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pME-kpmK697A-HA

Expression vector of human LATS2 kinase

Catalog number RDB07699
Resource name pME-kpmK697A-HA
Clone info. Expression vector of human LATS2 kinase.
Vector backbone pME18S (Plasmid)
Selectable markers Amp^r
Gene/insert name Human KPM (LATS2) cDNA
Depositor|Developer Hori, Toshiyuki |
Other clones in our bank

External Database
Human KPM (LATS2)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Hori, T., Oncogene, 19, 3101-3109 (2000))
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 利用者は、研究成果の公表にあたって譲渡者の指定する文献を引用する(Hori, T., Oncogene, 19, 3101-3109 (2000))。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB07699 pME-kpmK697A-HA DNA solution Please contact us.

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hori, T., Molecular cloning of a novel human protein kinase, kpm, that is homologous to warts/lats, a Drosophila tumor suppressor. Oncogene, 19, 3101-3109 (2000). PMID 10871863.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12309_A3K5p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SRapro_F
          Sequence file: RDB12309_A3K5a.seq check
          >07699_12309_A3K5_SRapro_F_G11_20.ab1
              1 ACGTACTCTG CTCACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA
             61 AGCTCTGAAA AACCAGAAAG TTAACTGGTA AGTTTAGTCT TTTTGTCTTT TATTTCAGGT
            121 CCCGGATCCG GTGGTGGTGC AAATCAAAGA ACTGCTCCTC AGTGGATGTT GCCTTTACTT
            181 CTAGGCCTGT ACGGAAGTGT TACTTCTGCT CTAAAAGCTG CGGAATTCCG GAATTTCCTC
            241 GAGATCCATT GTGCTGGAAA GGCCCCGGGA AGATGGAGCA GTCGCCGTCC ACGCCACCGC
            301 CGCCGCCCGG GGCTCCCCCG TCCCTGCGGG GCCAGCAGCA GCTCCAGCCA CCAGTGCCCG
            361 GTCTCCCGGC GCGAGAGGCC CGGGAGCCGC CGGCCAGGAC GCCCCCGAGG GTGTAGACCG
            421 CGCCCCTGGA GAGAGTGATA ATCTTCAAAA TGAAGACTTT GGAAAATTTT AGGTTCTCTA
            481 TAGGAACTAC AAAAATGGAA GGAAAGAACA TTTTCAAAAG GAAATTATTT TGAAAGTATG
            541 TTTACAACAA ACTGATACTA TTGACAGTTT TTTTTTTTAA ATAATAAAAC ACTTTAAGAA
            601 GATTGTATTT ATGGTAAAAG GAAACTGGAC TAACAATGAG GCCAAAGACT TTTCCTGCCA
            661 CGACTTATTC TGGAAATAGC CGGCAGCGAC TGCAAGAGAT TCGTGAGGGG TTAAAACAGC
            721 CATCCAAGTC TTCGGTTCAG GGGCTACCCG CAGGACCAAA CAGTGACACT TCCCTGGATG
            781 CCAAAGTCCT GGGGAGCAAA GATGCCACCA GGCAGCAGCA GCAGATGAGA GCCACCCCAA
            841 AGTTCGGACC TTATCAGAAA GCCTTGAGGG AAATCAGATA TTCCTTGTTG CCTTTTGCTA
            901 ATGAATCGGG CACCTCTGCA GCTGCAGAAG TGAACCGGCA AATGCTGCAG GAACTGGTGA
            961 ACGCAGGATG CGACCAGGAG ATGGCTGGCC GAGCTCTCAA GCAGACTGGC AGCAGGAGCA
           1021 TCGAGGCCGC CCTGGTAGTA CATCAGCAGA TGGGCCTACT GCACCCCGAA GTAATGAGCA
          1081 GGATGTTGCC GGATCAATTA AGTAGACTCT CCCCCAAG
          //
          Primer: ME-1290Rv
          Sequence file: RDB12309_A3K5b.seq check
          >07699_12309_A3K5_ME-1290Rv_H11_23.ab1
              1 ATGCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TCTCTAGATA
             61 CTTAGCATGC AAGTTTATCG ATCTGAGCAG CGTAATCTGG AACGTCATAT GGATAGGATC
            121 CTGCATAGTC CGGGACGTCA TAGGGATAGC CCGCATAGTC AGGAACATCG TATGGGTAAA
            181 AACTAGTCAC GTACACAGGC TGGCAGCCTT CAGTCTGATC CACCAGATCA GAGCTTTCTA
            241 AATCTGAGCT CTCAGCCTGT GAAGCTTCTG CTCCTGAAGG CTTTGGGCAT CGAAAGGGGT
            301 AGCCATTGTC ATCAAAGAAC CTTCGGAAGG TGAATTCGTA AAATGCGTGC TCAGGATGCT
            361 TGTTATTGGG CGAGGTGAGT GTGTCCCAGG CCTTGGTGCT ACCTTCGCTG GCATCGTTCC
            421 AAGGGCTTTC TTCATCTACG GGGTCGAAAT TCGAGGTGTC CATGGGGTGG CTGATGGTGG
            481 GAACGTAGGG GGCTGGCTGC TTCCGGATGT CACTGGAGAA GTCAATGGCG CTGAAGAAGG
            541 GGTGGGCCTT CAGGTCATCG GCCCCATTCC GCCCCAGGCG GTGGTCTGCG GAGCAGCACA
            601 GCTTGGTGAT GAGGTCCCTG GCCTCAGGGC TCAGCTTCAC CTGGGCTGGA ATGTGGAGCG
            661 TGTTCTCCCA GTTGATCACC TTCAGCTGGG TTTCTGTGGG AGTAGGTGCC AAAAAGGGCG
            721 GCTGCCCCAC CAGCATCTCG AAGAGAATCA CTCCAACACT CCACCAGTCA CAGAGTTGAG
            781 TGTACCCTTT GCGGAGGAGC ACCTCGGGTG CGATGTAGTT TGGAGTCCCC ACCAGTGAAT
            841 GTGCCAGGCA CCTCTGGTGC TGCTTCCGCG CCCTCTGCTC TAGGGTCTTC AGCCTGTCCC
            901 CACACCGACA GTTAGACACA TCATCCCAGA GGTCGCTGGG CTCCATGCTG TCCTGTCTGA
            961 CATGGCTCCC TTTCTGGTAA TATTTGGGAA TTGTGAGTCC ACCTGAACCC AGTGCAGAGG
           1021 CCCGAAATCT GTGAGTTTAA TGTGACCCAT CCCAGATCTA TCAAAATGTA TCAGCTGATG
           1081 TCTCGGGTGA TGAAGCCAAT CTTGTGACAC TCCTCATTGA CCAAAGTTCA GCCTCCTTGC
          1141 CGGA
          //
          Primer: Lats2#11
          Sequence file: RDB12309_A3K5c.seq check
          >07699_12309_A3K5_Lats2#11_H10_22.ab1
              1 TAAAGGTTAC CGGAAGCTGC AGCTGGAGCA GAATGGCCAA AGCTGGACTC TGTGAAGCTG
             61 AGCAGGAGCA GATGCGGAAG ATCCTCTACC AGAAAGAGTC TAATTACAAC AGGTTAAAGA
            121 GGGCCAAGAT GGACAAGTCT ATGTTTGTCA AGATCAAAAC CCTGGGGATC GGTGCCTTTG
            181 GAGAAGTGTG CCTTGCTTGT AAGGTGGACA CTCACGCCCT GTACGCCATG GCGACCCTAA
            241 GGAAAAAGGA TGTCCTGAAC CGGAATCAGG TGGCCCACGT CAAGGCCGAG AGGGACATCC
            301 TGGCCGAGGC AGACAATGAG TGGGTGGTCA AACTCTACTA CTCCTTCCAA GACAAAGACA
            361 GCCTGTACTT TGTGATGGAC TACATCCCTG GTGGGGACAT GATGAGCCTG CTGATCCGGA
            421 TGGAGGTCTT CCCTGAGCAC CTGGCCCGGT TCTACATCGC AGAGCTGACT TTGGCCATTG
            481 AGAGTGTCCA CAAGATGGGC TTCATCCACC GAGACATCAA GCCTGATAAC ATTTTGATAG
            541 ATCTGGATGG TCACATTAAA CTCACAGATT TCGGCCTCTG CACTGGGTTC AGGTGGACTC
            601 ACAATTCCAA ATATTACCAG AAAGGGAGCC ATGTCAGACA GGACAGCATG GAGCCCAGCG
            661 ACCTCTGGGA TGATGTGTCT AACTGTCGGT GTGGGGACAG GCTGAAGACC CTAGAGCAGA
            721 GGGCGCGGAA GCAGCACCAG AGGTGCCTGG CACATTCACT GGTGGGGACT CCAAACTACA
            781 TCGCACCCGA GGTGCTCCTC CGCAAAGGGT ACACTCAACT CTGTGACTGG TGGAGTGTTG
            841 GAGTGATTCT CTTCGAGATG CTGGTGGGGC AGCCGCCCTT TTTGGCACCT ACTCCCACAG
            901 AAACCCAGCT GAAGGTGATC AACTGGGAGA ACACGCTCCA CATTCCAGCC CAGGTGAAGC
            961 TGAGCCCTGA GGCCAGGGAC CTCATCACCA AGCTGTGCTG CTCCGCAGAC CACCGCCTGG
           1021 GGGCGGGAAT GGGGGCCCGA TGACCCTGAA GCCACCCCTT TCTTCAGCGT CATTGACTCT
           1081 CCAGTGACAT CTGCAGCAGC AGCCCCTACG GTCCCACAAT CAGCCAACCC CCAATGGAAA
          1141 CAAA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hori, T., Molecular cloning of a novel human protein kinase, kpm, that is homologous to warts/lats, a Drosophila tumor suppressor. Oncogene, 19, 3101-3109 (2000). PMID 10871863.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl