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pGL4-phETS1

Promoter collection, Human ETS1 promoter

Catalog number RDB07819
Resource name pGL4-phETS1
Alternative name U3027-3
Clone info. PCR amplified human ETS1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 128523650 to 128522176(NC_000011.10 ); relatively +63908 to +65383, where +1 corresponds to 1 nt of NM_001143820.2; relatively -1340 to +135, where +1 corresponds to 1 nt of NM_005238.4.
Comment PCR cloning, forward primer:atttgctgtgggaaatctgc; reverse primer: aggggaagttggcactttg.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
          Reference sequence
            
           

          Distribution information

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          RDB07819 pGL4-phETS1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phETS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07819).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07819_A5F4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07819_A5F4a.seq check
          >07819_07819_A5F4_2_pGL4-4174F_A01_01.ab1
              1 CTGGGTCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
             61 ATTTGCTGTG GGAAATCTGC AAATTACTAA GGCTTCAAGG GCACAAAACT GGGATTAGAT
            121 GAAGGTAAGC CTGTGTGTCA AAACTTACCA GAGGCAGAGG TTACAGTTAC AGTCAAATGC
            181 TGGGAGTTCA AATGTGCTTA GAAATCAGGA AACATTAAAA ATAGCTTTCT CTGAGATTGC
            241 TCAGGAAGAT TTATGTTTAA TCTTTCTGTT AATCAAAGAT GGAGCTCAGT GCCTGGTTCT
            301 AGCTATGTAT TTAGATATAT ACATGTACTG GTTGATTTGT TAATTAGGAC AAACTATCTC
            361 TATTACAACC AATGTATTAG TGGCAAACCA TGAATTATTC GGGTTCATTT TGTTTTATCT
            421 CTAGTGTTGG GGGTTCCCAA ATGGAACAGA AACTGTGCAT TGTGATTCTG TCATGGCTGC
            481 CAATGATGTA CTCCAGAAAT TCAAAACCAC AGAGCCTCTG CATCACTTTT TTGGTGGCGG
            541 TCGTGGGAGG GTTGTTAGTT TTATCTAAAA GAGGAGAGTC TGTGAAAGGA AAGACAAAGC
            601 TTTGGCCAAT GGTGACATAA TTAGAGGAAC AGAATAATTA GAAATAAATC ATAACTTCCC
            661 CTTCAACCCA ACCACTGCTG GAGGTAAATT GGAAGCTTAC GGAAGCTCAT GGCTGGGTTC
            721 TAGTTTGGGG TCCCGTTCTG GACTCCCCTG CCAACGCAGG AGCATTACAT GGGTGGGCTC
            781 TGAAAAACTG AAGCCTCCTT CAGAAACACA CGCCCTGACT CAAGATCCGG CTGGAGTCCA
            841 ATACTCCTAA AGCCCTTTGA GGACACGGGC TCACGAATCC CCTGCGCCTG CCTGCACGCT
            901 CGCCTTCATC CACATGCCTC ACGTCCTGTG TGTCAGTCTT TGTGAAATGA ATGATGTACA
            961 CGCACTTGGA AAACTATGCT GCTACTGGGA GGGGGGCGAG AGCGGGTGAC CCAAGCCCTC
           1021 AAGGAATTGC GTGAGGATCA AGACGGACTT TCCGGAAACC GCTGAGCCGT CTGTGCACCA
          1081 GCCTGCACAC CCGCTCCCTG GCCGTTTCCC CGACCCCCCG
          //
          Primer: pGL4-136R
          Sequence file: RDB07819_A5F4b.seq check
          >07819_07819_A5F4_2_pGL4-136R_B01_04.ab1
              1 GCTAAGCTTT TGGCATCTTC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC
             61 CGCCGAGGCC AGATCTTGAT AGGGGAAGTT GGCACTTTGC GGCGAAGTGA GCGCGCTCGG
            121 GTCCCAGCCT CGCCCGCGCC GCGCCCGCTC CTCCTGCCCG GCCCTCGCCC GCTCCCTCCT
            181 CTCCGCCGGC GGCTGCCTCG TTCGCTCTCG ATCTCCCGGC CGCTCTCCCT CCCTCTCGCC
            241 CTCCCGCGCT CTCCCCTCCT CTTTAGGGCG TTTCTCGCGG CGCCGCGTCT CGGCCGCTGG
            301 GTCCGCGCGC CCTGGGCCGG GCGATGTCCG CTTGGGGGAG CGAGGGGCGG GGCGTCGGGG
            361 CAGGGCGGGG AGCCGGGGGC GGGGCCGAGC ACCGCGGCCA ATCTCCGCCC GCGGCCCAAA
            421 GCGAAAGGAA GGGCTGGCGA GCGCGGGGCC GGGCCGCGAC CGCGGAGGAG CAGTGCGTGG
            481 AGCCCCGGGC CTCGGAGCCC AGGCTGGGGC GGGAAGGGCC GGGAGCGGGT GTGCAGGCTG
            541 GGTGCACAGG CCGCCTCCAC CGTTTCGGGA AAGTCCGTCT GATTCTCCAC GCATTCTTGA
            601 GGGCTTGGTC ACCCGCTCTC GCCCCCTCCC AGTAGCAGCA TAGTTTTCCA AGTGCGTGTA
            661 CATCATTCAT TCACAAAGAC TGACACACAG GACGTGAGGC ATGTGGATGA AGGCGAGCGT
            721 GCAGGCAGGC GCAGGGGATT CGTGAGCCCG TGTCCTCAAA GGGCTTTAGG AGTATTGGAC
            781 TCCAGCCGGA TCTTGAGTCA GGGCGTGTGT TTCTGAAGGA GGCTTCAGTT TTTCAGAGCC
            841 CACCCATGTA ATGCTCCTGC GTTGGCAGGG GAGTCCAGAA CGGGACCCCA AACTAGAACC
            901 CAGCCATGAG CTTCCGTAAG CTTCCAATTT ACCTCCAGCA GTGGTTGGGG TTGAAAGGGG
            961 AAGTTATGAT TATTTCTAAA TTATTCTGTT CCTCTAATAT GTCACCATTG GCCAAAGCTT
           1021 GTCCTTCTTC ACAGACTCCT CTTCTTTAGA TAACTACAAC CATCCCACGA CGCACGAAAA
          1081 GTGATGCAGA GCTCTGGTGG ATCG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.11.09

          GNP_filter3_RDBDEP_html_231027.pl