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pGL3-AID R1+R4 full

Reporter construct of mouse Aicda locus, position -9226 to -7585 plus -1527 to +101.

Catalog number RDB07880
Resource name pGL3-AID R1+R4 full
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -7585 plus -1527 to +101.
Nat. Immunol. (2010) 2, 148-154, Fig. 1b.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07880 pGL3-AID R1+R4 full DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07880_A3Af.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3_RV3 RDB07880_A3Afa.seq
>7880_7880_A3Af_pGL3-Rv3_E02_14.ab1
    1 GGTTAGGGGC AGACATTTCT CTATCGATAG GTACCCACCA CTCTCGGCTG TTCTGGAACT
   61 CTCTGTAACC TCGGGCTGTA TGAGTGTATG TGAGTGTGTG TATATGGGTG TGGGGGGGGT
  121 GAGAGTATAT GAGTGTATGT GAGTGTGTAT GTATGGGTGT GAGGTGTGTG TGTGTATGAG
  181 TGTGTGTATG AGTGTGTGTA TGAGTGTGTG TGAGTGTGTG TATATGGGGG GGTGACCCCC
  241 GATGCTTTCC AGAGACCGCC AGCCCCTCAT GCTGGCCATA ACGAAACCTC ACCTGTGTGT
  301 CTCATAGGGA AAGCGAGGTG GTGAACTCCA GATGTCCAAA ACTCAGCTCA GCCGCAGGCG
  361 ACTCGATTTC CAAGTCACAG TATTTTTGGT TAAAAACAAT GTGACCAAGT TGGCTTGTGG
  421 TTTTTCAATA GAAGTTAATG AGGGGAAAGA GAAGTTAATG AAGAAAATAA AAGTTGATCA
  481 AAACCCTGTA AACTGCTTTG CCGCTGTACC AGTTCCAAAT TTCCCGGGAC TGGAAAATGG
  541 TTTTTGTAAC TAAGTGAGAT CTCTTTCCTT CCTTTAGAAG AAGGCAAATG ATAGAATTTG
  601 GCTTTCTTAC AAATCAAGGG TTTTGTATTT GTTCCTATGG CATGTGTACG GCATCTGTAA
  661 GAGAGGGTGT GGGCGTGCGT GTGCGTGTGC TACAGCTCGA GGTGTCTCCA GTATGTCATT
  721 CCCTGGACTA TTGGTCCAAA GTAGGGCAAA GGTCCGAGCC CTCATCCTGT GAGAACAAGT
  781 TAAAAGGGAC TTGTCCCACC CCACCACCCT GGGGGTTCCC CTTAACCCTG TAATGTCCAC
  841 GGGCTGGGTG CTGGGTTTCT TCCCGGTGCC CTGAAATTTT TTATTCCAAG AATTCATTAA
901 TTCACTTA
//
Primer: pGL3_RV6R RDB07880_A3Afb.seq
>7880_7880_A3Af_pGL3-Rv6R_F02_17.ab1
    1 GTATCCGTCA GCGGATAGAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG
   61 ATCGGTCTCC AGCGTGACTT TCTTGACTCC CTCAAAGTCT TAGGCCAAAG GAAGCTTGCT
  121 TCAAGCAAGG CTGCTTCAGT GCTGTTGTGT GACAGAGCAC TGGCCCCTGG CATTCAAATC
  181 TGAGCTTGCT CTTGTGGGCT CCTCCCCCAC GACAGCATCA CTACCACCAG GTATCCAACA
  241 TCTGCCTCCT GGGTTGGGTC CAGTAAAGAT GGGCGGGGCT GTGGTGGGCA AAGCCACACG
  301 GATTAAATGA TTGGGGGGGG GGGCCAGTTC TGCTCATGCT TAATGTCCAA CTTCCTTCGA
  361 GGCTGTTCCT CTAAAAAGCC AGCCCAGGTA TCACAGGTAA CAAAGATCAG GAGACAATAG
  421 CACTGGACAC TTCTCACATT GGTATCATGA TAATGAGCTC TGTGAGTGTG TGTGTGTGTG
  481 TGTGTGTGTG GTCCCGGGTA TCACACCTAA GGTCTGTGCA TACTTCCTAA TTTTAAAACT
  541 ATGATAACAA GAATGATAGC AGTCATAGTG ACTTAAAGTG GCCTTAAATG GCACTTCAGT
  601 TGCATCTTGT GCTTCTTGAA CTTACAGTAT CTGTTCCTTC CGTTTGTTAC ACTCAGTTTT
  661 ATGCCTCAGT AGAGATGGGG GTTGACCACC ACAGCATCCA GAGAGTGAAC TTTAGCCTCC
  721 CAATTCTCTA ATATATTTTT CTTTTTTTTA AAATGACATT ATAAGATAGC TAGAAAGACT
  781 AGTTGCTTGC ACCAAAAAGT GATTTATTTT AATTTTGTGT GTGTGTGTGT GTCAAAAGTA
  841 TTAAAAAAAA AAAAGAACTC CTTTTCCTCA TTAAAAATAC CTTACTGGGA GGCCTGGAGA
  901 GCTGGCCCAG TGATTTAGAG CACTTTACTT CTTATTGGCA GAGACAGCAG CAGTGAAGGC
  961 ACAATGTGTG CTGCTGGATC TGACCTTGAT CATCTGAGAA CATCGTTGGC TTCCTTCCTT
1021 CTCGTCGTCG TACTACTACT ACTCATACAT ACATCTCTTA CCCGCAGTGC TA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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