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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pME-kpmK697A-HA (#RDB07699)

Expression vector of human LATS2 kinase

Clone info. Expression vector of human LATS2 kinase.
Vector backbone pME18S (Plasmid)
Selectable markers Amp^r
Gene/insert name Human KPM (LATS2) cDNA
Depositor|Developer Hori, Toshiyuki |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Hori, T., Oncogene, 19, 3101-3109 (2000))
提供案内 (日本国内) [open/close]

MTAに書く使用条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　利用者は、研究成果の公表にあたって譲渡者の指定する文献を引用する（Hori, T., Oncogene, 19, 3101-3109 (2000))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07699 pME-kpmK697A-HA DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pME-kpmK697A-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07699).

Reference section:

Hori, T., Takaori-Kondo, A., Kamikubo, Y., Uchiyama, T., Molecular cloning of a novel human protein kinase, kpm, that is homologous to warts/lats, a Drosophila tumor suppressor. Oncogene, 19, 3101-3109 (2000). PMID 10871863. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12309_A3K5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SRapro_F
Sequence file: RDB12309_A3K5a.seq check
>07699_12309_A3K5_SRapro_F_G11_20.ab1
    1 ACGTACTCTG CTCACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA
   61 AGCTCTGAAA AACCAGAAAG TTAACTGGTA AGTTTAGTCT TTTTGTCTTT TATTTCAGGT
  121 CCCGGATCCG GTGGTGGTGC AAATCAAAGA ACTGCTCCTC AGTGGATGTT GCCTTTACTT
  181 CTAGGCCTGT ACGGAAGTGT TACTTCTGCT CTAAAAGCTG CGGAATTCCG GAATTTCCTC
  241 GAGATCCATT GTGCTGGAAA GGCCCCGGGA AGATGGAGCA GTCGCCGTCC ACGCCACCGC
  301 CGCCGCCCGG GGCTCCCCCG TCCCTGCGGG GCCAGCAGCA GCTCCAGCCA CCAGTGCCCG
  361 GTCTCCCGGC GCGAGAGGCC CGGGAGCCGC CGGCCAGGAC GCCCCCGAGG GTGTAGACCG
  421 CGCCCCTGGA GAGAGTGATA ATCTTCAAAA TGAAGACTTT GGAAAATTTT AGGTTCTCTA
  481 TAGGAACTAC AAAAATGGAA GGAAAGAACA TTTTCAAAAG GAAATTATTT TGAAAGTATG
  541 TTTACAACAA ACTGATACTA TTGACAGTTT TTTTTTTTAA ATAATAAAAC ACTTTAAGAA
  601 GATTGTATTT ATGGTAAAAG GAAACTGGAC TAACAATGAG GCCAAAGACT TTTCCTGCCA
  661 CGACTTATTC TGGAAATAGC CGGCAGCGAC TGCAAGAGAT TCGTGAGGGG TTAAAACAGC
  721 CATCCAAGTC TTCGGTTCAG GGGCTACCCG CAGGACCAAA CAGTGACACT TCCCTGGATG
  781 CCAAAGTCCT GGGGAGCAAA GATGCCACCA GGCAGCAGCA GCAGATGAGA GCCACCCCAA
  841 AGTTCGGACC TTATCAGAAA GCCTTGAGGG AAATCAGATA TTCCTTGTTG CCTTTTGCTA
  901 ATGAATCGGG CACCTCTGCA GCTGCAGAAG TGAACCGGCA AATGCTGCAG GAACTGGTGA
  961 ACGCAGGATG CGACCAGGAG ATGGCTGGCC GAGCTCTCAA GCAGACTGGC AGCAGGAGCA
 1021 TCGAGGCCGC CCTGGTAGTA CATCAGCAGA TGGGCCTACT GCACCCCGAA GTAATGAGCA
1081 GGATGTTGCC GGATCAATTA AGTAGACTCT CCCCCAAG
//
Primer: ME-1290Rv
Sequence file: RDB12309_A3K5b.seq check
>07699_12309_A3K5_ME-1290Rv_H11_23.ab1
    1 ATGCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TCTCTAGATA
   61 CTTAGCATGC AAGTTTATCG ATCTGAGCAG CGTAATCTGG AACGTCATAT GGATAGGATC
  121 CTGCATAGTC CGGGACGTCA TAGGGATAGC CCGCATAGTC AGGAACATCG TATGGGTAAA
  181 AACTAGTCAC GTACACAGGC TGGCAGCCTT CAGTCTGATC CACCAGATCA GAGCTTTCTA
  241 AATCTGAGCT CTCAGCCTGT GAAGCTTCTG CTCCTGAAGG CTTTGGGCAT CGAAAGGGGT
  301 AGCCATTGTC ATCAAAGAAC CTTCGGAAGG TGAATTCGTA AAATGCGTGC TCAGGATGCT
  361 TGTTATTGGG CGAGGTGAGT GTGTCCCAGG CCTTGGTGCT ACCTTCGCTG GCATCGTTCC
  421 AAGGGCTTTC TTCATCTACG GGGTCGAAAT TCGAGGTGTC CATGGGGTGG CTGATGGTGG
  481 GAACGTAGGG GGCTGGCTGC TTCCGGATGT CACTGGAGAA GTCAATGGCG CTGAAGAAGG
  541 GGTGGGCCTT CAGGTCATCG GCCCCATTCC GCCCCAGGCG GTGGTCTGCG GAGCAGCACA
  601 GCTTGGTGAT GAGGTCCCTG GCCTCAGGGC TCAGCTTCAC CTGGGCTGGA ATGTGGAGCG
  661 TGTTCTCCCA GTTGATCACC TTCAGCTGGG TTTCTGTGGG AGTAGGTGCC AAAAAGGGCG
  721 GCTGCCCCAC CAGCATCTCG AAGAGAATCA CTCCAACACT CCACCAGTCA CAGAGTTGAG
  781 TGTACCCTTT GCGGAGGAGC ACCTCGGGTG CGATGTAGTT TGGAGTCCCC ACCAGTGAAT
  841 GTGCCAGGCA CCTCTGGTGC TGCTTCCGCG CCCTCTGCTC TAGGGTCTTC AGCCTGTCCC
  901 CACACCGACA GTTAGACACA TCATCCCAGA GGTCGCTGGG CTCCATGCTG TCCTGTCTGA
  961 CATGGCTCCC TTTCTGGTAA TATTTGGGAA TTGTGAGTCC ACCTGAACCC AGTGCAGAGG
 1021 CCCGAAATCT GTGAGTTTAA TGTGACCCAT CCCAGATCTA TCAAAATGTA TCAGCTGATG
 1081 TCTCGGGTGA TGAAGCCAAT CTTGTGACAC TCCTCATTGA CCAAAGTTCA GCCTCCTTGC
1141 CGGA
//
Primer: Lats2#11
Sequence file: RDB12309_A3K5c.seq check
>07699_12309_A3K5_Lats2#11_H10_22.ab1
    1 TAAAGGTTAC CGGAAGCTGC AGCTGGAGCA GAATGGCCAA AGCTGGACTC TGTGAAGCTG
   61 AGCAGGAGCA GATGCGGAAG ATCCTCTACC AGAAAGAGTC TAATTACAAC AGGTTAAAGA
  121 GGGCCAAGAT GGACAAGTCT ATGTTTGTCA AGATCAAAAC CCTGGGGATC GGTGCCTTTG
  181 GAGAAGTGTG CCTTGCTTGT AAGGTGGACA CTCACGCCCT GTACGCCATG GCGACCCTAA
  241 GGAAAAAGGA TGTCCTGAAC CGGAATCAGG TGGCCCACGT CAAGGCCGAG AGGGACATCC
  301 TGGCCGAGGC AGACAATGAG TGGGTGGTCA AACTCTACTA CTCCTTCCAA GACAAAGACA
  361 GCCTGTACTT TGTGATGGAC TACATCCCTG GTGGGGACAT GATGAGCCTG CTGATCCGGA
  421 TGGAGGTCTT CCCTGAGCAC CTGGCCCGGT TCTACATCGC AGAGCTGACT TTGGCCATTG
  481 AGAGTGTCCA CAAGATGGGC TTCATCCACC GAGACATCAA GCCTGATAAC ATTTTGATAG
  541 ATCTGGATGG TCACATTAAA CTCACAGATT TCGGCCTCTG CACTGGGTTC AGGTGGACTC
  601 ACAATTCCAA ATATTACCAG AAAGGGAGCC ATGTCAGACA GGACAGCATG GAGCCCAGCG
  661 ACCTCTGGGA TGATGTGTCT AACTGTCGGT GTGGGGACAG GCTGAAGACC CTAGAGCAGA
  721 GGGCGCGGAA GCAGCACCAG AGGTGCCTGG CACATTCACT GGTGGGGACT CCAAACTACA
  781 TCGCACCCGA GGTGCTCCTC CGCAAAGGGT ACACTCAACT CTGTGACTGG TGGAGTGTTG
  841 GAGTGATTCT CTTCGAGATG CTGGTGGGGC AGCCGCCCTT TTTGGCACCT ACTCCCACAG
  901 AAACCCAGCT GAAGGTGATC AACTGGGAGA ACACGCTCCA CATTCCAGCC CAGGTGAAGC
  961 TGAGCCCTGA GGCCAGGGAC CTCATCACCA AGCTGTGCTG CTCCGCAGAC CACCGCCTGG
 1021 GGGCGGGAAT GGGGGCCCGA TGACCCTGAA GCCACCCCTT TCTTCAGCGT CATTGACTCT
 1081 CCAGTGACAT CTGCAGCAGC AGCCCCTACG GTCCCACAAT CAGCCAACCC CCAATGGAAA
1141 CAAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hori, T., Molecular cloning of a novel human protein kinase, kpm, that is homologous to warts/lats, a Drosophila tumor suppressor. Oncogene, 19, 3101-3109 (2000). PMID 10871863. [link to RRC of NBRP]