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pUAST-GFPN-lacZ

P-element mediated gene transfer vector of GFP-N and lacZ.

Catalog number RDB08035
Resource name pUAST-GFPN-lacZ
Clone info. P-element mediated gene transfer vector of GFP-N and lacZ.
Vector backbone pUAST (Plasmid)
Size of vector backbone 9.0 kb
Selectable markers Amp^r
Gene/insert name E. coli lacZ genomic DNA Aequorea victoria GFP cDNA
Depositor Hayashi, Shigeo |
 
Other clones in our bank E. coli lacZ (KEGG orthology K01190) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Develop. Growth Differ., 38, 99-106 (1996)).
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08035 pUAST-GFPN-lacZ DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Shiga, Y., A nuclear GFP/beta-galactosidase fusion protein as a marker for morphogenesis in living Drosophila. Develop. Growth Differ., 38, 99-106 (1996).

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08035_A5Kap1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUAST3' RDB08035_A5Kaa.seq
>08035_08035_A5Ka_pUAST3'_B10_04.ab1
    1 CTGCCAGTAG TATTATTGAA TACAAGAAGA GAACTCTGAA TAGGGAATTG GGAATTCGTT
   61 AACAGATCTG CGGCCGCGGC TCGAGGGTAC CGAGCTCAGA AAAAATGACT GCTCCAAAGA
  121 AGAAGCGTAA GGGCGCCCCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG
  181 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG
  241 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG
  301 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTTT CTCTTATGGT GTTCAATGCT
  361 TTTCAAGATA CCCAGATCAT ATGAAACGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG
  421 GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTGCTG
  481 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA
  541 AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT
  601 ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTAA CTTCAAAATT AGACACAACA
  661 TTGAAGATGG AAGCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG
  721 GCCCTGTCCT TTTACCAGAC AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC
  781 CCAACGAAAA GAGAGACCAC ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC
  841 ATGGCATGGA TGAACTATAC AAAGCTTCGG ATCCCGTCGT TTTACAACGT CGTGACTGGG
  901 AAAACCCTGG CGTTACCCAA CTTTAATCGC CTTGCAGCAC ATCCCCCCTT TCGCCAGCTG
  961 GCGTAAATAG CGAAGAGCCG CACGATCGCC CTTCCCACAG TGCGCAGCCT GAATGGCCGA
1021 TGGACGCTTT GGCTGATCCA GGCATCAGCA GCCGGATGCC GGAAAGGCCT G
//
Primer: SV40-3'UTR RDB08035_A5Kab.seq
>08035_08035_A5Ka_SV40-3'UTR_C10_07.ab1
    1 ATCCAAAAAG TAGGTTCCTT CACAAAGATC CTCTAGAGGT ACCCAAATAG TACATAATGG
   61 ATTTCCTTAC GCGAAATACG GGCAGACATG GCCTGCCCGG TTATTATTAT TTTTGACACC
  121 AGACCAACTG GTAATGGTAG CGACCGGCGC TCAGCTGTAA TTCCGCCGAT ACTGACGGGC
  181 TCCAGGAGTC GTCGCCACCA ATCCCCATAT GGAAACCGTC GATATTCAGC CATGTGCCTT
  241 CTTCCGCGTG CAGCAGATGG CGATGGCTGG TTTCCATCAG TTGCTGTTGA CTGTAGCGGC
  301 TGATGTTGAA CTGGAAGTCG CCGCGCCACT GGTGTGGGCC ATAATTCAAT TCGCGCGTCC
  361 CGCAGCGCAG ACCGTTTTCG CTCGGGAAGA CGTACGGGGT ATACATGTCT GACAATGGCA
  421 GATCCCAGCG GTCAAAACAG GCGGCAGTAA GGCGGTCGGG ATAGTTTTCT TGCGGCCCTA
  481 ATCCGAGCCA GTTTACCCGC TCTGCTACCT GCGCCAGCTG GCAGTTCAGG CCAATCCGCG
  541 CCGGATGCGG TGTATCGCTC GCCACTTCAA CATCAACGGT AATCGCCATT TGACCACTAC
  601 CATCAATCCG GTAGGTTTTC CGGCTGATAA ATAAGGTTTT CCCCTGATGC TGCCACGCGT
  661 GAGCGGTCGT AATCAGCACC GCATCAGCAA GTGTATCTGC CGTGCACTGC AACAACGCTG
  721 CTTCGGCCTG GTAATGGCCC GCCGCCTTCC AGCGTTCGAC CCAGGCGTTA GGGTCAATGC
  781 GGGTCGCTTC ACTTACGCCA ATGTCGTTAT CCAGCGGTGC ACGGGTGAAA CTGATCGCGC
  841 AGCGGCGTCA GCAGTTGTTT TTTTATCGCC CAATCCACAT CTGTGAAAAG AAAGCCTGAC
  901 TGGCGGTTAA ATTGCCCAAC GCTTATTTAC CCAGCTCGAT GCAAAAAATC CAATTTTCGC
  961 TGGGTGGGTC AGATGCGGGA TGGCGTTGAG ACGCGGGCGG GGAGCGTCAC AACTGAGGTT
 1021 TTTCCGCCAG ACGGCCACTG CTGGCCAGGC GCTGATGTAC CGGCCTTCTG GACCATGGCC
1081 GGGATCCGCC GGATTTC
//
Primer: GFP-C RDB08035_A5Kac.seq
>08035_08035_A5Ka_GFP-C_C06_09.ab1
    1 CTCACCATTA CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA
   61 CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG ATGAACTATA
  121 CAAAGCTTCG GATCCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA
  181 ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG
  241 CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCT TTGCCTGGTT
  301 TCCGGCACCA GAAGCGGTGC CGGAAAGCTG GCTGGAGTGC GATCTTCCTG AGGCCGATAC
  361 TGTCGTCGTC CCCTCAAACT GGCAGATGCA CGGTTACGAT GCGCCCATCT ACACCAACGT
  421 GACCTATCCC ATTACGGTCA ATCCGCCGTT TGTTCCCACG GAGAATCCGA CGGGTTGTTA
  481 CTCGCTCACA TTTAATGTTG ATGAAAGCTG GCTACAGGAA GGCCAGACGC GAATTATTTT
  541 TGATGGCGTT AACTCGGCGT TTCATCTGTG GTGCAACGGG CGCTGGGTCG GTTACGGCCA
  601 GGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT TTACGCGCCG GAGAAAACCG
  661 CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT CTGGAAGATC AGGATATGTG
  721 GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT AAACCGACTA CACAAATCAG
  781 CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCAGC CGCGCTGTAC TGGAGGCTGA
  841 AGTTCAGATG TGCGGCGAGT TGCGTGACTA CCTACGGGTA ACAGTTTCTT TATGGCAGGG
  901 TGAAACGCAG TCGCCAGCGG CACCGCGCCT TTCGGCGGTG AAATTATCGA TGAGCGTGGT
  961 GGTTATGCCG ATCGCGTCAC ACTACGTCTG ACGTCGAAAA CCCGAAACTG TGAGCGCCGA
 1021 AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACG GCGGACCGCA CGGCTGATTG
 1081 AAGCCGAGCC TGCGATGTCC GGACTTCGCG AAGGTGCGGA ATTGGAAAAA TGGATCCTGC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Shiga, Y., A nuclear GFP/beta-galactosidase fusion protein as a marker for morphogenesis in living Drosophila. Develop. Growth Differ., 38, 99-106 (1996).

2018.12.10

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