RDB07818 - pGL4-phALB

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pGL4-phALB (#RDB07818)

Promoter collection, Human ALB promoter

Alternative name	U2911-5
Clone info.	PCR amplified human ALB promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 73402814 to 73404284(NC_000004.12); relatively -1473 to -2, where +1 corresponds to 1 nt of NM_000477.7.
Comment	PCR cloning, forward primer:tcctttcaggactcccttga; reverse primer: caaacggagggaaattagca.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human albumin genomic DNA
Depositor\|Developer	DNA Bank, \|

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07818	pGL4-phALB	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phALB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07818).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07818_A3Gap1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07818_A3Gaa.seq
>07818_07818_A3Ga_pGL4-4174F_G07_19.ab1 1 ACGGGTCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT 61 TCCTTTCAGG ACTCCCTTGA GTCATTAAAA AAAATCTTCC TATCTATCTA TGTATCTATC 121 ATCCATCTAG CTTTGATTTT TTCCTCTTCT GTGCTTTATT AGTTAATTAG TACCCATTTC 181 TGAAGAAAAA ATAACATAAG ATTATAGAAA ATAATTTCTT TCATTGTAAG ACTGAATAGA 241 AAAAATTTTC TTTCATTATA AGACTGAGTA GAAAAAATAA TACTTTGTTA GTCTCTGTGC 301 CTCTATGTGC CATGAGGAAA TTTGACTACT GGTTTTGACT GACTGAGTTA TTTAATTAAG 361 TAAAATAACT GGCTTAGTAC TAATTATTGT TCTGTAGTAT CAGAGAAAGT TGTTCTTCCT 421 ACTGGTTGAG CTCAGTAGTT CTTCATATTC TGAGCAAAAG GGCAGAGGTA GGATAGCTTT 481 TCTGAGGTAG AGATAAGAAC CTTGGGTAGG GAAGGAAGAT TTATGAAATA TTTAAAAAAT 541 TATTCTTCCT TCGCTTTGTT TTTAGACATA ATGTTAAATT TATTTTGAAA TTTAAAGCAA 601 CATAAAAGAA CATGTGATTT TTCTACTTAT TGAAAGAGAG AAAGGAAAAA AATATGAAAC 661 AGGGATGGAA AGAATCCTAT GCCTGGTGAA GGTCAAGGGT TCTCATAACC TACAGAGAAT 721 TTGGGGTCAG CCTGTCCTAT TGTATATTAT GGCAAAGATA ATCATCATCT CATTTGGGTC 781 CATTTTCCTC TCCATCTCTG CTTAACTGAA GATCCCATGA GATATACTCA CACTGAATCT 841 AAATAGCCTA TCTCAGGGCT TGAATCACAT GTGGGCCACA GCAGGAATGG GAACATGGAA 901 TTTCTAAGTC CTATCTTACT TGTTATTGTT GCTATGTCTT TTTTCTTAGT TTGCATCTGA 961 GGCAACATCA GCTTTTTTCA GACAGAAATG GCTTTGGAAA TAGTAAAAAA AGACACAGAA 1021 GCCCTAAAAA TATGTATGTA TGTATATGTG TGTGTGCATG CGTGGAGTAC TTGTGTGTAA 1081 ATTTTTCATA ATCTTATAGG TTAAAAGCAC ACTTGCAATT AGCAATAAGA ATGCCAATTT 1141 TG //
Primer: pGL4-136R Sequence file: RDB07818_A3Gab.seq
>07818_07818_A3Ga_pGL4-136R_H07_22.ab1 1 GTTCCTAAGT TTTGGCATCT TCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG 61 GCCGCCGAGG CCAGATCTTG ATCAAACGGA GGGAAATTAG CACTAATATA CTTCTTTAAC 121 CAATAATTGT AGATTATTAA CTAGACTCAT CTTTGTATTT CATTGGCTGC CAACCGATTA 181 CAAAATTCAT AATATTCTTG CCAAAAAATT GTTTGACTAA ATCCCTTGTG TATATTTACC 241 ATCTGGAACT GTTTTCTCCA CAAGACCTTG TCAATGTATT AAAGTTGTGT AAAACATCTT 301 ATCAAATTTT ACATAAGAGT TTAAACGATT TGTACCTTTT ATTCATTACC ATGGTATTTA 361 ACTGTAATTT CCCACTTGAA CATTTTAGTT CTTGGAGTTT GCAATTTAGT TCTCAGTGTT 421 TAGTAATTAC TAACCAAACT AAATACTTTG CTTAGAAATA AGACATACTG AAAGAGTTAA 481 GTCCCAAATT GCATCTATTG CTAATTCCAA GTGTGCTTTT ACCTATAGAT AATGAAAAAT 541 TTACACACAA GTACTCACGC ATGCACACAC ACATATACAT ACATACATAT TTTAGGGCTT 601 CTGTGTCTTT TTTACTATTC CAAAGCCATT CTGTCTGAAA AAGCTGATGT TGCCTCAGAT 661 GCAAACTAAG AAAAAGACAT AGCAACAATA ACAAGTAAGA TAGGACTTAG AAATTCCATG 721 TTCCCATTCC TGCTGTGGCC CACATGTGAT TCAAGCCCTG AGATAGGCTA TTTAGATTCA 781 GTGTGAGTAT ATCTCATGGG ATCTTCAGTT AAGCAGAGAT GGAGAGGAAA ATGGACCCAA 841 ATGAGATGAT GATTATCTTT GCCATAATAT ACAATAGGAC AGGCTGACCC CAAATTCTCT 901 GTAGGTTATG AGAACCCTTG ACCTTCACCA GGCATAGGAT TCTTTCCATC CCTGTTTCAT 961 ATTTTTTTTC CTTTCTCTCT TTCATAAGTA GAAAAATCAC ATGTTCTTTT ATGTTGCTTT 1021 AAATTTCAAA ATAATTTAAC ATTTATGTCT AAACAAGCGA AGGAAGAATA ATTTTTAAAA 1081 TATTTTCATA AAATCTTCTC CCTTACCCAA GTACTTAATC TTCTAACTCA GAAAAGCCTA 1141 ATTCCTTA //
Primer: pAxCALNL_F1 Sequence file: RDB07818_A3Gac.seq
>07818_07818_A3Ga_pAxCALNL_F1_A08_02.ab1 1 TTATTATCTT ATCATGTCTG CTCGAAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC 61 GGCGATCTTG CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA 121 CTTGCCGGTC AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT 181 GGCGGTTGTA ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC 241 GTGTTCCAGC ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC 301 GACCCCGGCG TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC 361 TACCTGGTAG CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC 421 GTCCTCGTCC CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC 481 GTTTGTAGCC TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG 541 CTCGCCGCGC TGGTTCACAC CCAGTGTCTT ACCGGTGTCC AAGTCCACCA CCTTAGCCTC 601 GAAGAAGGGC ACCACCTTGC CTACTGCGCC AGGCTTGTCG TCCCCTTCGG GGGTGATCAG 661 AATGGCGCTG GTTGTTTCTG TCAGGCCGTA GCCCTGGCGG ATGCCTGGTA GGTGGAAGCG 721 TTTGGCCACG GCCTCACCTA CCTCCTTGCT GAGCGGCGCC CCGCCGCTGG CGATCTCGTG 781 CAAGTTGCTT AGGTCGTACT TGTCGATGAG AGTGCTCTTA GCGAAGAAGC TAAATAGTGT 841 GGGCACCAGC AGGGCAGATT GAATCTTATA GTCTTGCAAG CTGCGCAAGA ATAGCTCCTC 901 CTCGAAGCGG TACATGAGCA CGACCCGAAA GCCGCAGATC AAGTAGCCCA GCGTGGTGAA 961 CATGCCGAAG CCGTGGTGAA ATGGCACCAC GCTGAGGATA GCGGGTGTCG GGGGATGATC 1021 TGGGTTGCCC GAAGATGGGG GTCGCGGGCA TGACTGAATC GGGACACAAG CGGTGCGGGT 1081 TGCGCTAAGG CCTACGCCCT TTGGGGCAAT CCGGGTAACT GGCCCCACCT TACCTGTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

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Other resources in our bank

human albumin

External Database
Human albumin

Reference sequence