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pGL4-phCD44

Promoter collection, Human CD44 promoter

Catalog number RDB07685
Resource name pGL4-phCD44
Alternative name U2916-2
Clone info. PCR amplified human CD44 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 35137703 to 35139162(NC_000011.10); relatively -1167 to +293, where +1 corresponds to 1 nt of NM_000610.3.
Comment PCR cloning, forward primer: ctcagaagtcctggcatggt; reverse primer: ggaagttggctgcagttttt. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human CD44 molecule (Indian blood group) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human CD44 molecule (Indian blood group)

          Reference sequence
            

          Distribution information

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          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07685 pGL4-phCD44 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07685.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07685_A4D2p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07685_A4D2a_2.seq check
          >07685_07685_A4D2_pGL4-4174F_E11_14.ab1
              1 ATCGTGGGTA GGAACATTGG CTCTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG
             61 AGGATCTCAG AAGTCCTGGC ATGGTTCCTT TCATCTTGCC ACAGCCACTG ATAATCACTT
            121 TCATTTTCTG TGTAACTCAC CAGGCAAGAA GTCCATGCAG ATTTACTTTT AGTAGTTCAC
            181 ATGACAAATA AATACTGCGT TTGATTTCCA AACATTAAAC CATAGTATAT TATAGATAGA
            241 TATAGAGTTA TCATTCAAAG TATGATATTT CAATCTCAAA AGGCTTCCCC TGAAGAATAT
            301 TACAAACTCT TCCTCTCTTT AAGATCTGCT GGGTAGGAAA GATGGGAGAA AATGAATTAA
            361 TGTTTACACA GAAAGGAGGA TAATGGGGGC AAAAATAATA GATGAACGTA TGGGTGGATG
            421 AGAGAATGGA TAAAATGATA GGTGGATATG TTGATCTTGG ACAGATGGGA AATGAGTGGA
            481 TATATCAATA AACAGATATG TGGGTGGATG GGTGGAGAAG AGGATGGTGG ATGGTTGTGG
            541 TTTTATGAAG AGATGTGAAA AAGGAAGTGT GGAATGATGG ATGAGAAGTT GTATGGGAAG
            601 ATGAATAGAA GAATAGGTGG TTGAATAAAT TACAAGGTGT GTGGTTGGAT GAATGAATGA
          661 GTGGGATGAT AGATGGACCT
          //
          Primer: pGL4-136R
          Sequence file: RDB07685_A4D2b_2.seq check
          >07685_07685_A4D2_pGL4-136R_F11_17.ab1
              1 GCTCCTAATG TTTTTGGCAT CCTTCCATGG TGGCTTTACC AACAGTACCG CGATTGCCAA
             61 GCTTGGCCGC CGAGGCCAGA TCTTGATGGA AGTTGGCTGC AGTTTTTATT CGAGGTTGAA
            121 AACAGTGACC TAAGACGGAG GGAGGGAGCG AGCGAAGGAC ACACCCAAGC AAGGGGGGCT
            181 GTGACTAAGC AGCCCGCAGA GGTTTTAAGA AGTAGCAGCC CTCCCGGGCG GGCCCCTCTG
            241 CCCCCGCCCC GTGCTCTGCT GAGGCTGTAA ATAATCGGGG CTGCCAGGGA CGTGGGTCGG
            301 GGAACCTGGA GTGTCGCGGG GTGCCTGTGC CTCCGAGCCC CACTGGCCCC TGCGCCCAGA
            361 GACGCACTGG CTTTCTTCTC CCGCTGGCCC CGCGGCGTCA GGACAGAGGA TGACCGAACC
            421 GTAAAACCTT GCACCATTGG GTTCAGCCTT TGGCCTCTCC TTTCCCGCTA AATTTCTCTG
            481 AATCCGGCTG GAGCAAGATC TCTGGGTTTC ACAGGATGTT GGATATCCTG GGAGAGGAGC
            541 TGGAGAGAGG GCGAGGTCTT GCCCTGGGTG GCGCAGTGCC TCCGTGCCCA CAGTGGGGAG
            601 TGAGGGGTGG GGAGTTGGTG AATCTTCCAA GTGGAAAGAG GGAGAGCTCA TTCTTTTTCC
            661 CCTCAATAAC AAGTCCACAT GGCTTGAATG AATTTTAGCT TCTTGGCAGA ACAGCTCAGG
            721 GAAAGCTACT GATGGCCTGT CATAGGGCTG GCATTTGGCT CAGCTAACCC ATGTGTTCAA
            781 GCCAGTGTGA ATGGGCACCT CTCTCCACAC ACATGCTCAT TCATCCTCCT AAATATCCTT
            841 CCATCCGCCC ATCCATCTAT CCACCCATCT TTCCTTATGT CCTTCTGGGG CTCTCACATC
            901 CATCCATCCT CCTGTCCATC CACTAACCAC TTAGGTCCAT CTATCATCCC ACTCATTCAT
            961 TCATCCAACC ACACACCTTT TTAATTTTAT TCAAACCACC CTATTCTTCT AATTCATCTT
           1021 CCCATACAAA CTTTCTCATC CCAATCAATT CCCACACTTC CCTTTTTTCA CATCTCTTTC
           1081 AATAAAAACC AACACCCATC CACATTCCTC TTCTCACCCA TCACCCAACA ATATCCTGGT
          1141 TTATTGGAAA TATATTCGAC TCAGTTGCCA TCTGGTCCAC AAGGAG
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07685_A4D2c_2.seq check
          >07685_07685_A4D2_pAxCALNL-F1_E01_05_ABI08.ab1
              1 TACGGTATAT CTTAATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT
             61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT
            121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT
            181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT
            241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC
            301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG
            361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT
            421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA
            481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC
            541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG
            601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA
            661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA
            721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT
            781 CGTGCAAGTT GCTTAGGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA
            841 GTGTGGGGCA CCAGCAGGGC AGATTGAATC TTATAGTCTT GCAAGCTGCG CAAGAATAGC
            901 TCCTCCTCGA AGCGGTACAT GAGCACGACC CGAAAGCCGC AGATCAAGTA GCCCAGCGTG
            961 GTGAACATGC CGAAGCCGTG ATGAATGGCA CCACGCTGAG GATAGCGGTG TTCGGGATGA
           1021 TCTGGGTTGC CGGAAGATGG GGTCGCGGCC ATGAACTGGA TCGGACACAG CGGATGCGTT
          1081 GCAGCTAAGG CCTACGCCCT TGGGGCCAAT T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl