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pGL3-AID R4 mut. NF-kB-2

Reporter construct of mouse Aicda locus

Catalog number RDB07877
Resource name pGL3-AID R4 mut. NF-kB-2
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -7585.
Nat. Immunol. (2010) 2, 148-154, Fig. 4c.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07877 pGL3-AID R4 mut. NF-kB-2 DNA solution

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References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07877_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07877_A4Gia.seq
>07877_07877_A4Gi_pGL4-4174F_A05_02.ab1
    1 GTTCGGGTCC AGGACATTTC TCTATCGATA GGTACCCACC ACTCTCGGCT GTTCTGGAAC
   61 TCTCTGTAAC CTCGGGCTGT ATGAGTGTAT GTGAGTGTGT GTATATGGGT GTGGGGGGGG
//
Primer: Luc_R RDB07877_A4Gib.seq
>07877_07877_A4Gi_Luc_R_B05_05.ab1
    1 CTTTGGCTTC CAGGAACAGG GCGTATCTCT TCATAGCCTT ATGCAGTTGC TCTCCAGCGG
   61 TTCCATCTTC CAGCGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCCA
  121 TGGATCGGTC TCCAGCGTGA CTTTCTTGAC TCCCTCAAAG TCTTAGGCCA AAGGAAGCTT
  181 GCTTCAAGCA AGGCTGCTTC AGTGCTGTTG TGTGACAGAG CACTGGCCCC TGGCATTCAA
  241 ATCTGAGCTT GCTCTTGTGG GCTCCTCCCC CACGACAGCA TCACTACCAC CAGGTATCCA
  301 ACATCTGCCT CCTGGGTTGG GTCCAGTAAA GATGGGCGGG GCTGTGGTGG GCAAAGCCAC
  361 ACGGATTAAA TGATTGGGGG GGGGGGCCAG TTCTGCTCAT GCTTAATGTC CAACTTCCTT
  421 CGAGGCTGTT CCTCTAAACT CGAGCTGTAG CACACGCACA CGCACGCCCA CACCCTCTCT
  481 TACAGATGCC GTACACATGC CATAGGAACA AATACAAAAC CCTTGATTTG TAAGAAAGCC
  541 AAATTCTCTC ATTTGCCTTC TTCTAAAGGA AGGAAAGAGA TCTCACTTAG TTACAAAAAC
  601 CATTTTCCAG TCCCGGGAAA TTTGGAACTG GTACAGCGGC AAAGCAGTTT ACAGGGTTTT
  661 GATCAACTTT TATTTTCTTC ATTAACTTCT CTTTCCCCTC ATTAACTTCT ATTGAAAAAC
  721 CACAAGCCAA CTTGGTCACA TTGTTTTTAA CCAAAAATAC TGTGACTTGG AAATCGAGTC
  781 GCCTGCGGCT GAGCTGAGTT TTGGACATCT GGAGTTCACC ACCTCGCTTT CCCTATGAGA
  841 CACACAGGTG AGGTTTCGTT ATGGCCAGCA TGAGGGGCTG GCGGTCTCTG GAAAGCCTCG
  901 GGGGGGGGGG GGGGGGGGCT GCATTCCTGT GTCTCAAAGC CAGCACACTC AGAACATGAA
  961 CTCCCTCGGG GTCACTATTT TTTTGGACAC CAGCTAAGAT TGCTCAATAT GATACTGGGT
 1021 TTGTTTTTAA GGGAATCCAA AAAGGCTTTT GTGTGGGTGA AAAATGTGTG ATGGATGCTC
1081 CAACTTCTGC ATCCCGATTT TGATTTTCTC CACTTGGTGG ACCCTGA
//
Primer: R4-M_R RDB07877_A4Gic.seq
>07877_07877_A4Gi_R4-M_R_F05_17.ab1
    1 AAGATCGATT TGTTTTTCTC ACTTTGTGGC CCTGGAGGTT ACTGCATCCC TCTGGGGTAT
   61 GAAAAGCCAT GATACAGTGC TCAGACACAG ACCAGAATTA CTCAGCTTCT GCTGTTGGGC
  121 TGCCCAGGAC ATTTCAAAGA CCAGAGAACT GTAAACAGGT ATTTATCAAA AGCTTAGGTC
  181 TACGGGGAGG TGGAGGCAGG AGGATTGAGA GTTCAAGGCC ATCTTCTGCC TCGTGTTGAA
  241 TTCAAGGCCA GCAGGCAGGT TTGAAAGAAA AAAAGAATTT TCAAATGGAA TACTATGAAA
  301 TTAAAAGGCT TCAAACTGAG TGTGGTGACT AATCCTAGCA GCCTGGAGGT TGAGGCAGGA
  361 GGATTGTCAA GAGTTGGAAA CCACCTTGAG CTCCATCCCA GGACCCACAT GGTAGGGGGA
  421 GTGAACCAAT TCATGCAAGT TGTCCACTGA CCTCTACGCT CATACACACA CACACTCATA
  481 CACACACCCA TAATACACAC TCACACACAC TCATACACAC ACCCATACAC ACACTCATAC
  541 ACACACACAC CCACACACAC ACCCATACAT ACACACTCAC ATATACTCAT ACACCCTCAT
  601 CCCCACACCC ATATACACAC ACTCACACAC ACTCATACAC ACACTCATAC ACACACACCT
  661 CACACCCATA CATACACACT CACATACACT CATATACCCT CACCCCCCCA TATACACACA
  721 CTCACACACA CTCATACACA CACTCATACA CACACTCATA CACACACACA CCTCACACCC
  781 ATACATACAC ACTCACATAC ACTCATATAC TCTCACCCCC CCCCACACCC ATATACACAC
  841 ACTCACATAC ACTCATACAG CCCGAGGTTA CAGAGAGTTC CAGAACAGCC GAGAGTGGTG
  901 GGTACCTATC GATAGAGAAA TGTTCTGGCA CCTGCACTTG CACTGGGGAC AGCCTATTTT
  961 GCTAGTTTGT TTTGTTTCGT TTTGTTTTGA TGGAGAGCGT ATGTTAGTAC TATCGATTTC
 1021 ACACAAAAAA ACCCAACACA CAGATGGTAT GAAAATAAGA TATTTTAATG GCCGCGGCTC
 1081 TAGTACCTCC CGTAGTTAGT ATACTACTAT CATGCTAGCC TTGAGCTGGG GGCGGCTATA
1141 G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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