAAAGTGTG
  661 GTTCTGTTGC TCGAAGCCTG CCTCATCAGG CACTCCTTTC TGGAAGTTAC TTCCTTGGCA
  721 AGCCTGAATG AAAAAGATCT TGGGTTTCCC AGACAGGGAA GGGCACTTTG AACCAGTGAA
  781 GTAAGATGTC AGGTCATAGA TGGAGGCCTC CTTCCCATCC GTTCCATAGA CGACACCCTT
  841 GTCACCGTGG GATAGGATAC AGCAGATGAA GCAGTCTTTG TTCTTGTGGT CTGCGCTTTG
  901 GTAGCCTTCT AGAATCTCGT GGGATTTCAT TTGCAGTGCA GTCGTCGTAA GATACTATCT
  961 CAAAATGAAG CTCCTTTAAA GGTCTTACTC AGAGCCTCTT TATCACAGTC TGGTCCCTTT
 1021 TTCTTGTCCC ATCCATTTTT CGAGTGGGAT AATGTCATTC CTGGTCTTGG CTGGAAATTC
1081 AATGCAATTG ATTTGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sakamaki, K., Molecular cloning and characterization of mouse caspase-8. Eur. J. Biochem., 253, 399-405 (1998). PMID 9654089.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl