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pGL4-phPOU5F1 (#RDB07912)

Promoter collection, Human POU5F1 promoter

Alternative name U3040-6
Clone info. PCR amplified human POU5F1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 31172142 to 31170650(NC_000006.12); relatively -1460 to +33, where +1 corresponds to 1 nt of NM_002701.6.
Comment PCR cloning, forward primer: ATTCTGTGTGAGGGGATTGG; reverse primer: CTGGTGAAATGAGGGCTTG. Known differences (at least): substitutions with NC_000006.12, G to A at 31,171,784nt, C to T at 31,171,713nt, T to C at 31,171,675nt, G to A at 31,170,930nt and C to T at 31,170,695nt. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human POU class 5 homeobox 1 genomic DNA
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07912 pGL4-phPOU5F1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phPOU5F1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07912).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07912_A1Ebp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F ()
Region: pause site,insert5'
Sequence file: RDB07912_A1Eba.seq check
>07912_07912_A9IdF_pGL4ins_F_B05_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNCN NGNNNNNNNA GGCTGTCCCC AGTGCAAGTG CAGGTGCCAG
   61 AACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG ATATTCTGTG
  121 TGAGGGGATT GGGACTGGGG GGTTGGGGAG CAGGAAGCAG TCCCCAGGGG AGCCATCCAG
  181 GCCCATTCAA GGGTTGAGCA CTTGTTTAGG GTTAGAGCTG CCCCCTCTGG GGACCGGGAT
  241 TGTCCAGCCA AGGCCATTGT CCTGCCCCCT TCCCCCAGTC CCTCCCAGGC TTCTTTGAAC
  301 CTGAAGTCAG ATATTTTTTC TCCACACCCC CCACCCCCTG GTTTTCCCCA CCCAGGGCCT
  361 AGGGCTGGAG GCCTGGGCCA GGGAGGTGGG GGAGGGAGAA CGGGGCCTAC CGTGGTATTA
  421 GATGTCTGAG TTTTGGTTGA GAGGGGAGCA AGGAACCTGA TGTGCAGGTT TCATAGTGGA
  481 GGGGGCCCAA AGCGGGTGTC TTATCACTCT GTTTCAGCAA AGGTTGGGAA ACTGAGGCCC
  541 AATCAGTCCA AAGTCTGGTC CCTTGAAGGG GAAGTAGGGG CCAACCCCTT AGTCTGTTAG
  601 ATGAGGAGAG TCTGGAGTCT GATTCTGGAA GACGGAGGGG TGGGGGGATG GGGGGTGGNN
//
Primer: pGL4ins_R ()
Region: Luciferase5',insert3'
Sequence file: RDB07912_A1Ebb.seq check
>07912_07912_A9IdF_pGL4ins_R_H02_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GCTGGGCCCT TCTTAATGTT TTTGGCNTCT
   61 TCCNTGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT GGCCGCCGAG GCCAGATCTT
  121 GATCTGGTGA AATGAGGGCT TGCGAAGGGA CTACTCAACC CCTCTCTCTC TCCCCAGTCC
  181 CACCCACTAG CCTTGACCTC TGGCCCCGCC CCCTGGATGG GTGGAGGAGA GGGAGGTGGG
241 GGNNN
//
Primer: pGL4pA_R ()
Region: SV40p(A),Luciferase3'
Sequence file: RDB07912_A1Ebc.seq check
>07912_07912_A9IdF_pGL4pA_R_D05_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNTCGNNTT NNCNNCNTTT GTAGAGGTTT TACTTGCTTT
   61 AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT
  121 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
  181 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
  241 TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC ACGGCGATCT
  301 TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC AACTTGCCGG
  361 TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC TTGGCGGTTG
  421 TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA CCGTGTTCCA
  481 GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG GCGACCCCGG
  541 CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG GCTACCTGGT
  601 AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC TCGTCCTCGT
  661 CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA GCGTTTGTAG
  721 CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC AGCTCGCCGC
  781 GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTANCC TCNAANANNG
  841 GCACCACCTT GCCNACTGCG CCAGGCTNGT CGNCCCCTTC NGGGGGNNAT CANAANNGGC
  901 GCNNGNTGTT TCTGNCNNNN CGTANNCCCT NGNNGNATGC CNNNNNNGTG GNANNCGTTT
961 NGNCNNCNNN CNNCANCNTA NNNNCNTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles