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pGL4-phAPP

Promoter collection, Human APP promoter

Catalog number RDB07692
Resource name pGL4-phAPP
Alternative name U3080-14
Clone info. PCR amplified human APP promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 26172063 to 26170592(NC_000021.9); relatively -1293 to +179, where +1 corresponds to 1 nt of NM_000484.4.
Comment PCR cloning, forward primer: cctagtgcgggaaaagacac; reverse primer: agcaggagcagtgccaaac. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human amyloid beta (A4) precursor protein genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human amyloid beta (A4) precursor protein

          Reference sequence
            

          Distribution information

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          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07692 pGL4-phAPP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07692.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07692_A4Lm.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07692_A4Lma.seq check
          >07692_07692_A4Lm_pGL4-4174F_A08_02.ab1
              1 GCTGGGTCAG ACATTTCTCT GGCCTACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
             61 CCTAGTGCGG GAAAAGACAC ACAAGACAAC ATTTAGGGGA GCTGGTACAG AAATGACTTC
            121 CCAGGAAGGA AGTCTGTACC CCGCTGGCTG AGCCATCCTT CCCGGGCCTA GGCACCCTTG
            181 TCAGCGCAAT GAGCAAGGGA GAGAAGGCAG GCTGCAGTGC AGCCCTCAGA AGGGCCAGAG
            241 CACTCCCTGG CTTCAGTCCT TCGCTCCAAG CCCTGTGTGG AGTGGGCTGT GGCTTGGTAA
            301 CTAAATGCTA CTTCAGGTCA AGAGCAGGGG ATACATCTGG GCAGTTCTAG AGCATTCTAA
            361 ACTATCTGGA CACTAACTGG ACAGTGGACG GTTTGTGTTT AATCCAGGAG AAAGTGGCAT
            421 GGCAGAAGGT TCATTTCTAT AATTCAGGAC AGACACAATG AAGAACAAGG GCAGCGTTTG
            481 AGGTCAGAAG TCCTCATTTA CGGGGGTCGA ATACGAATGA TCTCTCCTAA TTTTTCCTTC
            541 TTCCCCAACT CAGATGGATG TTACATCCCT GCTTAACAAC AAAAAAAGAC CCCCCGCCCC
            601 GCAAAATCCA CACTGACCAC CCCCTTTAAC AAAACAAAAC CAAAAACAAA CAAAAATATA
            661 AGAAAGAAAC AAAACCCAAG CCCAGAACCC TGCTTTCAAG AAGAAGTAAA TGGGTTGGCC
            721 GCTTCTTTGC CAGGTCCTGC GCCTTGCTCC TTTGGTTCGT TCTAAAGATA GAAATTCCAG
            781 GTTGCTCGTG CCTGCTTTTG ACGTTGGGGG TTAAAAAATG AGGTTTTGCT GTCTCAACAA
            841 GCAAAGAAAA TCCTATTTCC TTTAAGCTTC ACTCGTTCTC ATTCTCTTCC AGAAACGCCT
            901 GCCCCACCTC TCCAAACCGA GAGAAAAAAC GAAATGCGGA TAAAAACGCA CCCTAGCAGC
            961 AGTCCTTTAT ACGACACCCC CGGGAGGGCC TGCGGGGTCG GATGATTCAA GCTCACGGGG
           1021 ACGAGCAGGA GCGCTCTCGA CTTTTTCTAG AGCCTCAGCG TCCCTAGGAC TCACCTTTCC
           1081 CTGATCCCTG CACCGTCCCT CTCCTGACCC AGACTCCTCC CCTCCCACTG TTCACGAGCT
          1141 CAGGTGGGCC GTCGGCTCGG GGGGAAGCCG GAAGGGGGGC GCCCGCATTG TG
          //
          Primer: pGL4-136R
          Sequence file: RDB07692_A4Lmb.seq check
          >07692_07692_A4Lm_pGL4-136R_B08_05.ab1
              1 ACTTTTTGAT TTGGCATCTT CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTTGG
             61 CCGCCGAGGC CAGATCTTGA TAGCAGGAGC AGTGCCAAAC CGGGCAGCAT CGCGACCCTG
            121 CGCGGGGCAC CGAGTGCGCT GCTGTGCGAG TGGGATCCGC CGCGTCCTTG CTCTGCCCGC
            181 GCCGCCACCG CCGCCGTCTC CCGGGGCCCC CGCGCACGCT CCTCCGCGTG CTCTCGCCTA
            241 CCGCTGCCGA GGAAACTGAC GGAGCCCGAG CGCGGCGGCG GGGCTCAGAG CCAGGCGAGT
            301 CAGCTGATCC GGCCCACCCC GCTCGGCACC CGAGAGAGAC CCCTAGCGGC GCCGCCGGGG
            361 AACTGCGCCC GCTCGCGCCG GGAGGGGCCC TCGCGCCCCG CGCCCACAGG TGCAGCGCCC
            421 TTGGCGCCGC CTGCACCCCA CGCGCCCCCT CCGCTCCCCG GCCGACGGCC CACCTGGGCT
            481 TCGTGAACAG TGGGAGGGAG AGTCTGGGGC CAGGAGAGGG ACGGTGCAGG ATCAGGGAAA
            541 GGTGAGTCCT AGGACGCTGA GGCTCTAGAA AAGTCGAGAG CGCTCCTGCT CGTCCCCGTG
            601 AGCTTGAATC ATCCGACCCC GCAGGCCTCC CGGGGGTGTC GTATAAAGGA CTGCTGCTAG
            661 GGTGCGTTTT TATCCGCATT TCGTTTTTTC TCTCGGTTTG GAGAGGTGGG GCAGGCGTTT
            721 CTGGAAGAGA ATGAGAACGA GTGAAGCTTA AAGGAAATAG GATTTTCTTT GCTTGTTGAG
            781 ACAGCAAAAC CTCATTTTTT AACCCCCAAC GTCAAAAGCA GGCACGAGCA ACCTGGAATT
            841 TCTATCTTTA GAACGAACCA AAGGAGCAAG GCGCAGGACC TGGCAAAGAA GCGGCCAACC
            901 CATTTACTTC TTCTTGAAAG CAGGGTTCTG GGCTTGGGTT TTGTTTCTTT CTTATATTTT
            961 TGTTTGTTTT TGGTTTTGTT TTGTTAAAGG GGGTGGTCAG TGTGGATTTT GCGGGGCGGG
           1021 GGGGTCTTTT TTTTGTGTTA AGCAGGGATG TACATCATCT GAGTTGGGGG AAGAAGGAAA
           1081 AATAGAGAGA TCATTTCGTA TTTCGAACCC CCGGTAAATG AAGACTTCCT GGACCTCAAA
          1141 CGGCTTACCC TTGAATTCCT TTTCCAAT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl