RDB07556 - pGL4-phAPOE

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pGL4-phAPOE (#RDB07556)

Promoter collection, Human APOE promoter

Alternative name	U2839-30
Clone info.	PCR amplified human APOE promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 44904368 to 44905816(NC_000019.10); relatively -1428 to +21, where +1 corresponds to 1 nt of NM_001302688.2.
Comment	PCR cloning, forward primer: CCTTGCCAGCCAAAGCAAAC; reverse primer: ACCTCCGCTGGGGCTGAGTA. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human apolipoprotein E genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07556.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07556	pGL4-phAPOE	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phAPOE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07556).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07556_A7Eop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07556_A7Eoa.seq
>07556_07556_A7Eo_pGL4-4174F_A01_01_ABI08.ab1 1 AAAGGGTCAA ACTTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT 61 CCTTGCCAGC CAAAGCAAAC AGGCCCGGCC CGGCACTGGG GGTTCCTTCT CGAACCAGGA 121 GTTCAGCCTC CCCTGACCCG CAGAATCTTC TGATCCCACC CGCTCCAGGA GCCAGGAATG 181 AGTCCCAGTC TCTCCCAGTT CTCACTGTGT GGTTTTGCCA TTCGTCTTGC TGCTGAACCA 241 CGGGTTTCTC CTCTGAAACA TCTGGGATTT ATAACAGGGC TTAGGAAAGT GACAGCGTCT 301 GAGCGTTCAC TGTGGCCTGT CCATTGCTAG CCCTAACATA GGACCGCTGT GTGCCAGGGC 361 TGTCCTCCAT GCTCAATACA CGTTAGCTTG TCACCAAACA TACCCGTGCC GCTGCTTTCC 421 CAGTCTGATG AGCAAAGGAA CTTGATGCTC AGAGAGGACA AGTCATTTGC CCAAGGTCAC 481 ACAGCTGGCA ACTGGCAGAG CCAGGATTCA CGCCCTGGCA ATTTGACTCC AGAATCCTAA 541 CCTTAACCCA GAAGCACGGC TTCAAGCCCC TGGAAACCAC AATACCTGTG GCAGCCAGGG 601 GGAGGTGCTG GAATCTCATT TCACATGTGG GGAGGGGGCT CCCCTGTGCT CAAGGTCACA 661 ACCAAAGAGG AAGCTGTGAT TAAAACCCAG GTCCCATTTG CAAAGCCTCG ACTTTTAGCA 721 GGTGCATCAT ACTGTTCCCA CCCCTCCCAT CCCACTTCTG TCCAGCCGCC TAGCCCCACT 781 TTCTTTTTTT TCTTTTTTTG AGACAGTCTC CCTCTTGCTG AGGCTGGAGT GCAGTGGCGA 841 GATCTCGGCT CACTGTAACC TCCGCCTCCC GGGTTCAAGC GATTCTCCTG CCTCAGCCTC 901 CCAAGTAGCT AGGATTACAG GCGCCCGCCA CCCACGCCTG GCTAAACTTT TGTATTTTTT 961 AGTAGAGATG GGGGTTTCAC CATGTTTGGC CCAGGCTGGT CTCAAACTCC TGACCCTTTA 1021 AGTGATTTCG CCCACTGTTG CCTCCCAAAG TGCTGGGATA CAGCGTGAGC TACGTCCCCA 1081 GGCCCTCCAA TTCCCCACTT CCTTGTTCAA GGACTGT //
Primer: pGL4-136R Sequence file: RDB07556_A7Eob.seq
>07556_07556_A7Eo_pGL4-136R_B01_02_ABI08.ab1 1 GCTCTTATTG GTTTTTGGCA TCTTCCATGG TGGCTTTACC AACAGTACCG GATTGCCAAG 61 CTTGGCCGCC GAGGCCAGAT CTTGATACCT CCGCTGGGGC TGAGTAGGAC TCAAGGATCC 121 CAGACTTGTC CAATTATAGG GCTCCCCCTG TCCCGCCCCC TCCCCCAGGG ATAGGGCGGG 181 CTGGGCCAGC CCCCAGTCAC GAGGTGGGCT GTTCTCCCCC TGCCCCAGGC ACAGCAGGGC 241 AGAGGGAGGA AGGAGGTGGG GCATAGAGGT CTTTTGACCA CCCCCCACAG TCCCCAGGAA 301 GGGAGGACAC CTCGCCCAGT AATACAGACA CCCTCCTCCA TTCTGGGGGC CAAGCGTGGA 361 AGGGGAATGT GGAGGCTGCC GCCCAGAGTC CTCCTTTCCT GACCCTGTCC TTTCCTGTGC 421 CTGGATGAAT GTAATCTGGA GAGGGGGCTG GGGATCTGGA CTCCTGGATC CCAGAAAGAA 481 AGTAGGGCTA GGGGGCTGGA CAGAAGTGGG ATGGGAGGGG CTGGGGGCGG TAGCTCACGC 541 CTGTAATCCC AGCACTTTGG GAGGCCACAG TGGGCGAATC ACTTAAGGTC AGGAGTTTGA 601 GACCAGCCTG GCCAACATGG TGAAACCCCA TCTCTACTAA AAATACAAAA GTTAGCCAGG 661 CGTGGTGGCG GGCGCCTGTA ATCCTAGCTA CTTGGGAGGC TGAGGCAGGA GAATCGCTTG 721 AACCCGGGAG GCGGAGGTTA CAGTGAGCCG AGATCTCGCC ACTGCACTCC AGCCTCAGCA 781 AGAGGGAGAC TGTCTCAAAA AAAGAAAAAA AAGAAAGTGG GGCTAGGCGG CTGGACAGAA 841 GTGGGATGGG AGGGGTGGGA ACAGTATGAT GCACCTGCTA AAAGTCGAGG CTTTGCAAAT 901 GGGACCTGGG TTTTAATCAC AGCTTCCTCT TTGGTTGTGA CCTTGAGCAC AGGGGAGCCC 961 CCTCCCCCAC ATGTGAATGA GATTTCCAGC CACCCTCCCC CTGGCTGCCA CAGGTAATTG 1021 TGCTTCCAAG GGACTGAGCC GTGCTTCTGG TAGGTAAGAA TCTGAGTCCA ATTGCAGGCG 1081 TGATCCTGGC CTCCCTGCCA GATGC //
Primer: pAxCALNL_F1 Sequence file: RDB07556_A7Eoc.seq
>07556_07556_A7Eo_pAxCALNL_F1_C01_03_ABI08.ab1 1 TTTAATTGTT ATCTTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC TCTAGAATTA 61 TTACACGGCG ATCTTGCCGC CCTTCTTGGC CTTAATGAGA ATCTCGCGGA TCTTGCGGGC 121 GTCCAACTTG CCGGTCAGTC CTTTAGGCAC CTCGTCCACG AACACAACAC CACCGCGCAG 181 CTTCTTGGCG GTTGTAACCT GGCTGGCCAC ATAGTCCACG ATCTCCTTCT CGGTCATGGT 241 TTTACCGTGT TCCAGCACGA CGACTGCGGC GGGCAGCTCG CCGGCATCGT CGTCGGGCAG 301 GCCGGCGACC CCGGCGTCGA AGATGTTGGG GTGTTGCAGC AGGATGCTCT CCAGTTCGGC 361 TGGGGCTACC TGGTAGCCCT TGTATTTGAT CAGGCTCTTC AGCCGGTCCA CGATGAAGAA 421 GTGCTCGTCC TCGTCCCAGT AGGCGATGTC GCCGCTGTGC AGCCAGCCGT CCTTGTCGAT 481 GAGAGCGTTT GTAGCCTCGG GGTTGTTAAC GTAGCCGCTC ATGATCATGG GGCCACGGAC 541 GCACAGCTCG CCGCGCTGGT TCACACCCAG TGTCTTACCG GTGTCCAAGT CCACCACCTT 601 AGCCTCGAAG AAGGGCACCA CCTTGCCTAC TGCGCCAGGC TTGTCGTCCC CTTCGGGGGT 661 GATCAGAATG GCGCTGGTTG TTTCTGTCAG GCCGTAGCCC TGGCGGATGC CTGGTAGGTG 721 GAAGCGTTTG GCCACGGCCT CACCTACCTC CTTGCTGAGC GGCGCCCCGC CGCTGGCGAT 781 CTCGTGCAAG TTGCTTAGGT CGTACTTGTC GATGAGAGTG CTCTTAGCGA AGAAGCTAAA 841 TAGTGTGGGC ACCAGCAGGG CAGATTGAAT CTTATAGTCT TGCAAGCTGC GCAAGAATAG 901 CTCCTCCTCG AAGCGGGTAC ATGAGCACGA CCCGAAGCCG CAGATCAAGT AGCCCAGCGT 961 GATGAACATG CCGGAAGCCG TGGTGAAATG CACCACGCTG AGGATAGCGG TGTTCGGGAT 1021 GATCTGGGTT GGCCGGAGAT GGTGTTCGCG GTCCATGGAC CTGAATTCGA CCAGCGAGGC 1081 GCATGCGGTT AGGCCTACGG CCTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

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Other resources in our bank

human apolipoprotein E

External Database
Human apolipoprotein E

Reference sequence