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pGL4-phPGR

Promoter collection, Human PGR promoter

Catalog number RDB07809
Resource name pGL4-phPGR
Alternative name U3063-9
Clone info. PCR amplified human PGR promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 101131251 to 101129781(NC_000011.10); relatively -2188 to -717, where +1 corresponds to 1 nt of NM_001202474.3; relatively -1438 to +33, where +1 corresponds to 1 nt of NM_000926.4.
Comment PCR cloning, forward primer: aatagtgctgatggttgcaca; reverse primer: aaggcttaccccgattagtga. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human progesterone receptor genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human progesterone receptor

          Reference sequence
            

          Distribution information

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          RDB07809 pGL4-phPGR DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07809_A5A6.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07809_A5A6a.seq check
          >07809_07809_A5A6_3_pGL4-4174F_E01_13.ab1
              1 GATGGGTCAG ACTTTCTCTG GCTACTGGCC GGTACCTGAG CTCGCTAGCC TCGAGGATAA
             61 TAGTGCTGAT GGTTGCACAA CTCTGAGTAC ATGAAAAATC AATGAACTGA TACTTTGAGT
            121 GAGCTGTATG ATACTGGAAT TACACCTCAA TAAAGCATGG TAACTGTTTT AAGATAGGCT
            181 GGAAAGAGAA AGCCTGAAAA CAACAATAAT GATATTAATA AATTAGTTTA CTTCTCTAGT
            241 CTCATATACT TCTGTGCCCA CACTTGCTCC TGTTCTATTC ATAATGGTCC CCTTGCAGTT
            301 GCCATATTAT ATCCTGCCAT TTGATGCCCG GTGAACATTC TATACCTGCT TCCCAGAATT
            361 CTCTTTACCT TTCCTCTATC TGCCTAACTT CCACATATCT AAAATTAATC AGAGTAAACT
            421 ATTTACTAGA ACAACCAACT CCAAATCCTA GTAACCTAAC ATGATAAAGG TTTGTTTCTC
            481 ACTCATATAG CCCCTCCCCA GATGATCGAG GGGTCCAGGC TCCTTACCTC TAGTGGCTCC
            541 CCCACCTTCT GGAGTCTTCT GCATTCTTTA TACATGGTTG AGATAAACTA TGAGTCATTA
            601 GCACAGCTAG ACCTTGAGGT CCTACAAGAA AATTTGCAAA TCATTCACTC TGTTTTGAAC
            661 AAGGTATATT TAAGATGATG TTAAAATACC CAATGGTCTT GGGTCAAATA CAGTTTATGA
            721 CTGTGTATCT AAAATATATA TTGCAATATT CTTCCCTTTT TCTACTGACT TCATGAATTT
            781 AGCGGGGATC CATTTTATAA GCTCAAAGAT AATTACTTTT CAGACTAAGA ATATTTAGGG
            841 TAAAAAGTAC TGTTCAACAT CTCTACTGAG GATGTTATGA TGTAGCACAC TGTATAAGCT
            901 GGAGCTAAAG GAAACTTTCC TTAAAGTGCT ATTTACTAAA AATTGGAACA CATTCCTTAA
            961 GACAAATCGA AGTGTGGCAC ACAACATCCA AACTTCCATC ATAGATACAG AGGTGTTACC
           1021 ATCTCCCACT CCCAAATTTC TTTGTCACGC TGAGGATACT CAAGAGGAGC AGACATGGTT
           1081 GGGTCGGCAG CAAGGAGAAC CTGCAAGGCC AATTCACTTT TTATGGAACT CAATAAAGGG
          1141 GGGAGAGAGA GAAATTCGTC T
          //
          Primer: pGL4-136R
          Sequence file: RDB07809_A5A6b.seq check
          >07809_07809_A5A6_3_pGL4-136R_F01_16.ab1
              1 GGATCTAGGT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
             61 GCCGCCGAGG CCAGATCTTG ATAAGGCTTA CCCCGATTAG TGACAGCTGT GGACTGGCCA
            121 GACAGCTTTC TAACAACGCC TCCTCCTCTA GGGAGGCCCC GCCCAAAGCC CCTCCCTACC
            181 CCAATTACCG GTAGGATCTG AAACTCTGGA GTTGGCATTT CCACCCGTTA TTCTGAATGC
            241 TACTCTCAAT AGCAGGTTCT TTGGGATGGA ACCTCATAAG CATATTACGT TTTGTTTTGC
            301 AAATTAAGAA TTATGCCCTA TCTAATTGGA AAAATGAATA GATTCTATCA GAAGTAGAAT
            361 TTTTGTCACC ATTTTAAGAT TTCAGTTTTG TAAAGATTTA ACACAGAGGA AGACATTTGG
            421 CTACATTATC TTTTAAAATA ATAAATGTAT CAAGGACGAT ACTAAATAAG AGATTCTCTC
            481 CCTTATGAGT TCCATAAAAG TGAATGCTTT CAAGTTTCTC CTGCTGCGAC CAACATGTCC
            541 TGCTCCTCTT GAGTATCCTC AGCGTGACAA AGAAATTTGG GAGTGGGAGA TGGTAACACC
            601 TCTGTATCTA TGATGGAAGT TTGGATGTTG TGTGCCACAC TTCGATTTGT CTTAAGGAAT
            661 GTGTTCCAAT TTTTAGTAAA TAGCACTTTA AGGAAAGTTT CCTTTAGCTC CAGCTTATAC
            721 AGTGTGCTAC ATCATAACAT CCTCAGTAGA GATGTTGAAC AGTACTTTTT ACCCTAAATA
            781 TTCTTAGTCT GAAAAGTAAT TATCTTTGAG CTTATAAAAT GGATCCCCGC TAAATTCATG
            841 AAGTCAGTAG AAAAAGGGAA GAATATTGCA ATATATATTT TAGATACACA GTCATAAACT
            901 GTATTTGACC CAAGACCATT GGGTATTTTA ACATCATCTT AAATATACCT TGTTCAAAAC
            961 AGAGTGAATG ATTTGCAAAT TTTCTTGTAG GACCTCAAGG TCTAGCTGTG CTAATGACTC
           1021 ATAGTTTATC TCAACCCATG TATAAGAATG CAGAAGACTC CCAGAATGTG GGGGGAGCAC
           1081 TAGAGGGTAA GGAGTCTGAC TCCTCGATCA TCTGGGGAAG GGGCCTATAT GGAATGAAAT
          1141 CCAAGCTTTC ATCCAATGGG T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl