RDB07725 - pGL4-phPARP1(ADPRT)

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pGL4-phPARP1(ADPRT) (#RDB07725)

Promoter collection, Human PARPI promoter

Alternative name	U3076-5
Clone info.	PCR amplified human PARPI promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 226409429 to 226407965(NC_000001.11); relatively -1336 to +129, where +1 corresponds to 1 nt of NM_001618.4.
Comment	PCR cloning, forward primer: aggattcggcctatgtctca; reverse primer: acgccacgacctagaaacac.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human poly (ADP-ribose) polymerase 1 genomic DNA
Depositor\|Developer	DNA Bank, \|

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07725	pGL4-phPARP1(ADPRT)	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phPARP1(ADPRT) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07725).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07725_A5I3p1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07725_A5I3a.seq
>07725_07725_A5I3_pGL4-4174F_A05_02.ab1 1 AAAGGGGTCA GAACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG 61 GATAGGATTC GGCCTATGTC TCACAACCTT CTTGCTTACT CAACATCCTT GTCTCTTAAG 121 CCACTAGCTT CTTCTCTATG GTTAACACTT TTTATGAGTT TTATTCATCT GCTTATTTTT 181 CTTATCCTCT ATACCAGAAT TGAATATTTT CAAATAAAGC ACACTCATGT TACAATCTTT 241 GAAATGAAAA AAAAAAATGC ATAGGATTAG AAAAGAAACC AATTTTAATA AACTATATTT 301 TGAAGTATAG TTCTATATTA AACAACAAGA TCTAGGCCAG GTGCAGTGGC TCATGCCTGT 361 AATCCCAGCA ATTTGGGAAG TCGAGGTGGG AGGATTGCTT GAGGCCAGGG GTTCAAGACC 421 AGCCTGGGCA ACATGGAGAG ATTCCCCATC TCTTTCTTTA CACACACACA CACACACACA 481 CAAAATATCT GATAGCAACA GGTGCAGTCA TTACCACAAT TTCGAGTAGT GATGAGCTTA 541 ATAATATTTC GAGTTATCAC CAACAACTGT AAAGTAACAT GAAAACGTCT GTGATGACTA 601 TTGCCCACAA AGTCACAGGT ACTGCTAATA CTCCTGGTAT TTGTAGTCAA ATTCATAATA 661 AAGGAAATGC TAGGTTTCAG TTGGTATTTT GTCCCGACGG TCTGTGGACG GCAGGTTAGA 721 ACGCCCGTCC AAGCCAGGAG GGTGGACCTA GCACTGCAGG GTCCACCTCG GGCCAATCAA 781 CTATATTCCC GAGGCGGGGG CCTGCGCTTC CCGGACCCAG CTGCCCTCAG GGGAGAGAGG 841 ACACACTTAA GAGTTTGGGG CCGGCGTGGT AGCTCATGCC CCTGATCCCA GCACTTCGGG 901 AGGCTGAGGC GTGAAGATCA CTTGTAGCAG GAGTTTGAGA CCAGTCTAGC CAACTTGGCG 961 AGACCCTGTC CCTAAAAAAA AATTTTTTTT TTAATTAGCC AGTTGTGGGT GAGCGCCCTG 1021 TAGTCCCAGC TACTCGGGAG GCTGAGGTGG GAGGATCGCT GGGCTCAGAG TTTCCAGACT 1081 GCAGTGAGAC ATGATGCCGT ACTGCACTTC AAGCGCCGGC TGAGACCTCC AGGTCCTTCC 1141 AA //
Primer: pGL4-136R Sequence file: RDB07725_A5I3b.seq
>07725_07725_A5I3_pGL4-136R_B05_05.ab1 1 GCCTTTGCTT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG 61 GCCGCCGAGG CCAGATCTTG ATACGCCACG ACCTAGAAAC ACGCTGCCGC CTCGCCGCCT 121 CGCGTGCGCT CACCCAGCCG CAGGCGCCTG AGCGGCCAGA GCCGCCACCG AACACGCCGC 181 ACCGGCCACC GCCGTTCCCT GATAGATTGC TGATGCCTGG CCGCGGGAAC GCCCACGGAA 241 CCCGCGTCCA CGGGGCGGGG CCGGCGGCGC GCGCGCCCCC TGCCGGCCGG GGGGCGGAGT 301 TTCCCGGGCG CCTGCCGGGT GGAGCTCTGC GGGCCGCTGC CCTGGGGGCC GAGGCGGGGC 361 TTGGGCTGAG CTAGTAGCTC TTTGGAGGAC CCGGCGCCAC CCCTTTGATT GTTCTGTCCC 421 AGGAAGTCTT ACTTGATTTC ACAACTAATT TTACCCCCAA CCCCTCCCCC TTTTATTTTT 481 GAGACTGAGT CTCACCGCGC TGGAGTGCAG TGCCGCCATC ATGGCTCACT GCAGTCTGGA 541 ACTCCTGAGC CCAGCGATCC TCCCACCTCA GCCTCCCGAG TAGCTGGGAC TACAGGCGCT 601 CACCACAACT GGCTAATTAA AAAAAAATTT TTTTTAGGGA CAGGGTCTCG CCAAGTTGGC 661 TAGACTGGTC TCAAACTCCT GCTACAAGTG ATCTTCACGC CTCAGCCTCC CGAAGTGCTG 721 GGATCAGGGG CATGAGCTAC CACGCCGGCC CCAAACTCTT AAGTGTGTCC TCTCTCCCCT 781 GAGGGCAGCT GGGTCCGGGA AGCGCAGGCC CCCGCCTCGG GAATATAGTT GATTGGCCCG 841 AGGTGGACCC TGCAGTGCTA GGTCCACCCT CCTGGCTTGG ACGGGCGTTC TAACCTGCCG 901 TCCACAGACC GTCGGGACAA AATACCAACT GAAACCTAGC ATTTCCTTTA TTATGAATTT 961 GACTACAAAT ACCAGGAGTA TTAGCAGTAC CTGTGACTTT GTGGGCAATA GTCATCACAG 1021 ACGTTTTCAT GTTTACTTTA CAGTTGTTGG TGATAACTCG AAAATATTAT TAAGCTCATC 1081 ACTACTCGAA AATTGTGGTT ATGGACTGGC ACTGATGCTA TCGAATTTGT GTGTGAGTAG 1141 TGGTGGATGT GATAAAGCCA AGGGAGAAA //
Primer: pAxCALNL_F1 Sequence file: RDB07725_A5I3c.seq
>07725_07725_A3I5_pAxCALNL_F1_A12_03_ABI24.ab1 1 CACCAAGGTA TCTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT 61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT 121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT 181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT 241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC 301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG 361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT 421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA 481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC 541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG 601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA 661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA 721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT 781 CGTGCAAGTT GCTTATGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA 841 GTGTGGGCAC CAGCAGGGCA GATTGAATCT TATAGTCTTG CAAGCTGCGC AAGAATAGCT 901 CCCTCCCTCG AAGCGGTACA TGAGCACGAC CCGAAAGCCG CAGATCAAGT AGCCCAGCGT 961 GGTGACATGC CCGAAGCCCG TGGTGAAATG GCCACCCACG CTGAGGATAG CCGTGTCGGG 1021 ATGATCTGCA TGGCGAGATT GGGGTCGCGG GCCATGAACT GGATTCGGAA CACCAGGCGA 1081 TGCCGATGCC GGTAAGGCCT ACGCCCTTGG GGCCAATTCC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

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Other resources in our bank

human poly (ADP-ribose) polymerase 1

External Database
Human poly (ADP-ribose) polymerase 1

Reference sequence