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pGL4-phSOD1 (#RDB07686)

Promoter collection, Human SOD1 promoter

Alternative name U2950-3
Clone info. PCR amplified human SOD1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 31658211 to 31659681(NC_000021.9); relatively -1411 to +60, where +1 corresponds to 1 nt of NM_000454.4.
Comment PCR cloning, forward primer: gaaatctgccgaacttcctg; reverse primer: ccgtctccgcgactacttta. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human superoxide dismutase 1, soluble genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07686.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07686 pGL4-phSOD1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phSOD1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07686).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07686_A4Lm.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07686_A4Lma.seq check
>07686_07686_A4Lm_pGL4-4174F_G07_19.ab1
    1 GGTGGGTCAG ACATTTCTCT GGCCTACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
   61 GAAATCTGCC GAACTTCCTG GAACCCAAAG AAACTTTAGC CTTGGGCAAA GGCCCTTTGG
  121 CCAGCATTTG CACTGTTTAT GCAACCGTTT AGAATATACG AATTATCTGG AGACTACTAC
  181 CAAATACAAC AGGCAAAACT GCAAATATGT ATACTTCCTA GAGGATGATA AAAAAATGTG
  241 AATTGTATTT CTCTGATAGA GGATGCATTA GAGTCTGAGG GTCTAAATAG CGTAAATAAT
  301 AAATAAGTAA ATAAATCGAT AGTAGTGTAC TCCAAACGAG GCTGGAATAG CTTCTATTGT
  361 TGTTTCACAC TGGACTTCAA TTAAGTCTCA GTATTTTGCC ATACTCAATA TTAAGTACTA
  421 GGCTGGACGT GGTGGCTCAT GTCTGTAATC CCAGCACTTT GGGAGGCCGA GGTGGGTAGA
  481 TGGCTGGCTT GAGCTCAGGA GTTTGAAACC AGCCTGGGCA ACATGGTAAA ACCCCATCTG
  541 TACCCAAAAT ACAAAAATCA GCCAGGTGTG GTGGCACATG CCTGTGGTCC CAGGTACTTG
  601 GGAGGCTGAG GCAGGAGGAT GGCTTGAACC CAGGAGGTGG AGGCTGCAGT GAGCTATGAT
  661 GGCGCCACTG CACTCCAGCC TGGGTGACAG AGCGAGACCC TGTCTCAAAA ATCAAACAAA
  721 CAACCCCCTC GCCCCGGACA AAAGTAGTTT GCACTATTTT CTCATTTCAC AATATGTTTT
  781 TGAAATATTT CCCTTGAAAG GTAAGTCATA TTTATCATTC CTGTTGTATG GAGGCATCAT
  841 AAATTATTTC ACCATTCTAC CCTCCTTGAG TGTTGTGGCC TTTAGCCAGA CAAAACGCAG
  901 TGATGCCTAG AGCCAACTAG TGCCGTTTGT ATCTGTAGGT GTGCCTGCCA ACAGAAAATA
  961 TAAAGATACG ATCTGCACAA TTAGAAATCT ATACTAGACT AGAAATGCAG GGAAGAAAGT
 1021 TAGTCCGGGA TGAGGTGTAA CGACTTTCTA TACCCTCGAA ACTAAAACCA GACCAAATTG
 1081 AAACTACAAG GCACTCACTG GGCGTCCAAT GTCTGATACA TGAAGCCTGG CAACTCAGGC
1141 TTAC
//
Primer: pGL4-136R
Sequence file: RDB07686_A4Lmb.seq check
>07686_07686_A4Lm_pGL4-136R_H07_22.ab1
    1 GCTTTCTAGT TTATGGCATC TTCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT
   61 GGCCGCCGAG GCCAGATCTT GATCCGTCTC CGCGACTACT TTATAGGCCA GACCTCCGCG
  121 CCTCGCCCAC TCTGGCCCCA AACCAATCGC CTCGCACGCG CTCAGCCCGC CCCCTGCGGC
  181 AGCCTCGGGT CGCGAGTGGC CGGGAATGAC TGGGGGCGGG GCCAAGGGGC CGCGTGCGCC
  241 GGAGACTCCC CCCGCCCCGC AGCCGGGAAA CGTCCAGAAC CCCGAAATGT GGAGAAAACA
  301 ACCTTCTTTT CACGGGGGCG TGGCCCGCCG GTTGCAGTAC GCGAAATTGG CAAAATGATG
  361 TAAACAGGGT ACGCTTTGAA AATCTGAAGT CACTTTGTCT AGAAATGTCA TTAGTTATGA
  421 CTGAGTTTGG CCACAGCGTC TCATTTGTTA CTCAGCAATT GGGACGCCCC AAGATGGATG
  481 CTTTTGTAGT TTTCATTTTT TTGTCTTGTT TTTTAGTTTT CTGAGGGTAT AGAAAGTCGC
  541 TACACCTCAA TCCCGGGACT TACCTTTTCT TCCCCTGCAA TTTTCTTAGT CCTTAGTATA
  601 GATTTTCTTA TTTGGTTGGC AGAATTCGGT ATTCTTTTTA TATTTTTCCT GTTTGGCAAG
  661 GCCACAACCC TACAGATAAC CAAACGGCAA CTAGTTGGCT TCTAGGCATC ACCTGCGTTT
  721 TTGTCTGGCC TAAAGGCCAC AACACTCAAG GAGGGTAGAA TGGTGAAATA ATTTATGATG
  781 CCTCCATACA ACAGGAATGA TAAATATGAC TTACCTTTCA AGGGAAATAT TTCAAAAACA
  841 TATTGTGAAA TGAGAAAATA GTGCAAACTA CTTTTGTCCG GGGCGAGGGG GTTGTTTGTT
  901 TGATTTTTGA GACAGGGTCT CGCTCTGTCA CCCAGGCTGG AGTGCAGTGG CGCCATCATA
  961 GCTCACTGCA GCCTCCACCT CCTGGGTTCA AGCCATCCTC CTGCCTCAGC CTCCCAAGTA
 1021 CCTGGGACCA CAGGCATGTG CCACCACACC TGGCTGATTT TGTATTTTGG GTACAGATGG
 1081 GGGTTTTACC ATGATGACCA GCTGCTTCAA CTCTTGAGCT CAAGGTCAGC ATCTACCAAC
1141 TCGGACTTCC GAAATGCTGG TATACGTACA TTGAAGCGAT CTTAAACGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles