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pGL3-AID R1 del.-03

Reporter construct of mouse Aicda locus, position -1075 to +101.

Catalog number RDB07826
Resource name pGL3-AID R1 del.-03
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -1075 to +101.
Nat. Immunol. (2010) 2, 148-154, Fig. 1a.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07826 pGL3-AID R1 del.-03 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07826_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07826_A4Gia.seq
>07826_07826_A4Gi_pGL4-4174F_B11_05.ab1
    1 AGGAAGGTCA GACATTTCTC TATCGATAGG TACCGAGCTC TTACGCGTGC TAGCCCGGGC
   61 TCGAGAGACC AGCCAGAGGA GCTACATAAT GACACCCTAT TTTAAAAAAG GAAGGGAAGG
  121 AAGGAAGGAA GGAAGGAAGG AAGGAAGGAA GGAAGGAAGC AAAGGATGTT CTTCCAGATG
  181 ATCAGGGTTC AGATCCCAGC AAGCACACAT GGTGGCTTTC AACTGCCTGC TGGTCCTCTG
  241 CAATAGAAGT AAGTGCTCTT AATCACTGGG CCAGCTCTCC AGGCCTCCAG TAAGGTATTT
  301 TTAATGAGGA AAAGGAGTTC TTTTTTTTTT TAATACTTTT TGACACACAC ACACACACAA
  361 AATTAAAATA AATCACTTTT TGGTGCAAGC AACTAGTCTT TCTAGCTATC TTATAATGTC
  421 ATTTTAAAAA AAGAAAAATA TATTAGAGAA TTGGGAGGCT AAAGTTCACT CTCTGGATGC
  481 TGTGGTGGTC AACCCCCATC TCTACTGAGG CATAAAACTG AGTGTAACAA ACGGAAGGAA
  541 CAGATACTGT AAGTTCAAGA AGCACAAGAT GCAACTGAAG TGCCATTTAA GGCCACTTTA
  601 AGTCACTATG ACTGCTATCA TTCTTGTTAT CATAGTTTTA AAATTAGGAA GTATGCACAG
  661 ACCTTAGGTG TGATACCCGG GACCACACAC ACACACACAC ACACTCACAG AGCTCATTAT
  721 CATGATACCA ATGTGAGAAG TGTCCAGTGC TATTGTCTCC TGATCTTTGT TACCTGTGAT
  781 ACCTGGGCTG GCTTTTTAGA GGAACAGCCT CGAAGGAAGT TGGACATTAA GCATGAGCAG
  841 AACTGGCCCC CCCCCCCAAT CATTTAATCC GTGTGGCTTT GCCCACCACA GCCCCGCCCA
  901 TCTTTACTGG ACCCAACCCA GGAGGCAGAT GTTGGATACC TGGTGGTAGT GATGCTGTCG
  961 TGGGGGAGGA GCCCACAAGA GCAAGCTCAG ATTTGAATGC CAGGGGCCAG TGCTCTGTCA
 1021 CACAACAGCA CTGAAGCAGC CCTTGCTTGA AGCAAGCTTC CTTTGGCTAG ACCTTGAGGG
 1081 AGTCCAAGAA AGTTCACGCT GGAGACGATC CATGCTAGAA CGCAAAACAT AATGACGACC
1141 GGCGCATTCT ATCGCTGCAA GAATGGGAAC CGCCTTG
//
Primer: Luc_R RDB07826_A4Gib.seq
>07826_07826_A4Gi_Luc_R_C11_08.ab1
    1 CATCGTTTCA GGAACAGGGC GTATCTCTTC ATAGCCTTAT GCAGTTGCTC TCCAGCGGTT
   61 CCATCTTCCA GCGGATAGAA TGGCGCCGGG CCTTTCTTTA TGTTTTTGGC GTCTTCCATG
  121 GATCGGTCTC CAGCGTGACT TTCTTGACTC CCTCAAAGTC TTAGGCCAAA GGAAGCTTGC
  181 TTCAAGCAAG GCTGCTTCAG TGCTGTTGTG TGACAGAGCA CTGGCCCCTG GCATTCAAAT
  241 CTGAGCTTGC TCTTGTGGGC TCCTCCCCCA CGACAGCATC ACTACCACCA GGTATCCAAC
  301 ATCTGCCTCC TGGGTTGGGT CCAGTAAAGA TGGGCGGGGC TGTGGTGGGC AAAGCCACAC
  361 GGATTAAATG ATTGGGGGGG GGGGCCAGTT CTGCTCATGC TTAATGTCCA ACTTCCTTCG
  421 AGGCTGTTCC TCTAAAAAGC CAGCCCAGGT ATCACAGGTA ACAAAGATCA GGAGACAATA
  481 GCACTGGACA CTTCTCACAT TGGTATCATG ATAATGAGCT CTGTGAGTGT GTGTGTGTGT
  541 GTGTGTGGTC CCGGGTATCA CACCTAAGGT CTGTGCATAC TTCCTAATTT TAAAACTATG
  601 ATAACAAGAA TGATAGCAGT CATAGTGACT TAAAGTGGCC TTAAATGGCA CTTCAGTTGC
  661 ATCTTGTGCT TCTTGAACTT ACAGTATCTG TTCCTTCCGT TTGTTACACT CAGTTTTATG
  721 CCTCAGTAGA GATGGGGGTT GACCACCACA GCATCCAGAG AGTGAACTTT AGCCTCCCAA
  781 TTCTCTAATA TATTTTTCTT TTTTTAAAAT GACATTATAA GATAGCTAGA AAGACTAGTT
  841 GCTTGCACCA AAAAGTGATT TATTTTAATT TTGTGTGTGT GTGTGTGTCA AAAAGTATTA
  901 AAAAAAAAAA GAACTCCTTT TTCCTCATTA AAAATACCTT ACTGGAGGCC TGGAGAGCTG
  961 GCCCAGTGAT TAAGAGCACT TACTTCTATT GCAGAGGGAC CAGCAGGCAG TTGAAAGCCA
 1021 CCATGTGTGC TTTGCTGGGA TCTGACCTGA TCATCTGCAG ACATTCGTTG CTCTCTCCAT
1081 TTCCCTCGTA CATACTCCAT CGATCCGTCC GTTCGTTTTT ATTTAAAAAA TACGGGCGC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl