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pGL4-phETS1 (#RDB07819)

Promoter collection, Human ETS1 promoter

Alternative name U3027-3
Clone info. PCR amplified human ETS1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 128523650 to 128522176(NC_000011.10 ); relatively +63908 to +65383, where +1 corresponds to 1 nt of NM_001143820.2; relatively -1340 to +135, where +1 corresponds to 1 nt of NM_005238.4.
Comment PCR cloning, forward primer:atttgctgtgggaaatctgc; reverse primer: aggggaagttggcactttg.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) genomic DNA
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RDB07819 pGL4-phETS1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phETS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07819).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07819_A5F4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07819_A5F4a.seq check
>07819_07819_A5F4_2_pGL4-4174F_A01_01.ab1
    1 CTGGGTCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
   61 ATTTGCTGTG GGAAATCTGC AAATTACTAA GGCTTCAAGG GCACAAAACT GGGATTAGAT
  121 GAAGGTAAGC CTGTGTGTCA AAACTTACCA GAGGCAGAGG TTACAGTTAC AGTCAAATGC
  181 TGGGAGTTCA AATGTGCTTA GAAATCAGGA AACATTAAAA ATAGCTTTCT CTGAGATTGC
  241 TCAGGAAGAT TTATGTTTAA TCTTTCTGTT AATCAAAGAT GGAGCTCAGT GCCTGGTTCT
  301 AGCTATGTAT TTAGATATAT ACATGTACTG GTTGATTTGT TAATTAGGAC AAACTATCTC
  361 TATTACAACC AATGTATTAG TGGCAAACCA TGAATTATTC GGGTTCATTT TGTTTTATCT
  421 CTAGTGTTGG GGGTTCCCAA ATGGAACAGA AACTGTGCAT TGTGATTCTG TCATGGCTGC
  481 CAATGATGTA CTCCAGAAAT TCAAAACCAC AGAGCCTCTG CATCACTTTT TTGGTGGCGG
  541 TCGTGGGAGG GTTGTTAGTT TTATCTAAAA GAGGAGAGTC TGTGAAAGGA AAGACAAAGC
  601 TTTGGCCAAT GGTGACATAA TTAGAGGAAC AGAATAATTA GAAATAAATC ATAACTTCCC
  661 CTTCAACCCA ACCACTGCTG GAGGTAAATT GGAAGCTTAC GGAAGCTCAT GGCTGGGTTC
  721 TAGTTTGGGG TCCCGTTCTG GACTCCCCTG CCAACGCAGG AGCATTACAT GGGTGGGCTC
  781 TGAAAAACTG AAGCCTCCTT CAGAAACACA CGCCCTGACT CAAGATCCGG CTGGAGTCCA
  841 ATACTCCTAA AGCCCTTTGA GGACACGGGC TCACGAATCC CCTGCGCCTG CCTGCACGCT
  901 CGCCTTCATC CACATGCCTC ACGTCCTGTG TGTCAGTCTT TGTGAAATGA ATGATGTACA
  961 CGCACTTGGA AAACTATGCT GCTACTGGGA GGGGGGCGAG AGCGGGTGAC CCAAGCCCTC
 1021 AAGGAATTGC GTGAGGATCA AGACGGACTT TCCGGAAACC GCTGAGCCGT CTGTGCACCA
1081 GCCTGCACAC CCGCTCCCTG GCCGTTTCCC CGACCCCCCG
//
Primer: pGL4-136R
Sequence file: RDB07819_A5F4b.seq check
>07819_07819_A5F4_2_pGL4-136R_B01_04.ab1
    1 GCTAAGCTTT TGGCATCTTC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC
   61 CGCCGAGGCC AGATCTTGAT AGGGGAAGTT GGCACTTTGC GGCGAAGTGA GCGCGCTCGG
  121 GTCCCAGCCT CGCCCGCGCC GCGCCCGCTC CTCCTGCCCG GCCCTCGCCC GCTCCCTCCT
  181 CTCCGCCGGC GGCTGCCTCG TTCGCTCTCG ATCTCCCGGC CGCTCTCCCT CCCTCTCGCC
  241 CTCCCGCGCT CTCCCCTCCT CTTTAGGGCG TTTCTCGCGG CGCCGCGTCT CGGCCGCTGG
  301 GTCCGCGCGC CCTGGGCCGG GCGATGTCCG CTTGGGGGAG CGAGGGGCGG GGCGTCGGGG
  361 CAGGGCGGGG AGCCGGGGGC GGGGCCGAGC ACCGCGGCCA ATCTCCGCCC GCGGCCCAAA
  421 GCGAAAGGAA GGGCTGGCGA GCGCGGGGCC GGGCCGCGAC CGCGGAGGAG CAGTGCGTGG
  481 AGCCCCGGGC CTCGGAGCCC AGGCTGGGGC GGGAAGGGCC GGGAGCGGGT GTGCAGGCTG
  541 GGTGCACAGG CCGCCTCCAC CGTTTCGGGA AAGTCCGTCT GATTCTCCAC GCATTCTTGA
  601 GGGCTTGGTC ACCCGCTCTC GCCCCCTCCC AGTAGCAGCA TAGTTTTCCA AGTGCGTGTA
  661 CATCATTCAT TCACAAAGAC TGACACACAG GACGTGAGGC ATGTGGATGA AGGCGAGCGT
  721 GCAGGCAGGC GCAGGGGATT CGTGAGCCCG TGTCCTCAAA GGGCTTTAGG AGTATTGGAC
  781 TCCAGCCGGA TCTTGAGTCA GGGCGTGTGT TTCTGAAGGA GGCTTCAGTT TTTCAGAGCC
  841 CACCCATGTA ATGCTCCTGC GTTGGCAGGG GAGTCCAGAA CGGGACCCCA AACTAGAACC
  901 CAGCCATGAG CTTCCGTAAG CTTCCAATTT ACCTCCAGCA GTGGTTGGGG TTGAAAGGGG
  961 AAGTTATGAT TATTTCTAAA TTATTCTGTT CCTCTAATAT GTCACCATTG GCCAAAGCTT
 1021 GTCCTTCTTC ACAGACTCCT CTTCTTTAGA TAACTACAAC CATCCCACGA CGCACGAAAA
1081 GTGATGCAGA GCTCTGGTGG ATCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles