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pGL4-phHIF1A (#RDB07693)

Promoter collection, Human HIF1A promoter

Alternative name U3136-6
Clone info. PCR amplified human HIF1A promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 61694082 to 61695550(NC_000014.9); relatively -1431 to +38, where +1 corresponds to 1 nt of NM_001530.4.
Comment PCR cloning, forward primer: cccatggttcttttcaagaca; reverse primer: atcctcctgtcccctcagac. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07693.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07693 pGL4-phHIF1A DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phHIF1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07693).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07693_A4La.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07693_A4Laa.seq check
>07693_07693_A4La_pGL4-4174F_A04_01.ab1
    1 ATGGGTCAGA CATTTCTCTG GCCTACTGGC CGGTACCTGA GCTCGCTAGC CTCGAGGATC
   61 CCATGGTTCT TTTCAAGACA TAATACATAT CATTTAGTGT TGTAGATCTG AAAAAACAAA
  121 AGTAGCGTGA AGATCAAAAA TTTTCTAAAG AGACGGAGTC TCGCTACGTT CCCTAGGCTG
  181 GAACACCCAG GCTTCTCCAG CCTCACACCT CTGAGTAGCT GGAACCACCC TGTCCGCTAA
  241 GGTCAATGTT TAATCGTATC TTTGTAGGTC TACTGACCAG TTAAAAAGAG GTGCTGTATA
  301 CATTGGTTGT TGTCTTGTCA GAGTTTGATG CTTCTATATA GACCATTGTT TTTACATGCT
  361 AATACAATTG AAAGCCACTA CAGATATTTA TATTTACAAC CCAAAGCTAG GTTTTAACAA
  421 GAAACTCATA AGGCAAAGGT GAGAAGTAAA ATAATTTAGC GCCAAGTGGA GATATATGTG
  481 CAATGCTACT TTGTTGGGCT CAAAACATAT TTTTCTTTTA GAAGACTGAC AGGCTTGAAG
  541 TTTATGCCTC CAAAGACAAA AGTGATTACG TTTTGTTTAG TAGCTTGCAA AGTTGCCAAA
  601 GGCCATTTTT TCTACTCTTT CCCTGAAATT GGTTTATATG CTTATTAAAG TCATTTATAC
  661 CTATTTGCAA ATGCTTAACA TAGTTTCAGA TTTTAAGATT TCCCTGCAAC TTTATTTCCC
  721 TTGAAGTTTA CAGCAACAGG AGTTCATTTT TATTTTTAAT TGCATTTATT CAGTAAGTAA
  781 ACTCCGCCAC AGAAAAACTT AGTAGACAAG GTGAGTTCCC CTGTGCTCCG TGGCAAAGAG
  841 TGCGGTGGGT GACATTGACC CATGGTTAGG TAATCTGGTA AGGAAGACCC CGTTGTAACA
  901 CATCTGAGCA ACGAGACCAA AGGAAGGGCT TGCTGCCACG AGGCGAAGTC TGCTTTTTTT
  961 GAACAGAGAG CCCAGCAGAG TTGGGCGGCA ATCGTGCCCA GCACTGAGGC CGAGGAGAAG
 1021 AGAGCAGGAG CATTACATTA CTGCACCAAG AGTAGGAAAA TATGATGCAT GTTTGGGACC
 1081 AGGCAACCGA AATCCCTTCT CAGCAGCGGC TTCCTAAAGG TGGCAACGAC GTTCCTTCGG
1141 AGAAGGCGCA GAAGTTCCCC CAGAGAAACG TCG
//
Primer: pGL4-136R
Sequence file: RDB07693_A4Lab.seq check
>07693_07693_A4La_pGL4-136R_B04_04.ab1
    1 GACTATGTTT GGCATCTTCC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC
   61 CGCCGAGGCC AGATCTTGAT ATCCTCCTGT CCCCTCAGAC GAGGCAGCAC TGTGCACTGA
  121 GGAGCTGAGG CAGCGTCAGG GGGCGGGCAA GGGCGGAGGC GCGCTCGGGC GCGCGGGGAG
  181 GGGAGAGGGG GCGGGAGCGC GCCCGCTCGC CTGCCCTGGC CGGCGCGCGC TCCCGCAAGC
  241 CCGCCTCACC TGAGGTGGAG GCGGGTTCCT CGAGATCCAA TGGCGAGCGA CGCAGGCGGC
  301 AAGTCCCCAC CCCAGCCCAA GCCGCTCTCA GCCAATCAGG AGGCGGTCAG CTGAGGCACA
  361 GCTGGGACGG GCTGCGACGC TCACGTGCTC GTCTGTGTTT AGCGGCGGAG GAAAGAGAAA
  421 GGAGATGGGG GTGCGGCTCA GCCCGAGTCT GGGGACTCTG CGCCTTCTCC GAAGGAAGGC
  481 GGTGCCCGGC TTTGGGAGGC GCTGCTGAGA AGGGATTTCG GTTGCCTGGT CCCAAACATG
  541 CATCATATTT TCCTACTCTT GGTGCAGTAA TGTAATGCAC CTGCTCTCTT TCTCCTCGGC
  601 CTCAGTGCTG GGCACGATTG CCGCCCAACT CTGCTGGGCT CTCTGTTCAA AAAAGCAGAC
  661 TTCGCCTCGT GGCAGCAAGC CCTTCCTTTG GTCTCGTTGC TCAGATGTGT TACAACGGGG
  721 TCTTTCCTTA CCAGATTACC TAACCATGGG TCAATGTCAC CCACCGCACT CTTTGCCACG
  781 GAGCACAGGG GAACTCACCT TGTCTACTAA GTTTTTCTGT GGCGGAGTTT ACTTACTGAA
  841 TAAATGCAAT TAAAAATAAA AATGAACTCC TGTTGCTGTA AACTTCAAGG GAAATAAAGT
  901 TGCAGGGAAA TCTTAAAATC TGAAACTATG TTAAGCATTT GCAAATAGGT ATAAATGACT
  961 TTAATAAGCA TATAAACCCA ATTTCAGGGA AAGAGTAGAA AAAATGGCCC TTTGGCAACT
 1021 TTGCCAGCTA CTAAACAAAA CGTAATCACT TTTGTCTTGA GGCATAACTT CAAGCCTGTC
 1081 AGGTCTTCTA GAGGAAAAAT ATGTTTTGAG CCACCAAAGG TAGCATTGCA CATATATCTT
1141 CCACCTTGCG CTAAATACTT ACTTCGTCAC GCTTTGGACC TTTATTTGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Chiba, R., ALK signaling cascade confers multiple advantages to glioblastoma cells through neovascularization and cell proliferation. PLoS One 12 (8): e0183516 (2017). PMID 28837676. [link to RRC of NBRP]