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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phPOU5F1

Promoter collection, Human POU5F1 promoter

Catalog number RDB07912
Resource name pGL4-phPOU5F1
Alternative name U3040-6
Clone info. PCR amplified human POU5F1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 31172142 to 31170650(NC_000006.12); relatively -1460 to +33, where +1 corresponds to 1 nt of NM_002701.6.
Comment PCR cloning, forward primer: ATTCTGTGTGAGGGGATTGG; reverse primer: CTGGTGAAATGAGGGCTTG. Known differences (at least): substitutions with NC_000006.12, G to A at 31,171,784nt, C to T at 31,171,713nt, T to C at 31,171,675nt, G to A at 31,170,930nt and C to T at 31,170,695nt. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human POU class 5 homeobox 1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human POU class 5 homeobox 1

          Reference sequence
            

          Distribution information

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          RDB07912 pGL4-phPOU5F1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07912_A1Ebp1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F
          Region: pause site,insert5'
          Sequence file: RDB07912_A1Eba.seq check
          >07912_07912_A9IdF_pGL4ins_F_B05_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNCN NGNNNNNNNA GGCTGTCCCC AGTGCAAGTG CAGGTGCCAG
             61 AACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG ATATTCTGTG
            121 TGAGGGGATT GGGACTGGGG GGTTGGGGAG CAGGAAGCAG TCCCCAGGGG AGCCATCCAG
            181 GCCCATTCAA GGGTTGAGCA CTTGTTTAGG GTTAGAGCTG CCCCCTCTGG GGACCGGGAT
            241 TGTCCAGCCA AGGCCATTGT CCTGCCCCCT TCCCCCAGTC CCTCCCAGGC TTCTTTGAAC
            301 CTGAAGTCAG ATATTTTTTC TCCACACCCC CCACCCCCTG GTTTTCCCCA CCCAGGGCCT
            361 AGGGCTGGAG GCCTGGGCCA GGGAGGTGGG GGAGGGAGAA CGGGGCCTAC CGTGGTATTA
            421 GATGTCTGAG TTTTGGTTGA GAGGGGAGCA AGGAACCTGA TGTGCAGGTT TCATAGTGGA
            481 GGGGGCCCAA AGCGGGTGTC TTATCACTCT GTTTCAGCAA AGGTTGGGAA ACTGAGGCCC
            541 AATCAGTCCA AAGTCTGGTC CCTTGAAGGG GAAGTAGGGG CCAACCCCTT AGTCTGTTAG
            601 ATGAGGAGAG TCTGGAGTCT GATTCTGGAA GACGGAGGGG TGGGGGGATG GGGGGTGGNN
          //
          Primer: pGL4ins_R
          Region: Luciferase5',insert3'
          Sequence file: RDB07912_A1Ebb.seq check
          >07912_07912_A9IdF_pGL4ins_R_H02_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GCTGGGCCCT TCTTAATGTT TTTGGCNTCT
             61 TCCNTGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT GGCCGCCGAG GCCAGATCTT
            121 GATCTGGTGA AATGAGGGCT TGCGAAGGGA CTACTCAACC CCTCTCTCTC TCCCCAGTCC
            181 CACCCACTAG CCTTGACCTC TGGCCCCGCC CCCTGGATGG GTGGAGGAGA GGGAGGTGGG
          241 GGNNN
          //
          Primer: pGL4pA_R
          Region: SV40p(A),Luciferase3'
          Sequence file: RDB07912_A1Ebc.seq check
          >07912_07912_A9IdF_pGL4pA_R_D05_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTNTCGNNTT NNCNNCNTTT GTAGAGGTTT TACTTGCTTT
             61 AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT
            121 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
            181 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
            241 TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC ACGGCGATCT
            301 TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC AACTTGCCGG
            361 TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC TTGGCGGTTG
            421 TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA CCGTGTTCCA
            481 GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG GCGACCCCGG
            541 CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG GCTACCTGGT
            601 AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC TCGTCCTCGT
            661 CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA GCGTTTGTAG
            721 CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC AGCTCGCCGC
            781 GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTANCC TCNAANANNG
            841 GCACCACCTT GCCNACTGCG CCAGGCTNGT CGNCCCCTTC NGGGGGNNAT CANAANNGGC
            901 GCNNGNTGTT TCTGNCNNNN CGTANNCCCT NGNNGNATGC CNNNNNNGTG GNANNCGTTT
          961 NGNCNNCNNN CNNCANCNTA NNNNCNTNNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl