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pGL3-AID R1 del.-02

Reporter construct of mouse Aicda locus, position -1223 to +101.

Catalog number RDB07825
Resource name pGL3-AID R1 del.-02
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -1223 to +101.
Nat. Immunol. (2010) 2, 148-154, Fig. 1a.
Vector backbone PGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Aicda (NCBI Gene 11628) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07825 pGL3-AID R1 del.-02 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07825_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07825_A4Gia.seq
>07825_07825_A4Gi_pGL4-4174F_H10_22.ab1
    1 AGCAGGGTTC AGACATTTCT CTATCGATAG GTACCGAGCT CTTACGCGTG CTAGCCCGGG
   61 CTCGAGCTGC CAGGGAGTAA GCCAAGGCCT GAACTATGAG ACTTTGCACA GAGAGATTTC
  121 ACAGCAACAA AATAGGGGCA GGGGCATGTG CTGTGTGCAT GCAACGGGAT CCAGTCTCTA
  181 GCTCAAGACT GGTCTGGTCT ATATAGAAAG TTCCAGACCA GCCAGAGGAG CTACATAATG
  241 ACACCCTATT TTAAAAAAGG AAGGGAAGGA AGGAAGGAAG GAAGGAAGGA AGGAAAGAAG
  301 GAAGAAGGAA GGAAGAAGGG AGGGAGGGAG GGAGGGAGGG AGGGAGGGAG GAAGGAAGGG
  361 AGGGAGGGAG GGAGGGAGGG AGGGAGAGAG GGAAGGAGGG AGGGAGAGAG AGGGGAGGAA
  421 GGAAGAAGGA AGGAAGGAAG AAAGGAAGGA AGGGAGGGAG GAAAGAAGGA AGGAAGGGAG
  481 GGAGGAAAGA AGGAAGGAAG GAAGGAAGGA AGGAAGGAAG GAAGGAAGGA AGGAAGGAAG
  541 GAAGGACGGA ATCAAATGAC GCCCTTCCAG ATGATCACAG CTCATATCCC AGCCAGCCAG
601 CAGGAGGCTA CTCACCCGCC TGCTCATCCT CTGCCA
//
Primer: Luc_R RDB07825_A4Gib.seq
>07825_07825_A4Gi_Luc_R_A11_02.ab1
    1 GTCTGCGCAG GACAGGGCGT ATCTCTTCAT AGCCTTATGC AGTTGCTCTC CAGCGGTTCC
   61 ATCTTCCAGC GGATAGAATG GCGCCGGGCC TTTCTTTATG TTTTTGGCGT CTTCCATGGA
  121 TCGGTCTCCA GCGTGACTTT CTTGACTCCC TCAAAGTCTT AGGCCAAAGG AAGCTTGCTT
  181 CAAGCAAGGC TGCTTCAGTG CTGTTGTGTG ACAGAGCACT GGCCCCTGGC ATTCAAATCT
  241 GAGCTTGCTC TTGTGGGCTC CTCCCCCACG ACAGCATCAC TACCACCAGG TATCCAACAT
  301 CTGCCTCCTG GGTTGGGTCC AGTAAAGATG GGCGGGGCTG TGGTGGGCAA AGCCACACGG
  361 ATTAAATGAT TGGGGGGGGG GGGCCAGTTC TGCTCATGCT TAATGTCCAA CTTCCTTCGA
  421 GGCTGTTCCT CTAAAAAGCC AGCCCAGGTA TCACAGGTAA CAAAGATCAG GAGACAATAG
  481 CACTGGACAC TTCTCACATT GGTATCATGA TAATGAGCTC TGTGAGTGTG TGTGTGTGTG
  541 TGTGTGTGTG GTCCCGGGTA TCACACCTAA GGTCTGTGCA TACTTCCTAA TTTTAAAACT
  601 ATGATAACAA GAATGATAGC AGTCATAGTG ACTTAAAGTG GCCTTAAATG GCACTTCAGT
  661 TGCATCTTGT GCTTCTTGAA CTTACAGTAT CTGTTCCTTC CGTTTGTTAC ACTCAGTTTT
  721 ATGCCTCAGT AGAGATGGGG GTTGACCACC ACAGCATCCA GAGAGTGAAC TTTAGCCTCC
  781 CAATTCTCTA ATATATTTTT CTTTTTTTAA AATGACATTA TAAGATAGCT AGAAAGACTA
  841 GTTGCTTGCA CCAAAAAGTG ATTTATTTTA ATTTTGTGTG TGTGTGTGTG TCAAAAAGTA
  901 TTAAAAAAAA AAAAAAGAAC TCCTTTTCCT CATTAAAAAT ACCTTTACTG GGAGGCCCTG
  961 GAGAGCTGGC CCAGTGATTA AGAGCACTTT ACTTCTATTG CAGAGGACCA GCAGGCAGTT
 1021 TGAAAGCCAC CATGTGTGCT TTGCTGGATC TGACCTGATC ATCTGGAGAC ATCGATGCTC
1081 TCTCTTCGTT CCTTCCGTTA CATTACATTA CTTACGTACG TTCCGTTCCG TTCGTTGCT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl