RDB07807 - pGL4-phCTGF

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pGL4-phCTGF (#RDB07807)

Promoter collection, Human CTGF promoter.

Alternative name	U2927-12
Clone info.	PCR amplified human CEBPA promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 33303920 to 33302423(NC_000019.10); relatively -1356 to +142, where +1 corresponds to 1 nt of NM_001285829.1.
Comment	PCR cloning, forward primer:ccatgctctgaagcatcaac; reverse primer: actggctgtctcctctcagc. Entire sequence of insert has not been analyzed.Known difference: C to G substitution at 131,952,117.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human connective tissue growth factor genomic DNA
Depositor\|Developer	DNA Bank, \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07807	pGL4-phCTGF	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phCTGF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07807).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07807_A6A8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07807_A6A8a.seq
>07807_07807_A6A8_pGL4-4174F_E02_14.ab1 1 AAGGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA 61 TCCATGCTCT GAAGCATCAA CAACTTAAAG GAATCTACTC ATTGGTAACA CCAATGTCAA 121 ATGATGCGGC ATTACAGAAT ACAAAAATGG TATGGCCCTA GAATCACTCT CTTTATATAA 181 AATGGGAACT GGAGGTATTA ACAAAAAAGC CACTAAACAA CATAGATTCC AAATGAAAGC 241 ACAGTGAATT AGTTGGGTCT TTAGTGAAGC TAAGTAGCAC TATATTAAGA AATGGAGATA 301 TGCTTAGGTT TATTGGCCAT ATTCAACATC TGCACACACC CAGGGAGAAT AAAGCCTATT 361 CTTTCCTTGT CCTGACCTCA CTCCCTCAGG CTGCATGTTC CTTGGGTAAT GAGAAGTCAC 421 AATCACTATT CATAGATGTG TGGGGAGTCA CTAAAAATAT ATTATTCACT GTCAATCTTA 481 GTTTATATCC AGATACAACA GGGTACACTG CTCTTGTAAT GGAATCAGAC TTCTTATTTT 541 AACAAGACAA ACCAAATCCA ATCCACATTT GAAGATTATA GGTTTTAATA TAAGAAAATG 601 CACTCATTTC TCAAAGACCC TAGTGAAGCT GTGTTTAAAT GCTCCTAGGT GAACCCCCTT 661 TGCATCCCAG TGTTCCCACC CTGACACCCA GAGCCCCTAC CTACCCAACA CAGAATCATT 721 TGCTCTGATA GAACAATGGA TCCCTTTTTC TGGAAACATT GATGGCCACT CGTCCCTTGT 781 CCTTGCCTAT ATAAAACTCC TACATATATT AAGAGAAAAC TAAGCAAGAG TTTTGGAAAT 841 CTGCCCCAGG AGACTGCATC CTGAGTCACA CGCGTCTTTG TTCTCTTTCT TGTCCCAAAA 901 CCGTTACCTC AAGTGACAAA TGATCAAATC TCAAATATAG AATTCAGGTT TTACAGGTAG 961 GCATCTTGAG GATTTCAAAT GGTTAAAAGC AACTCACTCC TTTTCTACTC TTTTGGAGAG 1021 TTTCAGAGCC TATAGCCTCT AAAACGCAAA TCATTGCTAC GGTTGGGGGG GGGAGAAACC 1081 TTTTCGACTT TTTAGAGTCT GCTGTTTGCC TCTCAGCTAC CTACTCCTAA ACGATGTTAT 1141 GTTCAGTGGA ACGAAACAGG CCAACCT //
Primer: pGL4-136R Sequence file: RDB07807_A6A8b.seq
>07807_07807_A6A8_pGL4-136R_F02_17.ab1 1 GGCCTAATGT TTTTGGCATC TTCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT 61 GGCCGCCGAG GCCAGATCTT GATACTGGCT GTCTCCTCTC AGCGGGGAAG AGTTGTTGTG 121 TGAGTTTGGG GCGGGCGGCC CGAGGCTTTT ATACGCTCCG GGCGGCCGCG GCTCGCCAAT 181 GAGCTGAATG GAGTCCTACA CAAACAGGGA CATTCCTCGC ATTCCTCCCC ACCTTCCTGC 241 CTCATCAACT CACACCGGAT TGATCCTGAC CCCTTGACAC TCAGCATTCC TCCGTCTGAA 301 AAAGCTGGCA CACTCCAGCT CACAGAAAAA AAAAAAAGGA AAAAAAAAAG CGCAGTATTT 361 CCAGCACCAA ATAGAATTTT TTTTTCCCTA TCCTCTTCGC ACCACTCCTG ATTCATATCA 421 TTTATAAACA CACTGGCAAT TATTTCTTAT TTCTTTTTCG AATAAGTTTG CCCTGTTCTG 481 TCCACTGACA TACATCCTTT TTAGGAAGTA GGTAGCTGAA GAGGCAAACA GCAGGAATTC 541 CTAAAAAATT CGAAAAGGTT TCTCCCCCCC AACCCTTAGC AATGATTTGC GTTTTAGAGG 601 CTATAGGCTC TTGAAACTCT CCAAAGAGTA GAAAAGGAGT GAGTTGCTTT TAACCATTTG 661 AAATCCTCAA GATGCCTACC TGTAAAACCC TGAATTCTAT ATTTGAGATT TGATCATTTG 721 TCACTTGAGG TAACGGTTTT GGGACAAGAA AGAGAACAAA GACGCGTGTG ACTCAGGATG 781 CAGTCTCCTG GGGCAGATTT CCAAAACTCT TGCTTAGTTT TCTCTTAATA TATGTAGGAG 841 TTTTATATAG GCAAGGACAA GGGACGAGTG GCCATCAATG TTTCCAGAAA AAGGGATCCA 901 TTGTTCTATC AGAGCAAATG ATTCTGTGTT GGGTAGGTAG GGGCTCTGGG TGTCAGGTGG 961 GAACACTGGG ATGCAAAGGG GGTTCACCTA GGAGCATTTA ACACAGCTTC ACTAGGTCTT 1021 TGAGAAATGA GTGCATTTTT CTTATATAAA CTATATCTCA ATGTGGATGG ATTGCATTTG 1081 TCTGTAAATA GAGTCTGATT CCATTACAAG AGCATTGTAA CCTGCTGAAT CTGCAATTAT 1141 AAACTTAAGG AT //
Primer: pAxCALNL_F1 Sequence file: RDB07807_A6A8c.seq
>07807_07807_A6A8_pAxCALNL_F1_G02_20.ab1 1 ACAAATCTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA 61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TTGCGGATCT TGCGGGCGTC 121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT 181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT 241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC 301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG 361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG 421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG 481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA 541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC 601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT 661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA 721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC 781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG 841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC 901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGT 961 GAACATGCCG AAGCCGTGGT GAAATGGCAC CACGCTGAGG ATAGCGGTGT CGGGGATGAT 1021 CTGGTTGCCG AGATGGGGTC GCGGGCATGA CTGAATCGGA CACAAGCGGT GCGGTGCGTA 1081 GCTACGCCCT GGGCAATCCG TACTGCACTA CTGATCATGA ATCAAGCGAT GGATTTTGTT 1141 CCGGATCGGA AGTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

Link

Other resources in our bank

human connective tissue growth factor

External Database
Human connective tissue growth factor

Reference sequence