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pCMV5a-AID-c-Myc-His

Expression vector of mouse Aicda tagged with Myc and His

Catalog number RDB08057
Resource name pCMV5a-AID-c-Myc-His
Alternative name #11388
Clone info. Expression vector of mouse Aicda tagged with Myc and His.
Note for vector-insert junction: vector-gaattcgccATG---(AID)---TTTggtacc-Myc.His-TGA---ctc ggc-vector.
Vector backbone pFLAG-CMV-5a (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Aid/Arp2 cDNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nat. Med., 13, 470-4766 (2007)). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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RDB08057 pCMV5a-AID-c-Myc-His DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Matsumoto, Y., Helicobacter pylori infection triggers aberrant expression of activation-induced cytidine deaminase in gastric epithelium. Nat. Med., 13, 470-4766 (2007). PMID 17401375.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08057_A4F2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB08057_A4F2a.seq
>08057_08057_A4F2_CMV-Foward_D10_10.ab1
    1 ATGATATTAC CCCGGCCCCG GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGAATTCGC CATGGACAGC CTTCTGATGA
  121 AGCAAAAGAA GTTTCTTTAC CATTTCAAAA ATGTCCGCTG GGCCAAGGGA CGGCATGAGA
  181 CCTACCTCTG CTACGTGGTG AAGAGGAGAG ATAGTGCCAC CTCCTGCTCA CTGGACTTCG
  241 GCCACCTTCG CAACAAGTCT GGCTGCCACG TGGAATTGTT GTTCCTACGC TACATCTCAG
  301 ACTGGGACCT GGACCCGGGC CGGTGTTACC GCGTCACCTG GTTCACCTCC TGGAGCCCGT
  361 GCTATGACTG TGCCCGGCAC GTGGCTGAGT TTCTGAGATG GAACCCTAAC CTCAGCCTGA
  421 GGATTTTCAC CGCGCGCCTC TACTTCTGTG AAGACCGCAA GGCTGAGCCT GAGGGGCTGC
  481 GGAGACTGCA CCGCGCTGGG GTCCAGATCG GGATCATGAC CTTCAAAGAC TATTTTTACT
  541 GCTGGAATAC ATTTGTAGAA AATCGTGAAA GAACTTTCAA AGCCTGGGAA GGGCTACATG
  601 AAAATTCTGT CCGGCTAACC AGACAACTTC GGCGCATCCT TTTGCCCTTG TACGAAGTCG
  661 ATGACTTGCG AGATGCATTT CGTATGTTGG GATTTGGTAC CAAGCTTGGG CCCGAACAAA
  721 AACTCATCTC AGAAGAGGAT CTGAATAGCG CCGTCGACCA TCATCATCAT CATCATTGAG
  781 TTTAAACGGT CTCCAGCTTA ACCTCGAGGG CCACTGGGTG GCATCCCTGT GACCCCTCCC
  841 CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA
  901 AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGGTGGAGG
  961 GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GGAAAGACAA CCTGTTAGGG CCTGCGGGGT
 1021 TCTATTGGGA ACCCAGGCTG GAGTTGCAGT GGCCACAATC TTGGGCTCAA CTGCAAATCT
 1081 TCCGCCCTTC TGGGATTCAA GCGATTCCTC CCTGGCTCAG CCTCCCGAGT TGTGGGATCC
1141 CAAGCCATGG CATTGAAACC AGGGGCCTCT CA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsumoto, Y., Helicobacter pylori infection triggers aberrant expression of activation-induced cytidine deaminase in gastric epithelium. Nat. Med., 13, 470-4766 (2007). PMID 17401375.

2019.02.05

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