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pGL3-AID R4 del.-01

Reporter construct of mouse Aicda locus, position -9226 to -7585.

Catalog number RDB07855
Resource name pGL3-AID R4 del.-01
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -7585.
Nat. Immunol. (2010) 2, 148-154, Fig. 3a, b, c.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07855 pGL3-AID R4 del.-01 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content B cell receptor signaling pathway (English text)

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07855_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: R4-M_R RDB07855_A4Gia.seq
>07855_07855_A4Gi_R4-M_R_D04_10.ab1
    1 CAGGAATCGG ATTTGTTTTT CTCACTTTGT GGCCCTGGAG GTTACTGCAT CCCTCTGGGG
   61 TATGAAAAGC CATGGGACAG CCCTCAGACA CAGACCAGAA TTACTCAGCT TCTGCTGTTG
  121 GGCTGCCCAG GACATTTCAA AGACCAGAGA ACTGTAAACA GGTATTTATC AAAAGCTTAG
  181 GTCTACGGGG AGGTGGAGGC AGGAGGATTG AGAGTTCAAG GCCATCTTCT GCCTCGTGTT
  241 GAATTCAAGG CCAGCAGGCA GGTTTGAAAG AAAAAAAGAA TTTTCAAATG GAATACTATG
  301 AAATTAAAAG GCTTCAAACT GAGTGTGGTG ACTAATCCTA GCAGCCTGGA GGTTGAGGCA
  361 GGAGGATTGT CAAGAGTTGG AAACCACCTT GAGCTCCATC CCAGGACCCA CATGGTAGGG
  421 GGAGTGAACC AATTCATGCA AGTTGTCCAC TGACCTCTAC GCTCATACAC ACACACACTC
  481 ATACACACAC CCATAATACA CACTCACACA CACTCATACA CACACCCATA CACACACTCA
  541 TACACACACA CACCCACACA CACACCCATA CATACACACT CACATATACT CATACACCCT
  601 CATCCCCACA CCCATATACA CACACTCACA CACACTCATA CACACACTCA TACACACACA
  661 CCTCACACCC ATACATACAC ACTCACATAC ACTCATATAC CCTCACCCCC CCATATACAC
  721 ACACTCACAC ACACTCATAC ACACACTCAT ACACACACTC ATACACACAC ACACCTCACA
  781 CCCATACATA CACACTCACA TACACTCATA TACTCTCACC CCCCCCACAC CCATATACAC
  841 ACACTCACAT ACACTCATAC AGCCCGAGGT TACAGAGAGT TCCAGAACAG CCGAGAGTGG
  901 TGGGTACCTA TCGATAGAGA AATGTTCTGG CACCTGCACT TGCACTGGGG GACAGCCTAT
  961 TTTGCTAGTT TGTTTTGTTT TCGTTTTGTT TTGATGGGAG AGCGTATGTT AGTACTATCG
 1021 ATTCACACAA AAAAAACCCA ACACACAGAT GTAAATGAAA AAATAAGGAA TATTTTATTG
 1081 CGGGCCGCTC GAAGTTACCC TTCCCGGTAC TAATATTACT TAACTTATCA TGGATAAGCT
1141 TGGGGGCCTT GGGGCCG
//
Primer: pGL3_RV6R RDB07855_A4Gib.seq
>07855_07855_A4Gi_pGL3_Rv6R_D12_12.ab1
    1 GTAAATCTTT CAGCGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCCA
   61 TGGATCGGTC TCCAGCGTGA CTTTCTTGAC TCCCTCAAAG TCTTAGGCCA AAGGAAGCTT
  121 GCTTCAAGCA AGGCTGCTTC AGTGCTGTTG TGTGACAGAG CACTGGCCCC TGGCATTCAA
  181 ATCTGAGCTT GCTCTTGTGG GCTCCTCCCC CACGACAGCA TCACTACCAC CAGGTATCCA
  241 ACATCTGCCT CCTGGGTTGG GTCCAGTAAA GATGGGCGGG GCTGTGGTGG GCAAAGCCAC
  301 ACGGATTAAA TGATTGGGGG GGGGGGCCAG TTCTGCTCAT GCTTAATGTC CAACTTCCTT
  361 CGAGGCTGTT CCTCTAAACT CGAGCTGTAG CACACGCACA CGCACGCCCA CACCCTCTCT
  421 TACAGATGCC GTACACATGC CATAGGAACA AATACAAAAC CCTTGATTTG TAAGAAAGCC
  481 AAATTCTCTC ATTTGCCTTC TTCTAAAGGA AGGAAAGAGA TCTCACTTAG TTACAAAAAC
  541 CATTTTCCAG TCCCGGGAAA TTTGGAACTG GTACAGCGGC AAAGCAGTTT ACAGGGTTTT
  601 GATCAACTTT TATTTTCTTC ATTAACTTCT CTTTCCCCTC ATTAACTTCT ATTGAAAAAC
  661 CACAAGCCAA CTTGGTCACA TTGTTTTTAA CCAAAAATAC TGTGACTTGG AAATCGAGTC
  721 GCCTGCGGCT GAGCTGAGTT TTGGACATCT GGAGTTCACC ACCTCGCTTT CCCTATGAGA
  781 CACACAGGTG AGGTTTCGTT ATGGCCAGCA TGAGGGGCTG GCGGTCTCTG GAAAGCCTCG
  841 GGGGGGGGGG GGGGCTGCAT TCCTGTGTCT CAGAGCCAGC ACACTCAGAC ATGACTCCTC
  901 GGGTCACTAT TTTTGGACAC CAGCTAGATT GCTCAATATG ATACTGGTTG TTTTAAGGGG
  961 ATCCAAAAAG GGCTTGTGTG GGTGAAATGT GGTTGTGGGA TTGCTCACTC TGCAATCCCC
 1021 GATTTTGTTT TTCTCACTTG TGACCTTGAG TACTGCATCC CTCTGGGATG GAAGGCAATG
1081 GACAGCCTCG ACCCGACCGA ATACTCAGCT TCTGCCGT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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