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pGL4-phSLC2A1

Promoter collection, Human SLCA1 promoter

Catalog number RDB07814
Resource name pGL4-phSLC2A1
Alternative name U3288-2
Clone info. PCR amplified human SLCA1 promoter sequence was inserted into a firefly luciferase vector. Genomic DNA (42,960,401 - 42,959,101nt of NC_000001.11) corresponding to 1 to 76nt of NM_006516.2 and 1.2 kb up-stream was cloned.
Comment PCR cloning, forward primer: ttgatcagacctttgga; reverse primer: ggcattcgctgtgtga.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human solute carrier family 2 (facilitated glucose transporter), member 1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human solute carrier family 2 (facilitated glucose transporter), member 1

          Reference sequence
            

          Distribution information

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          RDB07814 pGL4-phSLC2A1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07814_A5Dnp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F
          Region: pause site, insert 5'
          Sequence file: RDB07814_A5Dna.seq check
          >07814_07814_B0J1F_pGL4ins_F_A12_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNC NNNNNNNNNN NNNGCTNNNC CCNGTGCAAG TGCAGGTGCC
             61 AGAACATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA GGATTTGATC
            121 AGACCTTTGG ACTGGGGGTC CTTCCCTTCA CTGTGTCTCA AGTAAGGCAC TGGTCTAGAG
            181 GAAATAAGGT GAAGTCAGAT GATAGCAATA CCACTACCAG TGCAGTGGCA CATGCCTGCA
            241 CTCCCAGCTA CTACGGAGGC TAAGGTGGGA GGATCACTTG AGCCCAGGAG TTTGAGACCA
            301 GCATTTATTT ATTTACTAAC AGACTAAATA AATAAACCAA CAATACCACC AACCAGTAAA
            361 TGAGAACTTC CATGCTAAAC ACTTCATGAG CTTATCTGGA AATTGCACCT CTCCTGATAG
            421 CAATGAGATT GGTGTGGGAA TTCCATTTAC TCATAAGAAG ACAGCCTCGG CCGGGCTCAG
            481 TGGCTCACGC CTGTAATCCC AGTACTTTGG GAGGCGAGGT GGGTGGATCA CGAGGTCAAG
            541 AGTTCAAGAC CAGCCTGGCC AAAATGGTGA AACCCCGTCT CTACTAAAAA TAAAAATAAT
            601 TAGCCAGGGG TGATAGCGGG CGCCTGTAAT CCCAGCTACT CGGGAGGCTG AGGCAGANAA
            661 CTGCTTGAAT CCGGGAGGCG GAGGTTACAG TGAGCCGAGA TCGTGCCGCT GCACTCCANC
            721 CTGGGCGACA GAGCGAGACT CTGTCTGANA AAAAAAAAAA TTNAANAAAA AAACGAAAAC
            781 NGCCTCNCTG GCNAGGAANC TCNCCCAGGN NCGCGGNCGG GCCAGTATAC AGTTGAGCTN
            841 GTTCNAANCC GNNGTCTATC ATGTCCTTNA TGTCCTATCA TGTCAGNNAG TTANCCTGNN
            901 TTTCNNATTT TCCNCACTTN NAGGNTCACC ATANANTCAC NNGAACTGTG AGAANTANAN
            961 GNANNNCGNN CNNNNNNAGG NGATTTNNAN NNCCNNNNNT TGANNNGGGN NTNNNNTTNN
          1021 NNNGNNNNNN NNNN
          //
          Primer: pGL4ins_R
          Region: Luc 5', insert 3'
          Sequence file: RDB07814_A5Dnb.seq check
          >07814_07814_B0J1F_pGL4ins_R_B12_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNAG ANNGGNGCTG GGCCCTTCTT AATGTTTTTG
             61 GCATCTTCNA TGGTGGCTTT ACCAACAGTA CCGGATTGCC AAGCTTGGCC GCCGAGGCCA
            121 GATCTTGATG GCATTCGCTG TGTGACTCAG GGCAAGTGTG CGCACCTCTC TGAGCCTCCG
            181 CGGCCTTCTC TAGCAAAATG GTGGAGCCGG TACCCGGCTG TAAGGCAAGC TGGGATGCGC
            241 GGAGTAGCGG GTGGAAAGCT GGACTGGAGT TCTCACTCGG CCGCTGGGTG ACTTCGGTGC
            301 ACTAGGGATA GTAACAGTAC CACCTCGTAG GGTTGTAGAA AGACCAAGTG AGTTAATATT
            361 TGTAAAGTGT TTAGAACAGC GTCTACTGCA TGGAAAGTAG TATTTATTTG TTAGAGAGAC
            421 TCCAGGAGGT CTTGCCCAGG GATTGACTTC TCCCTTCCCT GTCTCCACCC CCCACATTCC
            481 CGTTTGTGAG CCAAGCCTTG CTTTTGCACC GGCCGGGCTC TCTTGCCTGG AAATGCATAC
            541 CCATCTCAAA CCTGGGCTTT AAAATCACCT CCTCTGGGAC GCCTTCCTCT ACTTCTCACA
            601 GTTCAGGTGA GTCTATGGTG ACCTGTAAGT GTGGAAAATA TGAAAGGCAG GCTAACTGAC
            661 TGACATGATA GGACATTAAG GACATGATAG ACCTCGGGTT TGAACCAGCT CAACTGTATA
            721 CTGGCCCGGC CGCGATCCTG GGCGAGTTCC TGGCCAGTGA GGCTGTTTTC GTTTTTTTCT
            781 TTAATTTTTT TTTTTCTCAG ACAGAGTCTC GCTCTGTCGC CCAGGCTGGA NNGCAGCGGC
            841 ACGATCTCGG CTCACTGTAA CCTCCGCCTC NCGGANNCAA GCAGTTCTCT GCCTCANCCT
            901 CCNGANNANC TGGGNANTAC NGGNGCCCGC TATCNCCCCN GGCNNNNTAT TTTTATTTTT
            961 TAGNANANAN NGGGGTTTCN NNNNTTTNGG NNNNGNCTNG NNNNNNNCTN NTNGANCNNN
          1021 NNNNANNNNN CCNNCCNNCN NNNCNNNANN NNNNNNNN
          //
          Primer: pAxCALNLF1
          Region: Luc 3',
          Sequence file: RDB07814_A5Dnc.seq check
          >07814_07814_B0J1F_pAxCALNLF1_C12_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTG TCTGNTCGNN GCGGCCGGCC GCCCCGACTC TAGAATTATT
             61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT
            121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT
            181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT
            241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC
            301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG
            361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT
            421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA
            481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC
            541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG
            601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA
            661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA
            721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT
            781 CGTGCAAGTT GCTTANGTCG TACTTGTCGA TGANAGTGCT CTTANCGAAN AANCTAAATA
            841 GTGTGGGCAC CNGCANGGNA GATTGAATCT TATAGTCTTG CAAGCTGCNC AAGAATANCT
            901 NCTCCNCGAA NCGGNACATG ANCNCNANCC GAAAGCCNCA NNTCNNGTAN CCNANNNNNN
            961 NNANNNTGNC NAANCCGNGN NNAAANNNNN NNNCNCNNAN NANAGCGGNN NNNGGGNNNN
          1021 ATNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl