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pGL4-phALB

Promoter collection, Human ALB promoter

Catalog number RDB07818
Resource name pGL4-phALB
Alternative name U2911-5
Clone info. PCR amplified human ALB promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 73402814 to 73404284(NC_000004.12); relatively -1473 to -2, where +1 corresponds to 1 nt of NM_000477.7.
Comment PCR cloning, forward primer:tcctttcaggactcccttga; reverse primer: caaacggagggaaattagca.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human albumin genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human albumin

          Reference sequence
            

          Distribution information

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          RDB07818 pGL4-phALB DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07818_A3Gap1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07818_A3Gaa.seq check
          >07818_07818_A3Ga_pGL4-4174F_G07_19.ab1
              1 ACGGGTCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
             61 TCCTTTCAGG ACTCCCTTGA GTCATTAAAA AAAATCTTCC TATCTATCTA TGTATCTATC
            121 ATCCATCTAG CTTTGATTTT TTCCTCTTCT GTGCTTTATT AGTTAATTAG TACCCATTTC
            181 TGAAGAAAAA ATAACATAAG ATTATAGAAA ATAATTTCTT TCATTGTAAG ACTGAATAGA
            241 AAAAATTTTC TTTCATTATA AGACTGAGTA GAAAAAATAA TACTTTGTTA GTCTCTGTGC
            301 CTCTATGTGC CATGAGGAAA TTTGACTACT GGTTTTGACT GACTGAGTTA TTTAATTAAG
            361 TAAAATAACT GGCTTAGTAC TAATTATTGT TCTGTAGTAT CAGAGAAAGT TGTTCTTCCT
            421 ACTGGTTGAG CTCAGTAGTT CTTCATATTC TGAGCAAAAG GGCAGAGGTA GGATAGCTTT
            481 TCTGAGGTAG AGATAAGAAC CTTGGGTAGG GAAGGAAGAT TTATGAAATA TTTAAAAAAT
            541 TATTCTTCCT TCGCTTTGTT TTTAGACATA ATGTTAAATT TATTTTGAAA TTTAAAGCAA
            601 CATAAAAGAA CATGTGATTT TTCTACTTAT TGAAAGAGAG AAAGGAAAAA AATATGAAAC
            661 AGGGATGGAA AGAATCCTAT GCCTGGTGAA GGTCAAGGGT TCTCATAACC TACAGAGAAT
            721 TTGGGGTCAG CCTGTCCTAT TGTATATTAT GGCAAAGATA ATCATCATCT CATTTGGGTC
            781 CATTTTCCTC TCCATCTCTG CTTAACTGAA GATCCCATGA GATATACTCA CACTGAATCT
            841 AAATAGCCTA TCTCAGGGCT TGAATCACAT GTGGGCCACA GCAGGAATGG GAACATGGAA
            901 TTTCTAAGTC CTATCTTACT TGTTATTGTT GCTATGTCTT TTTTCTTAGT TTGCATCTGA
            961 GGCAACATCA GCTTTTTTCA GACAGAAATG GCTTTGGAAA TAGTAAAAAA AGACACAGAA
           1021 GCCCTAAAAA TATGTATGTA TGTATATGTG TGTGTGCATG CGTGGAGTAC TTGTGTGTAA
           1081 ATTTTTCATA ATCTTATAGG TTAAAAGCAC ACTTGCAATT AGCAATAAGA ATGCCAATTT
          1141 TG
          //
          Primer: pGL4-136R
          Sequence file: RDB07818_A3Gab.seq check
          >07818_07818_A3Ga_pGL4-136R_H07_22.ab1
              1 GTTCCTAAGT TTTGGCATCT TCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
             61 GCCGCCGAGG CCAGATCTTG ATCAAACGGA GGGAAATTAG CACTAATATA CTTCTTTAAC
            121 CAATAATTGT AGATTATTAA CTAGACTCAT CTTTGTATTT CATTGGCTGC CAACCGATTA
            181 CAAAATTCAT AATATTCTTG CCAAAAAATT GTTTGACTAA ATCCCTTGTG TATATTTACC
            241 ATCTGGAACT GTTTTCTCCA CAAGACCTTG TCAATGTATT AAAGTTGTGT AAAACATCTT
            301 ATCAAATTTT ACATAAGAGT TTAAACGATT TGTACCTTTT ATTCATTACC ATGGTATTTA
            361 ACTGTAATTT CCCACTTGAA CATTTTAGTT CTTGGAGTTT GCAATTTAGT TCTCAGTGTT
            421 TAGTAATTAC TAACCAAACT AAATACTTTG CTTAGAAATA AGACATACTG AAAGAGTTAA
            481 GTCCCAAATT GCATCTATTG CTAATTCCAA GTGTGCTTTT ACCTATAGAT AATGAAAAAT
            541 TTACACACAA GTACTCACGC ATGCACACAC ACATATACAT ACATACATAT TTTAGGGCTT
            601 CTGTGTCTTT TTTACTATTC CAAAGCCATT CTGTCTGAAA AAGCTGATGT TGCCTCAGAT
            661 GCAAACTAAG AAAAAGACAT AGCAACAATA ACAAGTAAGA TAGGACTTAG AAATTCCATG
            721 TTCCCATTCC TGCTGTGGCC CACATGTGAT TCAAGCCCTG AGATAGGCTA TTTAGATTCA
            781 GTGTGAGTAT ATCTCATGGG ATCTTCAGTT AAGCAGAGAT GGAGAGGAAA ATGGACCCAA
            841 ATGAGATGAT GATTATCTTT GCCATAATAT ACAATAGGAC AGGCTGACCC CAAATTCTCT
            901 GTAGGTTATG AGAACCCTTG ACCTTCACCA GGCATAGGAT TCTTTCCATC CCTGTTTCAT
            961 ATTTTTTTTC CTTTCTCTCT TTCATAAGTA GAAAAATCAC ATGTTCTTTT ATGTTGCTTT
           1021 AAATTTCAAA ATAATTTAAC ATTTATGTCT AAACAAGCGA AGGAAGAATA ATTTTTAAAA
           1081 TATTTTCATA AAATCTTCTC CCTTACCCAA GTACTTAATC TTCTAACTCA GAAAAGCCTA
          1141 ATTCCTTA
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07818_A3Gac.seq check
          >07818_07818_A3Ga_pAxCALNL_F1_A08_02.ab1
              1 TTATTATCTT ATCATGTCTG CTCGAAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC
             61 GGCGATCTTG CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA
            121 CTTGCCGGTC AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT
            181 GGCGGTTGTA ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC
            241 GTGTTCCAGC ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC
            301 GACCCCGGCG TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC
            361 TACCTGGTAG CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC
            421 GTCCTCGTCC CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC
            481 GTTTGTAGCC TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG
            541 CTCGCCGCGC TGGTTCACAC CCAGTGTCTT ACCGGTGTCC AAGTCCACCA CCTTAGCCTC
            601 GAAGAAGGGC ACCACCTTGC CTACTGCGCC AGGCTTGTCG TCCCCTTCGG GGGTGATCAG
            661 AATGGCGCTG GTTGTTTCTG TCAGGCCGTA GCCCTGGCGG ATGCCTGGTA GGTGGAAGCG
            721 TTTGGCCACG GCCTCACCTA CCTCCTTGCT GAGCGGCGCC CCGCCGCTGG CGATCTCGTG
            781 CAAGTTGCTT AGGTCGTACT TGTCGATGAG AGTGCTCTTA GCGAAGAAGC TAAATAGTGT
            841 GGGCACCAGC AGGGCAGATT GAATCTTATA GTCTTGCAAG CTGCGCAAGA ATAGCTCCTC
            901 CTCGAAGCGG TACATGAGCA CGACCCGAAA GCCGCAGATC AAGTAGCCCA GCGTGGTGAA
            961 CATGCCGAAG CCGTGGTGAA ATGGCACCAC GCTGAGGATA GCGGGTGTCG GGGGATGATC
           1021 TGGGTTGCCC GAAGATGGGG GTCGCGGGCA TGACTGAATC GGGACACAAG CGGTGCGGGT
          1081 TGCGCTAAGG CCTACGCCCT TTGGGGCAAT CCGGGTAACT GGCCCCACCT TACCTGTG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl