Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phMMP3 (#RDB07535)

Promoter collection, Human MMP3 promoter

Alternative name U2790-4
Clone info. PCR amplified human MMP3 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 102844952 to 102843589(NC_000011.10); relatively -1343 to +21, where +1 corresponds to 1 nt of NM_002422.5.
Comment PCR cloning, forward primer: CAACTGTGTGGGCATCTTCA; reverse primer: CTGTCTTGCCTGCCTCCTT. Known difference : G to C substitution at 102,843,985. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human matrix metallopeptidase 3 (stromelysin 1, progelatinase) genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07535.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 謝辞の表明を必要とする。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07535 pGL4-phMMP3 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phMMP3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07535).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14425_A6Kgp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB14425_A6Kga.seq check
>07535_A6Kg_1_pGL4-4174F_A11_02_ABI24.ab1
    1 AGTGGGTCAG AACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
   61 ATCAACTGTG TGGGCATCTT CAGTCATAGG GATCTTATTG CCACAGAGAA ATCCCTTTAA
  121 ACTTATTGGG TAAAATCTCT CCAATGTTTA TTAAGAAACA CACAAAAAAT AAAGCAAAGA
  181 AGAAAATGCA AAAGAGTTAT AAATGAGAGG AAGCAAAATG GGCACTTATT AAAGGTCTAA
  241 TAAATGCACA TTTGTATCCA TCATTCTACT GAGTTCTTAC TCCCAAGATG TTCTTCCCTT
  301 TAGCAAACAA ATAAGCAAGT CAGCAAAGAA AGAAAGAACA AACAAAATGT GGTGATCAGG
  361 GAAGCATTGA GGAGATGGAT GGTGGCAGGT GGCAAGAGGA CTATAAAAGT TTTACAAAAT
  421 GTCTTCCTCT GAATATGTTT AGAGTCTTGC ATTCAAGCAT TTATTATACA CCAATAATGT
  481 GAGCAACACT TTACTTGACA AAGAAACAGA AAAGAAAGGA AAGGAAGAAA ACAGAAGAGC
  541 ATGAAGAGAA AATTTAGGAT GGATTCTGTT CTTCAACTTC AAAGCATCTG CTAATTTGAA
  601 TTTAGGGAGG AGGGGAAAAG GTTGAAAGAG AATAAGACAT GTGTAGAAGA CAAGGACAGA
  661 GAGAATTTCA GTCCGGTAAG CAATGTAATT CATTTCAATT CTACAACTAT TTTATGGAGC
721 AGCTTCGTGG GCC
//
Primer: pGL4-136R
Sequence file: RDB14425_A6Kgb.seq check
>07535_A6Kg_1_pGL4-136R_B11_05_ABI24.ab1
    1 GGCCTATTTG TTTTGGGCAT CTTCCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC
   61 TTGGCCGCCG AGGCCAGATC TTGATCTGTC TTGCCTGCCT CCTTGTAGGT CCAACCTCGG
  121 GAGCGCAGCT TTTAAAGAGT GACAGTGTTT GTTTGGATCA CCCGCAGCTT GACTCATCCT
  181 TGCTTTCATC CAAATGGCAG CAGGACCATT TCCAAACATT CCAAATTCAA AGTAGGATGA
  241 TACAACCTAC TTTACTAATT TCTCTCAACC TTCCCAATTT TGCAAGGCAA CACAGTGATT
  301 AATCTCCAGG AAGTGCTTCC TGTCTTGGTA GAGGAGAAAA CTGGAGCATT TTTTTTCTTC
  361 TGGAAAAATT GACTGGAATT CACTAGACAA TTTATGTTTG TCCTTTTGAG AGAAGAAGTA
  421 GGTTGACTTG GTAAAAATCA GAATTGTGCA GAACTTCTAA AAGCAGTTTT ATTTTAAGAA
  481 AGACATTATA AAAGCAAATA AAAGATGTAA GTGGAAAGTG TACATACATA GCTATAGTCT
  541 ATACTATATA CAATTATACT CAAAACATAA AAGATAATAT CTAGAAGAAA TTTAGAAGGG
  601 GTGATTTATT TTTCTGAATC TGATTTGTAA TGCCTGCTAT TGACTGTCGT TGGATTTGCT
  661 GGTTCTTGAG GAGGAAAACC TCATGGGCAG AATAGAACAA AGAGGGTCTG TGAAAAAGAA
  721 GGAAGTATAT TCCCTTTGGG TTGGTTTTAA TTCTGTAACC AATTTATTAA TGGGTGATGG
  781 GCCCACGTAG CTGCTCCATA AATAGTTGTA GAATTGAAAT GAAATTACAT TGCTTACCGG
  841 ACTGAAAATT CTCTCTGTCC TTGTCTTCTA CACATGTCTT ATTTCTCTTT CAACCTTTTT
  901 CCCCTCCTCC CTAAATTTCA AATTAGCAGA TGCTTTGGAA GTTGAAGACA GAAATCCATC
  961 CTAAATTTTC TCTTCATGCT CTTTCTGGTT TTCTTCCTTT CCCTTTCTTT CTGTTCTTTG
 1021 GTCAAGTAAA GTGGTTGCCT CACATTAATT GGGTGTAATA ATTAAATGCT TTGGAAATGG
 1081 CAAGACTCTA ACATATTCAG AGGAAGAACT TTTTGTAAAA CCTTTAATAG GTCCGTTCTT
1141 TGCCACAACC TGTGTGTCCA CACACTATC
//
Primer: pAxCALNL_F1
Sequence file: RDB14425_A6Kgc.seq check
>07535_A6Kg_1_pAxCALNL_F1_C11_08_ABI24.ab1
    1 AACAATGATA TCTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT
   61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT
  121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT
  181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT
  241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC
  301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG
  361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT
  421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA
  481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC
  541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG
  601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA
  661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA
  721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT
  781 CGTGCAAGTT GCTTAGGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA
  841 GTGTGGGCAC CAGCAGGGCA GATTGAATCT TATAGTCTTG CAGGCTGCGC AAGAATAGCT
  901 CCTCCTCGAA GCGGTACATG AGCACGACCC GAAAGCCGCA GATCAAGTAG CCCAGCGTGG
  961 TGAACATGCC GAAAGCCGGT GGTTGAAATG GCACCCACGC TGAGGATAGC GTGTCGGGAA
 1021 TGATCTGGGT TTGCCGAAGA TGGGGTCGCG GGGCATTGAC CTGAAATCGG AAAACAAGCG
 1081 GTGCGGGTGC GGGTAGAGGC TACGCCCTTG GCGATCGGTT ACTTCCCACC TTACTTGGTC
1141 TCAAGTGGAA TCTCACGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles