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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phBCL2

Promoter collection, Human BCL2 promoter

Catalog number RDB07567
Resource name pGL4-phBCL2
Alternative name U2644(2022)-4
Clone info. PCR amplified human BCL2 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 63320698 to 63319352(NC_000018.10); relatively -1318 to +29, where +1 corresponds to 1 nt of NM_000633.2.
Comment PCR cloning, forward primer: actcacacggccagaaagg; reverse primer: tcgattcccagacttctgct.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human B-cell CLL/lymphoma 2 genomic DNA
Depositor|Developer DNA Bank, | Kishikawa, Shotaro |
Other clones in our bank

External Database
Human B-cell CLL/lymphoma 2

          Reference sequence
            

          Distribution information

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          RDB07567 pGL4-phBCL2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07567.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07567_A5A6.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07567_A5A6a.seq check
          >07567_07567_A5A6_pGL4-4174F_C01_07.ab1
              1 GCTGGGGGTC AGACATTTCT CTGGCCTACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
             61 ATACTCACAC GGCCAGAAAG GGTCCAGGCG GTTCCCGGCG CTTTTCCAGC CCTTGTTTTC
            121 ATGGCGCACC CTCCCGCCAG CCGCCCCCCT CCGCACTCCG TCGTCCGCCC GGCCCGGCCG
            181 CGTGCGGTTC CCCGGGAGCC CCCACCCCGT CGCGGACCCC AGCGACCACC AAGTCCGCAC
            241 GCGGCCTGCC GCAGGCCTGA GCAGAAGGCC CCGCGCACAC CCACCGCGCC GCGGCCGCGC
            301 GGGAGGCCTG TGCCGCCCGC GCCACCCACT GGCCGGGCCC CGCGGGCGCA GCGGAGCGGG
            361 CGGGTGGCCG GCCCGGACGC GCCCTCCCCG GCCGCGGCCC CGCGCGCCAT GTGCCCCCGG
            421 CGGGACGCGC CACTCCCGGG CCTGCCGCGG CGCCTTTAAC CCGGGCCAGG GAGCGGGGCG
            481 GAGGGGGCGG TCGGGTGGCT CAGAGGAGGG CTCTTTCTTT CTTCTTTTTT TGAATGAACC
            541 GTGTGACGTT ACGCACAGGA AACCGGTCGG GCTGTGCAGA GAATGAAGTA AGAGGACAGG
            601 CACCACAGCC CCGCTCCCGC CCCCTTCCTC CCGCGCCCGC CCCTCCGCGC CGCCTGCCCG
            661 CCCGCCCGCC GCGCTCCCGC CCGCCGCTCT CCGTGGCCCC GCCGCGCTGC CGCCGCCGCC
            721 GCTGCCAGCG AAGGTGCCGG GGCTCCGGGC CCTCCCTGCC GGCGGCCGTC AGCGCTCGGA
            781 GCGGGCTGCG CGGCGGGAGC TCCGGGAGGC GGCCGTAGCC AGCGCCGCCG CGCAGGACCA
            841 GGAGGAGGAG AAAGGGTGCG CAGCCCGGAG GCGGGGGTGC GCCGGTGGGG GTGCAGCGGA
            901 AGAGGGGGGT CCAGGGGGGG GAGAACTTCC GTAGCAGTCA TCCCTTTTTA GGGAAAAGAG
            961 GGGAAATGTT AAAACCCTCC CCCAACCACC TTCCTTTCTT CCCCACCCCT TCGCCGGCAC
           1021 CAACACACAG CCGCGGGGCC TTCCTAGCCG CTTCGGAGCA CCGGGCCGGG GCTCAGGGCC
           1081 GGCCGGTTCC TGGCCCTTTC CAATTTATTC CCAGGCCAGG CCGTTTTTTC CGGGAAAATG
           1141 GCCATTGGCC TTGGTTTCCG GAAAGGTTTT AAATCTCAGG AAAGCAGGGG GCATTCCCCC
          1201 TGGGGCCCTC TCTCAGT
          //
          Primer: pGL4-136R
          Sequence file: RDB07567_A5A6b.seq check
          >07567_07567_A5A6_pGL4-136R_D01_10.ab1
              1 GCGGCCTTTA AGTTTTTTGG CATCTTGCAT GGTGGCTTTA CCAACAGTAC CGGATTGCCA
             61 AGCTTGGCCG CCGAGGCCAG ATCTTGATTC GATTCCCAGA CTTCTGCTTC ACAGAAATGT
            121 CAATCCGCAG GAATCCCAAC CGGAGATCTC AAGAGCTCGA GAAAAAAAAA AGGCAGCGGC
            181 GGCGGCAGAT GAATTACAAT TTTCAGTCCG GTATTCGCAG AAGTCCTGTG ATGTTTTCCC
            241 CTTCTCGGCA ATTTACACGC GCGCACACAC GCGCGGGCAC AGGCATGAAT CTCTATCCAC
            301 GGGACCGCTT CACGCCTCCC CAGGAGAGAG ACAGGGGAGA GGGGACGATG AAGGAGCCGG
            361 GGACGGAGGC AGGAATCCTC TTCTGATTAA ACTCCGAACA GCAAATGCAT TTTCCGAAAA
            421 GCTGCTGGAT AAATGAAGGC AGGACGCGCC TGGCCCGCCG GTGCCGAGCG CTAGAAGCCC
            481 GCGCTGTGTG TGGTGCGGCG AGGGGTGGGG AGAAGGAGGT GGTGGGGGAG GGTTTTATTT
            541 TTTCCCTCTT TTCCTAAAAA GGATGACTGC TACGAAGTTC TCCCCCCTGG ACCCCCTCTT
            601 CCGCTGCACC CCACCGGCGC ACCCCGCCTC CGGGCTGCGC ACCCTTTCTC CTCCTCCCTG
            661 GTCCTGCGCG GCGGCGCTGG CTACGGCCGC CTCCCGGAGC TCCCGCCGCG CAGCCCGCTC
          721 CGAGCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl