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pGL4-phAPOE

Promoter collection, Human APOE promoter

Catalog number RDB07556
Resource name pGL4-phAPOE
Alternative name U2839-30
Clone info. PCR amplified human APOE promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 44904368 to 44905816(NC_000019.10); relatively -1428 to +21, where +1 corresponds to 1 nt of NM_001302688.2.
Comment PCR cloning, forward primer: CCTTGCCAGCCAAAGCAAAC; reverse primer: ACCTCCGCTGGGGCTGAGTA. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human apolipoprotein E genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human apolipoprotein E

          Reference sequence
            

          Distribution information

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          RDB07556 pGL4-phAPOE DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07556.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07556_A7Eop1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07556_A7Eoa.seq check
          >07556_07556_A7Eo_pGL4-4174F_A01_01_ABI08.ab1
              1 AAAGGGTCAA ACTTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
             61 CCTTGCCAGC CAAAGCAAAC AGGCCCGGCC CGGCACTGGG GGTTCCTTCT CGAACCAGGA
            121 GTTCAGCCTC CCCTGACCCG CAGAATCTTC TGATCCCACC CGCTCCAGGA GCCAGGAATG
            181 AGTCCCAGTC TCTCCCAGTT CTCACTGTGT GGTTTTGCCA TTCGTCTTGC TGCTGAACCA
            241 CGGGTTTCTC CTCTGAAACA TCTGGGATTT ATAACAGGGC TTAGGAAAGT GACAGCGTCT
            301 GAGCGTTCAC TGTGGCCTGT CCATTGCTAG CCCTAACATA GGACCGCTGT GTGCCAGGGC
            361 TGTCCTCCAT GCTCAATACA CGTTAGCTTG TCACCAAACA TACCCGTGCC GCTGCTTTCC
            421 CAGTCTGATG AGCAAAGGAA CTTGATGCTC AGAGAGGACA AGTCATTTGC CCAAGGTCAC
            481 ACAGCTGGCA ACTGGCAGAG CCAGGATTCA CGCCCTGGCA ATTTGACTCC AGAATCCTAA
            541 CCTTAACCCA GAAGCACGGC TTCAAGCCCC TGGAAACCAC AATACCTGTG GCAGCCAGGG
            601 GGAGGTGCTG GAATCTCATT TCACATGTGG GGAGGGGGCT CCCCTGTGCT CAAGGTCACA
            661 ACCAAAGAGG AAGCTGTGAT TAAAACCCAG GTCCCATTTG CAAAGCCTCG ACTTTTAGCA
            721 GGTGCATCAT ACTGTTCCCA CCCCTCCCAT CCCACTTCTG TCCAGCCGCC TAGCCCCACT
            781 TTCTTTTTTT TCTTTTTTTG AGACAGTCTC CCTCTTGCTG AGGCTGGAGT GCAGTGGCGA
            841 GATCTCGGCT CACTGTAACC TCCGCCTCCC GGGTTCAAGC GATTCTCCTG CCTCAGCCTC
            901 CCAAGTAGCT AGGATTACAG GCGCCCGCCA CCCACGCCTG GCTAAACTTT TGTATTTTTT
            961 AGTAGAGATG GGGGTTTCAC CATGTTTGGC CCAGGCTGGT CTCAAACTCC TGACCCTTTA
           1021 AGTGATTTCG CCCACTGTTG CCTCCCAAAG TGCTGGGATA CAGCGTGAGC TACGTCCCCA
          1081 GGCCCTCCAA TTCCCCACTT CCTTGTTCAA GGACTGT
          //
          Primer: pGL4-136R
          Sequence file: RDB07556_A7Eob.seq check
          >07556_07556_A7Eo_pGL4-136R_B01_02_ABI08.ab1
              1 GCTCTTATTG GTTTTTGGCA TCTTCCATGG TGGCTTTACC AACAGTACCG GATTGCCAAG
             61 CTTGGCCGCC GAGGCCAGAT CTTGATACCT CCGCTGGGGC TGAGTAGGAC TCAAGGATCC
            121 CAGACTTGTC CAATTATAGG GCTCCCCCTG TCCCGCCCCC TCCCCCAGGG ATAGGGCGGG
            181 CTGGGCCAGC CCCCAGTCAC GAGGTGGGCT GTTCTCCCCC TGCCCCAGGC ACAGCAGGGC
            241 AGAGGGAGGA AGGAGGTGGG GCATAGAGGT CTTTTGACCA CCCCCCACAG TCCCCAGGAA
            301 GGGAGGACAC CTCGCCCAGT AATACAGACA CCCTCCTCCA TTCTGGGGGC CAAGCGTGGA
            361 AGGGGAATGT GGAGGCTGCC GCCCAGAGTC CTCCTTTCCT GACCCTGTCC TTTCCTGTGC
            421 CTGGATGAAT GTAATCTGGA GAGGGGGCTG GGGATCTGGA CTCCTGGATC CCAGAAAGAA
            481 AGTAGGGCTA GGGGGCTGGA CAGAAGTGGG ATGGGAGGGG CTGGGGGCGG TAGCTCACGC
            541 CTGTAATCCC AGCACTTTGG GAGGCCACAG TGGGCGAATC ACTTAAGGTC AGGAGTTTGA
            601 GACCAGCCTG GCCAACATGG TGAAACCCCA TCTCTACTAA AAATACAAAA GTTAGCCAGG
            661 CGTGGTGGCG GGCGCCTGTA ATCCTAGCTA CTTGGGAGGC TGAGGCAGGA GAATCGCTTG
            721 AACCCGGGAG GCGGAGGTTA CAGTGAGCCG AGATCTCGCC ACTGCACTCC AGCCTCAGCA
            781 AGAGGGAGAC TGTCTCAAAA AAAGAAAAAA AAGAAAGTGG GGCTAGGCGG CTGGACAGAA
            841 GTGGGATGGG AGGGGTGGGA ACAGTATGAT GCACCTGCTA AAAGTCGAGG CTTTGCAAAT
            901 GGGACCTGGG TTTTAATCAC AGCTTCCTCT TTGGTTGTGA CCTTGAGCAC AGGGGAGCCC
            961 CCTCCCCCAC ATGTGAATGA GATTTCCAGC CACCCTCCCC CTGGCTGCCA CAGGTAATTG
           1021 TGCTTCCAAG GGACTGAGCC GTGCTTCTGG TAGGTAAGAA TCTGAGTCCA ATTGCAGGCG
          1081 TGATCCTGGC CTCCCTGCCA GATGC
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07556_A7Eoc.seq check
          >07556_07556_A7Eo_pAxCALNL_F1_C01_03_ABI08.ab1
              1 TTTAATTGTT ATCTTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC TCTAGAATTA
             61 TTACACGGCG ATCTTGCCGC CCTTCTTGGC CTTAATGAGA ATCTCGCGGA TCTTGCGGGC
            121 GTCCAACTTG CCGGTCAGTC CTTTAGGCAC CTCGTCCACG AACACAACAC CACCGCGCAG
            181 CTTCTTGGCG GTTGTAACCT GGCTGGCCAC ATAGTCCACG ATCTCCTTCT CGGTCATGGT
            241 TTTACCGTGT TCCAGCACGA CGACTGCGGC GGGCAGCTCG CCGGCATCGT CGTCGGGCAG
            301 GCCGGCGACC CCGGCGTCGA AGATGTTGGG GTGTTGCAGC AGGATGCTCT CCAGTTCGGC
            361 TGGGGCTACC TGGTAGCCCT TGTATTTGAT CAGGCTCTTC AGCCGGTCCA CGATGAAGAA
            421 GTGCTCGTCC TCGTCCCAGT AGGCGATGTC GCCGCTGTGC AGCCAGCCGT CCTTGTCGAT
            481 GAGAGCGTTT GTAGCCTCGG GGTTGTTAAC GTAGCCGCTC ATGATCATGG GGCCACGGAC
            541 GCACAGCTCG CCGCGCTGGT TCACACCCAG TGTCTTACCG GTGTCCAAGT CCACCACCTT
            601 AGCCTCGAAG AAGGGCACCA CCTTGCCTAC TGCGCCAGGC TTGTCGTCCC CTTCGGGGGT
            661 GATCAGAATG GCGCTGGTTG TTTCTGTCAG GCCGTAGCCC TGGCGGATGC CTGGTAGGTG
            721 GAAGCGTTTG GCCACGGCCT CACCTACCTC CTTGCTGAGC GGCGCCCCGC CGCTGGCGAT
            781 CTCGTGCAAG TTGCTTAGGT CGTACTTGTC GATGAGAGTG CTCTTAGCGA AGAAGCTAAA
            841 TAGTGTGGGC ACCAGCAGGG CAGATTGAAT CTTATAGTCT TGCAAGCTGC GCAAGAATAG
            901 CTCCTCCTCG AAGCGGGTAC ATGAGCACGA CCCGAAGCCG CAGATCAAGT AGCCCAGCGT
            961 GATGAACATG CCGGAAGCCG TGGTGAAATG CACCACGCTG AGGATAGCGG TGTTCGGGAT
           1021 GATCTGGGTT GGCCGGAGAT GGTGTTCGCG GTCCATGGAC CTGAATTCGA CCAGCGAGGC
          1081 GCATGCGGTT AGGCCTACGG CCTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl