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pmmCasp8 (Mouse caspase-8) (#RDB08071)

Plasmid clone of mouse caspase-8 cDNA

Alternative name pBS-MmCasp8
Clone info. Plasmid clone of mouse caspase-8 cDNA.
Vector backbone pBluescript SK (Plasmid)
Size of vector backbone 3,204 bp
Selectable markers Amp^r
Gene/insert name Mouse caspase 8 cDNA
Reference of insert sequence AF067834 (DDBJ accession number)
Depositor|Developer Sakamaki, Kazuhiro |
 

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08071 pmmCasp8 (Mouse caspase-8) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmmCasp8 (Mouse caspase-8) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08071).

Reference section:

Sakamaki, K., Tsukumo, S., Yonehara, S., Molecular cloning and characterization of mouse caspase-8. Eur. J. Biochem., 253, 399-405 (1998). PMID 9654089. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18370_B0Glp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: T7 pro,insert 5'
Sequence file: RDB18370_B0Gla.seq check
>08071_18370_B0Gl_5_M13_-40_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGCGAATT GGGTACCGGG CCCCCCCTCG
   61 AGGTCGACGG TATCGATAAG CTTGATATCG AATTCGGCAC GAGGCTAAAC ATTCGGAGGC
  121 ATTTCTGTCC CCTATGCCCT AGTTCTCTCA GTTGTCTTTC ATTCTGACTT CGGTGCTTAA
  181 AAGTCCAGCT TCTCGGAATC GGTAGCAAAC CTCTGTGAGC CGGCGTGGAA CAGGAAGTGA
  241 GTACAGTTCT GGGGAGCGCC GGCCCGGGCT GGAGGCTCGG AAAGCCCAAG CCAGCGGAGG
  301 CCTCGCCCGA GCTGGAGTTG TGACCGGCGC GGCAGGTACT CGGCCACAGG TTACAGCTCT
  361 TCTACCTCTT GATAAGAATG GATTTCCAGA GTTGTCTTTA TGCTATTGCT GAAGAACTGG
  421 GCAGTGAAGA CCTGGCTGCC CTCAAGTTCC TGTGCTTGGA CTACATCCCA CACAAGAAGC
  481 AGGAGACCAT CGAGGATGCC CAGAAGCTAT TTCTGAGGCT GCGGGAAAAG GGGATGTTGG
  541 AGGAAGGCAA TCTGTCTTTC CTGAAAGAGC TGCTTTTCCA CATCAGTCGG TGGGACCTGC
  601 TGGTCAACTT CCTAGACTGC AACCGAGAGG AGATGGTGAG AGAGCTGCGG GATCCAGACA
  661 ATGCCCAGAT TTCTCCCTAC AGGGTCATGC TCTTTAAGCT CTCAGAAGAA GTGAGCGAGT
  721 TGGAATTGAG ATCTTTTAAG TTCCTTTTGA ACAATGAGAT CNNCAAATGT AAGCTGGAAG
  781 ANGANTTGAG CCTGCTTGAA ATTTTTGNAG AAATGGAGAA NANGANCATG CTGGCAGAAA
  841 ATNACTTGGA AANCCTAAAA TCNATCTGTG ANCNGGNCAA CNAGANNCNG NTGGGGAANA
  901 TNNAGGNNTA NGAAANATCN ANCACAGANA NAANAATGNN NNNTGNANGN AANGGNANAN
961 NNGNNNCNNT CANTTTTGGA NGANANGANN NNNAANNNNN NNNNN
//
Primer: mCasp8-B_F (Pr0888)
Region: insert 3',T3 pro,lacO,lac pro,CAP binding site
Sequence file: RDB18370_B0Glb.seq check
>08071_18370_BoGl_5_mCasp8-B_F_D05_04_ABI08.ab1
    1 NNNNNNNNNN NANNATNNTC CGGNNGAGGC AGANTTTCTG CTGGGAATGG CTACGGTGAA
   61 GAACTGCGTT TCCTACCGAG ATCCTGTGAA TGGAACCTGG TATATTCAGT CACTTTGCCA
  121 GAGCCTGAGG GAAAGATGTC CTCAAGGAGA TGACATTCTT AGCATCCTGA CTGGCGTGAA
  181 CTATGACGTG AGCAATAAAG ACGACAGGAG GAACAAGGGA AAGCAGATGC CACAGCCCAC
  241 CTTCACACTA CGGAAGAAGC TCTTCTTCCC TCCCTAATGA TGTGTGCTCT CCACAGTTCA
  301 CATGGCTTAT CTGTGCACTT TTGTGTGGAT GAGTCTAATT TATTTTTTAG AATTTCTTTT
  361 GCTTTTGAAT TTACATTTAC ATAATTTTCC CTTTTCTTCC CTTTAAACCC TTCTTTGTTA
  421 TGTTCCAATT TCAAATACAT GGCCTCTTTT CTCATTAACT GTTGTACACA CACATACATA
  481 CACACACACA CACACACACA CACATTTCTA AATATAACCT GTATACTATC ACTTGTAAAA
  541 AAAAAAAAAA AAAAAATTCC TGCGGCCGCC ACCGCGGTGG AGCTCCAGCT TTTGTTCCCT
  601 TTAGTGAGGG TTAATTTCGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA
  661 TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
  721 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA
  781 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CNACGCGCGG GGNNAGGCGG
  841 TTTGCGNATN NNNNGCTCTT CCGCTTCCTC GCTCACTNAC TCGCTGCGCN CGGNCGTTCN
  901 GCTGNGGNGA NCGGNATCAN CTCANTCNAN NGCGGNAATA CGNNTATNCN NAGAATCNNG
961 GNNNNANNNN NGANNNNNNN NGNNNNNAAA NNNNNNNNNA ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sakamaki, K., Molecular cloning and characterization of mouse caspase-8. Eur. J. Biochem., 253, 399-405 (1998). PMID 9654089. [link to RRC of NBRP]