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pGL4-phMMP9 (#RDB07537)

Promoter collection, Human MMP9 promoter

Alternative name U2792-2
Clone info. PCR amplified human MMP9 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 46007674 to 46009018(NC_000020.11); relatively -1234 to +111, where +1 corresponds to 1 nt of NM_004994.3.
Comment PCR cloning, forward primer: AGGGCTGGAGAACTGAAAGG; reverse primer: AAGAGCACAAGGGTGGACTG. Known difference : Deletion at 46,008,773 to 46,008,778. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07537.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07537 pGL4-phMMP9 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phMMP9 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07537).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14426_A6Kgp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB14426_A6Kga.seq check
>07537_A6Kg_4_pGL4-4174F_D11_11_ABI24.ab1
    1 ACTCAGGGGG CAAGAACATT TCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC
   61 GAGGATAGGG CTGGAGAACT GAAAGGGCTC CTATAGATTA TTTTCCCCCA TATCCTGCCC
  121 CAATTTGCAG TTGAAGAATC CTAAGCTGAC AAAGGGGAAG GCATTTACTC CAGGTTACAC
  181 TGCAGCTTAG AGCCCAATAA CCTGGTTTGG TGATTCCAAG TTAGAATCAT GGTCTTTTGG
  241 CAGGGTCTCG CTCTGTTGCC CAGGCTGGAG TGCAGTGACA TAATCATGGC TCACTGTATC
  301 CTTGACCTTC TTTCTGGGCT CAAGCAATCC TCCCACCTCG GCCTCCCAAA GTGCTAAGAT
  361 TACAGGAATG AGCCACCATA CCTGGCCCTG AATCTTGGGT CTTGGCCTTA GTAATTAAAA
  421 CCAATCACCA CCATCCGTTG CGGACTTACA ACCTACAGTG TTCTAAACAT TTTATATGTT
  481 TGATCTCATT TAATCCTCAC ATCAATTTAG GGACAAAGAG CCCCCCACCC CCCGTTTTTT
  541 TTTTTACAGC TGAGGAAACA CTTCAAAGTG GTAAGACATT TGCCCGAGGT CCTGAAGGAA
  601 GAGAGTAAAG CCATGTCTGC TGTTTTCTAG AGGCTGCTAC TGTCCCCTTT ACTGCCCTGA
  661 AGATTCAGCC TGCGGAAGAC AGGGGGTTGC CCCAGTGGAA TTCCCCAGCC TTGCCTAGCA
  721 GAGCCCATTC CTTCCGCCCC CAGATGAAGC AGGGAGAGGA AGCTGAGTCA AAGAAGGCTG
781 TCAGGGAGGG AAAAAGAGGA CAGAGCCTGG AGTGTGGGGA GGGGGTTTGG GGGAGG
//
Primer: pGL4-136R
Sequence file: RDB14426_A6Kgb.seq check
>07537_A6Kg_4_pGL4-136R_E11_14_ABI24.ab1
    1 GGTTTAGTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTTGG
   61 CCGCCGAGGC CAGATCTTGA TAAGAGCACA AGGGTGGACT GGCGCTGTCT GGGGGCAGCA
  121 AAGCAGCAGC CCAGCACCAG GAGCACCAGG ACCAGGGGCT GCCAGAGGCT CATGGTGAGG
  181 GCAGAGGTGT CTGACTGCAG CTGCTGTTGT GGGGGCTTTA AGGAGGCGCT CCTGTGACCC
  241 CACCCCTCCT TGACAGGCAA GTGCTGACTC AGGGGTCAGG GTGTGTGTGT GTGTGTGTGT
  301 GTGTGTGTGT GTGTGTGTGT GGCAGCACCA GCATGAGAAA GGGCTTACAC CACCTCCTCC
  361 TCTCTCCACT GCCCGCAGTC TCCACTGCCA AGTCAGGCAA GACCCCAGAC CCGCAGGAAA
  421 CCGCAGGCCC TCAGGGGAGG GGTAGGGTTT TGCAAACTGC AGAGCTTGTG GGAACTGTAT
481 GAAAGGGAGG GAGGGGGATG A
//
Primer: pAxCALNL_F1
Sequence file: RDB14426_A6Kgc.seq check
>07537_A6Kg_4_pAxCALNL_F1_F11_17_ABI24.ab1
    1 ATCAATGTAT CTATCATGTC TGCTCGAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA
   61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA
  121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT
  181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC
  241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG
  301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG
  361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT
  421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG
  481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA
  541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT
  601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA
  661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC
  721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT
  781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG
  841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT
  901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGGTGA
  961 ACATGCCGGA AGCCGTGGTG AAATGGCACC ACGCTGAGGG ATAGCGGTGT CGGGGATGAT
 1021 CTGGGTTGCC GAAGATTGGG GTCGCGGGCA TGACTGAATC GGGACACAGC GGTGCGGTGC
 1081 GGTAGGGGCT TACGGCCATG GCCATCCCAG GCAACTGCCA CTACCTGGTT TTCAATGGAA
1141 TTCAGGGGCT CGCGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles