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pGL3-AID R2 del.-03

Reporter construct of mouse Aicda locus, position +639 to +1789.

Catalog number RDB07832
Resource name pGL3-AID R2 del.-03
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, +639 to +1789.
Nat. Immunol. (2010) 2, 148-154, Fig. 2b.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07832 pGL3-AID R2 del.-03 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07832_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ME-1290Rv RDB07832_A4Gia.seq
>07832_07832_A4Gi_ME_1290Rv_B12_06.ab1
    1 ATGGCATTTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
   61 TAAAACCTCT ACAAATGTGG TAAAATCGAT AAGGGATCCG CAGGGTGTTC TTCAGATTCC
  121 AGATTTTTCC TTTCTTGTCT TCCTTCTTTC TTTTTGTTAC CCTTCTCCTA CCCCCTCTTC
  181 TTCTCCCCCT CCTCCTCTTC CTCCCTCCTC CCCATCTCTT CCCCTCCTCT TCTCCCTCCT
  241 CCCCCTCCTC TTCCCCTTCT CCTCTTCCCC CTCTTCCTCC TCTTCCTCCT CCTTCTCCTG
  301 CTCCCCATCT CTTTCCCTCC TCTTCTCCCT CCTCCTCCTC CTTTTGCCCC TTCTCCTCTT
  361 CCCCCTCCTC CTCTTCCTTT TCCTTCTCCT CCTTCTCCTC CCCCCTCTCC TCCCCCTTTC
  421 CTCCTCCCAC TCTCCCTCAC CCCTATCAGG GACCACATTG AAGACCTCAC ACATGCTAGA
  481 CAAGTAATCT GCCACTTAAT TACATCCTGA GCCCTCAAAA AGCAAACAGA CAGACAGACA
  541 GACAGACAGA CAGACAGACA AACAAACAAA TGTTCACAGG AGGCAGGCAG ACAGCCTGAG
  601 CTGCTTCTGG GTTTATAGTG AATTTTGAAA CCAAATCTGA GATCTATGTC CTGATGGAGA
  661 AGGGTCCGAG AGAAATGCAT GAGCATGGCA AAATGCAAAG CAAAGACGAG GCTGAGATTC
  721 AGGGAGAAGC AAACAAGACA GTGGAGAGAC ACAGGATGGC ACGGCACGGA CTGGAGCAAG
  781 GGCAGCGAGT AACTCAAGGC AGCCCTGCTA CTAGGCTGGG ATTATTTTTA ACCCCTTGAG
  841 TCTGGTTTGC ATTGCTGGGG AAGCAGCTAA GGTTCTGCCT CAAGGAGCAC AGCTGTCTCA
  901 GCAGCTGGCG ATCTACAGGT TTGGGACACC ACCTAGCAAA GTCCCTCCCT TACCGGGAGG
  961 GACATCCCCG AGGAGAGGGA GCTGGAAATA GGCTCCTAGC TAGAGTTGAG GGGGGATCCG
 1021 TCGACCGATG CCCTTTGAGA GCCTTTCAAC CCAGTCAGCT CCTTCCTGGT GGGGCGCGGG
 1081 GGCATGACTA TCGTCGCCCG CACTTATGAC TGTCATTCGT TATCATGCAA CTTCGTAAGA
1141 CAGATGCCCG GCAGCGCCTT CTTTTACCGG CCATTGCGCT GTCGGCTTTC AACT
//
Primer: pGL3_RV4 RDB07832_A4Gib.seq
>07832_07832_A4Gi_Rv4_C12_09.ab1
    1 TCAGGACTGA TGGGTTGAGG CTCTCAGGGC ATCGGTCGAC GGATCCCCCC TCAACTCTAG
   61 CTAGGAGCCT ATTTCCAGCT CCCTCTCCTC GGGATGTCCC TCCCGGTAAG GAGGACTTTG
  121 CTAGGTGGTG TCCCAAACCT GTAGATCGCC AGCTGCTGAG ACAGCTGTGC TCCTTGAGGC
  181 AGAACCTTAG CTGCTTCCCC AGCAATGCAA ACCAGACTCA AGGGGTTAAA AATAATCCCA
  241 GCCTAGTAGC AGGGCTGCCT TGAGTTACTC GCTGCCCTTG CTCCAGTCCG TGCCGTGCCA
  301 TCCTGTGTCT CTCCACTGTC TTGTTTGCTT CTCCCTGAAT CTCAGCCTCG TCTTTGCTTT
  361 GCATTTTGCC ATGCTCATGC ATTTCTCTCG GACCCTTCTC CATCAGGACA TAGATCTCAG
  421 ATTTGGTTTC AAAATTCACT ATAAACCCAG AAGCAGCTCA GGCTGTCTGC CTGCCTCCTG
  481 TGAACATTTG TTTGTTTGTC TGTCTGTCTG TCTGTCTGTC TGTCTGTCTG TTTGCTTTTT
  541 GAGGGCTCAG GATGTAATTA AGTGGCAGAT TACTTGTCTA GCATGTGTGA GGTCTTCAAT
  601 GTGGTCCCTG ATAGGGGTGA GGGAGAGTGG GAGGAGGAAA GGGGGAGGAG AGGGGGGGAT
  661 CCAGAAGGAC GATGACCCTA AAGAGCCATG CAGGAGAGGT CAAGCACCGA TCACGGTGGC
  721 AAACGGAGGC ATGAGAGGGA GAAGAGGAGG GAAAGAGATG GGGAGCAGGA GAAGAAGGAG
  781 AGAGGAGGAG AGGGGGAAGA GGAGAAGGGA AGAGAGGGGA GGAAGGAGAA GAGGAGGGAA
841 GAAATGGGAA GGAGGGAAGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

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