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pGL4-phPGR (#RDB07809)

Promoter collection, Human PGR promoter

Alternative name U3063-9
Clone info. PCR amplified human PGR promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 101131251 to 101129781(NC_000011.10); relatively -2188 to -717, where +1 corresponds to 1 nt of NM_001202474.3; relatively -1438 to +33, where +1 corresponds to 1 nt of NM_000926.4.
Comment PCR cloning, forward primer: aatagtgctgatggttgcaca; reverse primer: aaggcttaccccgattagtga. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human progesterone receptor genomic DNA
Depositor|Developer DNA Bank, |
 

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RDB07809 pGL4-phPGR DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phPGR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07809).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07809_A5A6.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07809_A5A6a.seq check
>07809_07809_A5A6_3_pGL4-4174F_E01_13.ab1
    1 GATGGGTCAG ACTTTCTCTG GCTACTGGCC GGTACCTGAG CTCGCTAGCC TCGAGGATAA
   61 TAGTGCTGAT GGTTGCACAA CTCTGAGTAC ATGAAAAATC AATGAACTGA TACTTTGAGT
  121 GAGCTGTATG ATACTGGAAT TACACCTCAA TAAAGCATGG TAACTGTTTT AAGATAGGCT
  181 GGAAAGAGAA AGCCTGAAAA CAACAATAAT GATATTAATA AATTAGTTTA CTTCTCTAGT
  241 CTCATATACT TCTGTGCCCA CACTTGCTCC TGTTCTATTC ATAATGGTCC CCTTGCAGTT
  301 GCCATATTAT ATCCTGCCAT TTGATGCCCG GTGAACATTC TATACCTGCT TCCCAGAATT
  361 CTCTTTACCT TTCCTCTATC TGCCTAACTT CCACATATCT AAAATTAATC AGAGTAAACT
  421 ATTTACTAGA ACAACCAACT CCAAATCCTA GTAACCTAAC ATGATAAAGG TTTGTTTCTC
  481 ACTCATATAG CCCCTCCCCA GATGATCGAG GGGTCCAGGC TCCTTACCTC TAGTGGCTCC
  541 CCCACCTTCT GGAGTCTTCT GCATTCTTTA TACATGGTTG AGATAAACTA TGAGTCATTA
  601 GCACAGCTAG ACCTTGAGGT CCTACAAGAA AATTTGCAAA TCATTCACTC TGTTTTGAAC
  661 AAGGTATATT TAAGATGATG TTAAAATACC CAATGGTCTT GGGTCAAATA CAGTTTATGA
  721 CTGTGTATCT AAAATATATA TTGCAATATT CTTCCCTTTT TCTACTGACT TCATGAATTT
  781 AGCGGGGATC CATTTTATAA GCTCAAAGAT AATTACTTTT CAGACTAAGA ATATTTAGGG
  841 TAAAAAGTAC TGTTCAACAT CTCTACTGAG GATGTTATGA TGTAGCACAC TGTATAAGCT
  901 GGAGCTAAAG GAAACTTTCC TTAAAGTGCT ATTTACTAAA AATTGGAACA CATTCCTTAA
  961 GACAAATCGA AGTGTGGCAC ACAACATCCA AACTTCCATC ATAGATACAG AGGTGTTACC
 1021 ATCTCCCACT CCCAAATTTC TTTGTCACGC TGAGGATACT CAAGAGGAGC AGACATGGTT
 1081 GGGTCGGCAG CAAGGAGAAC CTGCAAGGCC AATTCACTTT TTATGGAACT CAATAAAGGG
1141 GGGAGAGAGA GAAATTCGTC T
//
Primer: pGL4-136R
Sequence file: RDB07809_A5A6b.seq check
>07809_07809_A5A6_3_pGL4-136R_F01_16.ab1
    1 GGATCTAGGT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
   61 GCCGCCGAGG CCAGATCTTG ATAAGGCTTA CCCCGATTAG TGACAGCTGT GGACTGGCCA
  121 GACAGCTTTC TAACAACGCC TCCTCCTCTA GGGAGGCCCC GCCCAAAGCC CCTCCCTACC
  181 CCAATTACCG GTAGGATCTG AAACTCTGGA GTTGGCATTT CCACCCGTTA TTCTGAATGC
  241 TACTCTCAAT AGCAGGTTCT TTGGGATGGA ACCTCATAAG CATATTACGT TTTGTTTTGC
  301 AAATTAAGAA TTATGCCCTA TCTAATTGGA AAAATGAATA GATTCTATCA GAAGTAGAAT
  361 TTTTGTCACC ATTTTAAGAT TTCAGTTTTG TAAAGATTTA ACACAGAGGA AGACATTTGG
  421 CTACATTATC TTTTAAAATA ATAAATGTAT CAAGGACGAT ACTAAATAAG AGATTCTCTC
  481 CCTTATGAGT TCCATAAAAG TGAATGCTTT CAAGTTTCTC CTGCTGCGAC CAACATGTCC
  541 TGCTCCTCTT GAGTATCCTC AGCGTGACAA AGAAATTTGG GAGTGGGAGA TGGTAACACC
  601 TCTGTATCTA TGATGGAAGT TTGGATGTTG TGTGCCACAC TTCGATTTGT CTTAAGGAAT
  661 GTGTTCCAAT TTTTAGTAAA TAGCACTTTA AGGAAAGTTT CCTTTAGCTC CAGCTTATAC
  721 AGTGTGCTAC ATCATAACAT CCTCAGTAGA GATGTTGAAC AGTACTTTTT ACCCTAAATA
  781 TTCTTAGTCT GAAAAGTAAT TATCTTTGAG CTTATAAAAT GGATCCCCGC TAAATTCATG
  841 AAGTCAGTAG AAAAAGGGAA GAATATTGCA ATATATATTT TAGATACACA GTCATAAACT
  901 GTATTTGACC CAAGACCATT GGGTATTTTA ACATCATCTT AAATATACCT TGTTCAAAAC
  961 AGAGTGAATG ATTTGCAAAT TTTCTTGTAG GACCTCAAGG TCTAGCTGTG CTAATGACTC
 1021 ATAGTTTATC TCAACCCATG TATAAGAATG CAGAAGACTC CCAGAATGTG GGGGGAGCAC
 1081 TAGAGGGTAA GGAGTCTGAC TCCTCGATCA TCTGGGGAAG GGGCCTATAT GGAATGAAAT
1141 CCAAGCTTTC ATCCAATGGG T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles