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pUAST-GFPN-lacZ

P-element mediated gene transfer vector of GFP-N and lacZ.

Catalog number RDB08035
Resource name pUAST-GFPN-lacZ
Clone info. P-element mediated gene transfer vector of GFP-N and lacZ.
Vector backbone pUAST (Plasmid)
Size of vector backbone 9.0 kb
Selectable markers Amp^r
Gene/insert name E. coli lacZ genomic DNA Aequorea victoria GFP cDNA
Depositor|Developer Hayashi, Shigeo |
Other clones in our bank

External Database
E. coli lacZ Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Develop. Growth Differ., 38, 99-106 (1996)).
          Remarks
          Please use Schedule A (GFP-A) additionally.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Develop. Growth Differ., 38, 99-106 (1996))。
          備考
          Schedule A(GFP-A, 英語版のみ)が追加で必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08035 pUAST-GFPN-lacZ DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Shiga, Y., A nuclear GFP/beta-galactosidase fusion protein as a marker for morphogenesis in living Drosophila. Develop. Growth Differ., 38, 99-106 (1996).

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0056e (English text)
          Featured content Dnaconda's recommendation EXR0056j (Japanese text)
          Featured content Empty Backbone (English text)
          Featured content Empty Backbone (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08035_A5Kap1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUAST3'
          Sequence file: RDB08035_A5Kaa.seq check
          >08035_08035_A5Ka_pUAST3'_B10_04.ab1
              1 CTGCCAGTAG TATTATTGAA TACAAGAAGA GAACTCTGAA TAGGGAATTG GGAATTCGTT
             61 AACAGATCTG CGGCCGCGGC TCGAGGGTAC CGAGCTCAGA AAAAATGACT GCTCCAAAGA
            121 AGAAGCGTAA GGGCGCCCCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG
            181 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG
            241 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG
            301 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTTT CTCTTATGGT GTTCAATGCT
            361 TTTCAAGATA CCCAGATCAT ATGAAACGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG
            421 GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTGCTG
            481 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA
            541 AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT
            601 ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTAA CTTCAAAATT AGACACAACA
            661 TTGAAGATGG AAGCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG
            721 GCCCTGTCCT TTTACCAGAC AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC
            781 CCAACGAAAA GAGAGACCAC ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC
            841 ATGGCATGGA TGAACTATAC AAAGCTTCGG ATCCCGTCGT TTTACAACGT CGTGACTGGG
            901 AAAACCCTGG CGTTACCCAA CTTTAATCGC CTTGCAGCAC ATCCCCCCTT TCGCCAGCTG
            961 GCGTAAATAG CGAAGAGCCG CACGATCGCC CTTCCCACAG TGCGCAGCCT GAATGGCCGA
          1021 TGGACGCTTT GGCTGATCCA GGCATCAGCA GCCGGATGCC GGAAAGGCCT G
          //
          Primer: SV40-3'UTR
          Sequence file: RDB08035_A5Kab.seq check
          >08035_08035_A5Ka_SV40-3'UTR_C10_07.ab1
              1 ATCCAAAAAG TAGGTTCCTT CACAAAGATC CTCTAGAGGT ACCCAAATAG TACATAATGG
             61 ATTTCCTTAC GCGAAATACG GGCAGACATG GCCTGCCCGG TTATTATTAT TTTTGACACC
            121 AGACCAACTG GTAATGGTAG CGACCGGCGC TCAGCTGTAA TTCCGCCGAT ACTGACGGGC
            181 TCCAGGAGTC GTCGCCACCA ATCCCCATAT GGAAACCGTC GATATTCAGC CATGTGCCTT
            241 CTTCCGCGTG CAGCAGATGG CGATGGCTGG TTTCCATCAG TTGCTGTTGA CTGTAGCGGC
            301 TGATGTTGAA CTGGAAGTCG CCGCGCCACT GGTGTGGGCC ATAATTCAAT TCGCGCGTCC
            361 CGCAGCGCAG ACCGTTTTCG CTCGGGAAGA CGTACGGGGT ATACATGTCT GACAATGGCA
            421 GATCCCAGCG GTCAAAACAG GCGGCAGTAA GGCGGTCGGG ATAGTTTTCT TGCGGCCCTA
            481 ATCCGAGCCA GTTTACCCGC TCTGCTACCT GCGCCAGCTG GCAGTTCAGG CCAATCCGCG
            541 CCGGATGCGG TGTATCGCTC GCCACTTCAA CATCAACGGT AATCGCCATT TGACCACTAC
            601 CATCAATCCG GTAGGTTTTC CGGCTGATAA ATAAGGTTTT CCCCTGATGC TGCCACGCGT
            661 GAGCGGTCGT AATCAGCACC GCATCAGCAA GTGTATCTGC CGTGCACTGC AACAACGCTG
            721 CTTCGGCCTG GTAATGGCCC GCCGCCTTCC AGCGTTCGAC CCAGGCGTTA GGGTCAATGC
            781 GGGTCGCTTC ACTTACGCCA ATGTCGTTAT CCAGCGGTGC ACGGGTGAAA CTGATCGCGC
            841 AGCGGCGTCA GCAGTTGTTT TTTTATCGCC CAATCCACAT CTGTGAAAAG AAAGCCTGAC
            901 TGGCGGTTAA ATTGCCCAAC GCTTATTTAC CCAGCTCGAT GCAAAAAATC CAATTTTCGC
            961 TGGGTGGGTC AGATGCGGGA TGGCGTTGAG ACGCGGGCGG GGAGCGTCAC AACTGAGGTT
           1021 TTTCCGCCAG ACGGCCACTG CTGGCCAGGC GCTGATGTAC CGGCCTTCTG GACCATGGCC
          1081 GGGATCCGCC GGATTTC
          //
          Primer: GFP-C
          Sequence file: RDB08035_A5Kac.seq check
          >08035_08035_A5Ka_GFP-C_C06_09.ab1
              1 CTCACCATTA CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA
             61 CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG ATGAACTATA
            121 CAAAGCTTCG GATCCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA
            181 ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG
            241 CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCT TTGCCTGGTT
            301 TCCGGCACCA GAAGCGGTGC CGGAAAGCTG GCTGGAGTGC GATCTTCCTG AGGCCGATAC
            361 TGTCGTCGTC CCCTCAAACT GGCAGATGCA CGGTTACGAT GCGCCCATCT ACACCAACGT
            421 GACCTATCCC ATTACGGTCA ATCCGCCGTT TGTTCCCACG GAGAATCCGA CGGGTTGTTA
            481 CTCGCTCACA TTTAATGTTG ATGAAAGCTG GCTACAGGAA GGCCAGACGC GAATTATTTT
            541 TGATGGCGTT AACTCGGCGT TTCATCTGTG GTGCAACGGG CGCTGGGTCG GTTACGGCCA
            601 GGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT TTACGCGCCG GAGAAAACCG
            661 CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT CTGGAAGATC AGGATATGTG
            721 GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT AAACCGACTA CACAAATCAG
            781 CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCAGC CGCGCTGTAC TGGAGGCTGA
            841 AGTTCAGATG TGCGGCGAGT TGCGTGACTA CCTACGGGTA ACAGTTTCTT TATGGCAGGG
            901 TGAAACGCAG TCGCCAGCGG CACCGCGCCT TTCGGCGGTG AAATTATCGA TGAGCGTGGT
            961 GGTTATGCCG ATCGCGTCAC ACTACGTCTG ACGTCGAAAA CCCGAAACTG TGAGCGCCGA
           1021 AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACG GCGGACCGCA CGGCTGATTG
           1081 AAGCCGAGCC TGCGATGTCC GGACTTCGCG AAGGTGCGGA ATTGGAAAAA TGGATCCTGC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shiga, Y., A nuclear GFP/beta-galactosidase fusion protein as a marker for morphogenesis in living Drosophila. Develop. Growth Differ., 38, 99-106 (1996).

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl