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pGL4-phPARP1(ADPRT)

Promoter collection, Human PARPI promoter

Catalog number RDB07725
Resource name pGL4-phPARP1(ADPRT)
Alternative name U3076-5
Clone info. PCR amplified human PARPI promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 226409429 to 226407965(NC_000001.11); relatively -1336 to +129, where +1 corresponds to 1 nt of NM_001618.4.
Comment PCR cloning, forward primer: aggattcggcctatgtctca; reverse primer: acgccacgacctagaaacac.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human poly (ADP-ribose) polymerase 1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human poly (ADP-ribose) polymerase 1

          Reference sequence
            

          Distribution information

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          RDB07725 pGL4-phPARP1(ADPRT) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07725_A5I3p1_2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07725_A5I3a.seq check
          >07725_07725_A5I3_pGL4-4174F_A05_02.ab1
              1 AAAGGGGTCA GAACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG
             61 GATAGGATTC GGCCTATGTC TCACAACCTT CTTGCTTACT CAACATCCTT GTCTCTTAAG
            121 CCACTAGCTT CTTCTCTATG GTTAACACTT TTTATGAGTT TTATTCATCT GCTTATTTTT
            181 CTTATCCTCT ATACCAGAAT TGAATATTTT CAAATAAAGC ACACTCATGT TACAATCTTT
            241 GAAATGAAAA AAAAAAATGC ATAGGATTAG AAAAGAAACC AATTTTAATA AACTATATTT
            301 TGAAGTATAG TTCTATATTA AACAACAAGA TCTAGGCCAG GTGCAGTGGC TCATGCCTGT
            361 AATCCCAGCA ATTTGGGAAG TCGAGGTGGG AGGATTGCTT GAGGCCAGGG GTTCAAGACC
            421 AGCCTGGGCA ACATGGAGAG ATTCCCCATC TCTTTCTTTA CACACACACA CACACACACA
            481 CAAAATATCT GATAGCAACA GGTGCAGTCA TTACCACAAT TTCGAGTAGT GATGAGCTTA
            541 ATAATATTTC GAGTTATCAC CAACAACTGT AAAGTAACAT GAAAACGTCT GTGATGACTA
            601 TTGCCCACAA AGTCACAGGT ACTGCTAATA CTCCTGGTAT TTGTAGTCAA ATTCATAATA
            661 AAGGAAATGC TAGGTTTCAG TTGGTATTTT GTCCCGACGG TCTGTGGACG GCAGGTTAGA
            721 ACGCCCGTCC AAGCCAGGAG GGTGGACCTA GCACTGCAGG GTCCACCTCG GGCCAATCAA
            781 CTATATTCCC GAGGCGGGGG CCTGCGCTTC CCGGACCCAG CTGCCCTCAG GGGAGAGAGG
            841 ACACACTTAA GAGTTTGGGG CCGGCGTGGT AGCTCATGCC CCTGATCCCA GCACTTCGGG
            901 AGGCTGAGGC GTGAAGATCA CTTGTAGCAG GAGTTTGAGA CCAGTCTAGC CAACTTGGCG
            961 AGACCCTGTC CCTAAAAAAA AATTTTTTTT TTAATTAGCC AGTTGTGGGT GAGCGCCCTG
           1021 TAGTCCCAGC TACTCGGGAG GCTGAGGTGG GAGGATCGCT GGGCTCAGAG TTTCCAGACT
           1081 GCAGTGAGAC ATGATGCCGT ACTGCACTTC AAGCGCCGGC TGAGACCTCC AGGTCCTTCC
          1141 AA
          //
          Primer: pGL4-136R
          Sequence file: RDB07725_A5I3b.seq check
          >07725_07725_A5I3_pGL4-136R_B05_05.ab1
              1 GCCTTTGCTT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
             61 GCCGCCGAGG CCAGATCTTG ATACGCCACG ACCTAGAAAC ACGCTGCCGC CTCGCCGCCT
            121 CGCGTGCGCT CACCCAGCCG CAGGCGCCTG AGCGGCCAGA GCCGCCACCG AACACGCCGC
            181 ACCGGCCACC GCCGTTCCCT GATAGATTGC TGATGCCTGG CCGCGGGAAC GCCCACGGAA
            241 CCCGCGTCCA CGGGGCGGGG CCGGCGGCGC GCGCGCCCCC TGCCGGCCGG GGGGCGGAGT
            301 TTCCCGGGCG CCTGCCGGGT GGAGCTCTGC GGGCCGCTGC CCTGGGGGCC GAGGCGGGGC
            361 TTGGGCTGAG CTAGTAGCTC TTTGGAGGAC CCGGCGCCAC CCCTTTGATT GTTCTGTCCC
            421 AGGAAGTCTT ACTTGATTTC ACAACTAATT TTACCCCCAA CCCCTCCCCC TTTTATTTTT
            481 GAGACTGAGT CTCACCGCGC TGGAGTGCAG TGCCGCCATC ATGGCTCACT GCAGTCTGGA
            541 ACTCCTGAGC CCAGCGATCC TCCCACCTCA GCCTCCCGAG TAGCTGGGAC TACAGGCGCT
            601 CACCACAACT GGCTAATTAA AAAAAAATTT TTTTTAGGGA CAGGGTCTCG CCAAGTTGGC
            661 TAGACTGGTC TCAAACTCCT GCTACAAGTG ATCTTCACGC CTCAGCCTCC CGAAGTGCTG
            721 GGATCAGGGG CATGAGCTAC CACGCCGGCC CCAAACTCTT AAGTGTGTCC TCTCTCCCCT
            781 GAGGGCAGCT GGGTCCGGGA AGCGCAGGCC CCCGCCTCGG GAATATAGTT GATTGGCCCG
            841 AGGTGGACCC TGCAGTGCTA GGTCCACCCT CCTGGCTTGG ACGGGCGTTC TAACCTGCCG
            901 TCCACAGACC GTCGGGACAA AATACCAACT GAAACCTAGC ATTTCCTTTA TTATGAATTT
            961 GACTACAAAT ACCAGGAGTA TTAGCAGTAC CTGTGACTTT GTGGGCAATA GTCATCACAG
           1021 ACGTTTTCAT GTTTACTTTA CAGTTGTTGG TGATAACTCG AAAATATTAT TAAGCTCATC
           1081 ACTACTCGAA AATTGTGGTT ATGGACTGGC ACTGATGCTA TCGAATTTGT GTGTGAGTAG
          1141 TGGTGGATGT GATAAAGCCA AGGGAGAAA
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07725_A5I3c.seq check
          >07725_07725_A3I5_pAxCALNL_F1_A12_03_ABI24.ab1
              1 CACCAAGGTA TCTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT
             61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT
            121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT
            181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT
            241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC
            301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG
            361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT
            421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA
            481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC
            541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG
            601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA
            661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA
            721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT
            781 CGTGCAAGTT GCTTATGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA
            841 GTGTGGGCAC CAGCAGGGCA GATTGAATCT TATAGTCTTG CAAGCTGCGC AAGAATAGCT
            901 CCCTCCCTCG AAGCGGTACA TGAGCACGAC CCGAAAGCCG CAGATCAAGT AGCCCAGCGT
            961 GGTGACATGC CCGAAGCCCG TGGTGAAATG GCCACCCACG CTGAGGATAG CCGTGTCGGG
           1021 ATGATCTGCA TGGCGAGATT GGGGTCGCGG GCCATGAACT GGATTCGGAA CACCAGGCGA
          1081 TGCCGATGCC GGTAAGGCCT ACGCCCTTGG GGCCAATTCC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl