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pGL4-phMMP3

Promoter collection, Human MMP3 promoter

Catalog number RDB07535
Resource name pGL4-phMMP3
Alternative name U2790-4
Clone info. PCR amplified human MMP3 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 102844952 to 102843589(NC_000011.10); relatively -1343 to +21, where +1 corresponds to 1 nt of NM_002422.5.
Comment PCR cloning, forward primer: CAACTGTGTGGGCATCTTCA; reverse primer: CTGTCTTGCCTGCCTCCTT. Known difference : G to C substitution at 102,843,985. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human matrix metallopeptidase 3 (stromelysin 1, progelatinase) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human matrix metallopeptidase 3 (stromelysin 1, progelatinase)

          Reference sequence
            

          Distribution information

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          RDB07535 pGL4-phMMP3 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07535.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14425_A6Kgp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB14425_A6Kga.seq check
          >07535_A6Kg_1_pGL4-4174F_A11_02_ABI24.ab1
              1 AGTGGGTCAG AACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
             61 ATCAACTGTG TGGGCATCTT CAGTCATAGG GATCTTATTG CCACAGAGAA ATCCCTTTAA
            121 ACTTATTGGG TAAAATCTCT CCAATGTTTA TTAAGAAACA CACAAAAAAT AAAGCAAAGA
            181 AGAAAATGCA AAAGAGTTAT AAATGAGAGG AAGCAAAATG GGCACTTATT AAAGGTCTAA
            241 TAAATGCACA TTTGTATCCA TCATTCTACT GAGTTCTTAC TCCCAAGATG TTCTTCCCTT
            301 TAGCAAACAA ATAAGCAAGT CAGCAAAGAA AGAAAGAACA AACAAAATGT GGTGATCAGG
            361 GAAGCATTGA GGAGATGGAT GGTGGCAGGT GGCAAGAGGA CTATAAAAGT TTTACAAAAT
            421 GTCTTCCTCT GAATATGTTT AGAGTCTTGC ATTCAAGCAT TTATTATACA CCAATAATGT
            481 GAGCAACACT TTACTTGACA AAGAAACAGA AAAGAAAGGA AAGGAAGAAA ACAGAAGAGC
            541 ATGAAGAGAA AATTTAGGAT GGATTCTGTT CTTCAACTTC AAAGCATCTG CTAATTTGAA
            601 TTTAGGGAGG AGGGGAAAAG GTTGAAAGAG AATAAGACAT GTGTAGAAGA CAAGGACAGA
            661 GAGAATTTCA GTCCGGTAAG CAATGTAATT CATTTCAATT CTACAACTAT TTTATGGAGC
          721 AGCTTCGTGG GCC
          //
          Primer: pGL4-136R
          Sequence file: RDB14425_A6Kgb.seq check
          >07535_A6Kg_1_pGL4-136R_B11_05_ABI24.ab1
              1 GGCCTATTTG TTTTGGGCAT CTTCCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC
             61 TTGGCCGCCG AGGCCAGATC TTGATCTGTC TTGCCTGCCT CCTTGTAGGT CCAACCTCGG
            121 GAGCGCAGCT TTTAAAGAGT GACAGTGTTT GTTTGGATCA CCCGCAGCTT GACTCATCCT
            181 TGCTTTCATC CAAATGGCAG CAGGACCATT TCCAAACATT CCAAATTCAA AGTAGGATGA
            241 TACAACCTAC TTTACTAATT TCTCTCAACC TTCCCAATTT TGCAAGGCAA CACAGTGATT
            301 AATCTCCAGG AAGTGCTTCC TGTCTTGGTA GAGGAGAAAA CTGGAGCATT TTTTTTCTTC
            361 TGGAAAAATT GACTGGAATT CACTAGACAA TTTATGTTTG TCCTTTTGAG AGAAGAAGTA
            421 GGTTGACTTG GTAAAAATCA GAATTGTGCA GAACTTCTAA AAGCAGTTTT ATTTTAAGAA
            481 AGACATTATA AAAGCAAATA AAAGATGTAA GTGGAAAGTG TACATACATA GCTATAGTCT
            541 ATACTATATA CAATTATACT CAAAACATAA AAGATAATAT CTAGAAGAAA TTTAGAAGGG
            601 GTGATTTATT TTTCTGAATC TGATTTGTAA TGCCTGCTAT TGACTGTCGT TGGATTTGCT
            661 GGTTCTTGAG GAGGAAAACC TCATGGGCAG AATAGAACAA AGAGGGTCTG TGAAAAAGAA
            721 GGAAGTATAT TCCCTTTGGG TTGGTTTTAA TTCTGTAACC AATTTATTAA TGGGTGATGG
            781 GCCCACGTAG CTGCTCCATA AATAGTTGTA GAATTGAAAT GAAATTACAT TGCTTACCGG
            841 ACTGAAAATT CTCTCTGTCC TTGTCTTCTA CACATGTCTT ATTTCTCTTT CAACCTTTTT
            901 CCCCTCCTCC CTAAATTTCA AATTAGCAGA TGCTTTGGAA GTTGAAGACA GAAATCCATC
            961 CTAAATTTTC TCTTCATGCT CTTTCTGGTT TTCTTCCTTT CCCTTTCTTT CTGTTCTTTG
           1021 GTCAAGTAAA GTGGTTGCCT CACATTAATT GGGTGTAATA ATTAAATGCT TTGGAAATGG
           1081 CAAGACTCTA ACATATTCAG AGGAAGAACT TTTTGTAAAA CCTTTAATAG GTCCGTTCTT
          1141 TGCCACAACC TGTGTGTCCA CACACTATC
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB14425_A6Kgc.seq check
          >07535_A6Kg_1_pAxCALNL_F1_C11_08_ABI24.ab1
              1 AACAATGATA TCTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT
             61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT
            121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT
            181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT
            241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC
            301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG
            361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT
            421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA
            481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC
            541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG
            601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA
            661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA
            721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT
            781 CGTGCAAGTT GCTTAGGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA
            841 GTGTGGGCAC CAGCAGGGCA GATTGAATCT TATAGTCTTG CAGGCTGCGC AAGAATAGCT
            901 CCTCCTCGAA GCGGTACATG AGCACGACCC GAAAGCCGCA GATCAAGTAG CCCAGCGTGG
            961 TGAACATGCC GAAAGCCGGT GGTTGAAATG GCACCCACGC TGAGGATAGC GTGTCGGGAA
           1021 TGATCTGGGT TTGCCGAAGA TGGGGTCGCG GGGCATTGAC CTGAAATCGG AAAACAAGCG
           1081 GTGCGGGTGC GGGTAGAGGC TACGCCCTTG GCGATCGGTT ACTTCCCACC TTACTTGGTC
          1141 TCAAGTGGAA TCTCACGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl