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pCMV5a-AID-c-Myc-His

Expression vector of mouse Aicda tagged with Myc and His

Catalog number RDB08057
Resource name pCMV5a-AID-c-Myc-His
Alternative name #11388
Clone info. Expression vector of mouse Aicda tagged with Myc and His.
Comment Note for vector-insert junction: vector-gaattcgccATG---(AID)---TTTggtacc-Myc.His-TGA---ctc ggc-vector.
Vector backbone pFLAG-CMV-5a (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Aid/Arp2 cDNA
Depositor|Developer Honjo, Tasuku |
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External Database
Mouse Aid/Arp2
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Nat. Med., 13, 470-4766 (2007)). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nat. Med., 13, 470-4766 (2007))。公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
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          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08057 pCMV5a-AID-c-Myc-His DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMV5a-AID-c-Myc-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08057).

          Reference section:

          Matsumoto, Y., Marusawa, H., Kinoshita, K., Endo, Y., Kou, T., Morisawa, T., Azuma, T., Okazaki, I.M., Honjo, T., Chiba, T., Helicobacter pylori infection triggers aberrant expression of activation-induced cytidine deaminase in gastric epithelium. Nat. Med., 13, 470-4766 (2007). PMID 17401375. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08057_A4F2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB08057_A4F2a.seq check
          >08057_08057_A4F2_CMV-Foward_D10_10.ab1
              1 ATGATATTAC CCCGGCCCCG GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGAATTCGC CATGGACAGC CTTCTGATGA
            121 AGCAAAAGAA GTTTCTTTAC CATTTCAAAA ATGTCCGCTG GGCCAAGGGA CGGCATGAGA
            181 CCTACCTCTG CTACGTGGTG AAGAGGAGAG ATAGTGCCAC CTCCTGCTCA CTGGACTTCG
            241 GCCACCTTCG CAACAAGTCT GGCTGCCACG TGGAATTGTT GTTCCTACGC TACATCTCAG
            301 ACTGGGACCT GGACCCGGGC CGGTGTTACC GCGTCACCTG GTTCACCTCC TGGAGCCCGT
            361 GCTATGACTG TGCCCGGCAC GTGGCTGAGT TTCTGAGATG GAACCCTAAC CTCAGCCTGA
            421 GGATTTTCAC CGCGCGCCTC TACTTCTGTG AAGACCGCAA GGCTGAGCCT GAGGGGCTGC
            481 GGAGACTGCA CCGCGCTGGG GTCCAGATCG GGATCATGAC CTTCAAAGAC TATTTTTACT
            541 GCTGGAATAC ATTTGTAGAA AATCGTGAAA GAACTTTCAA AGCCTGGGAA GGGCTACATG
            601 AAAATTCTGT CCGGCTAACC AGACAACTTC GGCGCATCCT TTTGCCCTTG TACGAAGTCG
            661 ATGACTTGCG AGATGCATTT CGTATGTTGG GATTTGGTAC CAAGCTTGGG CCCGAACAAA
            721 AACTCATCTC AGAAGAGGAT CTGAATAGCG CCGTCGACCA TCATCATCAT CATCATTGAG
            781 TTTAAACGGT CTCCAGCTTA ACCTCGAGGG CCACTGGGTG GCATCCCTGT GACCCCTCCC
            841 CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA
            901 AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGGTGGAGG
            961 GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GGAAAGACAA CCTGTTAGGG CCTGCGGGGT
           1021 TCTATTGGGA ACCCAGGCTG GAGTTGCAGT GGCCACAATC TTGGGCTCAA CTGCAAATCT
           1081 TCCGCCCTTC TGGGATTCAA GCGATTCCTC CCTGGCTCAG CCTCCCGAGT TGTGGGATCC
          1141 CAAGCCATGG CATTGAAACC AGGGGCCTCT CA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Matsumoto, Y., Helicobacter pylori infection triggers aberrant expression of activation-induced cytidine deaminase in gastric epithelium. Nat. Med., 13, 470-4766 (2007). PMID 17401375. [link to RRC of NBRP]

          2023.10.05

          GNP_filter3_RDBDEP_html_231001.pl