Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phCD44 (#RDB07685)

Promoter collection, Human CD44 promoter

Alternative name U2916-2
Clone info. PCR amplified human CD44 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 35137703 to 35139162(NC_000011.10); relatively -1167 to +293, where +1 corresponds to 1 nt of NM_000610.3.
Comment PCR cloning, forward primer: ctcagaagtcctggcatggt; reverse primer: ggaagttggctgcagttttt. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human CD44 molecule (Indian blood group) genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07685.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 謝辞の表明を必要とする。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07685 pGL4-phCD44 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phCD44 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07685).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07685_A4D2p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07685_A4D2a_2.seq check
>07685_07685_A4D2_pGL4-4174F_E11_14.ab1
    1 ATCGTGGGTA GGAACATTGG CTCTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG
   61 AGGATCTCAG AAGTCCTGGC ATGGTTCCTT TCATCTTGCC ACAGCCACTG ATAATCACTT
  121 TCATTTTCTG TGTAACTCAC CAGGCAAGAA GTCCATGCAG ATTTACTTTT AGTAGTTCAC
  181 ATGACAAATA AATACTGCGT TTGATTTCCA AACATTAAAC CATAGTATAT TATAGATAGA
  241 TATAGAGTTA TCATTCAAAG TATGATATTT CAATCTCAAA AGGCTTCCCC TGAAGAATAT
  301 TACAAACTCT TCCTCTCTTT AAGATCTGCT GGGTAGGAAA GATGGGAGAA AATGAATTAA
  361 TGTTTACACA GAAAGGAGGA TAATGGGGGC AAAAATAATA GATGAACGTA TGGGTGGATG
  421 AGAGAATGGA TAAAATGATA GGTGGATATG TTGATCTTGG ACAGATGGGA AATGAGTGGA
  481 TATATCAATA AACAGATATG TGGGTGGATG GGTGGAGAAG AGGATGGTGG ATGGTTGTGG
  541 TTTTATGAAG AGATGTGAAA AAGGAAGTGT GGAATGATGG ATGAGAAGTT GTATGGGAAG
  601 ATGAATAGAA GAATAGGTGG TTGAATAAAT TACAAGGTGT GTGGTTGGAT GAATGAATGA
661 GTGGGATGAT AGATGGACCT
//
Primer: pGL4-136R
Sequence file: RDB07685_A4D2b_2.seq check
>07685_07685_A4D2_pGL4-136R_F11_17.ab1
    1 GCTCCTAATG TTTTTGGCAT CCTTCCATGG TGGCTTTACC AACAGTACCG CGATTGCCAA
   61 GCTTGGCCGC CGAGGCCAGA TCTTGATGGA AGTTGGCTGC AGTTTTTATT CGAGGTTGAA
  121 AACAGTGACC TAAGACGGAG GGAGGGAGCG AGCGAAGGAC ACACCCAAGC AAGGGGGGCT
  181 GTGACTAAGC AGCCCGCAGA GGTTTTAAGA AGTAGCAGCC CTCCCGGGCG GGCCCCTCTG
  241 CCCCCGCCCC GTGCTCTGCT GAGGCTGTAA ATAATCGGGG CTGCCAGGGA CGTGGGTCGG
  301 GGAACCTGGA GTGTCGCGGG GTGCCTGTGC CTCCGAGCCC CACTGGCCCC TGCGCCCAGA
  361 GACGCACTGG CTTTCTTCTC CCGCTGGCCC CGCGGCGTCA GGACAGAGGA TGACCGAACC
  421 GTAAAACCTT GCACCATTGG GTTCAGCCTT TGGCCTCTCC TTTCCCGCTA AATTTCTCTG
  481 AATCCGGCTG GAGCAAGATC TCTGGGTTTC ACAGGATGTT GGATATCCTG GGAGAGGAGC
  541 TGGAGAGAGG GCGAGGTCTT GCCCTGGGTG GCGCAGTGCC TCCGTGCCCA CAGTGGGGAG
  601 TGAGGGGTGG GGAGTTGGTG AATCTTCCAA GTGGAAAGAG GGAGAGCTCA TTCTTTTTCC
  661 CCTCAATAAC AAGTCCACAT GGCTTGAATG AATTTTAGCT TCTTGGCAGA ACAGCTCAGG
  721 GAAAGCTACT GATGGCCTGT CATAGGGCTG GCATTTGGCT CAGCTAACCC ATGTGTTCAA
  781 GCCAGTGTGA ATGGGCACCT CTCTCCACAC ACATGCTCAT TCATCCTCCT AAATATCCTT
  841 CCATCCGCCC ATCCATCTAT CCACCCATCT TTCCTTATGT CCTTCTGGGG CTCTCACATC
  901 CATCCATCCT CCTGTCCATC CACTAACCAC TTAGGTCCAT CTATCATCCC ACTCATTCAT
  961 TCATCCAACC ACACACCTTT TTAATTTTAT TCAAACCACC CTATTCTTCT AATTCATCTT
 1021 CCCATACAAA CTTTCTCATC CCAATCAATT CCCACACTTC CCTTTTTTCA CATCTCTTTC
 1081 AATAAAAACC AACACCCATC CACATTCCTC TTCTCACCCA TCACCCAACA ATATCCTGGT
1141 TTATTGGAAA TATATTCGAC TCAGTTGCCA TCTGGTCCAC AAGGAG
//
Primer: pAxCALNL_F1
Sequence file: RDB07685_A4D2c_2.seq check
>07685_07685_A4D2_pAxCALNL-F1_E01_05_ABI08.ab1
    1 TACGGTATAT CTTAATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT
   61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT
  121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT
  181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT
  241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC
  301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG
  361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT
  421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA
  481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC
  541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG
  601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA
  661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA
  721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT
  781 CGTGCAAGTT GCTTAGGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA
  841 GTGTGGGGCA CCAGCAGGGC AGATTGAATC TTATAGTCTT GCAAGCTGCG CAAGAATAGC
  901 TCCTCCTCGA AGCGGTACAT GAGCACGACC CGAAAGCCGC AGATCAAGTA GCCCAGCGTG
  961 GTGAACATGC CGAAGCCGTG ATGAATGGCA CCACGCTGAG GATAGCGGTG TTCGGGATGA
 1021 TCTGGGTTGC CGGAAGATGG GGTCGCGGCC ATGAACTGGA TCGGACACAG CGGATGCGTT
1081 GCAGCTAAGG CCTACGCCCT TGGGGCCAAT T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles